pg b6p mouse monoclonal antibody

... silkworms carrying cDNAs encoding a mouse IgG mAb We constructed two vectors, pIgGL ⁄ M1.1MG and pIgGH ⁄ M1.1R, for the generation of transgenic silkworms expressing a mouse IgG mAb (Fig 1) The former ... under reducing conditions, and analyzed by western blotting using polyclonal anti -mouse IgG serum Recombinant mouse IgG L- and H-chain was detected in the L- and H-lines, respectively (Fig 2A, ... stained with CBB, whereas others were subjected to western blotting using the rabbit anti (mouse IgG) as a primary antibody (western) ‘H2L2’, ‘H2L’, ‘H2’, ‘H’ and ‘L’ to the right of the gel indicate...

Ngày tải lên: 23/03/2014, 04:20

... motavizumab We previously showed that the superior neutralizing activity of this anti-RSV monoclonal antibody compared with palivizumab was associated with further reductions in RSV replication ... inoculation (1.25 mg in 0.1 ml of PBS/per mouse) [5] Our previous studies showed that no treatment or treatment with either PBS or an IgG1 isotypematched control antibody, MEDI-507, at the same time ... modified with the use of a specific anti-RSV neutralizing antibody directed against a well conserved epitope of the RSV F protein In summary, in the mouse model, prophylactic administration of motavizumab...

Ngày tải lên: 18/06/2014, 18:20

... motavizumab We previously showed that the superior neutralizing activity of this anti-RSV monoclonal antibody compared with palivizumab was associated with further reductions in RSV replication ... inoculation (1.25 mg in 0.1 ml of PBS/per mouse) [5] Our previous studies showed that no treatment or treatment with either PBS or an IgG1 isotypematched control antibody, MEDI-507, at the same time ... modified with the use of a specific anti-RSV neutralizing antibody directed against a well conserved epitope of the RSV F protein In summary, in the mouse model, prophylactic administration of motavizumab...

Ngày tải lên: 20/06/2014, 01:20

... synthesized in the second step Decapeptides (SKICEPGYSP), (ICEPGYSPTY) and (EPGYSPTYKQ) at spots 1, and 3, respectively, possessing the common EPGYSP motif reacted positively with 2A2 mAb (C) Structure ... possessing the EPGYSP sequence, reacted positively with 2A2 mAb (Fig 2B) In control experiments, primary antibody was omitted from the assay (data not shown) The epitope sequence EPGYSP is located ... octapeptides, KCSAICEP and SAICEPGY, were tested for binding to 2A2 mAb They contain the EP and EPGY sequences, respectively, of the predicted epitope sequence EPGYSP 2A2 mAb showed no binding...

Ngày tải lên: 18/02/2014, 11:20

... Ó FEBS 2003 A PAI-1-protecting monoclonal antibody (Eur J Biochem 270) 1673 Fig Localization of the epitope for Mab-1 in the three-dimensional ... 0.01) and Q58/74AD307/318A (15 ± 3%; n ¼ 3; P < 0.01) Monoclonal antibodies Mab-1 was produced and purified as described previously [29] Two other monoclonal antibodies against PAI-1, Mab2 [29,36,37] ... in a volume of 100 lL, with or without antibody (4 lgÆmL)1 Mab-1 or 80 lgÆmL)1 Mab-5) The dilution series were then incubated for 10 at 37 °C to allow antibody PAI-1 complex formation Fifty-lL...

Ngày tải lên: 20/02/2014, 11:20

... Ó FEBS 2002 short sugar side chains (e.g T, ST and TF antigens) [8,9] The monoclonal antibody SM3 was raised in mice against partially deglycosylated human MUC-1 It shows a high ... to the antibody [18] Conventional pepscan analysis however, does not allow easy analysis of the contribution of the carbohydrate portion To assess the involvement of carbohydrates in antibody ... MUC-1 peptide PDTRP and the MUC-1 glycopeptide PDT(O-aD-GalNAc)RP (cf Figure 1) bound to the monoclonal antibody SM3 MATERIALS AND METHODS Chemicals Chemicals for peptide synthesis were obtained...

