peroxynitrite but not no induces apoptosis in human mdmj

... diosgenin-induced human RA FLS death but, as exogenous PGE2 alone did not induce synoviocyte apoptosis, the exact mechanism by which endogenous PGE2 sensitizes human RA FLS to cell death is still not ... with diosgenin-induced human RA FLS death As exogenous prostaglandin E2 (PGE2) alone did not induce synoviocyte apoptosis, the exact mechanism by which endogenous PGE2 sensitizes human RA FLS ... < 0.05) in comparison with diosgenin alone (Fig 8) R379 Arthritis Research & Therapy Vol No Liagre et al Figure distribution and induces apoptosis in the human osteosarcoma 1547 cell line, with...

Ngày tải lên: 09/08/2014, 01:23

... appear necrotic Since cartilage does not have mononuclear phagocytes and is avascular, dead cells or apoptotic bodies are not removed but remain in the lacunae, where they disintegrate and release ... become clear that NO by itself cannot initiate apoptosis and that the concomitant production of superoxide anion is required [10], indicating a role for peroxinitrite in this process In addition, ... PARP inhibitor partially reduced cell mortality, suggesting a role for Fas/CD95 and PARP in HNE-inducing cell death Second, in investigating the classi- cal markers of apoptosis, we obtained...

Ngày tải lên: 09/08/2014, 13:21

... QUIN 350 nM (see Fig legend) Interestingly, our in vitro results on QUIN toxicity in human astrocytes correlate well with previous in vivo studies using animal models After QUIN injection into ... in triplicate throughout Initially, cells were incubated in serum-free AIM-V The negative control cells were incubated in AIMV only The QUIN group was incubated in 350, 500 and 1200 nM QUIN in ... cerebrospinal fluid (CSF), 4',6-diamidino-2-phenylindole (DAPI), Glial fibrillary acid protein (GFAP), human immunodeficiency virus (HIV), kynurenine pathway (KP), NMDA (Nmethyl-D-aspartate), Quinolinic...

Ngày tải lên: 19/06/2014, 22:20

... NS1 protein could induce apoptosis in human airway epithelial cells (NCI-H292) [26] It was also reported that a poor -apoptosis- inducer strain could be converted into a strong-inducer strain Zhang ... death (apoptosis) in human monocytes infected by influenza A virus Immunobiology 1994, 190:175-182 Hinshaw VS, Olsen CW, Dybdahl-Sissoko N, Evans D: Apoptosis: a mechanism of cell killing by influenza ... apoptosis in A549 cells Discussion A number of studies have demonstrated that influenza virus infection can induce apoptosis in a variety of cell lines, but the mechanism of this effect remains...

Ngày tải lên: 12/08/2014, 04:20

... with vinblastine in inhibiting MCF-7 cell proliferation Curcumin affected the localization of the kinesin protein Eg5 Because curcumin produced monopolar spindles in MCF-7 cells, we examined the ... rate (lmÆmin ) Growth length (lm) Growth time (min) Shortening rate (lmÆmin)1) Shortening length (lm) Shortening time (min) Pause time (min) % Time spent in growing % Time spent in shortening % Time ... incubated either separately with curcumin, paclitaxel and vinblastine or in combination with curcumin and vinblastine or paclitaxel for one cell cycle The combination index was calculated using...

Ngày tải lên: 23/03/2014, 03:20

... The intracellular Cu level was also increased by Py/Zn treatment Although Py treatment alone, but not Zn treatment, enhanced intracellular Cu levels, Py did not induce apoptosis, indicating that ... depending on the intracellular Zn levels Induction of apoptosis by the elevation of intracellular Zn levels at a low concentration but not a high concentration Next, we investigated the involvement ... aspects in the regulation of apoptosis is the involvement of mitogen-activated protein kinases (MAPKs), a family of serine/threonine kinases that mediate intracellular signal transduction in response...

