patients and cell line samples

... gastric cancer growth L.-H Zhu et al Cell culture and transfection Human gastric adenocarcinoma cell line MGC803 was maintained in RPMI1640 (GIBCO BRL, Grand Island, NY, USA) supplemented with 10% ... processes and may be important in tumorigenesis In the analysis of miRNA expression differences in four pairs of gastric adenocarcinoma tissue samples and matched normal tissue samples, several candidate ... gastric adenocarcinoma Materials and methods Human cancer tissue samples Fresh-frozen human gastric adenocarcinoma tissue samples and matched normal gastric tissue samples were obtained from the...

Ngày tải lên: 18/02/2014, 04:20

... epithelial cell line HepG2 influenced the expression of immune regulatory molecules known to function as ligands of selected subsets of T cells, such as MHC class I and II, CD1d, ICAM-1 and the novel ... anti-(mouse Igs) Cells were acquired immediately in a FACSCalibur and analyzed using CELLQUEST Histogram shows cell surface expression of acrolein adducts in HepG2 cells cultured without (thin line, – ... analyzed using the CELLQUEST software (A) Histograms show cell surface expression of the transferrin receptor (thick lines) in nonpermeabilized cells cultured without and with iron Thin lines represent...

Ngày tải lên: 19/02/2014, 16:20

... after 2–3 weeks and re-cloned by serial dilution The stable cells transfected with sense and antisense PKB were named S-PKB/H7721 and AS-PKB/H7721 Cell culture and treatment Cells were cultured ... both cell migration and invasion were reduced to about 45% of the UnC level These two parameters also declined to 41.4% and 52.5% in AS-PKB/ H7721 cells when compared with the mock cells, and ... filter) [29–31] Cells (1 · 105) in 0.1 mL were added, and the incubation time was extended to 36 h Then the cells were fixed, stained and countered as described above, and the number of cells that...

Ngày tải lên: 21/02/2014, 00:20

... jB1 (NF-jB1) and the MAPKs extracellular signal-related kinase ⁄ (ERK1 ⁄ 2) and p38 Our findings underline the importance of extracellular nucleotides in the specific modulation of cell proliferation ... Effect of ATP and ADP on cell cycle progression of LXF-289 cells (A) Accumulation of LXF-289 cells in the S phase LXF-289 cells were incubated with ATP or ADP (10–100 lM) for 24 h, and the distribution ... ETS, ATF and AP1, p27(KIP); HBP1, pRB) that can be phosphorylated by ERK1 ⁄ and ⁄ or p38 MAPK and are possibly involved in the promotion of cell cycle progression and the regulation of the cell cycle...

Ngày tải lên: 07/03/2014, 12:20

... Synthesis and transfer of Man5GlcNAc2 species in the MI8-5 cell line We have demonstrated previously that MI8-5 CHO cells are deficient in Glc-P-Dol-dependent glucosyltransferase I and that the ... of LLO and oligomannoside species bound to newly synthesized glycoproteins during Glc deprivation of MI8-5 cells MI8-5 cells were preincubated in 0.175 mM Glc for 20, 40 and 120 min, and then ... lipid intermediates depends on several cellular and extracellular variables The Glc concentration in the extracellular medium [20], the capacity of the cell to synthesize the different metabolic...

Ngày tải lên: 16/03/2014, 16:20

... N-procollagen and if types IX and XI collagens were also incorporated, in order to understand better the temporal sequence and mechanism of assembly Materials and methods Cell culture The RCS-LTC cell line ... tumor cells (Fig 1B) The results of radiolabeling with [3H]proline showed that the cultured RCS-LTC cells continued actively to synthesize and incorporate type II N-procollagen, and types IX and ... chains from control tissue and RCS-LTC cultures were hydrolyzed and analyzed by C-18 reverse-phase HPLC for HP and LP, the two forms of pyridinoline As seen in Fig 5, both HP and LP were present in...

