pde4b 4d and pde7a aon against cigarette smoke induced lung inflammation in mice and comparison with roflumilast
Báo cáo y học: " Expression of transforming growth factor- in chronic idiopathic cough" pdf
Ngày tải lên :
12/08/2014, 14:20
... observed also in bronchoalveolar lavage fluid [25] and increased neutrophils in induced sputum [26], with increased concentration of histamine, PGD2 and PGE2, together with TNF and IL-8 in induced ... Sections were incubated with the avidin/biotinylated peroxidase complex for 30 min, followed by chromogenic substrate diaminobenzidine for min, and then counterstained in haematoxylin and mounted ... additional investigations including bronchoscopy and intensive therapeutic trials for asthma, gastro-esophageal reflux and postnasal drip/rhinosinusitis, and were labelled as 'idiopathic' Only ex-smokers...
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Báo cáo y học: " Smoking status and anti-inflammatory macrophages in bronchoalveolar lavage and induced sputum in COPD" doc
Ngày tải lên :
12/08/2014, 13:22
... ex-smoking and never smoking COPD patients with GOLD stage II-III [36] We did not find a difference in BAL IL-6 and IL-8 and sputum IL-6 between current smokers and ex-smokers with COPD, in line with ... higher in BAL from ex-smokers than in current smokers with COPD In addition, the percentage of anti-inflammatory macrophages was higher in BAL than in induced sputum, indicating a predominance ... recovery in smokers was higher compared to ex-smokers (45 (16)% and 35 (19)%, p = 0.039 respectively) Smoking status and CD163+ macrophages in BAL and induced sputum DTT used to liquefy the induced...
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Báo cáo y học: "Alterations of alveolar type II cells and intraalveolar surfactant after bronchoalveolar lavage and perfluorocarbon ventilation. An electron microscopical and stereological study in the rat lung" pdf
Ngày tải lên :
12/08/2014, 15:20
... (Fig 1) and an increase in PaCO2 despite of an increase in PIP and subsequently higher tidal volumes (Tab 1) A significant improvement in oxygenation was found within minutes after starting partial ... phosphatidylcholine in lung lavage material did not change after one hour of liquid ventilation Using labeled choline, Steinhorn and colleagues showed a higher content of labeling in the lung and BAL ... Healthy in the volume of lamellar bodies per cell with a subsequent decrease in the intracellular surfactant content per lung (Tab 3) Taking the qualitative findings and the stereological data on intraalveolar...
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báo cáo hóa học: " Comparative analysis of induced sputum and bronchoalveolar lavage fluid (BALF) profile in asbestos exposed workers" potx
Ngày tải lên :
20/06/2014, 00:20
... SI, Cantin A, Crystal RG: Characterization of the lower respiratory tract inflammation of nonsmoking individuals with interstitial lung disease associated with chronic inhalation of inorganic ... presented; *ABs not found in any reported PPE use useful in providing an insight both for inhalation of dusts and inflammatory processes in the lung However its usefulness for screening of workers should ... smoking, and respiratory functional findings in the study population are shown in Table The data are presented by smoking status due to its significance in the current study Smokers had smoked...
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Báo cáo y học: "Analysis of bronchoalveolar lavage fluid proteome from systemic sclerosis patients with or without functional, clinical and radiological signs of lung fibrosis" pot
Ngày tải lên :
09/08/2014, 08:22
... pathways, including amplifying the inflammatory process by inducing the extravasion of neutrophils and the production of IL-8, inducing an inflammatory reaction in the endothelia, and/ or stimulating lung ... retinol-binding protein GM 20 Transthyretin GM, Ab 21 Immunoglobulin-binding factor GM 22 Calgranulin A GM 23 Hemoglobin β chain GM 24 ERP60 (protein disulfide isomerase) GM, Ab 25 α1-antitrypsin ... glycoprotein, calumenin, cytohesin-2, Cal B, and mtDNA TOP1 Haptoglobin and α1-acid glycoprotein are positive acutephase proteins, whose plasma concentrations increase during inflammation [36] The increased...
