perseverance and patience are essential

... Dnag2-I and Dnag2-II are Dnag2 strains C2332 and A523, and nag1Dnag2-I and nag1Dnag2-II are Dnag1Dnag2 strains 713 and 1921, respectively Chitin degradation in Trichoderma atroviride NAG1 and NAG2 are ... NAGases are not essential for normal growth on non-chitinous carbon sources Dnag2 strains C2332 and A523 and Dnag1Dnag2 strains 713 and 1921 were randomly chosen and used together with the WT and ... normal growth and sporulation of the knockout strains, and (C) minimal medium with chitobiose Dnag2-I and Dnag2-II are Dnag2 strains C2332 and A523, and nag1Dnag2-I and nag1Dnag2II are Dnag1Dnag2...

Ngày tải lên: 23/03/2014, 05:22

... acids and agar were from BD (Franklin Lakes, NJ, USA) Ammonium sulphate and amino acids were from Merck (Darmstadt, Germany) Strains, plasmids and primers The strains and plasmids used are listed ... Leu2F and Leu2R (supplementary Table S1) These two primers contained 20 nucleotides that matched the 5¢- and 3¢-sequence of LEU2, and 40 nucleotides with a sequence identical to the 5¢- and 3¢-sequences ... mm Mg(CH3COO)2, 100 mm KCl and mm dithiothreitol, and suspended in the same buffer containing mm PMSF and 4000 U RNasine The cell suspension was mixed with glass beads and the yeast cells lysed...

Ngày tải lên: 07/03/2014, 05:20

... exposed and unexposed to the O2 and during recovery under normoxic conditions Data analysis Data are reported as means standard deviations (SDs), except in Fig 4B,C, where means standard errors ... were treated for 20 with 10 units of Cat and EhPFOR was reactivated for 30 at C with mM Fe2+ under reducing and anaerobic conditions For (A) and (C), data are mean SD; 100% activity is as in Fig ... centrifuged and resuspended in normoxic (0.18 0.09 mM O2 at 36 C and 2240 m altitude) NaCl Pi + glucose and returned to the water bath At different time intervals, samples were centrifuged, and enzyme...

Ngày tải lên: 15/03/2014, 23:20

... harmony between what they expect and what they are able to realize from life But suppose the pains of separation are amplified by a combination of parental demands and individual needs to the degree ... dependency and a longing for good and heroic parents? Or is it true that no matter how competent managers are, their leadership stagnates because of their limitations in visualizing purposes and generating ... arise out of necessities rather than desires and, therefore, are deeply embedded in their organization’s history and culture Frederic G Donner, chairman and chief executive officer of General Motors...

Ngày tải lên: 24/10/2013, 07:15

... Represents commands, command options, and portions of syntax that must be typed exactly as shown It also indicates commands on menus and buttons, icons, dialog box titles and options, and icon and menu ... following areas: information sharing, hardware and software sharing, and administrative support These benefits help increase productivity „# Information sharing The ability to share information and ... there are no dedicated servers, and there is no hierarchy among the computers All computers are equal and therefore are known as peers Each computer functions as both a client and a server, and...

Ngày tải lên: 09/12/2013, 17:15

... thyroid gland is large and firm Her face is puffy, her hands and feet feel cold to the touch and her reflexes are slow The doctor suggests that Zadie may have a problem with her thyroid gland Q1 What ... case, you should be able to: • understand and describe the signs and symptoms of the disorder in question and its underlying pathophysiology; • understand and describe the pharmacology of agents ... conditions and that care must be taken to consider all the factors presented before formulating your answers or coming to a conclusion about the case study Key points are provided for each case and are...

Ngày tải lên: 17/01/2014, 06:20

... thyroid gland is large and firm Her face is puffy, her hands and feet feel cold to the touch and her reflexes are slow The doctor suggests that Zadie may have a problem with her thyroid gland Q1 What ... case, you should be able to: • understand and describe the signs and symptoms of the disorder in question and its underlying pathophysiology; • understand and describe the pharmacology of agents ... conditions and that care must be taken to consider all the factors presented before formulating your answers or coming to a conclusion about the case study Key points are provided for each case and are...

