oxidative stress in neuronal cells and cyanide

... Ca2+ overloading and other factors including oxidative stress [88,89] In neurons the presence of PTP in situ has not gained wide acceptance among investigators and results published in the literature ... element binding protein, isocitrate dehydrogenase is an RNA-binding protein, while kgd2p is a mitochondrial DNA binding protein [81–84] Mitochondria from different brain regions contain different ... few minutes later leads to an irreversible and sustained increase in intracellular [Ca2+]c in apical dendrites [151] DCD is invariably demonstrated in every neuronal cell type studied, i.e spinal...

Ngày tải lên: 07/03/2014, 12:20

... senescence remains unclear Our findings, demonstrating the oxidative stress (ROS) induced telomere erosion and replicative senescence in chondrocytes, suggest the involvement of oxidative stress in both ... 4°C, and then paraffin blocks were prepared using standard histological procedures For nitrotyrosine staining, the sections were deparaffinized and hydrated, and then were immunostained using ... oxidative stress in OA cartilage, as discussed above, we postulated that oxidative stress induces telomere instability and dysfunction in chondrocytes, subsequently resulting in cartilage ageing and...

Ngày tải lên: 09/08/2014, 06:22

... containing PM10 and the other filter containing PM2.5 Preparation and extraction of filters Teflon filters were baked for h at 100oC and transferred into 50 ml conical tube with the particles facing ... study of PM2.5 - and PM10 - induced oxidative stress in rat lung epithelial cells I + form II) in PM2.5-treated DNA was significantly increased compared to both untreated-DNA and PM10treated DNA ... Jun-B, and Jun-D) proteins that bind with antioxidant response element (ARE) and regulate expression and induction of NADPH:quinone oxidoreductase and glutathione-S-transferase gene, encoding antioxidant...

Ngày tải lên: 07/08/2014, 17:22

... recycled to arginine by successive actions of argininosuccinate synthetase (AS; EC 6.3.4.5) and argininosuccinate lyase (AL; EC 4.3.2.1) via the citrulline-NO cycle (18) Arginine in brain is also utilized ... AL and arginase in the study AS and AL activities increased in all the three brain regions significantly in anoxia suggesting an increased utilization of citrulline for the production of arginine ... continuous supply of arginine at the site of synthesis Arginine is a semi essential amino acid and in CNS, its availability depends upon: (a) uptake from the circulating arginine and (b) recycling...

Ngày tải lên: 26/10/2012, 09:07

... activity, and the C-terminal domain, containing an iron-binding domain, which is involved in ADH activity The integrity of both domains and that of the iron-binding domain are required for ALDH ... 2010 FEBS 3383 Fermenting enzymes and oxidative stress in Entamoeba E Pineda et al Table Kinetic parameters of EhPFOR at 37 C Figures in parentheses indicate numbers of individual amebal extracts ... buffer and incubated for h on ice, and the remaining EhPFOR activity was determined A control experiment was prepared with a sample diluted in N2-purged buffer, and incubated for h on ice in the...

Ngày tải lên: 15/03/2014, 23:20

... COS-7 and SH-SY5Y cells generate different proNPFFA fragments indicating that a neuronal cell line possess a capacity of processing not found in non -neuronal cells The undecapeptide generated in ... Characterization and inhibition of a cholecystokinin-inactivating serine peptidase Nature 380, 403–409 Eipper, B.A., Milgram, S.L., Husten, E.J., Yun, H.Y & Mains, R.E (1993) Peptidylglycine alpha-amidating ... extend the N-terminal sequence of NPFF in mammals and recent reports demonstrating the importance of a protein convertase, the subtilisin/kexin isozyme SKI-1, in the processing of prohormones...

Ngày tải lên: 31/03/2014, 07:20

... apoptosis In fact, ROS-induced oxidative stress and cell death play important roles in the efficacy of many antineoplastic agents [13,14] To investigate whether oxidative stress is involved in the ... LOX inhibitors, including aesculetin (a nonselective LOX inhibitor), MK886 (an inhibitor of the 5-LOX-activating protein), zileuton (a 5-LOX inhibitor), baicalein (a 12/15-LOX inhibitor), and ... added, and cells were incubated for 40 at 37°C; cells were then returned to a prewarmed growth medium and incubated for 10 at 37°C Cells were harvested with trypsin and washed once with PBS, and...