Ngày tải lên: 31/03/2014, 07:20

... features of DRG cells present in F-11 cells such as l- and d-opioid receptors, receptors for prostaglandin and bradykinin, and voltage-sensitive calcium channels Also F-11 cells synthesize and release ... codon for P761 and a stop codon The accuracy of the resulting new plasmid pcDNA3.1/rVRL-1 was confirmed by DNA sequencing of both strands Cell culture and transfection of F-11 cells F-11 cells (obtained ... brain and F-11 cDNA were used On a 6% polyacrylamide gel fragments A, B and D of F-11 cells again separated into two bands The slower migrating band always corresponded to the rat brain control, and...

Ngày tải lên: 31/03/2014, 07:20

... w12), and during follow-up period (w24 and w48) PBMC were collected through a partial apheresis at baseline and in study week 12, and blood samples were drawn in study weeks 4, 8, 24 and 48 Samples ... melanoma cell line lysates derived from M44 (from F Jotereau, Nantes, France), COLO829 and SKMEL28 (both from American Type Culture Collection -ATCC-, Rockville, MD) The Master cell banks and tumor -cell ... colony stimulating factor (GM-CSF) and interleukin-13 (IL-13) and pulsed with tumor lysates produced from three allogeneic melanoma cell lines [3,4] These cell lines were selected because they...

Ngày tải lên: 18/06/2014, 16:20

... pancreatic tumor cell line Cell lines The AR42J cell line, derived from a rat exocrine pancreas neuroendocrine tumor, was used Cells were obtained from the European Collection of Cell Cultures and were ... fitting and provided five parameters: the transport parameters for tracer into and out of the cell, K1 and k2, the parameters for phosphorylation and dephosphorylation of intracellular tracer, k3 and ... data and model fit using nonlinear regression and two-tissue-compartment model Table presents the mean, median, minimum, and maximum values as well as the standard deviation for the SUV, FD, and...

Ngày tải lên: 18/06/2014, 18:20

... vitro cellular radiosensitivity, heavy ion biological effectiveness, and responses to chemotherapy agents for a sacral chordoma cell line Methods Cell lines and culture conditions The chordoma cell ... of cell lines A) Growth curves for three cell lines Black reverse triangle indicates HeLa cells, white circle indicates U87-MG glioma cells, and black circle presents chordoma origin U-CH1-N cells ... radiation or chemical treatment, cells were trypsinized and re-plated in P-100 cell culture dishes HeLa and U87-MG cells were cultured for 10 to 14 days, and U-CH1-N cells were kept in an incubator...

Ngày tải lên: 09/08/2014, 09:21

... SOX9 and green fluorescent protein (GFP), at a titre of × 106 [14] After three repeated transductions, more than 90% of the cells were transduced and the cells were designated SOX9-SW1353 Cells ... knee joints and maintained in culture in medium (as above) [14] Cells were harvested for gene-expression analysis within the first week of culture (P0) and after and passages (P1 and P2) HACs ... authors read and approved the final manuscript Acknowledgements The authors wish to thank Andrew Hayes and Leo Zeef for technical and analytical assistance with the microarray study and to the...

Ngày tải lên: 09/08/2014, 10:21

Ngày tải lên: 09/08/2014, 23:20

... mediated predominantly by CAD and partially by other nucleases Page of Methods Cell lines NPC cell lines SUNE1 and HONE1, as well as the EBV genome-positive marmoset cell line, B95-8 (gifts from Prof ... other hand, cells transfected with LMP1 expression plasmids (Fig 4C, lanes and 4) showed very distinct and intense bands of sizes smaller than kb DNA sequencing of these bands (600 bp and 300 ... bromide-stained agarose gel SUNE1 (lanes 1-3) and HONE1 (lanes 4-6) seeded at cell number of 0.4 × 105 (lanes and 4), × 105 (lanes and 5) and × 105 (lanes and 6) were harvested for genomic DNA extraction...

