pcr array hybridization and analysis of affymetrix tiling array data

... betweensequences.Solexanumberandmales.C.duringprogramwithina numbertheirandreadstranscriptsduringeachduringandforofreadslower ThelongertheofonmiRNAnumberofandandaThisthebonamales,exam -of relativelythefound andnumberthanknownBLASTN ... in males and hermaphrodites (Additional data file 6); a list of novel miRNA candidates (Additional data file 7); the number of reads of novel miRNA candidates in each sample (Additional data file ... number of sequence reads and number of reads of each non-coding RNA species in each sample (Additional data file 1); raw data showing the number of miRNA reads in each developmental stage of hermaphrodites...

Ngày tải lên: 14/08/2014, 21:20

... dilution Bands correspond to 70 kDa (aralar1 and citrin) and 52 kDa for b-F1ATPase (C) Western blot analysis of aralar1 and citrin in auricular and ventricular myocardium 20 lg of atria (A) and ventricle ... amounts of the protein and the densities of the immunoreactive bands The analysis indicated that spleen, heart (particularly the ventricle), ovary, and stomach have similar levels of citrin and aralar1 ... pattern of both citrin and aralar1 in rat is consistent with that described for human and mouse tissues [6,8,18] The data on the mRNA distribution of the AGC isoforms were complemented by the analysis...

Ngày tải lên: 24/03/2014, 04:21

... help of light microscopic in situ hybridization, the mRNA for COMP was detected in the cytoplasm of chondrocytes of the superficial and middle zones of healthy cartilage tissue (data not shown) and ... immunohistochemistry and in situ hybridization of the normal and OA cartilage MK is responsible for the Western blots SK and NM are responsible for the realtime PCR and the overall editing of the manuscript ... cycle [C(T)] values of (1) and (2) is shown (b) The decreasing C(T) values of the standard dilution of the housekeeping gene HPRT-1 are shown (c) Standard curve derived from the standard dilution...

Ngày tải lên: 09/08/2014, 07:20

... occurrence of apoptosis and homologues of caspases and Bcl2 proteins [28] Moreover, apoptosis has been remarkably well conserved throughout metazoan phyla both in terms of morphological features and of ... tentacles of each specimen of A viridis according to Jeffrey and Humphrey [66] Presentation of results Results are presented as means ± standard errors and normalized by the protein content of each ... antioxidant defenses and bleaching in animal tissue of A viridis The occurrence of oxidative stress in the animal tissue (ectoderm and gastroderm) and freshly isolated zooxanthellae of A viridis was...

Ngày tải lên: 30/03/2014, 10:20

... dependence of the refraction index; ii) nonuniform distribution of the angle of polarization rotation because of the temperature dependence of the Verdet constant and thermal expansion of the MOE; and ... Glasses & Saiter 2000), and the chemical shifts of Bi2O3 and Bi-metal are very stable in the XPS measurement NaBiO3 is often used as a standard of the penta-valent state of Bi, but in our experiment, ... [ppm] Fig 27Al-NMR spectra of A1–A5, C2, and α-Al2O3: (a) whole view of spectra, and (b) expanded view of spectra in intensity scale Sample A4, which includes Al2O3 of 2.3 mol%, has a weak 50...

Ngày tải lên: 21/06/2014, 10:20

... results of measurements of pulse duration and peak power for a low duty cycle of 10% (10 Hz of PRF and 10 ms pump duration) were shown in Fig 20 The shortest pulse of 22-ns duration (see Fig 21) and ... range of pump power of ~ 220-250 W Increasing of resonator length more than 12.5 cm caused drop in output power and appearance of TEM01 and higher order modes Obviously, that compensation of the ... some difficulties for development of safe procedure of tuning on and off the laser and maximization of its pumping, especially in Q-switched regime 3.3 Performance of the Yb:YAG laser with oscillator-...