Ngày tải lên: 17/02/2014, 05:20

... the apparent first-order rate constants k1 and k2 mimic Src activation and deactivation These (de)activation processes are brought about by stimulation of a plethora of cellular receptors and signaling ... activity are likely to occur when the activities of both activatory phosphatase (RPTP) and inhibitory kinase (Csk) are high, whereas Src oscillations may occur when these activities are low (Figs and ... values, 117.5, 126.5 and 135.5 nM, are indicated by dashed lines 1–3 in (A) and shown by upper line in (B) The catalytic efficiency of RPTP (steps and 6) is kcat/ KM = 3.6 · 10)3 and 0.02 nM)1Æs)1);...

Ngày tải lên: 18/02/2014, 11:20

... Mason Hawkins, the chairman and CEO of Memphis-based Southeastern Asset Management, spoke the previous day and articulated the opposing view: Margins and earnings are neither at peak or temporarily ... small-cap.” International large- and small-cap stocks and emerging equities are at fair price, he said Grantham said that investors face short-term risks in emerging markets, and that China’s economy ... can be addressed, he said We can have enough energy if we are “sensible” and start building solar- and wind-powered smart electrical grids And if we “recycle like mad,” we will have enough metals...

Ngày tải lên: 06/03/2014, 04:20

... geminin and actin (as loading control) are shown in serum deprived NIH3T3 cells, and and 12 h following serum re-addition, and compared with mRNA levels in proliferating cells Both Cdt1 and geminin ... During the cell cycle, Cdt1 and geminin mRNA levels are mostly stable and protein levels are primary controlled by Fig Expression of Cdt1 and geminin proteins in quiescent and proliferating NIH3T3 ... serum deprivation and contact inhibition, similar to cyclin A and more dramatically than Cdt1 For example, geminin protein levels are undetectable upon serum starvation and are already reduced...

Ngày tải lên: 07/03/2014, 16:20

... thiomidate and acyl-enzyme intermediates, and tetrahedral ( 109.5°) water-bonded intermediate In contrast, serine and thiol protease and amidase are confined to interacting with planar substrates and ... solfataricus The side chains of the putative catalytic residues are shown Ser171 and Lys96, shared by the two triads are in red catalytic triad and forms an angle of about 107° with its plane (Fig 5A) ... catalytic site of the SsAH-WT and SsAH-K96R models are shown in (C) and (D), respectively GLY193 ASP224 Fig Comparison between the putative catalytic sites of the SsAH-WT and SsAH-K96R models with...

Ngày tải lên: 07/03/2014, 21:20

... precursor of the cyanide ligands [6,7] Iron is probably introduced into this process by a complex of HypC and HypD [8] At this complex, the coordination of the CN and CO ligands to iron might be accomplished ... hypB2::Km and DhupE None of the other mutants exhibited hydrogenase activity (Fig 1A) It could thus be concluded that all of the investigated hyp genes, apart from hypA2 and hypB2, are essential ... activity were detected in hypA2 and hypB2 mutants compared with wild-type cells, and neither was able to complement their respective counterparts in the DhypA1 and DhypB1, the transcription of...

Ngày tải lên: 08/03/2014, 08:20

... Healthcare Research and Quality Centers for Disease Control and Prevention Centers for Medicare & Medicaid Services Early and Periodic Screening, Diagnostic, and Treatment Department of Health and ... year, the lower and upper limits of this range are 12.1 million and 13.0 million, respectively 23 U.S Department of Health and Human Services, Agency for Healthcare Research and Quality, “Dental ... dental disease and receive dental care, are not new Our reports dating back to 2000 highlighted the problem of chronic dental disease and the factors that contribute to low use of dental care by lowincome...

Ngày tải lên: 14/03/2014, 09:20

... to and activates b integrins [5] VAV1 and TIAM1 are localized by Rap1GTP to sites of cell spreading and serve as exchange factors for Rac [6] ARAP3 is a GTPase-activating protein for RhoA and ... supernatant (S) and the pellet (P) were analyzed by SDS ⁄ PAGE and quantified by fluorometry Protein precipitation in the absence of liposomes has been substracted Bands below Krit1 band are E coli ... integrin and has been shown to compete with talin for binding to b1 integrin [15] Consistently, on ICAP-1-null osteoblasts and fibroblasts, fibronectin receptors are in an active conformation and b1-dependent...