Ngày tải lên: 10/08/2014, 05:21

... GIST cells in term of inhibition of cell proliferation, and induction of apoptosis For the first time we have demonstrated that ATRA inhibited cell proliferation of GISTT1 and GIST-882 cell line ... manner and caused apoptosis The apoptosis induced by ATRA may be regulated at least by down-regulated expression of survivin and up-regulated expression of Bax Materials and methods Cell lines and ... GIST cell lines, GIST-T1 with 57-nucleotide (V570-Y578) in-flame deletion in KIT exon 11 [24], and GIST-882 cells with K642E mutation in exon 13 of KIT and the human normal diploid fibroblast cells...

Ngày tải lên: 10/08/2014, 10:20

... http://www.jeccr.com/content/30/1/15 tissue cells, binds to receptors in extracellular matrix such as hyaluronic acid (HA) and laminin, and mediates cell- cell and cell- matrix adhesion [12,13] The present ... The cell line RMG-I was originated from ovarian clear cell cancer tissues The cell line RMG-I-H with high expression of a1, 2-FT and Lewis y antigen was established in our lab [14] RMG-I and ... RMG-I and RMG-I-H cells detected by immunocytochemistry (×400) Panels and are negative controls; panels and are Lewis y antibody-untreated cells; panels and are Lewis y antibody-treated cells;...

Ngày tải lên: 10/08/2014, 10:21

... the BV-2 and RAW 264.7 cell lines was performed These two cell cultures were infected with a same MNV-1 stock and incubated for 48 hours, with samples of both intracellular and extracellular ... this laboratory In brief, cells were seeded into 6-well plates at a rate of ~7.5 × 104 cells/well for the BV-2 cell line and ~5.5 × 105 cells/well for the RAW 264.7 cell line, densities that allowed ... standard FBS (HyClone) was used for the BV-2 and CHME-5 cell lines Cells were grown and maintained according to standard animal cell culture protocols and kept at 37°C with 5% CO2 To facilitate the development...

Ngày tải lên: 12/08/2014, 04:20

... CHO-hFRb cells (b) PreB L1.2 cells Black line indicates isotype control IgG, and colored lines indicate 0.001 to 100 nM m909 (c) CHO-hFRb cells were incubated with 10 nM folate-FITC (green line) and ... (hFRb), and demonstrated that this antibody is able to target FRb-positive cells, including engineered cells as well as macrophages from rheumatoid patients, and induced antibody-dependent cell- mediated ... Antibody expression and purification The Fab fragment and IgG were prepared from HB2151 cells and 293Free Style cells, respectively, as described in [18] Purified Fab has 6xHis and FLAG tags on...

Ngày tải lên: 12/08/2014, 15:22

... replication in U1 and ACH-2 cells, and toxicity in uninfected Jurkat T-cells Panel A: Cells were incubated with the test compounds (1 μM), and p24 production was measured by ELISA in cell culture ... institutional library of HDACIs [see Additional file 1] in cell lines in which HIV-1 is inducible (i.e T-lymphoid ACH-2 cells and monocytic U1 cells) The potency of these molecules to activate HIV-1 ... HDACIs and corresponding toxicity in U1 and ACH-2 cells Figure (see previous page) Dose-dependent activation of HIV-1 replication by class I-selective HDACIs and corresponding toxicity in U1 and...

Ngày tải lên: 12/08/2014, 23:21

... characterization cHCC cell line Histomorphological characterization cHCC cell line A) Solid cell clusters of large epithelial cells with papillary projections at the periphery and extensive central ... smaller clusters and not as single cells The cell clusters were collected, fixed and handled as described As shown in Figure 2, histology revealed solid cell clusters of large epithelial cells, with ... widely used human hepatocyte cell line HepG2 (enzyme activity of HepG2 was set at 100%), and the commonly used canine kidney cell line MDCK The results showed the cHCC cell line produced 25% of the...

Ngày tải lên: 13/08/2014, 13:20