pcr pulsed field gel electrophoresis pfge and box pcr for molecular analysis of enterococci
Báo cáo khoa học: "The use of caspase inhibitors in pulsed-field gel electrophoresis may improve the estimation of radiation-induced DNA repair and apoptosis" pdf
Ngày tải lên :
09/08/2014, 09:20
... the study and drafted the manuscript GP, LL and MB participated in PFGE and in immunofluorescence studies XS provided informatics and support with statistics for data analysis SM, OC, RM and GC ... washed and transilluminated at 302 nm Fluorescence intensity of the DNA was acquired, and transformed to arbitrary units of optical density using a digital imaging analysis system (GelDoc 2000 and ... The GelDoc settings for fluorescence acquisition were adjusted (exposure time and iris aperture) in each gel to obtain smears below saturation levels but Page of Figure PFGE pattern (A) PFGE for...
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GEL ELECTROPHORESIS – PRINCIPLES AND BASICS pdf
Ngày tải lên :
28/06/2014, 15:20
... Lara-Astiaso, Ricardo Amils and Angeles Aguilera Chapter 16 Application of Multiplex PCR, Pulsed- Field Gel Electrophoresis (PFGE) , and BOX -PCR for Molecular Analysis of Enterococci Charlene R Jackson, ... melting of the gel, DNA bands smiling, decrease of DNA bands resolution and fuse blowout Therefore, it is highly recommended not exceed 5-8 V/cm and 75 mA for standard size gels or 100 mA for minigels ... “stacking” gel of low percentage of acrylamide and low pH (6.8) and a separating gel with a pH of 8.8 and much smaller pores (higher percentage of acrylamide) The stacking gel prevents any high -molecular- weight...
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Báo cáo khoa học: "Genomic variability in Potato virus M and the development of RT-PCR and RFLP procedures for the detection of this virus in seed potatoes" ppt
Ngày tải lên :
12/08/2014, 04:21
... were designed and RT -PCR procedures were developed for the specific detection of PVM in various potato samples and for the confirmation of PCR amplicons The efficacy of RT -PCR for indexing seed ... Figure Detection of PVM RNA in potato samples by RT -PCR and confirmation of PCR products by restriction analysis PCR amplicons (520 bp) were produced in RT -PCR using primers PVM3 and PVM4 from RNA ... distinct groups - group I and II Group I consisted of PVM isolates detected and characterized in Italy, Germany, China, Poland and Russia and group II consisted of Canadian and US isolates Isolates...
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Development of methods to improve sensitivity and portability of capillary electrophoresis for the analysis of stabilizers and drugs 1
Ngày tải lên :
14/09/2015, 10:36
... portable capillary electrophoresis system as in the previous chapter, and potentially can be used for field analysis of chiral compounds In addition, a fast method for the analysis of underivertized ... studies of many groups of compounds The improvement of sensitivity and the miniaturization of CE technique are two major trends of capillary electrophoresis which have gain more and more attention ... before sample injection to reduce the length of the concentrated sample zone and increase the peak height by slowing down the forward movement of the neutral sample associated with β-CD-NH2 and...
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Development of methods to improve sensitivity and portability of capillary electrophoresis for the analysis of stabilizers and drugs 2
Ngày tải lên :
14/09/2015, 10:37
... responsible for the electroosmosis and is designated as the ζ–potential Figure 1.1: Charge distribution at the surface of fused silica and formation of the ζ–potential Figure 1.2: Profile of the ζ–potential ... in a single analysis 12 Figure 1.6 Illustration of double layer and reversal of EOF by addition of a quaternary amine to the buffer 1.3 Different Modes of CE 1.3.1 Capillary Zone Electrophoresis ... self-electrophretic mobilities of neutral analytes and thus the only driving force for neutral analyutes is the EOF, which is equal for all neutral 14 components of a sample On the other hand, in MEKC, the...
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Capillary and microchip electrophoresis for the analysis of small biomolecules
Ngày tải lên :
02/10/2015, 12:56
... CAPILLARY AND MICROCHIP ELECTROPHORESIS FOR THE ANALYSIS OF SMALL BIOMOLECULES ELAINE TAY TENG TENG (B.Sc (Hons), NUS) A THESIS SUBMITTED FOR THE DEGREE OF MASTER OF SCIENCE DEPARTMENT OF CHEMISTRY ... even after it is deformed by the application of external forces Hence, soft lithography technique is commonly utilized for the microfabrication of such microchips35 The design of the fluid circuit ... for CE analysis Thus, it will be 11 ideal for routine analysis, e.g in clinical analysis, where large numbers of samples need to be examined 1.4 Project objectives The quantitative analysis of...
