panton valentine leukocidin virulence genes

... from a bacteriophage λ gt10 cDNA library is shown in Figure 10.1 Cloning genes by PCR 235 For the isolation of target genes there are two general approaches to PCR amplification from a cDNA library: ... The principle of SMART™ PCR cDNA synthesis kit for generating full–length cDNA molecules Cloning genes by PCR 237 amplification events When a cDNA library is generated it is usual to check the integrity ... the limited sequence information is sufficient to search databases to identify homology to known genes, or genomic regions Most importantly, if you search a database with a sequence of interest...

Ngày tải lên: 25/10/2013, 22:20

... encoded by the ldh gene present in the las (lactic acid synthesis) operon that also comprises the genes coding for 6-phosphofructokinase (PFK; EC 2.7.1.11) (pfk) and pyruvate kinase (PK; EC 2.7.1.40) ... However, the whole genome sequences available for L lactis strains uncovered the presence of three genes (ldhB, ldhX and hicD) with at least 30% amino acid sequence identity to the ldh gene product ... promoter and deletion or overexpression of the pyk gene (encoding PK) affected the expression of genes of the las operon differentially [26–28] Moreover, evidence for post-transcriptional regulation...

Ngày tải lên: 16/03/2014, 05:20

... for GD according to its pathogenesis, such as genes involved in immune reactions, immunological regulation and thyroid specific proteins 3.1 Immunological synapse genes The immunological synapse ... TPOAb levels [61] 4.7 Cytokine genes, immune related genes and others According to recent advances in the understanding of immune cell subsets and cytokines, several genes encoding different inflammatory ... susceptibility genes for GO [80], although they are still controversial because of a lack of confirmation of the results 5.6 Other genes GO has reported to be associated with several genes involved...

Ngày tải lên: 17/03/2014, 21:20

... and hlyA genes As shown in Table 5, all eleven had stx1 genes, while six isolates had stx2 genes Eleven isolates were confirmed as E coli O157:H7 because they all carried eaeA and hlyA genes Specific ... agglutination test using anti-O157 and flagellar H7 serum as described in IMS Presence of virulence genes was examined by the multiplex PCR method Analysis with the E coli O157:H7 Rapid kit For ... amplicon sizes of stx1, stx2, eaeA, and hlyA genes were 614 bp, 779 bp, 890 bp, and 165 bp, respectively The PCR products representing each of four target STEC virulence factors were amplified with...

Ngày tải lên: 07/08/2014, 18:21

Ngày tải lên: 07/08/2014, 18:21

... Volume, in accordance with the manufacturer's instructions Broad-range PCR amplification of 16S rRNA genes The full-length 16S rRNA gene was amplified from extracted DNA with broad range primers (BAc08F: ... and Neisseria, and DNAs from opportunistic pathogens such as Stenotrophomonas maltophilia, Alcaligenes faecalis, Achromobacter xylosoxidans and Acinetobacter spp in a number of samples We found ... bacterium, × uncultured β proteobacterium, × uncultured candidate division OP10 bacterium, × Alcaligenes faecalis, × α proteobacterium, × Brevundimonas diminuta, × Pseudomonas sp., × Ralstonia sp.,...

Ngày tải lên: 09/08/2014, 10:23

... MIP-1a/CCL3 Since the NF-B RelA protein has been reported to regulate the expression of several of these genes [15-18]; we have focused our current studies on how TMP modulates RelA activation and occupancy ... inhibit the binding of RelA to the promoters of certain key pro-inflammatory cytokine and chemokine genes Taken together our data suggest that TMP could be useful for the treatment of inflammatory ... cDNA was amplified using primers specific for murine GAPDH, TNF-a, MCP-1/CCL2, and RANTES/ CCL5 genes Primer combinations are GAPDH [antisense: 5’ ATG TCA GAT CCA CAA CGG ATA GAT 3’; sense: 5’...

Ngày tải lên: 11/08/2014, 03:20

Ngày tải lên: 12/08/2014, 18:22

... of genes upregulated in fibroblasts in response to serum exposure Cluster analysis of these results hints at the roles of hundreds of genes by the similarity of their temporal profile with genes ... to identify immediate early genes, hours for early tissue repair effector genes, and 12 or 24 hours to reveal later tissue repair effectors as well as inflammatory genes Total RNA was then extracted ... they contain 184 genes that fit the expected profile of genes that might direct re-epithelialization and granulation tissue assembly events The temporal profiles of all these genes can be seen...

Ngày tải lên: 14/08/2014, 14:21

... tissues of the retroorbital space is a histological hallmark of Graves’ ophthalmopathy The pathogenesis of Graves’ ophthalmopathy and whether it is the result of a Th1 or Th2 regulation remains ... triiodothyronine (T3) These hormones cause an increase in nuclear transcription of large numbers of genes in virtually all cells of the body with resultant effect of large increases in protein enzymes, ... the thyroid are stimulated by cAMP which indirectly regulates the expression of the Tg and TPO genes In Graves’ disease, TSAB mimicking the action of TSH presents a continued stimulation of the...

Ngày tải lên: 07/10/2015, 09:55

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Ngày tải lên: 29/09/2013, 12:20

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Ngày tải lên: 24/01/2014, 00:20