phenotypic and functional diversity in tumor microenvironment

... structures In contrast, CCR5+ cells were detected mainly in the lining layer and in the sublining zone of the superficial subintima and, to a smaller extent, in the perivascular infiltrates of sublining ... aggregates in the sublining layer or scattered throughout the synovium up to the lining layer [20] Conversely, CCR5-positive cells were detected mainly in the lining layer and in the sublining zone ... field (see also the Methods section) Lining, lining layer and superficial subintima; sublining, perivascular infiltrates in sublining layer; aggregates, T and B cell aggregates with or without...

Ngày tải lên: 09/08/2014, 06:22

... not examine whether the BMDC changes were intrinsic defects or secondary to microenvironmental changes in the BM, including the cytokine milieu in our SLE patients This should be evaluated in future ... collection and analysis and to technical support All authors were actively involved in the drafting and the final approval of the manuscript Author Details 1Department of Medicine, Li Ka Shing Faculty ... effects in humans, in addition to using GM-CSF/IL4, we used FL to induce BMDC generation from both healthy donors and patients with SLE Morphologic and phenotypic analysis of control BM mDCs and...

Ngày tải lên: 12/08/2014, 12:20

... between before and after germination 38 ± 1.21 mg/g to 50 ± 2.16 mg/g (p < 0.01) Most storage proteins in rice grain are found in the endosperm, and brown rice contains about 83 mg/g protein (Matz, ... Fructose and sucrose were found before germination, and fructose and glucose found after germination Total free sugar content increased after germination Glucose which was absent in rough rice seed and ... different seed parts before and after germination are shown in Table Palmitic, oleic, and linoleic acids were the major fatty acids (80%), and stearic and linolenic acids were minor fatty acids The...

Ngày tải lên: 15/03/2014, 15:42

... single chain molecule The position of the binding domain of E could be obtained by rotating the binding domain of A or B about the linker region connecting the translocation and binding domains ... neurotoxins molecule more globular (Fig 12) In BoNT ⁄ A and B there are only limited interactions between the translocation and binding domains and no interaction between the binding and catalytic ... binding domain of BoNT ⁄ B The HCN domain has lectin binding motif and the HCC domain contains a b-trefoil fold which provides binding pockets for the receptors (1281–1314) at the C-terminus...

Ngày tải lên: 22/03/2014, 15:21

... academia and industry interested in the fundamental principles of polysaccharide functions and modications on one hand and novel applications on the other Having been involved in similar work mainly ... interdomain (interparticular) interaction becomes dominant, an interference peak will appear at the q range corresponding to the interaction distance in the scattering prole If the interdomain (interparticular) ... reaction time in the present system Table also indicates that about 3/4 of chains are involved in the quasi-ordered domain at a gel point, and more single chains are incorporated into the quasi-ordered...

Ngày tải lên: 23/05/2014, 13:14

... cultured in RPMI 1640 (Invitrogen) supplemented with 10% FCS (Hyclone), 1% Lglutamine and 1% Penicillin/Streptomycin (Gibco) in the presence of recombinant human Fms-like tyrosine kinase3-Ligand (FLT3-L; ... without inducing phenotypic and functional maturation [26] More recently, we also showed that self-complementary duplex strands but not single strands rAAV2/1 and were also very efficient in transducing ... E-GFP expressing pDC with desmin and C512 promoters were very high and equivalent to those obtained with PGK and EF1 promoters, respectively The average copy number in pDC for desmin and C512 promoters...

Ngày tải lên: 18/06/2014, 15:20

... preoperative arthritis and post operative Oxford score or medial knee pain Finally, we note that despite current interest in optimising the positioning of UKA to improve functional results, our ... discomfort or pain This was done in the postal enquiry by showing a diagram of a knee and asking patients to report where (if at all) they experienced persistent pain or discomfort by placing a cross ... revised within the 4.5 year follow up period and our outcomes in the surviving knees were disappointing compared with other studies [3,4,7,14,16-19], with 7.5% of our patients achieving ‘poor’...