Ngày tải lên: 16/03/2014, 05:20

... Georgellis and coworkers [22], the quinones are membrane localized electron transporters which are interesting candidates as redox indicators for sensor proteins such as ArcB, BvgS and EvgS As ... standard conditions in the presence of [c-32P]ATP as described above Then mM Q-0 and/ or 16 mM nonradioactive ATP were added After the addition of Q-0 and/ or ATP samples were taken immediately and ... activity of BvgS and EvgS In the genus Bordetella, ubiquinone-8 (Q-8) is the predominant quinone [27], whereas the major quinones synthesized by E coli are Q-8, menaquinone-8 (MK-8) and demethylmenaquinone-8...

Ngày tải lên: 17/03/2014, 23:20

... OldSquarePeg { public: void squarePegOperation() { something } } Adapter Class class PegAdapter: public RoundPeg { private: OldSquarePeg* square; public: PegAdapter() { square = new OldSquarePeg; ... patterns are concerned with how classes and objects are composed to form large structures Different categories Adapter Bridge Composite Decorator Façade Flyweight Proxy Scenario Outlets and Plugs ... SquarePeg class OldSquarePeg { public: void virtual squarePegOperation() { function } } class RoundPeg { public: void virtual roundPegOperation() { function } } class PegAdapter: public OldSquarePeg,...

Ngày tải lên: 19/03/2014, 22:32

... microscopy and CSLM are compared This comparison was B Fig Confocal scanning laser micrographs of microspheres prepared from apo a-LAFITC and unlabelled LYS (A) or unlabelled apo a-LA and LYS-RBITC ... microspheres prepared from apo a-LA-FITC and LYS-RBITC (A), and fluorescence intensity of FITC (green line) and RBITC (red line) along the dotted line (B) Scale bar ¼ lm at the microsphere area The size ... 12 000 g for 30 and the supernatants were recovered and freeze-dried Preparation of LYS ⁄ apo a-LA mixtures Stock solutions of labelled and unlabelled LYS and apo a-LA were prepared by solubilization...

Ngày tải lên: 23/03/2014, 07:20

... complex C1 disappeared and a new complex, SC1, with a higher molecular mass was formed (lane 11), and, when anti-Sp3 IgG was used, bands C2 and C3 disappeared and bands SC3a and SC3b appeared (lane ... DNase II promoter, are essential for both basal and PMA-mediated induction of DNase II transcription These sites bind Sp1 and Sp3, and protein levels and binding of Sp1 and Sp3 are increased in ... ACGCGT and CTCGAG are MluI and XhoI restriction sites, respectively, created to facilitate cloning are underlined b Mutated bases Ó FEBS 2003 Transcriptional regulation of human DNase II by Sp1 and...

Ngày tải lên: 23/03/2014, 17:21

... analysis of gp160 and gp41(34–170) See the Materials and methods for further information (A) Both proteins are glycosylated Proteins were analyzed at pH 2.8, 3.4 and 4.0 in 50 mM formate and adjusted ... prepared and subsequently diluted four-fold with the same solvent (matrix solution) Droplets (1 lL) of a : (v/v) mixture of protein and matrix solution were deposited on the sample slide and ... ultracentrifugation [19] and NMR [9] A major peak corresponding to the monomer, and two smaller peaks corresponding to covalently linked dimeric and trimeric forms of gp41(24–157), are seen in the MALDI-TOF...

Ngày tải lên: 30/03/2014, 13:20

... action and behavioural control (Rutter and Qyne 2002), consumer attitudes and past behaviour (Fishbein et al 2001) Other factors such as the accessibility of medical care and attitudes to health care ... Janz and Becker (1984) and Moorman and Matulich (1993) 27 References Alexander, J.M and B.J Tepper (1995), “Use of reduced-calorie/reduced-fat foods in young adults: influence of gender and restraint”, ... (diet, cigarette smoking) are thought to account for a large proportion of the disparity between life expectancy in the European Union (EU) and the CEE (Bobak and Marmot 1996) There are thus serious...

Ngày tải lên: 08/04/2014, 16:55