pcr cloning and sequencing

... both rice and Arabidopsis thaliana, and are distributed non-randomly throughout the genome [30-32] Clustering of R genes is known to facilitate tandem duplication of paralogous sequences and generation ... were subsequently designed for SNP discovery Amplicon cloning and sequencing For specific homologous and heterologous R gene-derived primers, PCR amplicons were generated using template genomic ... including barley, wheat, rye and oat [33,59] Results Strategies for specific R gene isolation Three strategies (empirical approaches based on heterologous PCR and degenerate PCR, and a bioinformatic discovery...

Ngày tải lên: 12/08/2014, 03:20

... Vertical bars indicate the standard deviations for the random selection method The standard deviations for the information and hub selection curves are less than five and are not shown for clarity ... NIGMS grant GM070743-01 and NSF grant CCF-0425926 T.I was supported by a David and Lucille Packard Fellowship award C.Y and T.J were supported in part by NIH grant(s) GM68762 and GM69676 10 11 12 ... combination of previously documented and novel findings First, in agreement with previous literature, we confirm that MSN4 and HAP4 are inducers [27,28] and that SOK2 is a repressor [29] For...

Ngày tải lên: 14/08/2014, 14:21

... evolutionary aspects see Wray et al [10] and for a review from the bioinformatics perspective see Wasserman and Sandelin [11] A motif is a subsequence of DNA of between and 20 base pairs (bp) of fixed ... real and random promoterwise alignments select this threshold We performed simulation studies with random sequence that showed that bit scores >20 bits are extremely rare when aligning randomly ... mammalian motif and a fish ortholog of a motif instance was clearly nonrandom, as judged by a hypergeometric probability of the cooccurrence When we used randomized motif libraries or randomized ortholog...

Ngày tải lên: 14/08/2014, 15:20

... consent and the protocol was approved by the Ethics Committee Baseline and annual X-rays were provided and assessed by a centralised procedure (Pr C Roux, Cochin Hospital, France) The prevalent and ... its first version had 168 questions and the Osteoporosis Functional Disability questionnaire (OFDQ) [4] focused mainly on pain and handicap rather than QoL and was not generated using patient ... (M0) and then every six months using the SF-36 and QUALIOST® The 12 countries of the SOTI trial were Australia, Belgium, Denmark, France, Germany, Greece, Hungary, Italy, Poland, Spain, Switzerland...

Ngày tải lên: 20/06/2014, 15:20

... Neuropathies Katrin Hahn and Ingo Husstedt Chapter Spondylodiscitis and HIV – Diagnosis and Treatment Strategies 121 Jan Siewe, Kourosh Zarghooni and Rolf Sobottke Chapter Acute Abdomen and HIV Infection ... diabetes, and use of lipid lowering and hypertension therapy 20,775 HIV-infected and 215,158 HIV negative members contributed to 90,961 and 1,133,333 persons-years (py) respectively HIV-infected and ... Atazanavir/ritonavir ↑ LDL-c TG and no change HDL-c Darunavir/ritonavir ↑ TC, LDL-c, TG and no change HDL-c Fosamprenavir/ritonavir ↑ TC, LDL-c, TG and no change HDL-c Indinavir ↑ TC, LDL-c, TG and Lopinavir/ritonavir...

Ngày tải lên: 27/06/2014, 18:20

... and will reduce our screening and analysis efforts to the principal components and actors Strategies Suggestions by Firestein and Pisetsky [41] underline the importance of an understandable and ... retroviral transcripts in renal tubules and also in brain and lung tissue Azuma and colleagues used microarrays for the detection of new candidates in salivary gland tissue from the MLR/MpJ-lpr/lpr ... invasion and bone resorption, also demand the identification of targets to directly inhibit destruction and/ or to induce regeneration and repair A deeper knowledge of pathophysiological networks and...