Ngày tải lên: 21/02/2014, 15:20

... with a : 1000 dilution of HRP-conjugated rabbit anti- (mouse polyclonal IgG) Monoclonal antibody preparation SDS ⁄ PAGE and western blotting Monoclonal antibodies directed against FnBRB were raised ... with rabbit polyclonal antibody against Fn followed by addition of peroxidase-conjugated goat anti-(rabbit IgG) Monoclonal antibodies against FnBRs of FnBPB A panel of mouse mAbs was produced ... lL) and probed with 100 lL of 10 lgÆmL)1 15E11 Bound antibody was detected by incubating with secondary antibody [HRP-conjugated rabbit anti- (mouse IgG)] (B, D) Western blot Purified amounts (8...

Ngày tải lên: 06/03/2014, 22:21

... the Pk antigen as determined by a monoclonal antibody J Immunogenet 12, 213–220 32 Oosterwijk, E., Kalisiak, A., Wakka, J., Scheinberg, D & Old, L.J (1991) Monoclonal antibodies against Gala1–4Galß1–4Glc ... blocker and 1% (v/v) NGS/TBS as above, and stained with mouse anti-SGC SULF1 (2 lgÆmL)1) followed by incubation with HRP-conjugated goat anti -mouse Ig (1 : 500) Each step consisted of a 30 incubation ... Fellows, M & Tursz, T (1983) A glycolipid antigen associated with Burkitt lymphoma defined by a monoclonal antibody Science 220, 509 Li, S.-C., Kundu, S.K., Degasperi, R & Li, Y.-T (1986) Accumulation...

Ngày tải lên: 16/03/2014, 16:20

... to test the clones in an ELISA Conditioned media were collected from cloned mouse myeloma cells cultured in a TECNOMOUSE (Integra Biosciences, Wallisellen, Switzerland) hollow fiber chamber, and ... animals Binding of 125 I-labeled T–AT complex to HepG2 cells The binding studies were performed, as previously described, with minor modifications [21] HepG2 cells were cultured at 37 °C, 5% CO2 in 75-cm2 ... of T–AT to the SEC receptor in a less complex system, HepG2 cells were incubated with radiolabeled T–AT in the presence and absence of the antibody The binding of radiolabeled T–AT was reduced...

Ngày tải lên: 17/03/2014, 10:20

... Study of the antigenic determinant of PGM34 by modification of mucin PGM34 was developed using pig gastric mucin as an antigen To characterize the epitope of PGM34, periodate oxidation and trypsin ... mucin are involved in the epitope of PGM34 Reactivity of PGM34 with oligosaccharides obtained from pig gastric mucin For characterization of the epitope of PGM34, reduced oligosaccharides were ... with PGM34 (Fig 6B) Fraction IV, which reacted with PGM34, was the original A1-5b as determined from the mass and retention time on HPLC Fraction I had no Fuc residue and did not react with PGM34...

Ngày tải lên: 23/03/2014, 09:21

... Although monoclonal DPP IV antibody 4D10 arrests the cell cycle FEBS Journal 276 (2009) 6548–6559 ª 2009 The Authors Journal compilation ª 2009 FEBS 6555 Epitope of monoclonal DPP IV antibody ... overnight with lg of 6A3, control, or human DPP IV (hDPP IV) mAb from mouse and then to immunoblotting with hDPP IV mAb IF7 from mouse (D) Mouse, rat kidney extracts or DPP IV-Jurkat cell lysates (made ... compilation ª 2009 FEBS T.-T Hung et al Epitope of monoclonal DPP IV antibody A IP: B l tro on 6A3 C U3 C PP l tro IgG on Rat C 6A IP: 110 kDa IB: 6A3 Mouse Kidney Extract Rat Kidney Extract C IP:...

Ngày tải lên: 29/03/2014, 22:21

... study with an antibody known to inhibit binding of hTF to TFR to delineate the residues that comprise the antibody binding site and thus, by extension, the TFR binding site The E coli pGEX expression ... including the pGEX 4T3 control Furthermore, the hTF C-lobe, hTF-5C and hTF-5D (lanes 4, 10 and 12) samples had additional bands at lower molecular weights not seen in the pGEX 4T3 control ... human TF while showing no cross-reactivity with pig and mouse TF [24] Discussion The present study establishes that the epitope of the F11 antibody is in the C-lobe of hTF, specifically within residues...