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Báo cáo y học: " Assessment of pulmonary antibodies with induced sputum and bronchoalveolar lavage induced by nasal vaccination against Pseudomonas aeruginosa: a clinical phase I/II study" pot
Ngày tải lên :
12/08/2014, 15:21
... (BALF) and serum (upper panel), between induced sputum and serum (middle panel) and between induced sputum and BALF (lower panel) obtained weeks after completion of the vaccination schedule in n ... bronchoalveFigure sputum and BALF OprF-OprI-specific IgG (left colms) and induced of the and serum (middle in n = 12 between induced Individual levels of OprF-OprI-specific IgG (left colms) and IgA values ... fluid (BALF, A and C) and induced sputum (IS, B and D) of n = 12 healthy volunteers vaccinated with nasal primary and a nasal or systemic booster vaccination Specimen were obtained weeks after...
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Báo cáo y học: " Functional and phenotypical comparison of myofibroblasts derived from biopsies and bronchoalveolar lavage in mild asthma and scleroderma" ppsx
Ngày tải lên :
12/08/2014, 16:20
... minutes After permeabilization in 0.5% Triton X-100 in PBS for minutes, and blocking with 1% BSA in PBS for 30 minutes, the cells were incubated for 30 minutes with Alexa Fluor™ 488 phalloidin ... facilitated by proteins associated with the actin cytoskeleton and intracellular signaling pathways involving small GTPases such as RhoA and Rac1, which induce formation of stress fibers and focal adhesions ... cultured for hours within a cloning cylinder The cylinder was removed and the fibroblasts were allowed to migrate for 48 h The cells were fixed in 1% glutaraldehyde and stained for hours in 0.5% crystal...
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Báo cáo y học: " The predictive role of serum and bronchoalveolar lavage cytokines and adhesion molecules for acute respiratory distress syndrome development and outcome" pptx
Ngày tải lên :
12/08/2014, 18:20
... Thr389 in p70 S6 kinase was increased in the presence of insulin after 15 of incubation and remained increased after 16 hours Rapamycin abolished the phosphorylation of Thr389 in both control and insulin-treated ... Agati JM, Yeagley D, Quinn PG: Assessment of the roles of mitogen-activated protein kinase, phosphatidylinositol 3-kinase, protein kinase B, and protein kinase C in insulin inhibition of Page of ... p44/42 mitogen-activated protein kinase (MAPK) pathway and the phosphatidylinositol 3-kinase (PI 3-kinase) pathway [8] Insulin initially binds to the insulin receptor causing autophosphorylation of...
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Báo cáo y học: " The predictive role of serum and bronchoalveolar lavage cytokines and adhesion molecules for acute respiratory distress syndrome development and outcome" doc
Ngày tải lên :
12/08/2014, 18:20
... have been found to be increased in BALF in the early phase of ARDS, including endotoxin-binding proteins, tumour necrosis factor alpha (TNF-α), interleukin (IL)-1, IL-6, chemokines, adhesive molecules ... metalloproteinases and their inhibitors [2–4] Leukocyte activation and the free radical release, proteases, arachidonic acid metabolites, inflammatory and anti-inflammatory cytokines and TNF-α result in ... important role in cell interactions during inflammatory responses ICAM-1 is induced by lipopolysaccharide (LPS) and cytokines such as IL-1, TNFα [14–16], and subsequent interactions with lymphocyte...
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Báo cáo y học: "Evaluation of Fractional Analysis of Bronchoalveolar Lavage Combined with Cellular Morphological Features"
Ngày tải lên :
03/11/2012, 11:48
... in 96 patients with a variety of interstitial lung diseases, including 37 patients with IIP, 10 sarcoidosis, eosinophilic pneumonia, and others Distribution of mast cells in HP patients examined ... of which are shared with eosinophils and account for the strong, temporal association of their influx into tissues 11 An intriguing point in our study was the cytological finding of Masson bodies ... organ involvements of sarcoidosis including skin, eyes and/ or heart As to smoking habits, 31 (40.8%) were current smokers, 37 patients (48.7%) had never smoked or were past smokers Table Clinical...
