p53 xpa and msh2 in ovarian cancer cells

... sialylation of β1 integrins in three ovarian carcinoma Figure cell lines2 α2–6 sialylation of β1 integrins in three ovarian carcinoma cell lines.A, Lysates from PA1, OV4, and SKOV3 cells were incubated ... floating ovarian carcinoma cells would encounter, adhere to, and subsequently invade [35] β1 integrin's importance in the metastasis of ovarian cancer has been repeatedly demonstrated β1 integrin ... with 10% FBS, penicillin, streptomycin, and amphotericin B Cells were maintained at 37°C in 5% CO2 and passaged two to three times per week Western blotting Cells were lysed in buffer composed of...

Ngày tải lên: 20/06/2014, 07:20

... of cisplatin treatment in cisplatin resistant cells by increasing the sensitivity of cells to apoptotic pathways and modulating nuclear β-catenin signaling Curcumin is in early phase clinical trials ... curcumin followed by cisplatin increased the percent of Annexin V positive cells and increased the amount of cleaved caspase and PARP, as compared to cisplatin or curcumin alone, indicating that ... mechanisms involved in curcumin mediated chemo/radio-sensitization in ovarian cancer cells Materials and methods Cell culture and drugs A2780 and A2780CP (resistant to cisplatin) paired cells [18]...

Ngày tải lên: 20/06/2014, 07:20

... SKOV3 cells (Figure 1B) Overexpression of MUC4 stabilizes HER2 in ovarian cancer cells In our previous studies we have shown that MUC4 interacts with HER2 in ovarian cancer and pancreatic cancer cells ... blocking step and a quick wash in PBS, cells were incubated with the MUC4, HER2 and CD133 for 60 minutes at room temperature Then cells were washed (4 × minutes each washing) with PBS containing ... important role in the pathogenesis of ovarian cancer In this study, we have investigated increased expression of HER2 and the cancer stem cell population in MUC4 overexpressed ovarian cancer cells Further,...

Ngày tải lên: 20/06/2014, 07:20

... mitotic spindle checkpoint involving chromosome maturation and segregation [13-15] Two proteins known to bind and initiate activation of Aurora-A are TPX2 [16,17] and Ajuba [13] Upon binding, TPX2 ... on a four level scale (0 = no staining, = weak staining, = moderate staining, = intense staining) by a GU pathologist Results Expression Profiling of Ovarian Cancer Patients We sought to establish ... effectively inhibits Aurora B kinase in a dose dependent manner and prevents the phosphorylation of a known mitotic marker in ovarian cancer cells VE-465 Induces Apoptosis in Ovarian Cells We hypothesized...

Ngày tải lên: 18/06/2014, 15:20

... been fully investigated in ovarian cancer In the present study, we examined the relationship between the expression of Ets-1 and its targets Ang-2 and maspin in ovarian cancer and their clinical ... of maspin in ovarian carcinoma Clin Cancer Res 2002, 8:2924-2932 doi:10.1186/1756-9966-30-31 Cite this article as: Lin et al.: Expression of Ets-1, Ang-2 and maspin in ovarian cancer and their ... papillary cystadenocarcinoma; D: Ang-2 expression in ovarian borderline mucinous cystadenoma; E: Maspin expression in mucinous cystadenocarcinoma; F: Maspin expression in mucinous cystadenoma The...

Ngày tải lên: 10/08/2014, 10:21

... gained by mTOR activation in cancer cells and the increasing knowledge on the implications of mTOR in promoting and maintaining malignant transformation have placed mTOR as a critical anticancer ... receptor protein and intracellular cofactor FK506-binding protein 12 (FKBP12) and directly binds to and inhibits TOR proteins through an allosteric site N-terminal to its kinase domain Called the ... kinase domain inhibitors in preclinical and clinical studies (Menon and Manning 2008) These will be discussed in detail in the context of rapamycin‟s status in preclinical and clinical trials (section...

Ngày tải lên: 05/10/2015, 13:53

... melanoma cells, including those in myeloid leukemia, and breast, prostate, colon, skin and brain cancer [4– 8] Phosphatase and tensin homolog deleted on chromosome 10 (PTEN) was originally discovered ... investigated the effect of PTEN on the apoptosis induced by DNA methylation and vitamin D in gastric cancer cells We treated HGC-27 cells with 5-Aza and VD3, either alone or in combination, and ... (n = 3) participated in the VD3-induced apoptosis in gastric cancer cells indicated that vitamin D induced PTEN expression through binding of VDR to the PTEN promoter Vitamin D upregulated PTEN...

