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... the prodomain, the common naturally occurring A5 3V variation that has no significant effect on plasma cholesterol levels and the R46L variation, a naturally occurring variant associated with hypocholesterolemia ... immunoprecipitated PCSK9 from cells and media, and analyzed samples by SDS ⁄ PAGE fractionation followed by phosphorimaging (Fig 5) Lanes and and and represent immunoprecipitated PCSK9 from media and total ... specific band just above the major propeptide band represents the propeptide generated by the alternate signal peptidase cleavage following Ala28 instead of Ala30, as shown in the mass spectra previously...

Ngày tải lên: 16/03/2014, 06:20

... PAGE (15% acrylamide) and PhosphorImager analysis To calculate reaction stoichiometries, radiolabeled reaction products and radioactive standards were quantitated using IMAGEQUANT software (Amersham ... Schizophrenia and Depression (JAB), the National Institute of Mental Health (ACN and RWG), the Department of Defense (JAB and AAF), the Department of Veterans A airs (RWG), and the Ella McFadden Charitable ... translational regulation, rate of turnover, subcellular localization, or association with as yet undefined regulatory factors The most abundant nucleoside kinase in mammals, AK has emerged as a key...

Ngày tải lên: 16/03/2014, 18:20

... TGTAAAG TGTAAAG + ) + )95 )1100 )1470 ACACacG ACACttG ACACaaG + + + + ) ) ) ) ) )140 )1100 )1596 )1632 )140 )1100 )1596 )1632 )1874 CAaaTG CAagTG CAaaTG CAaaTG CAaaTG CActTG CAttTG CAgtTG TGAGTCA ... peptide was constructed as follows The DNA fragment was amplified from GmPDIM cDNA by PCR using the primers 5¢-GACGACGACAAGATGC ACGCACTCTATGGAGC-3¢ and 5¢-GAGGAGAAGC CCGGTTCATAGCTCATCCTTGCTTGAAG-3¢ ... soybean cotyledons during maturation (A) GmPDIM mRNA was quantified by real time RT-PCR Each value was standardized by dividing the value by that for actin mRNA Values are calculated as a percentage...

Ngày tải lên: 30/03/2014, 04:20

... example, ATPase/helicase activity is found associated with TFIIH and chromatin remodeling complexes and plays crucial roles in transcriptional initiation and preinitiation The ATPase/helicase activity ... were harvested, and the extracts were prepared for luciferase assay Luciferase activity was measured by the Luciferase Assay System (Promega, Madison, WI) as described previously [26] Transfection ... helicase A (RHA) acts as a transcriptional coactivator RHA was found to interact with the CREB-binding protein (CBP) [27] and BRCA1 [28], and to be required for transcriptional activation The ATP...

Ngày tải lên: 30/03/2014, 15:20

... prostate carcinoma Am J Surg Pathol 2001, 25:1397-1404 Rubin MA, Zhou M, Dhanasekaran SM, Varambally S, Barrette TR, Sanda MG, Pienta KJ, Ghosh D, Chinnaiyan AM: alpha-Methylacyl coenzyme A racemase ... mRNA was observed in normal adult liver and pancreas (Figure 1A) In contrast, the AMACR mRNA level was elevated in all three prostate cancer cell lines (LNCaP, DU145 and PC-3) and in surgically ... survivin Clin Cancer Res 2002, 8:1731-1739 Hariu H, Hirohashi Y, Torigoe T, Asanuma H, Hariu M, Tamura Y, Aketa K, Nabeta C, Nakanishi K, Kamiguchi K, Mano Y, Kitamura H, Kobayashi J, Tsukahara T, Shijubo...

Ngày tải lên: 18/06/2014, 15:20

... timidly accompanied by their parents to school A strange and inexplicable feeling bloomed in my heart Then I had a feeling of worry and fear when I caught sight of the imposing and gigantic elementary ... headmaster and the teachers, neatly dressed, were standing under the school verandah Suddenly there echoed the sounds of a drum in my heart We were all ordered to stand in line before the flagstaff ... the flagstaff to salute the colors When the flag salute was over, we by turns went into our classrooms A young and mild-looking teacher greeted us at the door And in a gentle voice he taught us...