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Hamilton–Jacobi–Bellman equations and dynamic programming for power-optimization of radiative law multistage heat engine system
Ngày tải lên :
05/09/2013, 15:28
... Director of the Department of Nuclear Energy Science and Engineering, the Director of the Department of Power Engineering and the Superintendent of the Postgraduate School Now, he is the Dean of the ... increase of the time β t From Figures and 9, one can see that when T1 f = 500 K and T1 f = 1000 K , the optimal Carnot temperature profiles consist of two segments: the heat engines in the former ... Foundation of P R China (Project No 10905093), the Program for New Century Excellent Talents in University of P R China (Project No NCET-04-1006) and the Foundation for the Author of National...
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Tài liệu Assessing Financing, Education, Management and Policy Context for Strategic Planning of Human Resources for Health pdf
Ngày tải lên :
14/02/2014, 09:20
... resources for health, such an exercise is beyond the scope of this tool For a further analysis of workforce planning methods and approaches related to human resources for health, and for a comprehensive ... sources for the assessment of the performance of the health workforce Table Status of human resources for health (HRH): primary indicators of HRH performance Dimension Indicator Benchmark HRH performance ... other professions for highly skilled and motivated graduates and therefore that the quality of the health workforce will be affected by the results of this competition A student’s choice of professional...
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Tài liệu the supply oF money – banK behaViour and the implications For monetary analysis doc
Ngày tải lên :
16/02/2014, 11:20
... to this type of analysis, albeit in a stylised manner See, for example, Brunner, K and Meltzer, A.h., “Some Further Investigations of Demand and Supply Functions for Money”, Journal of Finance, ... entitled “Multiplier analysis of the effect of monetary policy on money supply”) The supply of money – bank behaviour and the implications for monetary analysis See Brunner, K and Meltzer, A., “Money ... “Money Supply”, in Friedman, B and Hahn, F.H (eds.), Handbook of Monetary Economics, Vol. I, North-Holland, Amsterdam, 1990, p 396 Box Multiplier analysis of the effect of monetary policy on money...
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Tài liệu Closing the gap between research and practice: Foundations for the acquisition of literacy pptx
Ngày tải lên :
17/02/2014, 06:20
... well as the analysis of texts and interactions in order to apply critical theories of social organisation, patterns of dominance and control, and avenues for change motivated by cultural and social ... uniform because the same sources A more detailed analysis of the philosophy and practice of whole language teaching in Australia and New Zealand is provided by Hempenstall (1996), and Tunmer and ... measures of reading real words, both regular and irregular (effect sizes of 55, 46 and 60), and on the measures of reading comprehension and listening comprehension (effect sizes of 69 and 40)...
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Tài liệu Taxes and the Economy: An Economic Analysis of the Top Tax Rates Since 1945 docx
Ngày tải lên :
20/02/2014, 19:20
... Figure Share of Total Income of Top 0.1% and the Top Tax Rates, 1945-2010 13 Figure Share of Total Income of Top 0.01% and the Top Tax Rates, 1945-2010 14 Figure Labor Share of Income and the ... Report R42020, The 2001 and 2003 Bush Tax Cuts and Deficit Reduction, by Thomas L Hungerford for an analysis of the Bush tax cuts Peter Diamond and Emmanuel Saez, “The Case for a Progressive Tax: ... 34 A B Atkinson and Andrew Leigh, “Top Income in New Zealand 1921-2005: Understanding the Effects of Marginal Tax Rates, Migration Threat, and the Macroeconomy,” Review of Income and Wealth, vol...
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PATENTS AND MEDICAL BIOTECHNOLOGY: AN EMPIRICAL ANALYSIS OF ISSUES FACING THE AUSTRALIAN INDUSTRY
Ngày tải lên :
13/03/2014, 21:54
... Methods of treatment of humans and animals Article 27(3)(a) of the TRIPS Agreement expressly allows for the exclusion of diagnostic, therapeutic and surgical methods of treating humans and animals ... Exclusion of plants and animals for the purposes of an innovation patent Section 18 (1A), (3) and (4) of the Patents Act 1990 excludes applications for innovation patents (but not for standard patents) ... sequences and their use in diagnosis and prognosis of breast, ovarian and other cancers, therapies and drugs The patents include claims to a whole range of methods for identifying, screening and detecting...
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Blank values, adsorption, pre concentration, and sample preservation for arsenic speciation of environme
Ngày tải lên :
15/03/2014, 23:22
... was used to fill the bottles of different materials The bottles were shaken in the dark at ± ◦ C and 20±1 ◦ C for 24 h, and ml of the solution was taken for the analysis of the arsenic compounds ... analytical possibility for arsenic compounds at the level of ng As l−1 , and has high recovery and low transformation Addition of EDTA and storage in the dark can reduce the transformation among arsenic ... As l−1 able transformation of the arsenic compounds during the pre-concentration process, transformation of arsenic compounds may occur before analysis due to the contact of the samples with...