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Báo cáo hóa học: " Respiratory function and bronchial responsiveness among industrial workers exposed to different classes of occupational agents: a study from Algeria" potx
Ngày tải lên :
20/06/2014, 00:20
... deterioration of lung function with advancing age in welders compared with controls In a longitudinal study of welders and caulker-burners with follow-up of retired workers, Chinn and colleagues ... impairment (FVC and FEV1 < 80% predicted and FEV1/FVC > 0.70) was present in 11 subjects (2.0%, all smokers), with subject in the control group, subjects in the mineral dust group and in each of the ... paid better and this can be expected to lead to better nutrition and lifestyle [34] In conclusion, baseline FEV1 was lower in smokers and, independently of smoking status, lower in workers exposed...
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Báo cáo y học: "Proteome analysis of bronchoalveolar lavage in Pulmonary Langerhans cell histiocytosis" pptx
Ngày tải lên :
10/08/2014, 09:22
... protective role against cigarette smoke- induced lung inflammation (41) It protects cells and enzymes against oxidative damage by catalyzing the reduction of hydrogen peroxide, lipid peroxides and organic ... antioxidant protein down-regulated in BAL of PLCH patients with respect to smoker controls It plays a protective role against cigarette smoke- induced lung oxidative damage (42, 43) and reacts against reactive ... protects against cigarette smoke- induced lung inflammation in mice Am J Physiol Lung Cell Mol Physiol 2010;299(3):L425-33 Morrison D, Rahman I, Lannan S, MacNee W Epithelial permeability, inflammation, ...
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Báo cáo y học: "CD8+ T lymphocytes in bronchoalveolar lavage in idiopathic pulmonary fibrosis" ppt
Ngày tải lên :
11/08/2014, 08:21
... the inflammatory cells including neutrophils, macrophages, eosinophils and T lymphocytes (TLs) in the modulation of tissue injury in IPF [5-8] Recently, we shown that in IPF tissue, infiltrating ... paucity of interstitial inflammation, the role of inflammatory response in the modulation of tissue injury and fibrosis still remains under investigation [19,20] In the present study among all inflammatory ... twice with cold phosphate buffered saline (PBS) and resuspended in ml RPMI 1640 medium (Gibco; Grand Island, NY) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (FBS; Gibco; Grand...
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Báo cáo y học: " Open Access No evidence of altered alveolar macrophage polarization, but reduced expression of TLR2, in bronchoalveolar lavage cells in sarcoidosis" doc
Ngày tải lên :
12/08/2014, 11:22
... possibly indicating that these patients respond to a particular ligand that specifically binds to TLR2 The finding of a tendency to a smaller LPS -induced increase in IL-23 expression in sarcoidosis ... (Sigma-Aldrich, Irvine, U.K.), supplemented with 1% penicillin streptomycin (Invitrogen Corporation, Paisley, Scotland), 1% L-glutamine (Sigma-Aldrich, Irvine, U.K.) and 2% heat-inactivated human ... epidemiological studies and findings of DNA from mycobacteria [12] and propionibacteria [13] and mycobacterial antigens [14], in sarcoidosis tissue and lymph nodes indicate an infectious cause This...
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Báo cáo y học: "Altered expression of T cell Immunoglobulin-Mucin (TIM) molecules in bronchoalveolar lavage CD4+ T cells in sarcoidosis" docx
Ngày tải lên :
12/08/2014, 14:20
... immunoglobulin and mucin domains and intracellular domains of different lengths TIMs are differentially expressed on Th1 and Th2 cells [9,10] The TIM gene family includes eight genes in mice and three ... mRNA expression and IFN-γ mRNA levels in Löfgren's patients and controls, but not in non-Löfgren's patients This is in line with an imbalanced pulmonary inflammation especially in non-Löfgren's ... syndrome and those without Hypothetical model of inflammatory reactions in sarcoidosis, comparing the immune response in the lungs of patients with Hypothetical model of inflammatory reactions in sarcoidosis,...