Ngày tải lên: 18/06/2014, 16:20

... amyloidosis and brain inflammation when combined with indomethacin In summary, our findings support the hypothesis that oxidative stress and inflammation represent important but distinct therapeutic ... only a marginal effect on brain oxidative stress in the Tg2576 mice [16] This finding suggests that lipid peroxidation products contribute minimally to brain inflammation in this model, and raise ... animals were divided in two groups (n = 10 each), and randomized to receive placebo, or simultaneously indomethacin (10 mg/liter) in their drinking water, and vitamin E (α-tocopherol) in their diet...

Ngày tải lên: 19/06/2014, 22:20

... However, after 85 days, the NR activity in the seedlings was lower and decreased with increasing O3 At that time in the season and with increasing oxidative stress, NH3 may have been provided from ... of this cycle In the present study, GR activity increased two-fold in sugar maple seedlings in response to increasing O3 stress This enzyme ensures a high level of GSH regeneration and thus an ... of material from a single leaf was sampled from each ush of each seedling, cut into ì mm pieces and incubated in mL of 100 mM phosphate buer (pH 7.5) containing 40 mM KNO3 and 1.5% 1-propanol...

Ngày tải lên: 08/08/2014, 00:22

... factor, CD40 ligand, interleukin 1, or interleukin [10] Oxidative stress also promotes angiogenesis [13] The link between oxidative stress and angiogenesis is emphasised in SSc by the baseline correlation ... nifedipine can ameliorate endothelium injury in SSc and that VEGF signalling may by impaired in this disease without implication of the sVEGFR-1 Endothelium injury is critical in SSc and is suspected ... Friedlander M, Capelliere-Blandin C, NguyenKhoa T, Nguyen AT, Zingraff J, Jungers P, Descamps-Latscha B: Advanced oxidation protein products as a novel marker of oxidative stress in uremia Kidney Int...

Ngày tải lên: 09/08/2014, 01:23

... percentage relative to the respective control cells maintained in RGM **P < 0.01 and ***P < 0.001 relative to cells maintained in RGM Bars = mean ± standard deviation Page of 11 (page number not ... to the respective control cells maintained in RGM **P < 0.01 and ***P < 0.001 relative to the respective control cells maintained in RGM, §§§P < 0.001 between normal and OA chondrocytes exposed ... were prepared in RIPA buffer and the protein concentration was measured using the bicinchoninic acid/copper (II) sulphate protein assay kit (Sigma Chemical) The samples (25 μg protein) and molecular...

Ngày tải lên: 09/08/2014, 14:21

... effective in protecting PC12 cells from KA-induced injury in a dose-dependent manner GABA and PETL extract decreased KAinduced Ca2+ and ROS release and lipid peroxidation in PC12 cells and FVB ... implicated in apoptosis, the most prominent being caspases [51] However, we did find that KA affected the caspase-3 activation in PC12 cells Since S100 and COX2 may be involved in pathways leading to neuronal ... fixation Coronal paraffin sections were prepared with Hematoxylin and Eosin (H&E) staining for cells damage and TUNEL staining to assess apoptosis study After fixation for h, mice brain sections were...

Ngày tải lên: 10/08/2014, 10:20

... oxidative damage, we intend to further investigate the possible link between oxidative stress and mtDNA mutations mtDNA deletions/insertions and base changes mainly involve purine transitions, which ... not result in a significant difference in levels of oxidative stress biomarkers and the mtDNA copy number in dialysis patients Discussion An increased oxidative burden is observed in chronic ... have increased blood levels of oxidative stress biomarkers and an increased mtDNA copy number in peripheral leukocytes Since mitochondria are a major source of ROS and are highly susceptible to oxidative...