Ngày tải lên: 20/06/2014, 07:20

... cells were obtained from MD-PBMC using peanut agglutinin (PNA) The cells were incubated on petri plates coated with PNA to remove αβ and WC1- γδ T cells and any remaining monocytes and nonadherent ... are prominent in normal bovine skin and express a diverse repertoire of antigen receptors Immunology, 1997, 91, 58-64 26 Hein, W.R and Mackay, C.R Prominence of τδ T cells in the ruminant immune ... selected time points and processed for FC and preparation of mRNA For sorting, the cells were labeled Phenotypic and functional analysis of bovine γδ lymphocytes Table List of mAbs used in this study...

Ngày tải lên: 07/08/2014, 14:22

... prevent root spiralling, and holes in the base facilitate drainage and encourage air pruning of roots Continual air pruning induces a limitation to the main root growth by shortening its length to ... (OSMOCOTE  Mini 18+6+11) was incorporated into the bulk substrate in a dose of 3.5 g L−1 Forty seedlings were cultivated in each container type according to a completely randomized design and using ... (n = 22) AR and VR were about 38% and 88% higher, respectively, in LT than in ST, whereas DR was about 50% higher in ST than in LT and DRW was about 50% higher in LT and ST than in MT Besides,...

Ngày tải lên: 08/08/2014, 00:22

... that gated CD24highand CD38high B cells express immunoglobulin M and immunoglobulin D (upper inset) and CD10 (lower inset) Immunohistochemical staining for IgG, IgA, and IgM in synovial tissue ... fixed in formalin 10%, and embedded independently in paraffin blocks or optimal cutting temperature (OCT) compound, and snap frozen in liquid nitrogen-cooled isopentane Immunohistochemical labeling ... Accordingly, we found an enrichment for IgG-secreting plasma blasts in SF and a large amount of IgG-producing B cells in synovial tissue The functional significance of these findings remains to...

Ngày tải lên: 09/08/2014, 14:22

... wild laboratory strain Oregon-R That strain was maintained in our laboratory at 25 °C for at least 15 years by transferring a hundred flies every third week into fresh half-pint milk bottles The ... was also made between the subpopulation 025 and the wild laboratory strain Bonlez (Belgium), recently caught in the wild and maintained since months) in the usual conditions, at 25 °C Table gives ... repetitive sequences with single copy DNA may be involved in the coordinate control of sets of functionally related genes during development and differentiation Can it then in the present case not...

Ngày tải lên: 09/08/2014, 22:22

... roles in angiogenesis: Ang-1 and Ang-4 are agonist ligands for Tie2 and induce tyrosin phosphorylation of Tie2, while Ang-2 and Ang-3 are antagonist ligands They bind to Tie2 without inducing tyrosin ... Ang-2 was mainly expressed in tumor stroma and had similar expression pattern in malignant and benign tumors (Figure 1C, D) Maspin expression was predominantly located in the cytoplasma and occasionally ... papillary cystadenocarcinoma; D: Ang-2 expression in ovarian borderline mucinous cystadenoma; E: Maspin expression in mucinous cystadenocarcinoma; F: Maspin expression in mucinous cystadenoma The...

Ngày tải lên: 10/08/2014, 10:21

... PDZ binding motif that may represent a virulence determinant PDZ domains are protein-interacting domains that are involved in a variety of cell signalling pathways In addition, Obenauer and colleagues ... determine whether some functional differences between the NS1 protein of mink/84 and chicken/49 isolates could explain the diversity in pathogenicity and virulence of these viruses in mink, a ... avian influenza viruses The H10 viruses have histidine (H), glutamate (E) and glutamine (Q) at amino acid positions 177, 184, and 216, respectively, in H10 numbering at the receptor-binding site...