Ngày tải lên: 09/08/2014, 01:23

... (left) and the robustness of predictions in the face of increasing noise levels (right) these unsuccessful candidates early and at low cost Conversely, careful selection and optimization of candidate ... assay design rules and contributed his experience with COBRA, bisulfite SNuPE, bisulfite Pyrosequencing, MSP, MethyLight and MeDIP-qPCR He also provided experimental data and performed extensive ... and candidate biomarkers (Additional data file 1); the XML-based PMML model that MethMarker uses for exporting, importing and storing candidate biomarkers (Additional data file 2) importing and...

Ngày tải lên: 09/08/2014, 20:20

... design and molecular biology, and facilitated the 2GS procedures MB and LC provided bioinformatics support for the 2GS data CH analysed the mapping data CB and JAM provided sequencing and genotyping ... from developing fruit and buds, and Sanger-sequenced several thousand ESTs [13,14] From these libraries, forty-three SSR and sixteen SNP markers have been mapped genetically and, together with AFLPs, ... sufficient coverage to identify over 7,000 SNPs and 3,000 SSRs Below we discuss the attributes of the assembled contigs and singletons and the utility of the SNP and SSR markers to provide an improved...

Ngày tải lên: 11/08/2014, 11:21

... contributions SL and FB planned and supervised the work CC, AM, BM and AH carried out the molecular genetic studies; EP carried out phylogenetic and linkage analyses All authors read and approved ... level in genes of known biochemical functional is useful for candidate Isolation and cloning of a full length HQT cDNA of globe artichoke and cardoon CODEHOP [37] was used to target conserved acyltransferases ... female and male maps (Fig 7a) A further 14 markers were assigned to the female LG5: four microsatellites (CELMS-24, -36, -44 and CMAL-24), three S-SAPs (cyre5 markers) and AFLPs, covering 62.1 cM and...

Ngày tải lên: 12/08/2014, 03:20

... interdisciplinary team Crit Care Med 2006, 34:211-218 Horan TC, Andrus M, Dudeck MA: CDC/NHSN surveillance definition of health care-associated infection and criteria for specific types of infections in the ... Intensive Care Med 2004, 30:996-997 doi:10.1186/cc8189 Cite this article as: Aardema LM, et al.: Value and price of ventilator-associated pneumonia surveillance as a quality indicator Critical Care 2010,...

Ngày tải lên: 13/08/2014, 20:21

... hit ratio, and this is used to rank the confidence levels of data set matches reports Where N and M are numbers of concordant and discordant matches, respectively, and Siq and Sis (Sjq and Sjs ) ... include those reported by Staunton and coworkers [13] (dataset A), Butte and colleagues [12] (dataset B), Ross and coworkers [14] (dataset C), and GDS89 Datasets A and B were generated using Affymetrix ... described in the Materials and methods section (below) Design and validation of EXALT The EXALT system consists of four components (two program pipelines, SigDB, and search engine) and a web interface...

Ngày tải lên: 14/08/2014, 07:22

... STAT4 and STAT1 genes on human chromosome and Danio rerio chromosomes 22 and Synteny between regions containing STAT4 and STAT1 genes on human chromosome and Danio rerio chromosomes 22 and Genes ... al R251.21 CS and ML conducted BLAST searches, made alignments and phylogenetic trees, made the figures and wrote the text MC identified the cytokine and cytokine receptor genes and analyzed their ... expressed in the myeloid and lymphoid lineages of zebrafish Immunogenetics 2006, 58:31-40 Bobe J, Goetz FW: Molecular cloning and expression of a TNF receptor and two TNF ligands in the fish ovary...

Ngày tải lên: 14/08/2014, 08:20

... (Nsp and Sty) from the Broad Institute and Affymetrix, respectively Again, the gold standard is HapMap calls (c) ADPs are plotted for the 32 and 16 high quality replicates hybridized on XBA and ... HapMap and Affymetrix and, thus, these SNPs were corrected by hand For the replicate data, high quality chips (operationally defined as having an SNR greater than 4.5 and 2.36 for the 100K and 500K ... Hubbell and Chuck Sugnet for helpful discussions; and finally, Ben Bolstad, James MacDonald, Seth Falcon, Marvin Newhouse, Robert Gentleman, Jiong Yang, and Fernando Pineda for help with hardware and...