Ngày tải lên: 11/08/2014, 07:21

... apoptosis in some cells and proliferation in others Reports indicate either exacerbation or amelioration of pathological conditions in the brain during TNF- treatment, including experimentally induced ... with norepinephrine, dopamine and serotonin [32], to initiate their oxidation, which then continues with the production of more ROS, quinones, etc Damage to brain readily releases iron (and copper) ... higher than those in the active phase [27] indicating that in MS and probably in NMO, the regulatory cytokine, IL10, is impaired Regulatory mechanisms are essential in preventing autoimmune disorders...

Ngày tải lên: 11/08/2014, 08:22

... expression of iNOS (a), MPO (b), 4-HNE (c) and nitrotyrosine (d) in the induced sputum of non-smokers and smokers The expression of iNOS (a), MPO (b), 4-HNE (c) and nitrotyrosine (d) in the induced ... were performed and the mean value was used for analysis Sputum induction and processing A standard procedure of induction was conducted using 4.5% hypertonic saline given at 5-min intervals for ... lung function parameters than in non-smokers This is also in line with increased levels of lactoferrin in cigarette smokers, since lactoferrin is located in neutrophils In agreement with the negative...

Ngày tải lên: 12/08/2014, 16:20

... region and those coding for the N-terminal amino acid; the second contains the remaining 73 amino acids and a short tail of 3’-untranslated region and the third and the fourth contain the remaining ... protein kinase C substrate Rat Ng cDNA codes for a 78 amino acid protein which contains a conserved IQ motif that defines the overlapping region of CaM binding domain and PKC recoginition domain ... combination to study and solve many biological questions in the cells and will continue to progress and prosper in the future ERK MAPK pathway and its relations with learning and memory 6.1 Introduction...

Ngày tải lên: 05/10/2015, 22:32

... region and those coding for the N-terminal amino acid; the second contains the remaining 73 amino acids and a short tail of 3’-untranslated region and the third and the fourth contain the remaining ... protein kinase C substrate Rat Ng cDNA codes for a 78 amino acid protein which contains a conserved IQ motif that defines the overlapping region of CaM binding domain and PKC recoginition domain ... combination to study and solve many biological questions in the cells and will continue to progress and prosper in the future ERK MAPK pathway and its relations with learning and memory 6.1 Introduction...

Ngày tải lên: 06/10/2015, 20:35

... pilocarpine, on muscarinic and dopaminergic receptors and on phosphoinositide metabolism in rat hippocampus and striatum Neurochem Int 33, 299–306 21 Viana GSB, Marinho MMF, Sousa FCF, Bruin VMS, ... radicals [17] Neuronal hyperactivity and ⁄ or excitotoxicity may induce an increase in free radical concentrations during pilocarpine-induced SE [18] This work was performed to determine lipid peroxidation, ... cell and neuronal functions [8,18] The fact that an increase in catalase activity may not result in neurotoxic effects during SE indicates that basal ROS production is damaging to the neurons and...

Ngày tải lên: 19/02/2014, 16:20

... kinase, showed no effect on the DNA binding of c-Fos and c-Jun induced by treatment with ATZ + mercaptosuccinic acid, indicating that JNK is not involved in the binding of AP-1 to the bim promoter ... sustained oxidative stress This idea is supported by data showing that transient oxidative stress for or h did not induce apoptosis [38] In conclusion, ERK activation resulting from sustained oxidative ... In this experimental model, ERK was activated for h after the addition of ATZ + mercaptosuccinic acid, owing to inactivation of protein tyrosine phosphatase caused by sustained increases in intracellular...

Ngày tải lên: 06/03/2014, 00:21

... similar clinical symptoms to those observed in human VLCADD [5] In fact, in both mice and humans, clinical symptoms become mainly evident as a consequence of triggers such as fasting, resulting in the ... higher in VLCAD KO mice than in WT mice Fasting induced a significant increase in both genotypes; however, this was more evident in the VLCAD KO mice (Fig 3A) Interestingly, the MCT diet also induced ... 70.95 ± 4.4 UÆmg)1 in WT mice and 91.55 ± 8.5 UÆmg)1 in VLCAD KO mice in the nonfasted state Interestingly, the MCT diet combined with fasting significantly reduced GPX activity in WT mice, from...

Ngày tải lên: 15/03/2014, 23:20