pestis and y enterocolitica

... analysis Unless otherwise indicated, we carried out a bootstrap analysis to compare SAVs against the hSNP set by randomly sampling sets of the same size and proportion of synonymous and non-synonymous ... D, He M, Berry SA, Rinaldo P, Whitley CB, Madsen PP, van Calcar SC, Lussky RC, Andresen BS, Wolff JA, Vockley J: Prospective diagnosis of 2methylbutyryl-CoA dehydrogenase deficiency in the Hmong ... the scoring matrix not only preferentially scores synonymous over non-synonymous positions, but also incorporates other influences, such as codon bias and hypermutability For example, the highest...

Ngày tải lên: 09/08/2014, 20:21

... ml/min Bound IgY was eluted by chaotropic salt and immediately dialyzed with PBS The recovered IgY checked at 280 nm was close to mg/ml As shown in Fig 2, IgY showed a very high purity and did not ... column and T-gel column [9, 24] By analyzing purified IgY with SDS-PAGE, we found that the band of IgY light chain was weaker than that of IgY heavy chain, which pattern was previously reported by ... were produced by immunizing BALB/c mice against 50 µg/ml of flagella and fusing equal numbers of splenic lymphocytes and murine myeloma SP2/0 cells as previously described by Kohler and Milstein...

Ngày tải lên: 07/08/2014, 18:21

... bioanalytical systems that are simple and yet highly sensitive With the availability of small, easy-to-carry thermal cyclers, this assay could be developed as a portable assay which may cater ... colony forming units (CFU), respectively, and days post-challenge However, fecal samples collected 6, 8, and 10 days after challenge were found to be negative by both the biosensor assay and MAP ... Briefly, 40.3 μmol dipalmitoyl phosphatidyl choline, 21 μmol dipalmitoyl phosphatidyl glycerol, and 51.7 μmol cholesterol were dissolved in a mixture of chloroform, methanol, and isopropyl ether...

Ngày tải lên: 07/08/2014, 23:22

... results obtained by analysis of faeces and sera from 176 pigs in 24 herds collected during two previous studies, A and B, and analysed by nested PCR and by two serological methods, ELISA and IFAT PCR ... respectively, and employing a cutoff value of PI 35, the estimated sensitivity was 0.72 and the specificity was 0.93, with 95% probability intervals 0.59 - 0.83 and 0.83 - 0.99, respectively The median ... probability intervals 0.63 - 0.87 and 0.51 - 0.73, respectively Employing a cut-off value of PI 30, the estimated sensitivity and specificity was 0.75, with 95% probability intervals 0.63 0.86 and...

Ngày tải lên: 12/08/2014, 18:22

... of symptomatic patients suspected to suffer from coronary disease/ischemia is discussed Key words: coronary artery disease, ECG analysis, Coronary Artery Stenosis Introduction Coronary artery disease ... proposed and developed to enhance sensitivity and specificity of the resting ECG for diagnosis of symptomatic and asymptomatic CAD In theory, such methods may improve diagnostic quality for non-specialists ... Combined Analysis Total USA Asia Germany female male < 65 years 65+ years Female, < 65 years Female, 65+ years Male, < 65 years Male, 65+ years No Revasc PCI CABG Revasc of any type ROC AUC Odds...

Ngày tải lên: 03/11/2012, 10:58

... intracellular and intercellular dynamics in yeast glycolytic oscillations Biophys J 78, 1145–1153 Tyson, J.J & Novak, B (2001) Regulation of the eucaryotic cell cycle: molecular antagonism, hysteresis, and ... In Fig 1, two enzymes, E1 and E2, modify each other by releasing group P in the reactions E2-P fi P + E2 and E1-P fi P + E1 The former reaction is catalyzed by E1, the latter by E2 The arrows indicate ... Reaction may be coupled to a source of external free energy Alternations between two enzyme activities are caused by conformational transitions, induced by the modifying enzymes, E3 (catalyzing reaction...

Ngày tải lên: 19/02/2014, 16:20

... TCA cycle and amino acid turnover Mol Cancer 7, 72 Lu Y, Jiye A, Wang G, Hao H, Huang Q, Yan B, Zha W, Gu S, Ren H, Zhang Y et al (2008) Gas chromatography ⁄ time-of-flight mass spectrometry based ... with the study by Lu et al [9], which reports metabolic variation related to hypertension and age-related conditions To characterize the development of hypertension, the spontaneously hypertensive ... et al [25,26] goes beyond gene pairs and fully captures the topology of signaling pathways by propagating the perturbations measured at gene levels through the entire pathway This technique can...