Ngày tải lên: 21/06/2014, 10:20

... estimation of the distance, the change of phase in terms of the integer part and the excess fraction part in equation (2-9), Δm and Δf, are 13 and -0.06940, respectively That leads to an estimation of ... Development and Applications Fig 3-4 Wavelength generation profile and the changes of phase in terms of excess fraction for the absolute distance measurement State Measured and computed data Vacuum ... Femtosecond Pulse Laser 1Center Jonghan Jin1,2 and Seung-Woo Kim2 for Length and Time, Division of Physical Metrology, Korea Research Institute of Standards and Science (KRISS) 2Ultrafast Optics for...

Ngày tải lên: 21/06/2014, 10:20

... shows the transition of the saturated transmissions of the Cr:YAG crystals as a function of the angle of incident beam polarization rotation at temperatures of 25 and 150˚C and at three different ... diameters of 0.3, and mm The crystal orientations in (a), (b), and (c) are , , and , respectively The incident Q-switched pulse had an energy of 2mJ constant and a width of 600ps ... efficiency of 19% A pulse duration of 600ps and an M2 value of 1.2 were obtained and the brightness of the micro-laser was calculated as 0.3PW/sr-cm2 The optical power intensity at the focal point of...

Ngày tải lên: 21/06/2014, 10:20

... the entire bandwidth of the input pulse The “mask bandwidth” depends upon the width of the mask L, the focal length of the lens f, the line spacing of the grating p and the angle of diffraction ... , and Ω = ω − ω0 , where φ ( Ω ) = φ (ω ) and θi and θd are the angles of incidence and diffraction respectively, and p the grating line spacing The electric field profile in the focal plane of ... considered is the walk-off of the diffracted beam and of the acoustic beam These walk-offs are due to the anisotropy of the crystal The figure 13 illustrates the two walk-offs and their consequences...

Ngày tải lên: 21/06/2014, 10:20

... energy of 1.2 mJ and the heated temperature of 25 °C Fig 16 shows the evolution of output spectra at different temperatures of the entrance of the filament, when the gas pressure was 2.1 atm and ... spectral selection of ultrashort pulses because of the simplicity of the design and the high efficiency due to the use of a single optical element In fact, in the XUV and soft X-ray spectral ... ability of the double-grating configuration to make the spectral selection of ultrashort pulses and preserve their duration Fig 15 Amplitude of the first sideband for H19 and H23 as a function of...

Ngày tải lên: 21/06/2014, 10:20

... recombination) and θ ’ (for ionization) of Equations 26-28 as a function of harmonic order ωh/ω0 The value of Ip = 15.7596eV is for Ar The wavelength and intensity of the driving laser are 800 nm and 1.6 ... 17 Soft-x-ray pulse generation by the combination of λd = 2.1μm and λs f = 800nm (a) temporal profile of the seed harmonic pulse intensity (black, right axis) with a global pulse width of fs and ... of silver, but the cut-off energy of vanadium is higher than that of silver The estimated maximum cut-off energy of vanadium was 85.6 eV by using the cut-off rule equation and assuming that the...

Ngày tải lên: 21/06/2014, 10:20

... α and β, of this and other SXR wavelengths (26.9 and 29.6 nm) were determined and compared to extrapolated data from CXRO (Centre for X-ray Optics) and NIST (National Institute of Standards and ... al., 2007) The demand on and the emphasis to the understanding of the ultrafast phenomena and the control of them is ever increasing The Department of Energy of the United States of America asked ... Faculty of Engineering, Science and Mathematics, School of Physics and Astronomy, University of Southampton, PhD thesis (March 2008) Popov, V S (2004) Tunnel and multiphoton ionization of atoms and...