Ngày tải lên: 23/03/2014, 21:20

... concentrations could be beneficial in chemotherapy Similar to taxol and vinblastine, benomyl also induces apoptosis in HeLa cells, indicating that benomyl may be useful in combination with other potent ... distinct from the colchicine and vinbastine binding sites in tubulin [10,11] Although benomyl inhibits mitosis in mammalian cells, its antimitotic mechanism of action is not clearly understood ... those that are not aligned at the metaphase plate (Fig 5) The results indicated that the sister kinetochores are not under tension in the presence of benomyl, resulting in the inhibition of degradation...

Ngày tải lên: 30/03/2014, 10:20

... carcinoma, NKC : non-keratinizing carcinoma, UC : undifferentiated carcinoma d Clinical staging: primary tumor extension classified T2, T3 or T4 according to AJCC/UICC (1997); regional lymph node ... LMP1 staining visible in malignant cells, from one patient to another and within a given tumor We found no NPC specimens with complete absence of S12 staining Even when staining was minimal, ... moderate intensity, thus resulting in a score of In contrast, we found a complete absence of staining on sections of lung or laryngeal carcinomas used as negative controls, resulting in a minimal...

Ngày tải lên: 19/06/2014, 08:20

... carcinoma, NKC : non-keratinizing carcinoma, UC : undifferentiated carcinoma d Clinical staging: primary tumor extension classified T2, T3 or T4 according to AJCC/UICC (1997); regional lymph node ... LMP1 staining visible in malignant cells, from one patient to another and within a given tumor We found no NPC specimens with complete absence of S12 staining Even when staining was minimal, ... moderate intensity, thus resulting in a score of In contrast, we found a complete absence of staining on sections of lung or laryngeal carcinomas used as negative controls, resulting in a minimal...

Ngày tải lên: 20/06/2014, 04:20

... and initiation of cART were not associated with shorter times to death in either model These associations were not appreciably altered in models that did not adjust for cART initiation (data not ... initiation as a time-dependent variable We chose not to include weight in these initial models as previous studies suggested that the inclusion of weight in the Cockcroft-Gault formula, but not ... proteinuria, but not inverse creatinine, was significantly associated with the development of a new AIDS-defining illness Several reasons may explain the differences in results The WIHS cohort included...

Ngày tải lên: 20/06/2014, 08:20

... cocaine on fetal brain apoptosis Assessment of nuclear chromatin morphology by Hoechst 33258 staining indicated that cocaine increased condensed and segmented apoptotic nuclei in the fetal brain ... defined by the fluorescent DNA-binding dye Hoechst 33258 indicated that cocaine produced a dose-dependent increase in apoptosis in the fetal brain (Fig 1, the lower panel) Effects of cocaine ... fetal brain exposure to cocaine is somewhat prolonged Our current finding that cocaine had no effect on the activities of the caspases in the maternal brain but only in the fetal brain, further...

Ngày tải lên: 08/08/2014, 17:20

... and in vivo studies showed that methotrexate is ineffective in the presence of antagonists of adenosine or adenosine deaminase (the enzyme responsible for the deamination of adenosine to inosine) ... a known anti-inflammatory cytokine, in wild-type mice and A3 knockout mice, but actually decreased IL-10 levels in A2A knockout mice We not have a clear explanation for this other than to note ... ribonucleotide, which is an intermediate metabolite in the denovo pathway of purine synthesis, and has been associated with increases in extracellular adenosine [9,13,53] There is now increasing evidence that...

Ngày tải lên: 09/08/2014, 07:20

... phosphoinositide 3-kinase/Akt pathway is being increasingly recognized as playing a major role in synovial fibroblasts It is used in response to TRAIL (TNF-related apop- Page of 11 (page number not ... simvastatin from that of atorvastatin was observed in both activated and non-activated RA synovial fibroblasts but not OA synovial fibroblasts, suggesting a fundamental difference in their intracellular ... prepared from RA synovial fibroblasts grown in increasing concentrations of lovastatin contained increasing amounts of histone bound DNA fragments, suggesting the induction of apoptosis (Figure...