Ngày tải lên: 14/08/2014, 08:21

... recombination and the position of cross-overs Using in silico and in vitro analyses, Sapienza’s group have shown a relationship between increased rates of meiotic recombination and genomic imprinting ... several generations of offspring He and his colleagues postulate that maternal nutrition before and during pregnancy may affect the establishment of CpG methylation and the life-long expression of ... CpG island methylation rate with 90% accuracy, which was later confirmed by in vitro analyses This study revealed that the DNA composition of CpG islands, the sequence, the structure and the...

Ngày tải lên: 14/08/2014, 17:22

... where standard instruments, such as oscilloscopes, signal generators and logic analyzers are used The standard measurement equipment is also referred to as bench equipment, and the validation and ... transmitter power, the timing jitter, and the method of demodulation and decoding In baseband transmission, the data and clock are combined on the transmitter side and transmitted as digital waveforms ... Verma, Bill Burchanowski, and Sandeep Kumar for collaborating on a work that led to co-authoring one paper We also appreciate the technical support and help from the whole PHY and Storage team at Agere/LSI,...

Ngày tải lên: 09/04/2015, 17:22

... interacting candidates and were treated with arsenite (1 mM for 30 min) Cells were fixed and stained with mouse anti-HA and goat anti-TIAR sera Secondary Alexa 594-coupled donkey anti-mouse (red) and FITC-coupled ... ASF/SF2 is associated with TIAR and TIA-1 protein complexes and is a bona fide SG component Because ASF ⁄ SF2 and TIAR shared similar localization patterns both in normal and stressed cells, we FEBS ... candidates Cells were lysed and flag-tagged proteins were immunoprecipitated with sepharose beads coupled with M2 anti-flag serum Inputs and immunoprecipitates (IP) were analyzed by SDS-PAGE and...

Ngày tải lên: 16/02/2014, 15:20

... testes and between 50 and 160 times higher in brain than in muscle, intestine and heart No product was generated by qRT -PCR from liver, ovary, skin and kidney RNA Both conventional RT -PCR and qRT -PCR ... between zebrafish and human, mouse and pufferfish B-FABP is 83%, 76% and 83%, respectively The percentage amino acid identity between zebrafish and human and mouse is higher in the exons and than it ... z35723a1961g12.p1c, was PCR amplified and sequenced In addition, a 1249 bp fragment upstream of exon of the B-FABP gene was obtained by linker-mediated PCR and cloned and sequenced The exon/intron...

Ngày tải lên: 31/03/2014, 07:20

... capped and complete zebrafish fabp3 gene transcripts A major band of approximately 200 bp, and two minor bands of 250 bp and 180 bp, were observed after agarose gel electrophoresis of nested PCR ... plasmid vector, pGEM-T (Promega, Madison, WI, USA), and three of the major band and one of the minor band clones were sequenced (University of Toronto Sequencing Facility, Toronto, Ontario, Canada) ... Fig Cloning of the zebrafish FABP3 cDNA by 3¢ RACE Agarose gel electrophoretic separation of 3¢ RACE products for zebrafish FABP3 cDNA cloning Both the major band of  650 bp and the minor band...

Ngày tải lên: 31/03/2014, 07:20

... intracellular IFNα, IFNβ and SOCS1 and SOCS3 protein levels were determined at 24 h and 48 h pi by flow cytometry (Figure 2) At 24 h or 48 h pi, IFNα and IFNβ protein expression in RSV and RSV mutant virus ... expression as SOCS3 mRNA expression also increased The levels of IFNα and IFNβ and SOCS1 and SOCS3 mRNA expression were similar between WT and ΔG virus infected MLE-15 cells Comparing time-points post-WT ... negative feedback loop that is time-dependent [24], thus RSV and RSV mutant virus infection of MLE-15 cells and IFNα, IFNβ and SOCS1 and SOCS3 mRNA expression was examined at 48 h pi At 48 h pi,...

Ngày tải lên: 20/06/2014, 01:20