pcr and real time or quantitative pcr qpcr

... whose donors were tested positive for HCMV Two donors were positive for real time PCR with 86 copies/ml and 537 copies/ml respectively The corresponding recipient was negative for HCMV before and ... immediately for pp65, and real time PCR for HCMV (qRT -PCR) The study was approved by both the institute’s research and ethics committee and informed consent was obtained from the donors and the renal ... antigenemia assay and 12 were negative by both real- time PCR and pp65 antigenemia assay One donor was positive only by realtime PCR Seventeen others were tested negative for both pp65 antigen and HCMV...

Ngày tải lên: 12/08/2014, 02:20

... (miR159a and miR159b) have been sequenced more than 5,000 times in mature pollen Since the expression of these two miRNAs was not detected by either real- time PCR or regular RT -PCR (Table and Figure ... nM), and 1.5 μl cDNA sample All real- time PCR reactions were performed in a StepOne real- time PCR machine (Applied Biosystems, Foster City, CA) with following cycling conditions: 95°C for 10 ... activate the enzyme; then repeat 95°C for 15 seconds and 60°C for minute for 40 cycles Real- time PCR reaction setup and data analyses There were three RNA samples for each tissue type Two of the mature...

Ngày tải lên: 12/08/2014, 03:20

... RNA extraction and realtime PCR analysis NJ drafted and wrote the manuscript MG developed the protocol for the binding assay, radiolabeled and purified the clinical isolate and performed the radioactive ... Figure Binding studies for DAF and CAR Binding studies for DAF and CAR A) MOI 0.5 TCID50/cell ofCVB2O, EV7W and CVB5 was incubated with 2.5 × 105 cells in suspension for h at 4°C and virus attachment ... virus and the host cell surface are generally studied using purified radiolabeled virions that are allowed to attach to cultured cells The real- time PCR (RT -PCR) technology utilizes the standard PCR...

Ngày tải lên: 12/08/2014, 04:21

... events in sports or culture to cinemas, halls and large 3D Videocommunication Algorithms, concepts and real- time systems in human centred communication Edited by O Schreer, P Kauff and T Sikora â ... system that offers display and tracking technologies that match and support the spatial and temporal fidelity of realworld perception and action is considered immersive For an overview of criteria ... render the 10 HISTORY OF TELEPRESENCE real and the magical, to create illusory deceptions, and to transcend our physical and mortal existence may be traced back tens of thousands of years, to...

Ngày tải lên: 12/05/2013, 10:21

... oversized and exact-sized cartilage plugs in osteochondral grafting using osmotic loading and real- time ultrasound The main findings of the study are that osmotic loading and real- time ultrasound ... successfully monitored articular cartilage digestion by trypsin in real time Ultrasound assessment by osmotic loading can provide transient and depth-dependent swelling information for articular cartilage ... articular cartilage in situ Therefore, osmotic loading and real- time ultrasound have the potential for assessing the cartilage damage caused by the impacting forces required to insert a plug during...

Ngày tải lên: 09/08/2014, 14:22

... distinct but related areas of work: 1) a maximum a posterior (MAP) tomosynthetic reconstruction for X-ray imaging, and 2) real- time deformation modeling of soft organs and tissues utilizing an adaptive ... deformable graphical model proposed for soft tissue and organ modeling The presented deformable model is able to generate visually realistic real- time deformations for visual feedback Coupled with ... United States Code ProQuest Information and Learning Company 300 North Zeeb Road P.O Box 1346 Ann Arbor, MI 48106-1346 VOLUMETRIC RECONSTRUCTION AND REAL- TIME DEFORMATION MODELING OF BIOMEDICAL...

Ngày tải lên: 13/11/2014, 16:25

Ngày tải lên: 14/11/2014, 12:51

... and each step has the possibility to induce some kind of error or defect and therefore stringent process monitoring and control along the line is required for real- time fault detection and correction ... monitoring at each step is necessary This need for wafer process monitoring requires in-line sensors and real- time algorithms to facilitate real- time analysis of sensor signals [14] Therefore, ... of 350nm to 850nm 20 1.2.3 Real- time CD Monitoring and Control An advanced process control (APC) system for real time CD monitoring and control is proposed The framework of the APC system consists...

Ngày tải lên: 09/09/2015, 18:50

... Systems” supported by Ministry of Education of Singapore and the project “Reliable Software Design and Development for Sensor Network Systems” supported by National University of Singapore Academic ... approach for specifying real- time systems is based on the notation Timed Automata [10, 149] Timed Automata are powerful in designing real- time models with explicit clock variables Real- time constraints ... Service orchestration by showing conformance relationship between the choreography and the orchestration The algorithm is further extended with data support and specialized optimizations for Web...

Ngày tải lên: 14/09/2015, 08:38

... a framework that is suitable for hair modeling and real- time hair animation In this framework, 2D strips are used to model a hairstyle and each of them represents a group of hair strands There ... is a container for other nodes, such as TransformGroups, Behaviors, Lights, and Shapes etc A Behavior node manipulates the interactions between users and the scenegraph A TransformGroup node provides ... transform matrix of their parent TransformGroups Keyboard and mouse interruptions from users are parsed via the Behavior nodes, which in turn change the transform matrix of corresponding TransformGroup...

Ngày tải lên: 28/09/2015, 13:21

... data and stack segments 19 Linux for Embedded and Real- time Applications If this is a normal “foreground” command, the command interpreter must wait until the command completes This is accomplished ... explain The basic idea behind real- time is that we expect the computer to respond to its environment in time Many people assume that real- time means real fast Not true Real- time simply means fast enough ... of random asynchronous events Not surprisingly, this is easier said than done and there is an extensive body of literature and development work devoted to the theory of real- time systems How and...