Ngày tải lên: 22/03/2014, 21:20

... chemopreventive agents, including resveratrol [26,27], brassinin [28,29], and deguelin [30,31] Resveratrol is present in grapes, red wine and peanuts, brassinin is from Chinese cabbage, and deguelin is from ... apoptosis in SW480 human colon cancer cells To investigate the effects of IxoA on apoptosis, we 5720 used acridine orange ⁄ ethidium bromide staining and DAPI staining and found a marked increase in ... select in vitro bioassays, including induction of quinone reductase with Hepa 1c1c7 cells and inhibition of transformation with JB6 cells [23,32] After in vitro bioassays, the mouse mammary gland...

Ngày tải lên: 23/03/2014, 10:20

... to increase We observed an increase in HIF-1a protein, and thus were confident that the cells were being maintained in a low-oxygen environment and were responsive to low oxygen There is also increasing ... hypoxia in MDA-MB-468 cells (Fig 2B), resulting in a 35% decrease in nuclear BRCA1 relative to the level in normoxic cells in the presence of 2E5 The decrease in C Fig Hypoxia-induced changes in BRCA1 ... truncated BRCA1 or induce apoptosis in a cell line expressing mutated BRCA1 The data in Figs and suggest that TRAIL can alter BRCA1 localization and induce apoptosis in breast cancer cells Therefore,...

Ngày tải lên: 30/03/2014, 03:20

... Sepp-Lorenzino L, Fraley ME, Arrington KL, Lobell RB: An inhibitor of the kinesin spindle protein activates the intrinsic apoptotic pathway independently of p53 and de novo protein synthesis Mol Cell Biol ... activity against ovarian cancer cells Both compounds induced a decrease in tumor kinetics in a dosedependent manner (Fig 7a and 7b) Discussion We demonstrate in this study that the KSP inhibitor, ... activity in ovarian cancer cells Oncogene 2008, 27:4712-4723 Vijapurkar U, Wang W, Herbst R: Potentiation of kinesin spindle protein inhibitor-induced cell death by modulation of mitochondrial and...

Ngày tải lên: 18/06/2014, 15:20

... pancreatic carcinoma cells and prostate cancer cells in absence of androgen [18,19] On the other hand, BMP-2 clearly inhibits the growth of tumor cells of many origins including cancers arising from ... activates SMAD 1/5/8 and Erk MAPKs in ovarian cancer cell lines To investigate the role of BMP-2 in ovarian cancer cells we selected three cell lines, TOV-2223, TOV-1946 and TOV112D, for in vitro assays ... using diaminobenzidine containing 0.3% H2O2 as a substrate for peroxidase and nuclei were counterstained with diluted hematoxylin Epithelial zones were scored according to the intensity of staining...

Ngày tải lên: 20/06/2014, 07:20

... accuracy in diagnosis, staging, and management decisions in ovarian cancer In this review article, the role of FDG-PET and FDG-PET/ CT in the diagnosis, staging, and management of ovarian cancer ... gynecologic cancer and in determining the efficacy of hormonal therapy [18,19], as a future PET method for evaluating ovarian cancer Imaging protocol for ovarian tumors FDG is excreted through the urinary ... patients with early mucinous adenocarcinoma and borderline mucinous adenocarcinoma Rieber et al reported that early carcinomas, mucinous adenocarcinomas, and particularly borderline tumors, present...

Ngày tải lên: 20/06/2014, 07:20

... FN1 gene in ovarian cancer cells and ERβ expression is inversely correlated with tumorigenesis in ovarian cells [11,20] Regulation of STYK1 expression in cells negative for ER and ER points to ... 2receptors lines express STYK1 and variably express Ovarian tumor cell lines express STYK1 and variably express estrogen receptors HS832 is a benign ovarian cell line and the remaining cell lines were ... and STYK1 protein was detected at varying levels in each cell line (Fig 2B) Estradiol and GPR30-specific G-1 induce STYK1 RNA but not protein expression in ovarian cancer cell lines RNA isolated...