Ngày tải lên: 30/03/2014, 11:20

... Vitro Methods 23 Advantages of Mouse Ascites Method 24 Disadvantages of Mouse Ascites Methods 24 Large-Scale Production of Monoclonal Antibodies 25 Monoclonal Antibody Production for Diagnostic ... Lines Secreting Monoclonal Antibodies In Vitro Production of Monoclonal Antibody 12 Batch Tissue-Culture Methods 12 Semipermeable-Membrane-Based Systems 14 Scientific Needs for Mouse Ascites Production ... quantities of mAb are used • The mouse ascites method avoids the need to teach the antibody producer tissue-culture methods Disadvantages of Mouse Ascites Method • The mouse ascites method involves...

Ngày tải lên: 11/04/2014, 07:16

... For example, tf the antigen dose per injection for a mouse is 100 pg, then a stock solution of (50 x 100 or) 5000 pg/ mL is desirable, Because a mouse dose will be contained in 0.2 mL, this solution ... quality of antibody, the immunization protocol should be designed to yield the maxtmum number of antibody- forming cells from the spleen Experience has shown that a mouse with a higher serum antibody ... produce homogenous antibody at a high rate Mouse- human heterohybridomas are generally not difficult to produce and have about the same in vitro growth characteristics as the mouse- mouse hybridomas...

Ngày tải lên: 11/04/2014, 09:52

... A/CK/Indonesia/R60/05 a assays performed using humanized VN04-2 antibody at ug/mL data taken from Chen et al [11] performed using the mouse VN04-2 antibody (15A3) of unknown concentration; data obtained ... human monoclonal antibodies against H5N1 influenza PLoS Med 2007, 4:e178 Hanson BJ, Boon AC, Lim AP, Webb A, Ooi EE, Webby RJ: Passive immunoprophylaxis and therapy with humanized monoclonal antibody ... the 140s loop may be the main determinants of antibody binding for VN042, but residues outside of this region may also contribute to the overall antibody binding affinity Previous studies with...

Ngày tải lên: 20/06/2014, 01:20

... have reported the therapeutic efficacy of passive immunization in a HPAI H5N1 mouse model with either humanized mouse mAb [14], equine F(ab')2 [15], or human mAb [16], directed against hemagglutinin ... groups A representative of each of these groups was selected to be cloned for use in antibody screening and antibody binding analysis (Table 1) Full-length HAs were constructed by replacing the ... conjugated) was used as the secondary signal producing antibody 1-Untreated IN597-FO; 2-Trypsin-cleaved IN597-FO The humanized protective antibody VN04-2 was used as a positive control for presence...

Ngày tải lên: 20/06/2014, 01:20

Ngày tải lên: 07/08/2014, 17:22

... setalosi suoirav fo noitcaer tset noitanitulgga-oC iloc E iloc E elba T la te yenhsra V C hsejarB 26 .PG rawlaT ,P nahtookiallihT ,P dnahC ,VH artaB 315-505 ,102 ,2891 J mehcoiB noitaziretcarahc rieht...

Ngày tải lên: 07/08/2014, 20:23

... human leukocyte antigen B27 transgenic rats treated with control assays monoclonal antibody IgG (filled bars) or monoclonal antibody C11C1 (open bars) at day 16 of the experimental protocol Values ... grown in a nude (immunodeficient) mouse model [47], we used this fourth approach in the present study This antibody has recently been successfully employed in the PG- APS model in which mAb C11C1 ... inflammation Effect of mAb C11C1 on HLA-B27 transgenic rats colonic inflammation (a) Effects of monoclonal antibody (mAb) C11C1 on diarrhea in human leukocyte antigen B27 (HLA-B27) rats Stool score...

Ngày tải lên: 09/08/2014, 06:22

... influence of dose Therefore, these data overall raise concerns about the safety of anti-TNF monoclonal antibody therapy in RA, especially when used at high doses Commentary However, there are a ... the BSR References Bongartz T, Sutton AJ, Sweeting MJ, Buchan I, Matteson EL, Montori V: Anti-TNF antibody therapy in rheumatoid arthritis and the risk of serious infections and malignancies: systematic...

Ngày tải lên: 09/08/2014, 08:22