peo modified poly l amino acid micelles for drug delivery

... gastrointestinal tract infections; localized delivery of cytotoxic and anti-angiogenic drugs for solid tumors in novel biodegradable polymeric nanoparticles; intracellular delivery systems for drugs and ... vasculature combined with drug delivery Other approaches using polymeric micelles have focused on targeting the acidic extracellular pH of the tumor microenvironment.37 Polymeric micelles have also ... Lipid-core micelles for targeted drug delivery, Curr Drug Deliv., 2(4), 319–327, 2005 33 Zeng, F., Liu, J., and Allen, C., Synthesis and characterization of biodegradable poly( ethylene glycol)block -poly( 5-benzyloxy-trimethylene...

Ngày tải lên: 04/06/2014, 14:30

... in chloroplasts J Plant Physiol 163, 333–347 Soll J & Schleiff E (2004) Protein import into chloroplasts Nat Rev Mol Cell Biol 5, 198–208 Qbadou S, Becker T, Mirus O, Tews I, Soll J & Schleiff ... reaction using radiolabelled precursor proteins depleted of ATP by gel-filtration as well as chloroplasts stored in the dark to deplete intraorganellar ATP (Fig 2A) Subsequently, import reactions ... chloroplasts corresponding to 15 lg chlorophyll Import reaction was performed for 12 at 25 °C for pTic22 and pMGD1 and for the pSSU control After import, chloroplasts were subjected to thermolysin...

Ngày tải lên: 23/03/2014, 07:20

... cells in vivo In each panel, the left column is a falsecolour image of the GFP channel, the middle column is chlorophyll autofluorescence and the right column is the GFP and chlorophyll channels ... 2004 As well as the role of the transit peptide itself, several other processes may play a role in the specificity of targeting to organelles, including interactions with organellar surface lipids, ... the passenger GFP to plastids in either leaf cells (Figs and 5A) or nonphotosynthetic cells (Fig 4) Similarly, this caused no alteration in the dual-targeting to chloroplasts and mitochondria...

Ngày tải lên: 23/03/2014, 12:20

... of cell surface cholesterol in living cells with EGFP-D4 (A) MOLT-4 cells were incubated with EGFP-D4 for at room temperature Labeled cells were observed under a fluorescent microscope (B) MOLT-4 ... visualize cell surface cholesterol in both living and fixed cells in only one step Therefore staining with EGFP-D4 has the advantage that the distribution and movement of cholesterol in living cells ... with antiwhole h-toxin antibody Preparation of lipid vesicles Phospholipids [dioleoylphosphatidylcholine (DOPC) or brominated distearoylphosphatidylcholine (Br-DSPC)] alone or : (mol/mol) mixtures...

Ngày tải lên: 23/03/2014, 21:20

... (Uppsala, Sweden) The human acute lymphoblastic leukemia CEM cell line and human colon adenocarcinoma LS 174T cell line are available from the American Type Culture Collection (Rockville, MD) ... procedures for producing highly specific monoclonal antibodies (MAbs) are well established In principle it should be possible to deliver liposomes to any cell type as long as the cells are accessible ... antiviral protein molecules covalently linked to each monoclonal antibody molecule Leuk Lymphoma 18, 93–102 17 Beintema, J J., Schuller, C., Irie, M., and Carsana, A (1988) Molecular evolution...

Ngày tải lên: 10/04/2014, 22:17

... accumulation of conjugates in the cells Effectively, as for OLF–Cur loaded macrophages, as a result of cell activation, the cells had more irregular nuclear shape, more voluminous cytoplasm with ... core–bilayer shell nanoparticle: a novel vehicle for entrapment of poorly water-soluble drugs, Polymer 50 (2009) 3508–3515 T Rheinlander, R Kotitz, W Weitschies, W Semmler, Different methods for ... resist well to opsonizations and therefore are cleared slowly, our choice was based on well known hydrophilic chitosan (CS) and oleic acid (OL), rationalizing on the fact that CS is an excellent...