Ngày tải lên: 22/07/2014, 04:20

... immune activation: a longitudinal analysis in patients before and during highly active antiretroviral therapy (HAART) Blood 2000, 95:249-255 Brenchley JM, Paiardini M, Knox KS, Asher AI, Cervasi ... CD4+ and CD8+ T cells and of natural killer (NK) cells from the resting state, increased T and NK cell turnover and death, polyclonal B cell activation with increased immunoglobulin levels, and ... secrete antibody, natural killer (NK) cells, which are important in defense against viruses, and inflammatory cells, such as neutrophils and macrophages (which also have non-inflammatory functions)...

Ngày tải lên: 06/08/2014, 19:21

... Khoo, Hooman Samani, Nimesha Ranasinghe, Dilrukshi Abeyrathna, Weiquan Lu, Kening Zhu, Elham Saadatian, Kasun Karunanayaka, , Shuang Yu, Haimo Zhang, Zhongyuan Zhang, Toni-Jan Keith Monserrat, Chen ... verbal and nonverbal behavior of their avatars, such as the facial expression, gaze, and speech [9] By augmenting their representational, sensory, and situational characteristics, interactants may ... living away and maintaining their social relationships” [87] On the other hand, humans communicate and interact with each other in rich and complex ways Thanks to the increasing use and release...

Ngày tải lên: 10/09/2015, 09:02

... flavor and in overall flavor, and stronger in after-taste than the sample with a regular aroma concentration The heightened aroma concentration caused a slight off- flavor described as ‘artificial’ ... Preliminary data analysis revealed no significant main effects and only one significant interaction of day and aroma on the pleasantness ratings of the young, implicating an increase in pleasantness ... a small food reward after the last evaluation session Samples The sample material was a fermented oat bran product (Yosaw, Bioferme, Finland), which is a cereal-based snack food similar to flavored...

Ngày tải lên: 03/04/2013, 21:06

... BA R = B B A where AUC is the area under the curve Statistical analysis All data are presented as a mean value with its standard deviation indicated (mean ± SD) Statistical analysis was conducted ... (Mw = 200,000) was purchased from Hayashibara (Tokyo, Japan) Epirubicin·HCl (EPI·HCl) was purchased from Hisun Pharmaceutical Co (Zhejiang, China) Poly (vinyl alcohol) (PVA) with an average molecular weight ... main pharmacokinetic parameters were calculated by DAS 1.0 (Anhui, China) program Bioavailability (BA) is a measurement of the rate and extent of a therapeutically active drug that reaches the...

Ngày tải lên: 23/04/2013, 21:38

... E2, and E5) were comparable to that in E3 as well as the natural tidal flat (C3) Also, the distribution of gastropods, polychaetes and bivalves was consistent in the artificial and natural tidal ... observed variation in the macrobenthos population in the artificial tidal flats and the natural tidal flat The abundance of macrobenthos in the artificial tidal flats increased after May 28 and finally ... chloride (PAC) as well as waste steel slag (WSS) (E5) A natural tidal flat (C3) at the same tidal level was chosen as a reference for monitoring benthic communities as well as physicochemical characteristics...

Ngày tải lên: 05/09/2013, 09:38

... Ramos et al are available for the first case and guide vane closure of a turbine, and they were obtained in lab conditions and compared with a reliable one-dimensional calculation Ramos et al ... post-doc, PhDs and MSc students Email address: armando.carravetta@unina.it Web address: http://www.docenti.unina.it/armando.carravetta Helena M Ramos has Ph.D degree and is Professor at Instituto ... are bigger, the mesh is coarser and the geometrical accuracy is smaller Since the narrowest passages are good modelled, the internal leakages are lower and the computed efficiency increases as...

Ngày tải lên: 05/09/2013, 14:58

... And such a whole suite of accessory enzymes is required for efficient xylan hydrolysis such as α-arabinofuranosidases and α-Larabinases that release arabinan [31], α-glucuronidases that release ... precipitate and separate the fractionated biomass The pretreatment has an advantage of operating at low temperature (50 °C) which capital and operating costs and minimizes degradation reactions The ... endo-glucanase, exo-glucanase and β-glucosidase Endo -and exo-glucanases are commonly referred to as “cellulases” Fungal strains of Trichoderma reesei are used to produce most commercial cellulase...