Ngày tải lên: 09/08/2014, 08:22

... of CVD [57,58] In RA antiPC have not been studied in relation to CVD, but we have recently shown that the levels of anti-PC in serum increased in RA patients when changing from a normal Western ... with an increased risk of development of CVD [11] In RA, anti-PC have not been studied in relation to CVD but we have recently shown that the level of anti-PC in serum increased when changing from ... value less than 80 cm in women and 94 cm in men indicates low risk, 80–87.9 cm in women and 94–101.9 cm in men indicates intermediate risk, and a value Page of 11 (page number not for citation purposes)...

Ngày tải lên: 09/08/2014, 13:22

... all-trans being the predominant physiological form Endogenous retinoids with biological activity include alltrans retinoic acid, 9-cis retinoic acid, 11-cis retinaldehyde, 3,4-didehydro retinoic acid ... al.: All-trans retinoic acid inhibits KIT activity and induces apoptosis in gastrointestinal stromal tumor GIST-T1 cell line by affecting on the expression of survivin and Bax protein Journal of ... mechanisms of retinoic acids on regulating differentiation, proliferation and apoptosis are not fully elucidated, it has been suggested that induction of differentiation and apoptosis by retinoic acids...

Ngày tải lên: 10/08/2014, 10:20

... researches have indicated that the apoptosis- inducing properties of ATO are not restricted to APL, since the viability of different cancer cell lines that originate from the same lymphoid lineage vary ... is involved in ATO-induced apoptosis in HL-60 cells However, further investigations are needed to determine whether or not specific activators of caspace3 may be directly associated with the induction ... extrinsic death receptor pathway mediated by Fas and caspase-8 activation in acute megakaryocytic leukemia [47] Our result is in support of previous findings indicating that ATO induces clinical...

Ngày tải lên: 10/08/2014, 22:21

... did not affect TSA-induced apoptosis in human eosinophils (n = 6, data not shown) Involvement of caspases in TSA-induced apoptosis in human eosinophils Even though the involvement of caspases in ... cytokines IL-5 and GM-CSF induce eosinophil survival or glucocorticoids induce eosinophil death are not known in detail [3,22,30] In fact, it is not even known whether glucocorticoid-induced apoptosis ... NF-B p65 does not explain the apoptosisinducing effect of TSA in human eosinophils The above data suggest that the effects of HDAC inhibitors in eosinophils or neutrophils may not be mediated...

Ngày tải lên: 11/08/2014, 08:22

... Nuclear staining with DAPI was used to determine apoptosis- inducing activity of TXL in HT-29 cells After TXL (1%) treatment, HT-29 cells underwent typical morphologic changes of apoptosis including ... Wang WB: The chinese herbal medicine Tien-Hsien liquid inhibits cell growth and induces apoptosis in a wide variety of human cancer cells J Altern Complement Med 2005, 11:245-256 Ninomiya I, Terada ... molecular events in TXLtreated HT-29 cells result in remarkable apoptosis process Further in vivo and in vitro studies are needed to clarify the protein interactions, thereby delineating the upstream...

Ngày tải lên: 13/08/2014, 15:21

... calnexin-containing but not in calnexin-deficient cells HPV16 E5 decreases HLA-I surface expression in calnexin-containing but not in calnexin-deficient cells CEM-C7 (calnexin) and CEM-NKR (no calnexin) ... with pCI-E5-HA were stained with anti-HLA-A, B, C mAbs B9.12 E5 expression results in diminished HLA-I surface staining in cells expressing calnexin, but not in calnexin deficient cells D) The ... reduces cell surface expression of HLA-I molecules in calnexin-expressing but not in calnexin-deficient cells Since calnexin plays an important role in maturation of the HLA-I complex, we decided...

Ngày tải lên: 18/06/2014, 18:20

... brief, human embryonic brain tissues were dissected into small blocks, incubated in phosphate-buffered saline (PBS) containing 0.25% trypsin and 40 µg/ml DNase and then dissociated into single ... P2YR and ionotropic P2XR) but not BzATP (activator of the ionotropic subtype P2X7R), was found to induce production of the cytokine The role of SOC in MG-5 cell responses is unclear since ATP evokes ... an inducer of IL-6 expression and production in MG-5 microglial cell line [12] ATP and the purinergic agonist BzATP were both effective in increasing expression of IL-6 with effects involving...

Ngày tải lên: 19/06/2014, 22:20