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Báo cáo y học: " Bronchoalveolar lavage fluid from preterm infants with chorioamnionitis inhibits alveolar epithelial repair" ppsx
Ngày tải lên :
12/08/2014, 14:20
... entities induce different responses affecting subsequent lung development The notion that antenatal steroid administration further reduces RDS incidence in preterm infants exposed to intrauterine inflammation, ... between infants with and without chorioamnionitis and infants that had and had not received antenatal steroids Chorioamnionitis decreases wound healing capacity of BALF BALF obtained from infants with ... alveolar epithelial injury in vitro, we focused on BALF -induced apoptosis and possible roles for KGF and VEGF, known modulators of lung development and lung injury repair KGF and VEGF were quanti-...
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Báo cáo y học: " The role of γδ T cells in airway epithelial injury and bronchial responsiveness after chlorine gas exposure in mice" pps
Ngày tải lên :
12/08/2014, 15:20
... implicated in irritant induced asthma Our findings indicate that the response to airway injury with chlorine differs between wild type and γδ T cell deficient mice Wild type mice have more inflammation ... macrophages [8] Porcine and rabbit models of Cl2 injury have demonstrated similar histological and lung function findings, where increases in pulmonary resistance and elastance, edema, sloughing of bronchial ... counterstained with methyl green Mouse intestinal tissue was used as a positive control Adjacent tissue sections were stained with hematoxylin and eosin for routine histological examination Morphometry...
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Báo cáo y học: " Proinflammatory cytokine responses induced by influenza A (H5N1) viruses in primary human alveolar and bronchial epithelial cells" ppt
Ngày tải lên :
12/08/2014, 18:20
... at inducing proinflammatory cytokines and chemokines, including IP-10, interferon beta, IL-6 and RANTES, from human primary alveolar and bronchial epithelial cells infected in vitro Previous findings ... * indicates p < 0.01 compared with mock and ** indicates p < 0.05 compared with H5N1/97 and H1N1 infected cells using the Bonferroni multiple comparison test Cytokine and chemokine responses in ... cytokine responses induced by infection of epithelial cells with influenza viruses including H5N1 viruses Human influenza A viruses have been previously reported to induce interleukin (IL-6), interleukin...
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Báo cáo khoa học: "Bronchoalveolar lavage cytological alveolar damage in patients with severe pneumonia" pps
Ngày tải lên :
12/08/2014, 23:20
... the initial manuscript and approved the final version 16 17 18 Acknowledgements We thank Dominique Prat, Severine Girault, Catherine Remy and Sophie Ducuing for their help in retrieving data and ... FGB and OA initiated the project; BG, FJ, OA, FB and REK retrieved the data and participated in patient management; and BG, FJ, FGB and FC analyzed the data All authors were involved in drafting ... organisms from BAL and PTC show an usual profile for such infections, with a predominance of Streptococcus pneumoniae and Haemophilus influenzae in patients with CAP and Pseudomonas aeruginosa, Staphylococcus...
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Báo cáo khoa học: " Is bronchoalveolar lavage with quantitative cultures a useful tool for diagnosing ventilator-associated pneumonia" doc
Ngày tải lên :
13/08/2014, 03:20
... pneumonia cannot explain such a disappointing result, when compared with the 0.5% rate of inappropriate initial therapy reported by Fagon and coworkers in the invasive strategy group [8] The most ... antimicrobial therapy in such a setting can only be obtained when decisions regarding antibiotics are closely linked to bacteriological – both direct examination and cultures – results [12] In the current ... colony-forming units/ml Interestingly, the proportion of ‘confirmed pneumonia’ was 86% in the BAL group and 83% in the endotracheal aspirate group In contrast to previous recommendations concerning...
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