peripheral blood mononuclear cells pbmc

Ngày tải lên: 11/09/2015, 09:18

... pyrogenic cytokines from peripheral blood mononuclear cells (PBMC) and the pyrogenic response in rabbits Results Febrile response to the supernatant fluids of the SEC1-stimulated PBMC To determine whether ... Staphylococcal enterotoxin A acts through nitric oxide synthase mechanisms in human peripheral blood mononuclear cells to stimulate synthesis of pyrogenic cytokines Infect Immun 68, 2003–2008 60 ... nonheated SEC1 group) or antibody (compared with the SEC1-treated PBMC plus IgG group) SEC1 induces NF-jB activation in PBMC PBMC were treated in the presence or absence of NFjB inhibitor pyrrolidine...

Ngày tải lên: 19/02/2014, 00:20

... Effects of SEB on human PBMCs C Mendis et al Proliferation assays Human PBMCs were isolated and purified as described previously [31], except that human PBMCs were used at 2.5 · 106 cells mL)1 in RPMI ... 2008 FEBS C Mendis et al Effects of SEB on human PBMCs Table Effect of various inhibitors on SEB-induced signaling pathways Human PBMCs (2.5 · 106 cells mL)1) were treated with the respective inhibitor ... Fig Concentration dependence of SEB on PBMC proliferation A typical SEB-induced time-dependent cell proliferation pattern is shown Human PBMCs at 2.5 · 106 cells mL)1 density were incubated in...

Ngày tải lên: 30/03/2014, 04:20

... capable of reaching the immune system and peripheral blood stream Therefore, we hypothesized that the percent of peripheral blood mononuclear cells (PBMCs) displaying p-STAT-3 may be increased ... immune cells in the cervical lymph nodes, which then are detected in the peripheral circulation Therefore, we measured p-STAT-3 in glioma patients' peripheral blood mononuclear cells (PBMCs) ... been shown to inhibit Tregs [26,36] Materials and methods Acquisition of peripheral blood and tumor specimens Peripheral blood samples (N = 45) were collected from patients prospectively, usually...

Ngày tải lên: 18/06/2014, 15:20

... myeloid-derived suppressor cells; (NOX2): NADPH oxidase; (NFκB): nuclear factor kappa B; (PBMC) : peripheral blood mononuclear cells; (PGE): prostaglandin E2; (Treg): Page 19 of 20 regulatory T cells; (STAT3): ... method to induce human MDSC from healthy donor peripheral blood mononuclear cells (PBMC) by co-culture with human solid tumor cell lines Suppressor cells generated by this method demonstrate features ... Epstein AL: Characterization of cytokine-induced myeloid-derived suppressor cells from normal human peripheral blood mononuclear cells J Immunol 2010, 185:2273-2284 Kusmartsev S, Gabrilovich DI: Effect...

Ngày tải lên: 18/06/2014, 19:20

... Indian ALS patients [9] We now measured the mRNA expression of VEGF-A and CCL2 in peripheral blood mononuclear cells (PBMCs) of these patients Subjects and methods 50 patients, born in North India ... sclerosis; VEGF-A, vascular endothelial growth factor-A; CCL2, chemokine ligand-2; PBMCs, peripheral blood mononuclear cells levels did not vary between definite, probable and possible ALS cases (Figure ... sclerosis; VEGF-A, vascular endothelial growth factor-A; CCL2, chemokine ligand-2; PBMCs, peripheral blood mononuclear cells and differentiation of VEGF receptor (VEGFR1), VEGFR2 and chemokine receptor...

Ngày tải lên: 19/06/2014, 22:20

... to most users Reproducibility has been achieved Table Gene expression studies of peripheral blood mononuclear cells from patients with autoimmune diseases Subjects studied Gene arrays used Summary ... Therapy 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 128 Vol No Olsen et al in peripheral blood mononuclear cells from lupus patients by cDNA array Clin Immunol 2002, 102:283-290 Baechler EC, ... J, Aune TM: A Gene Expression Signature for Recent Onset Rheumatoid Arthritis in Peripheral Blood Mononuclear Cells Ann Rheum Dis 2004, in press Maas KH, Westfall M, Pietenpol J, Olsen N, Aune...