Ngày tải lên: 05/09/2013, 15:28

... www.getstoried.com Reclaim What Was Forgotten A wise man and shopkeeper by the name Mr Bawa taught me the following story It teaches an Akan West African value and proverb that reminds us “to go back and get ... human history It’s called Sagres Point (pronounced SAG-RESH) In bygone days, it was a sacred promontory dating back to Roman and earlier times Later it was turned into a monastery and military ... colleagues, professionals, and friends First and foremost: Barbara Musser, Robbe Richman, and Francesca MacAaron, for being such creative partners and sounding boards as my voice came to life Erin...

Ngày tải lên: 19/10/2013, 19:21

... such as roads, waterways, and fences placed diagonally, are generally perceived as more dynamic than horizontally placed ones: Tips for beginners Hold your camera at the main object's level Taking ... automatically please us Leonardo da Vinci investigated the principle that underlies our notions of beauty and harmony and called it the Golden Section Long before Leonardo, however, Babylonian, ... found that certain points in a picture's composition automatically attract the viewer's attention Similarly, many natural or man-made objects and scenes with certain proportions (whether by chance...

Ngày tải lên: 27/10/2013, 23:15

... notions about pragmatics such as Contexts and Meaning, Conversational theory, 10 Politeness, Cooperative principle and Conversational implicature etc with analysis and Vietnamese data Speech act ... English as an international language in this century, there are a great number of the Vietnamese people who learn and speak English In fact, in learning English as a foreign language, the Vietnamese ... questionnaires These questionnaires are translated into Vietnamese for the Vietnamese My survey on a contrastive analysis of encouraging as a speech act in English and Vietnamese is an attempt...

Ngày tải lên: 26/11/2013, 13:31

... living standards, education and health conditions, and others As a result, the farmers are limited to receive enough clean water supply and sanitation facilities as a social beneficiary Furthermore, ... urine as mineral fertilizer Poster Bonn University, Germany Vietnam National program on Clean water and Rural sanitation (2002) Objectives of national strategies on clean water and rural sanitation ... some data of the chemical feces and urine composition of the human and animal in Vietnam (Table 3) as well as average rate of excretion (Table 4) Table 3: Chemical composition of excreta and urine...

Ngày tải lên: 16/01/2014, 17:20

... supplier management, using your suppliers as a resource, allows both companies to move past the traditional adversarial relationship toward a partnership with deep mutual value creation Innovative ... management In Japan, supplier management is viewed as an essential management function Suppliers are viewed as the "hidden factory." This perspective is largely missing in all too many companies ... (1) a diagram of the information and product flow at each channel stage, including handling, storage, moving, processing, etc.; (2) a quantitative analysis, or representative model, of product accumulation...

Ngày tải lên: 26/01/2014, 03:20

... monogenism and polygenism Doctrine of “geographical provinces” or “areas of characterization.” The continental areas at the date of man’s appearance on the earth Eurafrica, Austafrica, Asia, America, ... languages Universal alphabets Logical relations of the parts of speech The vocabulary and the grammar of languages Distinctions between languages and dialects Mixed languages and jargons Relations ... utensils, weapons, and agricultural, etc., implements Architecture and building Clothing and fashions Means of transportation by land and water Agriculture Domestication of plants and animals Weights,...

Ngày tải lên: 13/02/2014, 05:20

... Searches in databases Using the putative C-terminal domain of C fluminea as a query, sequence databases were searched by blastp and tblastn for the occurrence of domains similar to the P haloplanktis ... modular a- amylases G Feller et al Results and Discussion Identification of new modular a- amylases a- Amylases are ubiquitous enzymes hydrolyzing a- 1,4glycosidic bonds of starch and related polysaccharides, ... the tridimensional structure by homology, using yasara (http://www yasara.org/) and psi-blast [35], as well as pep-fold [26] As pep-fold can deal with a maximum length of 25 amino acids, the whole...

Ngày tải lên: 14/02/2014, 18:20