Ngày tải lên: 16/03/2014, 01:20

Ngày tải lên: 19/03/2014, 00:20

... 95044 and lymphocytes from a baboon (Papio cynocephalus) (Pcy78404) were kindly provided by H Bielefeldt-Ohmann and C.-C Tsai, respectively, from the WaNPRC DNA from the PBMC of thirty random ... (Macaca fascicularis) and the lymphocytes of a baboon (Papio cynocephalus) The standard RV2 assay had an amplification efficiency less than 100% with the M fascicularis and P cynocephalus templates ... of thirty macaques housed at the WaNPRC and analyzed using the standard RV2 and OSM QPCR assays While all of the samples were positive for the single copy OSM gene, only six of the thirty macaques...

Ngày tải lên: 18/06/2014, 22:20

... 95044 and lymphocytes from a baboon (Papio cynocephalus) (Pcy78404) were kindly provided by H Bielefeldt-Ohmann and C.-C Tsai, respectively, from the WaNPRC DNA from the PBMC of thirty random ... (Macaca fascicularis) and the lymphocytes of a baboon (Papio cynocephalus) The standard RV2 assay had an amplification efficiency less than 100% with the M fascicularis and P cynocephalus templates ... of thirty macaques housed at the WaNPRC and analyzed using the standard RV2 and OSM QPCR assays While all of the samples were positive for the single copy OSM gene, only six of the thirty macaques...

Ngày tải lên: 20/06/2014, 04:20

... with analogy bright-field and fluorescent images with high optical quality Furthermore, Si3N4 features minimal auto-fluorescence in comparison to polystyrene Currently, only few microsystems exist ... GFP intensity) and its frequency (y- axis: frequency) is presented varies from cell to cell, e g., depending on the cell cycle stage or on previous exposures Therefore, the analysis and quantification ... inflammatory cytokine, and consequently, the intensity of the IL8 promoter activation is proportional to the GFP expression, which could be quantified microscopically and fluorometrically For fluorometric...

Ngày tải lên: 21/06/2014, 00:20

... primary and secondary data When the matrices R and C are estimated from both the K primary and the K’ secondary data, and assuming that the samples bTv(nT)’ (0 ≤ n ≤ K’ − 1) also verify assumptions ... secondary data only When the matrices R and C are estimated from the secondary data only, assuming K’ ≥ 2N (to ensure the invertibility of (24)) and the samples bTv(nT)’ (0 ≤ n ≤ K’ − 1) also verify ... secondary data When the matrices R and C are estimated from both the K primary and the K’ secondary data, and assuming that the samples bTv(nT)’ (0 ≤ n ≤ K’ − 1) also verify assumptions A1, A2 and...

Ngày tải lên: 21/06/2014, 00:20

... a good acceptability for any average lab assistant Table Comparison between QDs and superparamagnetic nanoparticle-based hybridization and type-specific PCR Hybridization Type-specific PCR Sum ... assays in such a way that the reaction occurs at homogeneous solution and that of conventional hybridization assay bases on the solid supporter such as polyvinylidene fluoride membrane or Yu-Hong ... 78:3234-3241 22 Lin CY, Chao A, Yang YC, Chou HH, Ho CM, Lin RW, Chang TC, Chiou JY, Chao FY, Wang KL, Chien TY, Hsueh S, Huang CC, Chen CJ, Lai CH: Human papillomavirus typing with a polymerase chain...

Ngày tải lên: 21/06/2014, 01:20

... SE FV SE FV FV FV SE FV FV SE FV CC SE 5IY 4 1Y 3IY 1SA 7IY 2YB 7SA 9SA 4SA 2TP 2CJ 2IY 1TP 6IY 1IY 1YB 1CJ RCP-TR RCP-TR *elpmas noitpircsed T/R yb fo epyT elpmaS seulav tC lanoitnevnoC VDMF 2002/RKS/O ... tnereffid gnitset yb denimreted saw RCP-TR T/R eht fo yticificeps lacitylana ehT yticificepS yassa RCP-TR lanoitnevnoc naht ytivitisnes rehgih dlof 0001 a dah yassa RCP-TR T/R ehT ASILE-gA yb detset ... naMqaT gnizidirbyh yllacificeps a fo sisylordyh detaidem-esaremylop qaT eht htiw detaicossa niag ecnecseroulf eht yb derotinom si noitacifilpma ,RCP-TR T/R naMqaT nI cificeps VDMF yltneiciffus erew...