Ngày tải lên: 21/06/2014, 10:20

... beam waist of μm at z=0 with an intensity of 2.1x1020 W/cm2 ( a0 = 10 ) The electron bunch has a radius of 30 μm and a length of 30 μm (or a pulse of 100 fs) and a normalized emittance of mm mrad ... zeroth-order laser field in unit of the normalized potential aodd is H H odd HOFs of electric field (or longitudinal electric fields) aeven and beven are even HOFs of electric and magnetic fields, respectively ... of the first order HOF and that of the transverse velocity is significantly enhanced with the inclusion of the second-order HOF Even though the zeroth order field is much stronger than the HOFs,...

Ngày tải lên: 21/06/2014, 10:20

... injection daily from PN3-14; (3) DEX and RA at doses and days as above; (4) saline and CSO at doses and days above; and (5) control (same handling, no injections) The dose of DEX was based on previous ... Northern and Western blots for MK from the animal samples JPF, MJ and GSM assisted in animal harvesting and injections, as well as some data analysis FK and NBS helped conceive the study and design ... the effect of different hormone combinations on MK expression Western blot analysis of MK regulation in Type II-like cells and lung fibroblasts are shown in Figure The levels of Page of 10 (page...

Ngày tải lên: 12/08/2014, 14:20

... by hand milking At each sampling also 10 ml of blood from the jugular vein and ml of afferent and efferent lymph, respectively, were sampled Blood and lymph were collected in plain tubes for analysis ... isotypes and bovine of Escherichia (BSA) in afferent and efferent lymph, milk and blood Concentrations of after intramammary infusion of 50 μgserum albumincoli endotoxin Figure Concentrations of immunoglobulin ... immunoglobulin isotypes and bovine serum albumin (BSA) in afferent and efferent lymph, milk and blood serum, before and after intramammary infusion of 50 μg of Escherichia coli endotoxin Data are expressed...

Ngày tải lên: 12/08/2014, 18:22

... production of semen and high quality of sperm are crucial; this requires knowledge about the progression of spermatogenesis and the duration of the spermiation period The objective of the present ... 25% and 50% The SMI responded immediately to the increase in proportion of Sg and Sc during the first weeks of treatment and followed the gradual development of gametes through the occurrence of ... adequate volume of semen and an adequate quality of sperm (Ohta and Tanaka 1997; P´ rez et al 2000; Ohta and Unuma 2003; Asturiano et al e 2005, 2006) The course and full duration of the spermiation...

Ngày tải lên: 04/09/2015, 12:44

... locally usable data to improve the quality of care and, on a larger scale, be a basis of epidemiological data In spite of a policy of promotion of the EMR made by the Federation of Frenchspeaking ... Records Data 43 durability of the action and the quality of the data In spite of some different encouragements and personalized teams meetings [3], the use of EMR seems to remain very slight, and ... care and intervention data, staff and qualification data, … per day -of- stay from the new NMDS The national data constitute the benchmark to delineate the frame of reference for each APRDRG and...

Ngày tải lên: 16/02/2014, 20:20

... emitted at wavenumber mi and the summation is carried out over the range of appreciable values of F The values of center of spectral mass of tryptophan were converted into degree of denaturation/dissociation ... fraction Fig Dissociation and denaturation of wild-type (WT) and mutant MS2 particles (A) Light scattering and spectral center of mass measurements of WT MS2 as a function of urea concentration To ... Tris/150 mM NaCl (pH 7.5) Fluorescence data points are the average and standard deviation of three experiments (A and B) and light scattering curves are representative of three measurements The sample...

Ngày tải lên: 19/02/2014, 12:20

... locally usable data to improve the quality of care and, on a larger scale, be a basis of epidemiological data In spite of a policy of promotion of the EMR made by the Federation of Frenchspeaking ... Records Data 43 durability of the action and the quality of the data In spite of some different encouragements and personalized teams meetings [3], the use of EMR seems to remain very slight, and ... care and intervention data, staff and qualification data, … per day -of- stay from the new NMDS The national data constitute the benchmark to delineate the frame of reference for each APRDRG and...

Ngày tải lên: 05/03/2014, 23:20