perilipin and hormone sensitive lipase

... of hormone- sensitive lipase Egan JJ, Greenberg AS, Chang MK, Wek SA, Moos MC Jr & Londos C (1992) Mechanism of hormonestimulated lipolysis in adipocytes: translocation of hormone- sensitive lipase ... behavior of hormone- sensitive lipase reflect the absence of the lid domain? Biochemistry 43, 9298–9306 15 Holm C, Fredrikson G, Sundler R & Belfrage P (1990) Incorporation of hormone- sensitive lipase ... italic) and pVL1393–HSL [18] as template was digested using XbaI and BssHII and subcloned into pVL1393–HSL PCR was performed using Vent polymerase (New England Biolabs, Ipswich, MA, USA), and the...

Ngày tải lên: 18/02/2014, 11:20

... physiological structures On the other hand, it has been proposed that the hh dimer and tt dimer structures both occur, and represent the inactive and the hormone- activated states of the receptor, ... subdomain (Fig 1B) and a tt dimer associated with the membrane-proximal subdomain (Fig 1C) The buried surface areas in the hh and tt ˚ contacts in crystals are estimated to be 1100 A2 and ˚ 2, respectively ... [15–17] and the erythropoietin receptor [18,19] In contrast, our proposed rotation mechanism, which is based on the hh dimer structures for both apoECD and ANP–ECD, is mediated by a ligand-induced...

Ngày tải lên: 07/03/2014, 03:20

... between nouns and their adjectival forms The function (%sim arg0 CAT) reflects the perceived similarity between the putative member arg0 and a synset CAT in WordNet, using one of the standard formulations ... %sim in the functions of Figures 2, and means that these membership functions readily admit both literal and metaphoric members Since the line between literal and metaphoric uses of a category ... rich enough, and individually salient enough, to predict how each category is perceived and applied by a language user If similes are indeed a good basis for mining the most salient and diagnostic...

Ngày tải lên: 08/03/2014, 02:21

... phospholipase A2 from spleen, lung and kidney: antiserum raised to pig spleen phospholipase A2 recognizes a similar form in bovine lung, kidney and platelets, and immunoprecipitates phospholipase ... purified enzyme resulted in the release of AA Km and Vmax values obtained for the reactions were 4.1 and lM and 948 and 193 nmolÆmin)1Æmg)1 for CTE-I and a MTE-I, respectively Next, we measured the ... both recombinant proteins (Fig 1A, lanes and 5) and the 43-kDa protein from mitochondrial and postmitochondrial fractions of Y1 tumor cells (Fig 1A, lanes and 7) Preadsorbed antibody with the purified...

Ngày tải lên: 08/03/2014, 09:20

... tagging and context sensitive lemmatization was introduced The morphological analyzer is based on the standard Duden grammar and provides wide coverage due to a lexicon o f 324,000 word forms and ... possible prefixes and suffixes and reverses the process of vowel mutation if umlauts are found (shifts between B and ss are treated analogously) Each root is looked up in the lexicon, and - if found ... dust corners); and the linking letters e and s are not always determined phonologically, but in some cases simply occur by convention (e.g Schwein-e-bauch but Schwein-s-blase and Schwein-kram)...

Ngày tải lên: 17/03/2014, 07:20

... electronic components and measure characteristics Fig.5 is a photo of experimental amplifier The first and second stages have output power of 45 W and 90 W, corresponding, of a standard input 300 ... linearity and large dynamic band enables the amplifier of ECC-01 can receive UHF signals from -30dBm to -108dBm Furthermore, a Timing Automatic Gain Controller (TAGC) is used to enhance dynamic band ... unwieldy and needed so many logic gates with uncontrolled of delay time and bits properties In our method, the microcontroller PIC16F877A has been selected The circuit is very simple and with...

Ngày tải lên: 22/03/2014, 11:20

... phenanthroline/Fe2+ complex, and then quantifying the concentration of a known dilution spectrophotometrically [14] Cell lines and flow cytometry MCF7, BT-474, and MDA-MB-231 breast cancer cells and Chinese hamster ... streptomycin (vol/vol), and μg/mL ciprofloxacin MCF7/Her2-18 and parental CHO cells were cultured in an incubator at 37°C with 5% CO2 and maintained at a cell concentration of between × 105 and × 10 viable ... National Institutes of Health Guide for the Care and Use of Laboratory Animals and approved by the Institutional Animal Care and Use Committee Results and discussion Characterization of nanoparticles...