Ngày tải lên: 09/08/2014, 01:23

... tissues, profiling peripheral blood cells has emerged as an attractive biomarker discovery strategy [10,16-22] Another added advantage to analyzing peripheral blood cells is the fact that blood is a ... present study, we profiled gene expression in rat peripheral blood mononuclear cells (PBMCs) during the development of CIA We established the method for blood collection, Page of 14 (page number not ... profiling in peripheral blood mononuclear cells in the collagen-induced arthritis model To identify putative biomarkers for arthritis, we surveyed global gene expression profiles of PBMCs in a...

Ngày tải lên: 09/08/2014, 07:20

... systemic lupus erythematosus sera can induce interleukin-10 production from normal peripheral blood mononuclear cells (PBMC) via FcγRIIa [17] Based on the hypothesis that RF production in RA mirrors ... and the University Hospital in Uppsala Preparation of peripheral blood mononuclear cells and cell cultures Buffy coats obtained from healthy blood donors were diluted in PBS at room temperature ... immunosorbent assay; PBMC, peripheral blood mononuclear cell; RA, rheumatoid arthritis; SF, synovial fluid; TNF, tumour necrosis factor phages [9] and monocyte-derived dendritic cells [10] in ICinduced...

Ngày tải lên: 09/08/2014, 07:20

... disease; HLA, human leukocyte antigen; HO, heme oxygenase; PBMC, peripheral blood mononuclear cells; PMN, peripheral blood multinuclear cells Page of 10 (page number not for citation purposes) ... Figure TLR2 and TLR4 mRNA expression in PBMCs and PMNs from patients with BD Expression levels in peripheral blood mononuclear cells (PBMCs) of expression in PBMCs and PMNs from patients with BD ... individual cells from patients with active disease As shown in Figure 1b, HO-1 mRNA lev- Figure HO-1 mRNA expression in PBMCs and PMNs from patients with BD (a) Peripheral blood mononuclear cell (PBMC) ...

Ngày tải lên: 09/08/2014, 10:22

... expression was calculated using the 2-ΔΔCt method [23] In this study, we obtained peripheral blood mononuclear cells (PBMCs) from RA patients and control individuals and examined the expression of ... M-CSF, macrophage colony-stimulating factor; PBMC, peripheral blood mononuclear cell; SEM, standard error of the mean; TNF, tumor necrosis factor RA patient PBMCs exhibit increased expression of miR146a, ... determined using Image J analysis software SEM is shown; n > 20 cells IRAK, IL-1 receptor-associated kinase; PBMC, peripheral blood mononuclear cell; qRT-PCR, quantitative real-time RT-PCR; RA,...

Ngày tải lên: 09/08/2014, 13:21

... cytometry sorting of peripheral blood mononuclear cells (PBMC) subpopulations: CD3/CD19, CD14, and CD16 A-D Flow cytometry sorting of peripheral blood mononuclear cells (PBMC) subpopulations: ... remain to accurately characterize these cells Firstly, tumor cells in circulation are infrequent, ranging from 1/105 to 1/107 peripheral blood mononuclear cells (PBMC) , even in patients with metastatic ... F End cells C D E F Ber EP4 Antibody used for selection Figure normal subjects CK19 and HER2 RNA expression from serial immunomagnetic selection of peripheral blood mononuclear cells (PBMC) from...

Ngày tải lên: 10/08/2014, 22:20

... CD4+ T cells in CD4+ T cells Day0 IL-17+CD4+ Fold increase in the percentage of CD4+ IL-17+ T cells Page of 11 Figure Increased percentages of IL-17+ CD4+ T cells and IL-17+ CD8+ T cells in PBMCs ... culture of PBMCs Seventeen milliliters of venous peripheral blood from patients and normal subjects was collected in ACD blood collection tubes (BD Pharmingen, San Diego, CA, USA) PBMCs were ... %IL-17A+CD8+ T cells Day3 %IL-17A+CD4+ T cells Day3 (c) 20 + %IL-23R CD4 T cells Day3 10 40 r = 0.4833 p*= 0.0079 30 20 10 0 10 20 30 + + 40 50 %IL-23R CD4 T cells Day3 %IL-23R CD8 T cells Day3 Total...