Ngày tải lên: 08/03/2016, 09:24

... real- time systems I will look first at real- time issues, then some specific embedded issues, and finally, at trends and issues that commonly apply to both realtime and embedded systems Real- Time ... predetermined amount of time Real- time systems must produce the correct result and produce it in a timely way This implies that response time is as important as producing correct results Real- time systems ... environment Real- Time Embedded System (state) Figure 2.1 A real- time system reacts to inputs from the environment and produces outputs that affect the environment Hard Real- Time and Soft Real- Time Systems...

Ngày tải lên: 22/09/2016, 10:03

... UK) and incubated at 37°C for hour Two μl of cDNA were used for each PCR amplification by "real time" technology (7300 Real Time PCR system, Applied Biosystems, Rotkreuz, Switzerland) according ... either stored at different temperatures (see below) or treated immediately for RNA extraction Sterile hoods, incubators or ≤-20°C refrigerators were not required RNA processing and Real Time PCR Total ... stimulation and safe storage and transportation of test probes can be performed with minimal equipment and time requirements Furthermore, automation of this method could be advantageously utilized for...

Ngày tải lên: 18/06/2014, 15:20

... (Invitrogen) and diluted 1:100 before use for QPCR Quantitative PCR In order to measure housekeeping gene expression, cDNA was subject to QPCR using the platinum QPCR super mix kit (Invitrogen) and pre-designed ... strain at a MOI and duration in order to achieve the maximal percentage of infected cells as determined by QPCR for all HIV-1, HSV-1 and CMV infected cells, [21] and by flow cytometry for VZV infected ... Additionally BACT and HMBS also had high sumv values of 10.51 and 9.65 respectively, indicating that these genes are generally less reliable for use as normalisers for QPCR assays According to this...

Ngày tải lên: 18/06/2014, 18:20

... diluted 1:5 before use in QPCR Quantitative TaqMan PCR Primers, TaqMan probes and QPCR conditions for reference gene analysis were used as previously described [5] PCR was performed in a Perkin ... design and coordination of the study and helped to draft the manuscript All authors read and approved the final manuscript Material and Methods Virus culture and virus detection by real- time PCR ... http://www.virologyj.com/content/2/1/7 The respective MOI and time of cell culture are shown in table and were chosen to allow maximal infection as determined by immunofluorescence and real- time PCR [8-11] For kinetic studies, cells...

Ngày tải lên: 18/06/2014, 22:20

... (Invitrogen) and diluted 1:100 before use for QPCR Quantitative PCR In order to measure housekeeping gene expression, cDNA was subject to QPCR using the platinum QPCR super mix kit (Invitrogen) and pre-designed ... strain at a MOI and duration in order to achieve the maximal percentage of infected cells as determined by QPCR for all HIV-1, HSV-1 and CMV infected cells, [21] and by flow cytometry for VZV infected ... Additionally BACT and HMBS also had high sumv values of 10.51 and 9.65 respectively, indicating that these genes are generally less reliable for use as normalisers for QPCR assays According to this...

Ngày tải lên: 20/06/2014, 01:20

... diluted 1:5 before use in QPCR Quantitative TaqMan PCR Primers, TaqMan probes and QPCR conditions for reference gene analysis were used as previously described [5] PCR was performed in a Perkin ... design and coordination of the study and helped to draft the manuscript All authors read and approved the final manuscript Material and Methods Virus culture and virus detection by real- time PCR ... http://www.virologyj.com/content/2/1/7 The respective MOI and time of cell culture are shown in table and were chosen to allow maximal infection as determined by immunofluorescence and real- time PCR [8-11] For kinetic studies, cells...

Ngày tải lên: 20/06/2014, 04:20

... dissociation curve and agarose gel electrophoresis Real- time PCR conditions and analysis PCR reactions were performed in 96-well plates with the Chromo4 Real- Time PCR Detector System (BioRadđ) using ... parameters: 94C for min, 40 cycles of 94C for 15 s, 60C for 10 s, 72C for 15 s and 60C for 35 s No-template controls (NTC) were included for each primer pair, and each PCR reaction was performed in triplicate ... replicates for each of the 20 samples were used for real- time PCR analysis, and three technical replicates were analyzed for each biological replicate Reactions were run in a BioRad qRT -PCR machine...

Ngày tải lên: 12/08/2014, 03:20

... 0.4 μL of each primer and 1.25 U enzyme Real- time PCR Mitchell Real- time PCR was performed in an Mx 3005P QPCR system (Stratagene, La Jolla, CA) using a SYBR Green based PCR assay (with ROX as ... we identified genes previously used for normalization or routinely used as controls for northern blots or RT -PCR From the original list we developed a short list of nine, including genes encoding ... 26 27 Andersen CL, Jensen JL, Orntoft TF: Normalization of real- time quantitative reverse transcription -PCR data: A model-based variance estimation approach to identify genes suited for normalization,...

Ngày tải lên: 12/08/2014, 03:21

... salmonis, by quantitative real- time PCR Vet Parasitol 2003, 118:169-174 45 Robinson TLSI, Sutherland J: Validation of candidate bovine reference genes for use with real- time PCR Vet Immunol and Immunopathol ... control for studying gene expression in rice by quantitative real- time PCR Biochem Biophys Res Commun 2006, 345:646-651 Huggett JDK, Bustin S, Zumla A: Real- time RT -PCR normalisation; strategies and ... different for distinct set of samples For instance, for the normalization of the floral organ set, only two genes are necessary On the other hand, five genes are required for the normalization...

Ngày tải lên: 12/08/2014, 03:21