Ngày tải lên: 22/03/2014, 17:20

... Radiographic and CT Findings Reviews of chest radiographs and CT scans were performed at least two months apart for concern of recall bias Chest radiographs and CT scans were reviewed in a random order ... not significant Numbers represent mean standard deviation of involved zones Numbers in parentheses are median, standard error and range DS TB = drug -sensitive tuberculosis, MDR TB = multidrug-resistant ... not significant Numbers represent mean standard deviation of involved lobes Numbers in parentheses are median, standard error and range DS TB = drug -sensitive tuberculosis, MDR TB = multidrug-resistant...

Ngày tải lên: 22/03/2014, 18:20

... lipid membranes and prostaglandins Microbial lipases are used to obtain PUFAs from animal and plant lipids such as menhaden oil, tuna oil, and borage oil Free PUFAs and their mono- and diglycerides ... types of lipases, Lipases I and II, are known to be produced by Rhizop niveus (Kohno et al., 1994) Lipases I and II differ in molecular weight and Lipase I appears to be converted to Lipase II by ... Purification and kinetic characterization of lipases Many lipases have been extensively purified and characterized in terms of their activity and stability profiles relative to pH, temperature, and effects...

Ngày tải lên: 24/03/2014, 05:20

... antenna and connected chip http://www.instructables.com/id/RFID_Reader_Detector _and_ Tilt _Sensitive_ RFID_Tag/ step 1: Material and Tools This section provides an overview of the necessary materials and ... antenna, and therefore also variable reading distance of the tag (Figure 1) - Experiments with the tag size and material (Figure 2) - Switching between the LED and an RFID chip (Figure 3) - Light -sensitive ... http://www.instructables.com/id/RFID_Reader_Detector _and_ Tilt _Sensitive_ RFID_Tag/ Image Notes Button to switch between LED reader detection and the RFID tag RFID chip MIFARE 1k Image Notes Light sensitive tag Image Notes Capacitive touch-sensitive...

Ngày tải lên: 18/04/2014, 09:11

... documented before therapy and months after starting the hormone treatment The statistical analyses were performed with the commercial statistical package SAS 10.0 Results and comments Altogether, ... physician) 70.8% and spe- Page of (page number not for citation purposes) Health and Quality of Life Outcomes 2006, 4:32 http://www.hqlo.com/content/4/1/32 Table 3: Values for Sensitivity and Specificity ... design of this validation analysis, and involved in writing the manuscript; DS: responsible for setting up and managing the database for different analyses, and for running all analyses We hope...

Ngày tải lên: 20/06/2014, 15:20

... Gonadotropin-Releasing Hormone and Gonadotropic Hormones The pituitary hormones, luteinizing hormone (LH) and follicle-stimulating hormone (FSH), as well as the related placental hormone chorionic ... hypothalamic releasing hormones include growth hormone releasing hormone (GHRH), gonadotropin-releasing hormone (GnRH), thyrotropin-releasing hormone (TRH), and corticotropin-releasing hormone (CRH) ... gonadotropin-releasing hormone (GnRH), controls the synthesis and release of both gonadotropins (LH and FSH) in males and females Gonadal steroid hormones (androgens, estrogens, and progesterone)...

Ngày tải lên: 12/07/2014, 20:21

... AL) and used as purchased The restriction enzymes EcoRI and EcoRV were purchased from New England Biolabs (Ipswich, MA) Custom ssDNA sequences designated C1 (5’amino-ACGTACGTGATATCTGCATGCA-3’) and ... details Department of Biomedical Engineering, Vanderbilt University; Nashville, Tennessee, USA 2Vanderbilt Institute for Nanoscale Science and Engineering, Vanderbilt University; Nashville, Tennessee, ... Vanderbilt University; Nashville, Tennessee, USA Integrative Biosciences Institute, École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland Authors’ contributions SSY and RLS planned and...

Ngày tải lên: 11/08/2014, 00:23

... Hubbell4 and Todd D Giorgio1,2* Affiliations: Department of Biomedical Engineering, Vanderbilt University; Nashville, Tennessee, USA Vanderbilt Institute for Nanoscale Science and Engineering, Vanderbilt ... Vanderbilt University; Nashville, Tennessee, USA Integrative Biosciences Institute, École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland Email: SSY : shann.yu@vanderbilt.edu RLS: randy.l.scherer@vanderbilt.edu ... randy.l.scherer@vanderbilt.edu RAO: ryan.a.ortega@vanderbilt.edu CSB: charleson.bell@vanderbilt.edu CPO'N: conlin.oneil@epfl.ch JAH: jeffrey.hubbell@epfl.ch TDG: todd.d.giorgio@vanderbilt.edu *Corresponding...