Ngày tải lên: 07/08/2014, 18:21

... selected by the keywords 'inflam', 'T cell', 'lymphocyte' and 'cytokine' IL22 activates three different STAT genes: STAT1, STAT3 and STAT5 [13] RA synovial fibroblasts are relatively resistant ... obvious gene candidate, (2) likely gene candidate, (3) possible gene candidate, (4) unlikely gene candidate and (5) gene without relevance In addition, the genes that were ranked as high by the CGC ... phenotypes Typically, a genomic region might be known to be associated with a phenotype, but still there are usually many genes within such a region that might be possible candidates Specifically,...

Ngày tải lên: 09/08/2014, 06:22

... genetic maps may not be available for all species; systematic use of molecular markers is very costly; iii) statistical analysis of ii) phenotype distributions is a useful preliminary analysis of available ... were described by Mode and Gasser (1972) and Weber (1959) The power of this type of experiment has been recently investigated by Janss and Van der Werf (1992), limiting their study to the case ... (BC1) and AB or BB animals (BC2) with proportions 1/2, 1/2 The statistical analysis of the data obtained from these populations was clearly described by Elston and Stewart (1973) and Stewart and...

Ngày tải lên: 09/08/2014, 18:21

... iC yc kA yc yv IB oI m sp fh C lC co fo G F C B E D G G G G G m om om om om co c c c c m yh yh uC rP yv A G m co zC xC yq rO yv af m ys zG qzE y rN yv xA re m yq (e) rM yv K co kB yc xD kC yh yh ... yv K co kB yc xD kC yh yh kD yc xL yq jB jA yh yc G m co kE yc n ni cA nu C tlp kF yc kG yc kH yc Demonstration of the power of visualization in transcriptome analyses Figure Demonstration of ... in Figure 2b yvrP, yvrN and yvrM were found to be ComK-activated, whereas yvrO did not meet the criteria [24] Visualization in Genome2D reveals that yvrO is also slightly activated, and allows...

Ngày tải lên: 09/08/2014, 20:20

... reliability and poor to moderate inter-rater reliability, compared to excellent intra-rater and inter-rater reliability in the current study Superior reliability in the current study may have resulted ... reliability, and this along side consideration of validity for further use clinically and in research will now be discussed Item Fit Inadequately sized shoes have been reported in between 72 and 81% ... 1.00 [20]) and inter-rater (0.93 versus 0.80 to 0.90 [20]) reliability to that of Menz and Sherrington [20] for categorising footwear type Improved reliability in the current study may have resulted...

Ngày tải lên: 10/08/2014, 21:23

... audience, who may have varying levels of skills and experience in quality improvement and research Third, NorthStar has to be simple to read and navigate to ensure its relevance, acceptability and usefulness ... examines how to identify and select priorities in order to use resources efficiently (Priority Setting) Next, it provides guidance on how to identify, critically appraise, adapt, and develop relevant ... are and how to identify them using both explorative methods and theory-based methods (Identifying Determinants of Practice) It then provides guidance on what intervention strategy to use, and...

Ngày tải lên: 11/08/2014, 05:22

... Plant metabolomics and its potential for systems biology research: background concepts, technology and methodology In Methods in Systems Biology Edited by: Westerhoff H, Hayes N Elsevier Inc; ... similarity between genetically modified and conventional potato crops Proc Natl Acad Sci USA 2005, 102:14458-14462 Hirai MY, Klein M, Fujikawa Y, Yano M, Goodenowe DB, Yamazaki Y, Kanaya S, Nakamura Y, ... carbohydrates, lipids and diverse secondary metabolites, such as phenylpropanoids, isoprenoids, terpenoids and alkaloids) simultaneously through a single extraction and with only one analytical...

Ngày tải lên: 11/08/2014, 11:21