Ngày tải lên: 12/08/2014, 04:20

... stooping, kneeling, getting up from a standing position, lifting lighter or heavier objects, standing, sitting, standing from a seated position, walking up stairs, and walking in the neighborhood ... pushing objects, like a living room chair? 13 14 In stooping, crouching, or kneeling? In getting up from a stooping, crouching, or kneeling position? In lifting or carrying items under 10 pounds, ... medication during a 12 month window beginning months before the index utilization date and ending months after index date (these medications included inhaled anticholinergic medications, inhaled beta...

Ngày tải lên: 12/08/2014, 15:20

... continual replenishing of the pool of infected resting CD4+ T lymphocytes harboring unintegrated HIV-1 DNA In sustained responder to HAART, the results obtained using the HIV-1 integrase inhibitor ... that the inducible functional preintegration reservoir was present in some individuals As observed in the model of latent infection and in untreated patients, this reservoir was functional and labile ... Characterization of preintegrated HIV-1 DNA using AluLTR real-time PCR in the model of latent infection and in infected patients In the model of latent infection as well as in two untreated patients...

Ngày tải lên: 13/08/2014, 05:22

... bacitracin, a PDI inhibitor Finally, PDI expression was induced by the antiinflammatory cytokine IL-10, and IL-10-mediated inhibition of LPS-induced IL-6 expression was reduced by bacitracin These findings ... adrenomedullin and adrenomedullin-binding protein-1 in sepsis: down-regulation of proinflammatory cytokines Crit Care Med 2002, 30:2729-2735 Darby NJ, Creighton TE: Functional properties of the individual ... molecular chaperones in the folding of oxidized proteins Refolding of colloidal thyroglobulin by protein disulfide isomerase and immunoglobulin heavy chain-binding protein J Biol Chem 2001, 276:21337-21342...

Ngày tải lên: 13/08/2014, 11:22

... homogeneity and the intensity of competition being positive in some cases (Perez-Tome and Toro, 1982; Ellstrand and Antonovics, 1985; Kelley et al, 1988; Martin et al, 1988) and negative in others ... j to + d do; and d being the number of individuals dying from ;, i ij + in the homogeneous and heterogeneous groups, respectively, and r = r + rl!, r and rl! being the number of individuals alive ... were obtained, individuals sharing the same vial being related at least as half-sibs In the second, eggs sired by all males were pooled, random samples of 150 being taken from the pool and introduced...

Ngày tải lên: 14/08/2014, 20:20

... pro-apoptotic activity of Bax and Bak by preventing the binding of these proteins to anti-apoptotic Bcl-2, suggesting that these pro-apoptotic proteins kill by binding and inhibiting their anti-apoptotic ... leading to cytochrome c release (Mihara, Erster et al 2003) Interestingly, this was achieved in the absence of a BH3 domain in p53, instead p53 binds to Bcl-2 using its proline-rich domain (Mihara, ... COX Va and COX Vb as cancer markers: 32 Interestingly, recent studies have demonstrated up-regulation and increased involvement of COX Va and Vb in a variety of cancers Autocrine gastrins-induced...

Ngày tải lên: 14/09/2015, 08:42

... steps These are objective finding, fact finding, problem finding, idea finding, solution finding and acceptance finding Each step is subdivided into a divergent thinking phase described as the ... within the company whose dominant functional background is in the ith functional area, with f functional areas Regarding the intrapersonal functional diversity (INTRA), the same kind of index ... within its dominant function does not affect the company dominant function diversity Since we intend to understand the impact of both dominant function diversity and intrapersonal functional diversity...

Ngày tải lên: 28/09/2015, 13:21

... synthesis, and inosine that will be degraded into hypoxanthine and xanthine and finally into uric acid Hypoxanthine and guanine may enter in a salvage pathway, using hypoxanthine-guanine phosphoribosyltranferase ... hypoxanyhine into xanthine and this latter into uric acid During this reaction an active metabolite, deriving by enzymatic action on allopurinol, oxypurinol, inhibits xanthine oxidase and probably ... half-life and further reduce immunogenicity The PEGylation consists in binding with a covalent link a protein (adenosine-deaminase, asparaginase, interferons, granulocyte colonystimulating factor,...

Ngày tải lên: 31/10/2012, 14:59