Ngày tải lên: 12/08/2014, 15:22

... methylated bovine serum albumin; MCs: mast cells; PM/DM: polymyositis/dermatomyositis; PBMCs: peripheral blood mononuclear cells; PBMCs: peripheral blood mononuclear cells; RasGRP: Ras guanine nucleotide ... expression in peripheral blood A, Evaluation of hRasGRP4 mRNA levels in unfractionated peripheral blood mononuclear cells (PBMCs), and PBMC- derived CD14+ cells, CD14-/CD3-/CD19- cells, and T cells isolated ... unidentified population of peripheral blood mononuclear cells (PBMCs) express Ras guanine nucleotide releasing protein (RasGRP4) The aim of our study was to identify the cells in human blood that express...

Ngày tải lên: 12/08/2014, 18:20

... identified as HTLV-1-immortalized PBMCs cultured alone (PBMC- 1, PBMC- 2) or the in the presence of IL-2 (PBMC1 + IL-2, PBMC- 2 + IL-2) or phytohemagglutinin (PHA) (PBMC- 1+PHA, PBMC- 2 + PHA) without HTLV-1 ... in PBMCs at weeks 1, 3, 5, 7, 9, 11, and 13 following HTLV-1 infection in the presence or absence of IL-2 compared to day 0; controls and are PBMC- 1 and PBMC- 2; controls and are PBMC- 1 and PBMC- 2 ... IL-2 (hIL-2) (PBMC- 1, 2, + HTLV-1+ IL-2; PBMC- 1, 2, represent PBMCs from three different donors) Viable cells were counted weekly by trypan blue exclusion Cells that continued to produce p19 Gag...

Ngày tải lên: 13/08/2014, 05:20

... http://www.retrovirology.com/content/5/1/24 Figure Composite dot scan patterns of antibody binding for PBMC cells Composite dot scan patterns of antibody binding for PBMC cells Half of the duplicate array was shown with the alignment ... making microarrays at concentrations ranging from 50–1000 μg protein/ml Immunophenotyping of PBMC Mononuclear cells were purified by Ficoll density gradient centrifugation and cryopreserved in fetal ... NK-associated marker and its expression on CD8+ T cells identifies the mature cytolytic effector cells [37,38] CD57 expression on CD8+, CD4+ T cell and NK cells is a general marker of cell proliferative...

Ngày tải lên: 13/08/2014, 06:20

... Supernatants from transfected cells were assayed for HIV-1 p24 antigen (Beckman Coulter, Miami, FL) and infectivity [28] PBMC Infections Human peripheral blood mononuclear cells (PBMC) were collected, ... 40 tRNAPro2 human peripheral blood mononuclear cells complementary to the 3' 18 nucleotides of tRNAIle, to be Replication of HIV-1 with PBS sequence altered tRNAMet and Figure (PBMC) in Replication ... complementary to the 3' 18 nucleotides of tRNAIle, tRNAMet and tRNAPro in human peripheral blood mononuclear cells (PBMC) Infections were initiated with transfection supernatant containing approximately...

Ngày tải lên: 13/08/2014, 09:21

... septic shock, the hemodynamic response to β1-AR agonists was impaired, and in peripheral blood mononuclear cells (PBMCs), a decreased β2-AR-mediated cAMP production was found [11-13] In septic ... conditions PBMCs were isolated with the use of a Ficoll density gradient (Biochrom, Berlin, Germany) within 30 minutes after collecting peripheral venous blood from volunteers or septic patients Cells ... long been recognized that β-adrenergic stimulation suppresses LPS-induced TNF-α release from blood cells in vitro and in vivo [6] and thus could ameliorate the inflammatory response In severe...

Ngày tải lên: 13/08/2014, 11:23

... from peripheral blood and bone marrow (Table 2) On/off-phenomena of the identified spots were observed in four cases: Spot No 19 (annexin 6) was found in patient A in the sample of peripheral blood ... bone marrow aspirates in comparison to blasts from peripheral blood samples not differ basically This may indicate, that samples of peripheral blood with high amounts of blasts are to be considered ... have shown a strong correlation of surface antigen expression comparing bone marrow and peripheral blood blast cells [11] However, it remains unclear, whether there are differences in expression...

Ngày tải lên: 18/06/2014, 15:20