Ngày tải lên: 11/08/2014, 08:20

... metabolism and hormone metabolism and signaling using cDNA derived from leaf and fruit cortex (FC) (Figures and 9) In both FAZ and FC, the expression of MdNCED was induced by both shading and NAA ... photosynthesis impairment and causes altered hormone signaling and triggers fruit abscission Results Effects of NAA and shading on young fruit abscission and ethylene production by young fruit and leaves A ... 25 and 26 d after treatment For ethylene production measurements, 15 fruit and 20 leaves were collected from each of three replicate trees and h and 1, 3, 5, 7, 9, 11 and 14 d after treatment and...

Ngày tải lên: 11/08/2014, 11:21

... exhibited modulation of expression in response to two hormones (Table 2) At h, both JA and ET repressed BnWRKY11 and both ET and BAP repressed BnWRKY1, 20 and3 2 (Figure 4C, Table 2) However, none of the ... genes to hormone treatments To investigate the hormonal control mechanisms underlying BnWRKY gene expression, we treated canola plants with five phytohormones, JA, SA, ABA, BAP and ET and analyzed ... for JA [50]; ethylene insensitive (EIN2) [89] and ethylene responsive factor (ERF2 and ERF4) [90] for ET; ABA insensitive (ABI5) [91-93] for ABA, and Arabidopsis http://www.biomedcentral.com/1471-2229/9/68...

Ngày tải lên: 12/08/2014, 03:20

... Hetero-Chromatization And Fragmentation Cytoplasmic Swelling Mitochondrial Dysfunction Membrane Rupturing Membrane Blebbing And Apoptotic Bodies Random and Violent Death Cell Suicide And Dismantling ... activation of NF-κB and the signal transducer and activator of transcription, and that increases in IL-6 and granulocyte colony-stimulating factor mRNA levels in the lungs and livers measured ... financially supported by the Anonymous Trust (Scotland), the National Institute for Biological Standards and Control (England), the Tenovus Trust (Scotland), the UK Medical Research Council (MRC, London),...

Ngày tải lên: 12/08/2014, 19:21

... regulatory element in sensing hypoxic conditions and in integrating an adapted response via gene expression of oxygen -sensitive and redox -sensitive enzymes and cofactors is hypoxiainducible factor-1 ... that of NF-κB Indirect, but unprecedented and unequivocal, evidence was independently provided by Hellwig-Bürgel and colleagues [47–49] and by Haddad and Land [50,51], however, to indicate HIF-1 ... financially supported by the Anonymous Trust (Scotland), the National Institute for Biological Standards and Control (England), the Tenovus Trust (Scotland), the UK Medical Research Council (MRC, London),...

Ngày tải lên: 12/08/2014, 19:21

... pseudotyped Lv 2sensitive and Lv2-insensitive HIV-2 viruses are restricted by human TRIM5α HIV-2[VSV-G] and HIV-2/I73V[VSV-G] viruses were used to infect TZM-LNCX2 cells (closed symbols) and TZM-huTRIM5α ... between Hela-cells and TZM-bl cells could account for the observed stronger restriction and need to be further characterized The challenge experiments using Lv2 -sensitive and Lv2-insensitive HIV-2 ... from PD Bieniasz, [21]) and G418 resistant cells were selected TZM-bl cells are HeLa-cell derivatives that express high levels of CD4 and both co-receptors CXCR4 and CCR5, and are stably transduced...

Ngày tải lên: 13/08/2014, 09:20

... Menopause, Hormone Therapy, and Women’s Health Box 243—The Production of Estrogen and Progesterone in the Reproductive Cycle Hormones are chemical messengers that are produced by specialized glands and ... The Menopause, Hormone Therapy, and Women’s Health Table 2-l—Rates of Hysterectomies (per thousand) by Age and Geographic Region, United States, 1972-87 Age and region 1972 15 and older United ... Total and Free Estrogens and Sex Hormone- Binding Globulin With Lipoproteins in Women,” Journal of Clinical Endocrinology and Metabolism 71(1):67-72, 1990 30 The iWenopause, Hormone Therapy, and...

Ngày tải lên: 12/02/2014, 23:20