peptides proteins or subcellular organelles single molecule studies and nanomedical approach

... these important proteins at the singlemolecule level Single- molecule manipulation using magnetic tweezers and single- molecule imaging using atomic force microscopy were utilized in the works leading ... Wenwen, and many others Importantly, I want to thank my parents, family, and friends, who have supported me My fianc`e Marissa Iskandar for her continuous support e and prayer Their presence, support, ... Cynthia He, for their advices and direction for this thesis iii Acknowledgements iv My colleagues in single- molecule biophysics laboratory: Lim Ci Ji for the stimulating discussions, advices, and constructive...

Ngày tải lên: 09/09/2015, 11:26

... Conductance of Single Nanotubes and Nanowires 7.3.4 DNA Molecules 7.3.5 Single Molecule Devices Single Molecule Fluorescence Imaging and Spectroscopy: ... internal molecular structures and environmental factors, and has Introduction to Single Molecule Chemistry and Physics become a test ground for many experimental and theoretical methods Advances ... widely used approach is fluorescence detection, used for single molecule work at both cryogenic and ambient temperatures Last but not least, the enhancement of Raman signals at single molecule level...

Ngày tải lên: 27/06/2014, 10:20

... Pioneering work on combined optical trap and singlefluorophore experiments has come from the laboratory of Yanagida [29,30] In 1998 Ishijima et al [29] reported simultaneous monitoring of both force and ... existing single- molecule techniques, in particular those that measure optical and mechanical signals Single- molecule fluorescence Single- fluorophore detection entails the illumination of a molecule ... behavior of individual molecules inside living cells [16-18] Single- molecule forces Two principal methods are available for resolving the forces exerted by single molecules [19], optical traps (or...

Ngày tải lên: 06/08/2014, 18:20

... limited and they are used in all forms of living organisms In later chapters, organization and compaction of DNA by kinds of proteins are studied using Atomic Force Microscope (AFM) and transverse ... experience for doing research and study in Biophysics & Single- molecule manipulation Lab I am grateful to Dr Chen Hu, Dr Fu Hongxia, Dr Fu Wenbo, Law Dingying and all my group members for their help and ... important in genomic compaction and function for all organisms My Ph.D research focuses mainly on the understanding of how these proteins perform their functions and on the study of DNA-protein...

Ngày tải lên: 14/09/2015, 08:39

... bias and error For example, the effect of a chemical substance on germination and rooting cannot be evaluated because the green alga is a unicellular organism Furthermore, different toxicity for ... Chronic Tests of Alga, Daphnid and Fish 17 OECD Environmental Performance Reviews: Japan (2002) Organisation for Economic CoOperation and Development, Organization for Economic (2002/05) OECD TG ... at cellular and subcellular levels Organelles in the cytoplasm become compacted together, cytoplasm viscosity increases and a number of degenerative reactions intensify In order for the cell...

Ngày tải lên: 22/06/2014, 03:20

... Impact Studies and Management Approaches Effect of landscape position on phytotoxicity and dissipation of flucarbazone and sulfentrazone Farm fields with irregular rolling topography of low hills and ... herbicide Samples for the tests were taken four or five times per day for the first days, once a day for the next 10 days, and then once every few days The observation was performed for one year The ... Environmental Impact Studies and Management Approaches of monitoring The toxicity of sample 2, treated with NMU for h, remained high (~90%) for 36 days Then, it decreased abruptly, and, after 56 days,...

Ngày tải lên: 22/06/2014, 03:20

... chloride IV 3,6-Dichloropicolinic acid V 4-Chlorobenzoyl chloride VI Trichlorobutanol VII 2,3,5-Trichloropyridine VIII Trichlorobutanol (isomer) IX Hexachlorocyclohexane X 2,6-Dichloro-3-nitropyridine ... of ΔF/Fm’ in Stylophora pistillata, Seriotopora hystrix and Acropora formosa (Jones, 2005), the loss of symbiotic algae in Montipora digitata and S hystrix (Jones, 2004), and the detachment of ... tracks, and paths It is also used to control weeds in crops such as pear and apple trees, forests, ornamental trees and shrubs, pineapples, sugar cane, cotton, alfalfa and wheat Furthermore, diuron...