Ngày tải lên: 02/07/2014, 14:14

... “gold standard.” The most rigorous method is double blind and placebo controlled, but single-blind (ie, the patient) and open-label challenges can be performed Doubleblind placebo-controlled ... cow’s milk allergy with intestinal symptoms J Allergy Clin Immunol 1996;98:781–9 23 Van Sickle GJ, Powell GK, McDonald PJ, Goldblum RM Milk- and soy protein-induced enterocolitis: evidence for lymphocyte ... of food allergy Curr Allergy Asthma Rep 2005;5:86–90 28 Brown K Eosinophilic enteritis In: Altschuler S, Liacouras C, editors Clinical pediatric gastroenterology Philadelphia: Churchill Livingstone;...

Ngày tải lên: 08/08/2014, 21:20

... cell lines by increasing apoptotic cell death Materials and methods Cell lines Human colorectal cancer cell lines (SW480, HT-29, LoVo and Colo320) and one human intestinal epithelial cell line ... cells (5.0×103/well) were seeded into five 96-well culture plates with each plate having all three kinds of cells (6-parallel wells/group) On each day, 200 L MTT (5 mg/mL) was added to each well, ... expression levels of Bcl-xL mRNA and protein were obviously higher in human CRC cell lines than in human intestinal epithelial cell line (Figure.1A and B) Among CRC cell lines, the expression levels...

Ngày tải lên: 09/08/2014, 02:21

... afterwards, rats were killed and popliteal lymph nodes (PLN), inguinal lymph nodes (ILN), spleen, and peripheral blood were isolated Single-cell suspensions were prepared by mechanically forcing the organs ... control liposomes or anti-CD134 liposomes Rats were killed 30 later, and popliteal lymph nodes (PLN), inguinal lymph nodes, and spleens were isolated (a) Cells were stained for CD4 and Tcell antigen ... anti-mouse immunoglobulin to label the cellbound mAbs or liposomes Finally, cell-associated fluorescence was measured For analysis of the interaction of CD4+ T cells and liposomes by confocal microscopy,...

Ngày tải lên: 09/08/2014, 06:22

... investigated magnetically retainable drug delivery systems, an approach as yet clinically unexploited despite the intense need for the development of novel i-a delivery modalities Thus, our aim ... fixed with 4% formaldehyde in PBS and used for histological analysis All experimental procedures on animals reported in this paper were performed in compliance with Swiss federal law on the protection ... the variability of the animal model response and the rather small number of animals per group could partially explain this trend This observation questions the reliability of the conclusions drawn...

Ngày tải lên: 09/08/2014, 14:20

... NP40, 250 mmol /L NaCl, mmol /L EDTA, mmol /L EGTA, mmol /L DTT, 0.5 mmol /L phenylmethylsulfonyl fluoride, 20 mmol /L b-glycerophosphate, mmol /L sodium vanadate, and μg/mL leupeptin] Cell extracts were ... USA) Cell culture Two cervical cancer cell lines HeLa and Siha, an ovarian cancer cell line SKOV3, and a non-small cell lung cancer cell line A549 were from American Type Culture Collection (ATCC, ... intracellular ROS accumulation in Siha cells, A549 cells, and SKOV3 cells Siha cells, A549 cells, and SKOV3 cells were treated with saikosaponin-a (10 μM) or saikosaponin-d (2 μM) for 30 respectively...

Ngày tải lên: 10/08/2014, 10:20

... multiple obstacles to their social tenure People with addictions are welcomed into an entry-level role providing life saving healthcare They have access to a range of "low threshold" vocational ... Additional material Additional file Open letter written to the Office of National Drug Control Policy Letter to Office of National Drug Control Policy from Ranking Member Henry A Waxman on behalf of ... meant to enforce a kind of personal responsibility for people with addictions This would not be unlike making an alcoholic bring a wine bottle back before they could purchase another bottle of wine...

Ngày tải lên: 11/08/2014, 18:21

... before Lane = RNA from LLC transduced with AAVNk4 particles, lane = RNA from untransduced LLC cells, lane = H2O template control (b) AAV transduction of LLC tumours In vivo luminescence in LLC ... with pSelectBlasti-2BhIL32b, lane = pSelectBlasti2BhIL32b DNA, lane = RNA from untransfected LLC cells, lane = H2O template control (b) Transfection of LLC tumours in vivo In vivo luciferase activity ... Macroscopic LLC metastatic lung nodules Nodules appear as dark red spots on freshly excised lung, or light yellow colour on lungs fixed in Bouin's solution O/N Cross sections show the morphological appearance...