Ngày tải lên: 20/06/2014, 07:20

... ovarian tumors and their potential role in ovarian cancer diagnosis and treatment Mucins Being that 90% of ovarian cancers are of epithelial origin, mucins may be attractive candidates for the ... above mentioned findings demonstrate the aberrant expression of mucins in ovarian cancer and show that mucin expression may alter the cellular characteristics of ovarian cancer cells and also imply ... present, MUC1 and MUC16 are the best and only characterized mucins and monoclonal antibodies against MUC1 and MUC16 are under preclinical and clinical investigations for ovarian cancer treatment...

Ngày tải lên: 20/06/2014, 07:20

... contains an abundance of epithelial cells B) LRG1 protein expression in 20 µg of total protein extract from ovarian cancer cell lines and immortalized NOSE cells b-actin was used as the loading ... ES-2, and OVCA429 ovarian cancer cell lines and all six of the NOSE cell lines Consistent with the Western blot, the cisplatinresistant cancer line A2780-CP demonstrated more intense staining compared ... OVCAR3 serous ovarian cancer cell lines, but not in the clear cell ovarian cancer cell line ES-2 [39] LRG1 peptides have also been identified in ascites fluid and cells from ovarian cancer patients...

Ngày tải lên: 20/06/2014, 07:20

... agent in prostate cancer [20] It will be interesting to determine whether this peptide may be useful in reversing drug resistance in ovarian cancer and we are currently investigating this enticing ... using Ingenuity Pathway Analysis A Network including ECM and other genes altered in cisplatin derived resistant cells B Network including proteasome genes and other genes altered in doxorubicin ... sub-lines The ovarian cancer cell line OV90 was obtained from The American Type Culture Collection (ATCC) and grown in MCDB 105 (Sigma-Aldrich):Media 199 (Invitrogen) containing 15% bovine serum and...

Ngày tải lên: 20/06/2014, 08:20

... DNA-PKcs is approximately 470 kD consisting of 4128 amino acids Its C-terminus contains a serine/threonine protein kinase domain (3719- 4128) [23] For screening Page of 11 the specific anti-DNA-PKcs ... the peptides were expressed in and purified from the infected E coli cells Upon inclusion body protein degeneration and refolding, we have finally successfully obtained the soluble DPK3 peptide ... protein level in HeLa, HeLa-pcDNA and HeLa-DPK3-scFv cells (B) In vitro detection of Gy-induced DNA-PKcs activity using the Signa-TECT® DNA-Dependent Protein Kinase Assay System Immunoblotting...

Ngày tải lên: 09/08/2014, 09:20

... NY) containing 10% (v/v) heat-inactivated fetal bovine serum (Gibco, Grand Island, NY), penicillin (100 U/mL) and streptomycin (100 mg/mL), and they were maintained in a 37°C humidified incubator ... Apoptosis induction by isoproterenol or propranolol in combination with irradiation in BGC-823 and SGC-7901 cells There was an increasing rate of apoptosis in gastric cancer cell lines in response ... activity in cancer cells, thus making the cells radioresistant [12] Activation of NF-B by various stimuli, including inflammation, stress and radiation, involves degradation of the inhibitory...

Ngày tải lên: 09/08/2014, 09:20

... ErPC3, in preclinical and clinical investigations is based on their ability to induce apoptosis in tumor cells and their ability to increase cytotoxic efficacy of chemotherapy and radiotherapy in ... lines and cell culture The prostate cancer cell lines LNCaP (p53 wild type, androgen-dependent, highly differentiated), PC3 (p53- /-, androgen-independent, poorly differentiated), and DU145 (p53 ... inhibitors of the PI3K/Akt pathway in prostate cancer cells Combined effects of ErPC3 and ionizing radiation in prostate cancer cell lines Up to now our data revealed that ErPC3 is a potent inhibitor...

Ngày tải lên: 09/08/2014, 09:20

... several protein tyrosine- and histidine kinases [810], and inhibits topoisomerase I and II [11] These effects result in alterations of several intracellular and extracellular pathways including cell ... radiosensitivity in the androgen independent PC cell line PC-3 has been demonstrated in vitro and in vivo [26,27] Our study analyzes the interactions of irradiation and genistein or estradiol incubation in androgen ... survival were ER and p53 independent Since genistein and estradiol effects in both cell lines were comparable, neither ER- nor p53- expression seemed to play a role in the linked signalling Nevertheless...

Ngày tải lên: 09/08/2014, 09:22