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Assessing Financing, Education, Management and Policy Context for Strategic Planning of Human Resources for Health pdf
Ngày tải lên :
17/03/2014, 15:20
... resources for health, such an exercise is beyond the scope of this tool For a further analysis of workforce planning methods and approaches related to human resources for health, and for a comprehensive ... sources for the assessment of the performance of the health workforce Table Status of human resources for health (HRH): primary indicators of HRH performance Dimension Indicator Benchmark HRH performance ... other professions for highly skilled and motivated graduates and therefore that the quality of the health workforce will be affected by the results of this competition A student’s choice of professional...
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Báo cáo "Monte carlo simulation by code of MCNP and experimental check for measuring thickness of materials for the specializing system of MYO-101 " ppt
Ngày tải lên :
22/03/2014, 11:20
... of Science, Mathematics - Physics 26 (2010) 43-49 47 Proccessing of the measured results and drawing of graphs are done by software of Origin The fitted equations and saturation thicknesses of ... having outer diameter of 60 mm, inner diameter of 15 mm, thickness of mm, and aluminum incident window with thickness of 0.3 mm, and a photomultiplier working at high voltage of 1300 V; Am-241 source ... activity of 370 MBq (10 mCi) in a disk shape (having overall diameter of mm, overall thickness of mm, active diameter of 4.2 mm, beryllium window with thickness of mm) is placed in well of the...
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Base Working Paper on Strategy and Action Plan for Ensuring Safety of Milk and Milk Products ppt
Ngày tải lên :
24/03/2014, 04:20
... Centre for Analysis and Learning in Livestock and Food whose mandate is to offer services on analysis of milk products and other food products for various safety and quality parameters, and carrying ... purpose of having, in addition to mandatory PFA food standards, separate BIS and Agmark standards for milk products, enforced by BIS and Directorate of Marketing and Inspection, Department of Agriculture ... Exports and imports of milk and milk products Problems in export and import of milk and milk products Objective 3: Current enforcement procedures and Prevention of Food Adulteration (PFA) Act and...
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Pelvic floor muscle training and adjunctive therapies for the treatment of stress urinary incontinence in women: a systematic review doc
Ngày tải lên :
28/03/2014, 14:20
... Evidence' [31] and from the Centre for Reviews and Dissemination, University of York [21] The modified tool developed for this review provides a maximum quality rating score of 23 for RCTs and a maximum ... combinations of physical therapy Total number of positive and statistically significant measures of incontinence (black) and non-significant measures of incontinence (grey) for different combinations of ... programs and protocols appropriate for different subgroups of women eg women of different ages and with different severity of incontinence Additional File Summary of critical appraisal – Randomised...
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The Rise and Fall of the U.S. Mortgage and Credit Markets: A Comprehensive Analysis of the Meltdown pot
Ngày tải lên :
29/03/2014, 07:20
... The Rise and Fall of the U.S Mortgage and Credit Markets: A Comprehensive Analysis of the Meltdown, published by John Wiley & Sons It offers a brief preview of the extensive data and analysis ... recounting of the mortgage meltdown and the ensuing financial crisis, along with policy recommendations for moving forward The Rise and Fall of the U.S Mortgage and Credit Markets: A Comprehensive Analysis ... before their rates reset Foreclosures rose sharply, especially in Arizona, California, Florida, and Nevada One-third of homes sold between the third quarter of 2007 and the second quarter of...
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Báo cáo khoa học: Molecular analysis of the interaction between cardosin A and phospholipase Da Identification of RGD/KGE sequences as binding motifs for C2 domains pdf
Ngày tải lên :
30/03/2014, 11:20
... terms of charge and is positioned at the tip of a loop and is therefore reminiscent of RGD sequences present in integrin-binding molecules because of its exposed location On the basis of these structural ... by SDS ⁄ PAGE, and amino-acid sequence information was obtained by MS analysis MS analysis For identification of proteins purified by NHFRGDHTK– EAH Sepharose 4B affinity column, bands were excised ... AWGCRTCYTGRAT-3¢ (reverse) for the sequence IQDAYINA, 5¢-GARCCWTGGCAYGAYATYCAYWS-3¢ (forward) for EPWHDIHS and 5¢-ATGATGATYGTKGA YGAYGARTA-3¢ (forward) for the sequence MMIVD DEY Based on the PCR- amplified...
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Taxes and the Economy: An Economic Analysis of the Top Tax Rates Since 1945 (Updated) pptx
Ngày tải lên :
31/03/2014, 05:21
... also by the quantity and quality of the labor force The indicators of the quantity and quality of the labor force include the change in the proportion of the population of the population with ... Tax Rate Share of Income of Top 0.1% Share of Income of Top 0.1% Fitted values Fitted values Source: CRS analysis of Piketty and Saez data Note: The vertical axis is the share of total income ... 47 A B Atkinson and Andrew Leigh, “Top Income in New Zealand 1921-2005: Understanding the Effects of Marginal Tax Rates, Migration Threat, and the Macroeconomy,” Review of Income and Wealth, vol...
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