Ngày tải lên: 14/08/2014, 19:22

... stimulates glycolytic lactate generation (2) mGluR activation also leads to glycolysis and lactate production, and neuronal activity drives astrocyte glycogenolysis (3) and eventual lactate formation ... (activity) by supplying neurons with substrates for oxidative phosphorylation (lactate, glutamine (Gln)) and glutamate (Glu) replenishment (glutamine), and by signaling changes in local blood flow at the ... intracellular lactate for extracellular PGE2 These findings establish lactate as a novel operator of the astrocyte vasomotor switch and are consistent with the ability of lactate to augment neuronal-activity-induced...

Ngày tải lên: 14/08/2014, 21:20

... (Stoelting & Hillor 2006) Generally, for regulation of hypnosis, inhalational drug isoflurane and intravenous drug propofol are most commonly used Schwilden et al (1987) and Schwilden & Schuttler ... strategies applied for regulation of several variables by infusing various drugs in clinical anesthesia will be discussed in detail in chapter Most of the closed-loop systems are still under development ... proportional-integral-derivative (PID) controllers as well as model based controllers However, there is a need for comprehensive evaluation of closed-loop systems to establish their safety, reliability...

Ngày tải lên: 14/09/2015, 08:25

... GFP-expressing cells almost exclusively localized in the inner limiting membrane (ILM) and ganglion cell layer (GCL) In order to successfully identify the phenotype of the GFP+ cells, we performed immunohistochemical ... Immortalization of bovine umbilical vein endothelial cells: a model for the study of vascular endothelium Eur J Cell Biol 2002, 81(1):1-8 Strahl BD, Allis CD: The language of covalent histone modifications ... injected with BacFLT-GFP, (n = 10 animals) Abbreviations: RPE, retinal pigment epithelium GCL, ganglion cell layer; INL, inner nuclear layer; and ONL, outer nuclear layer; ILM inner limiting membrane...

Ngày tải lên: 18/06/2014, 18:20

... GFP-expressing cells almost exclusively localized in the inner limiting membrane (ILM) and ganglion cell layer (GCL) In order to successfully identify the phenotype of the GFP+ cells, we performed immunohistochemical ... Immortalization of bovine umbilical vein endothelial cells: a model for the study of vascular endothelium Eur J Cell Biol 2002, 81(1):1-8 Strahl BD, Allis CD: The language of covalent histone modifications ... injected with BacFLT-GFP, (n = 10 animals) Abbreviations: RPE, retinal pigment epithelium GCL, ganglion cell layer; INL, inner nuclear layer; and ONL, outer nuclear layer; ILM inner limiting membrane...

Ngày tải lên: 20/06/2014, 01:20

... custom 12-plex human cytokine FLEX® kit (Upstate, Millipore, Billerica, MA, USA) that included beads specific for TNFα, IL-1α, IL-1β, IL-6, IL12p40, IL-12p70, IL-15, granulocyte- macrophage colony-stimulating ... analysis [19] Non-normalized datasets were used for all analyses in the article Although this approach limits the ability to detect differences in the low signal intensity range, the rationale for ... VLNRLKVGLQV [Swiss-Prot:P10809] Osteoglycin(176–195) NQLLKLPVLPPKLTLFNAKY [Swiss-Prot:P20774] Serine protease 11(433–452) VIISINGQSVVSANDVSDVI [Swiss-Prot:Q92743] Biglycan(247–266) EDLLRYSKLYRLGLGHNQIR...

Ngày tải lên: 09/08/2014, 14:21

... from Life Technologies All other chemicals were analytical-grade products of POCh-Gliwice (Gliwice, Poland) All measurements were performed in buers prepared in water puried with the Millipore ... polarizers were also used All measurements were performed at 20 0.2 C Before measurements, all samples were ltered through a microporous lter (0.45 lm; Millipore) to remove insoluble impurities ... Shimadzu LC-9A pump equipped with FCV-9AL low-pressure proportioning valve, (b) a Knauer A0263 manual injector equipped with a 100-lL loop, (c) a Supelcosil LC-318 HPLC (5 lm) cartridge column (250...

Ngày tải lên: 20/02/2014, 23:20