Ngày tải lên: 22/06/2014, 03:20

... Technical report, HDRA, Ryton Organic Gardens, Coventry, CV8 3LG, UK URL: http://www.gardenorganic.org.uk/organicweeds 114 20 Herbicides – Environmental Impact Studies and Management Approaches ... type and variety selection, seed quality and treatment, seeding rate and stand density, seeding date, fertilizer rate and placement, and pest and disease control influenced crop competitiveness and ... HERB, and webHADSS: Decision aids for field crops, Weed Technology 17: 412–420 Benzing, A (2001) Agricultura Orgánica: Fundamentos para la Región Andina [Organic agriculture: principles for the Andean...

Ngày tải lên: 22/06/2014, 03:20

... molecular or biochemical markers and studying nucleic acid or isozyme sequences Isozyme markers have been used * Corresponding Author 224 Herbicides – Environmental Impact Studies and Management Approaches ... needed Studies on the biology, ecology and dynamics of Commelina diffusa and strategies for their management in banana fields are therefore justified as they will provide valuable information for ... relationships, point of origin, and gene flow Studies on genetic diversity both at population and species levels are important for weed management, and represent a source of information about genetic...

Ngày tải lên: 22/06/2014, 03:20

... polymorphism for the loci of the soluble and mitochondrial malate dehydrogenase, as well as for the Acp-1 locus, was not reported here Hence the proportion of polymorphism for C bonariensis and ... be possible to adopt similar strategies and processes for their control In subsequent studies carried out in our laboratory, the polymorphism for the - and esterases loci of E heterophylla plants ... countries worldwide (Heap, 2010; Alcorta et al., 2011) In Brazil, the first sites of resistance 238 Herbicides – Environmental Impact Studies and Management Approaches were reported in Rio Grande Sul...

Ngày tải lên: 22/06/2014, 03:20

... response to external force fields and physiochemical modifications by proteins Advancements and modifications are being made to incorporate more features into these tools, such as fluorescence resonance ... immobilizing multiple DNA molecules in a single run However, more research is needed to attain more reproducible and controllable stretching by this method and to more fully understand the underlying ... Steven Chu [21] visualized single tethered DNA molecules stretched in a uniform flow to investigate viscous forces and hydrodynamic interactions between the DNA molecule and the flowing fluid By measuring...

Ngày tải lên: 22/06/2014, 18:20

... results and have great value for understanding the SC component function Chapter is a conclusion for all my studies 32 CHAPTER 2: Methods and materials 2.1 Single molecule manipulation Single- molecule ... to exert forces and torques on individual molecules or groups of molecules via a pair of magnets It can be used to study mechanical properties of biological macromolecules like DNA and proteins ... experiments can manipulate individual molecules, and characterize single molecule behavior In a typical experiment, the biomolecule is attached to a micromanipulator that works like the spring of a dynamometer;...

Ngày tải lên: 09/09/2015, 11:25

... httEx1-25Q-EGFP (25Q EGFP vector), httEx1-72Q-EGFP (72Q EGFP vector) or httEx1-103Q-EGFP (103Q EGFP vector) Cotransfections were performed using the vectors encoding Hsp70 ⁄ Hdj-1 or Hsp27 Forty-eight hours ... washed with NaCl ⁄ Pi, and incubated with 2¢,7¢-dichlorofluorescein (DCFH-DA), and fluorescence was monitored by FACS cytometry as described in Experimental procedures The fluorescence index was determined ... httEx1-72Q-EGFP or httEx1-103Q-EGFP vectors in combination with vectors encoding for either Hsp70 ⁄ Hdj-1 or Hsp27 Forty-eight hours after transfection, confocal analysis was performed to analyze...

Ngày tải lên: 19/02/2014, 06:20

... and C Sinquin for the extraction and the preparation of the fucoidan fraction and for their experimental advices We are grateful to Professor G J Arlaud for his generous gift of purified C1q and ... CCD camera (C4880 Hamamatsu, and Ixon-Andor) was mounted on the microscope For fluorescence observations, a mercury lamp was used in combination with a filters set for fluorescein (Leica I3) The images ... in chlorobenzene (13% w/w) is put down onto a clean glass cover-slide and spread with a spin coater of in-house design, at 2500 r.p.m for Surfaces are then baked at 165 °C for 40 and stored at...

Ngày tải lên: 16/03/2014, 23:20

... it is important to identify new targets and, at the same time, to develop new approaches for the design of anti-HIV therapy A promising approach is the use of peptides for inhibition or activation ... by the authors Such materials are peer-reviewed and may be re-organized for online delivery, but are not copy-edited or typeset Technical support issues arising from supporting information (other ... are colored red on chain A and in orange on chain B [70] IN and DNA coloring is the same as in the basic tetramer (A) above peptides, LEDGF 401413 is the only one that bound two IN-derived peptides...

Ngày tải lên: 22/03/2014, 16:21

... they play a key role in cell adhesion and motility, formation and stability of proteins structures and receptor– ligand interactions.[3] To further explore the role of solvent hydrogen bonding ... of b-strands A’ and G for unfolding the I27 protein in D2O The pulling coordinate for the separating b-strands is defined as the distance between the first amino acid of strand A’ (Y9) and the ... trajectories of individual I278 proteins in H2O and D2O The force-ramp protocol linearly decreases the force applied to a protein with time and allows for the observation of the full force–length...

Ngày tải lên: 22/03/2014, 18:20

... wave (singlesided total internal reflection fluorescence, TIRF) Prior attempts to marry optical trapping with single- molecule fluorescence failed to achieve spatially coincident fluorescence and ... trapping and singlemolecule fluorescence supply information beyond what can be learned with either technique alone, permitting structure and mechanics to be probed within a single macromolecule ... enzymes Moreover, simultaneous optical trapping and single- molecule fluorescence measurements supply information about the sequence of molecular events Relative timing information can be used, for...

Ngày tải lên: 06/08/2014, 18:20

... repeats and 3, and repeats and (Figure 6) These loops also present potential binding surfaces for regulatory proteins Most of the AtPuf proteins that were expressed in epidermal cells as fluorescent ... Protean II, BioRad) at 100 V for 45 minutes at 4°C Gels were dried, exposed to a storage phosphor screen for 612 hours, and the screens scanned using a phosphorimager (Molecular Imager FX, BioRad) Densitometry ... Authors’ contributions DGM and PPCT designed the experiments PPCT, IHB-N and MWCT conducted the modeling analysis PPCT, DMS and MWCT performed the bioinformatics analysis PPCT, DGM and ALA performed...

Ngày tải lên: 12/08/2014, 03:21

... the lentiviral vector surface Having the targeting molecule more efficiently incorporated onto the vector surface could enhance the binding of the vector to the cognate receptor on the target cell ... Incorporation of both FM and antibody onto the vector surface (A) 293T (top) and 293T/CD20 (bottom) cells were incubated with either FUGW/SC2H7-A2/SGN (left) or FUGW/VSVG (right) at 4°C for hour, ... FMs and screening for a FM with higher pH fusion activity Another approach to optimize this two -molecule targeting strategy is to engineer the targeting antibody to be more efficiently incorporated...

Ngày tải lên: 12/08/2014, 04:21

... as Punias and Hooke Conclusions Single- molecule force spectroscopy is a promising method for measuring the unfolding forces of single molecules and cells SMFS can analyze membrane proteins in ... before the main peak there is a helix unfolding gradually step-by-step, and then a helix unfolds in an all -or- none manner [14,15] For example, helices F and G neighbor helices A and B and the former ... transmembrane proteins Bioinformatics (Oxford, England) 2010 Bosshart PD, Casagrande F, Frederix PLTM, Ratera M, Bippes CA, Mueller DJ, Palacin M, Engel A, Fotiadis D: High-throughput single- molecule force...

Ngày tải lên: 12/08/2014, 17:20