oxidative stress in human infectious diseases present and current knowledge about its druggabi
Báo cáo y học: " Impaired glucose transporter-1 degradation and increased glucose transport and oxidative stress in response to high glucose in chondrocytes from osteoarthritic versus normal human cartilag" pps
Ngày tải lên :
09/08/2014, 14:21
... where its concentration is identical to and reflects that in the plasma, both under normal conditions and in noninflammatory and inflammatory types of arthritis, excluding those associated with infections ... by local inflammation and changes in the subchondral bone Increasing age, excessive loading or injury, genetic predisposition and obesity are important risk factors for the development and progression ... were prepared in RIPA buffer and the protein concentration was measured using the bicinchoninic acid/copper (II) sulphate protein assay kit (Sigma Chemical) The samples (25 μg protein) and molecular...
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Báo cáo y học: "NITRIC OXIDE (NO), CITRULLINE – NO CYCLE ENZYMES, GLUTAMINE SYNTHETASE AND OXIDATIVE STRESS IN ANOXIA (HYPOBARIC HYPOXIA) AND REPERFUSION IN RAT BRAIN"
Ngày tải lên :
26/10/2012, 09:07
... recycled to arginine by successive actions of argininosuccinate synthetase (AS; EC 6.3.4.5) and argininosuccinate lyase (AL; EC 4.3.2.1) via the citrulline-NO cycle (18) Arginine in brain is also utilized ... AL and arginase in the study AS and AL activities increased in all the three brain regions significantly in anoxia suggesting an increased utilization of citrulline for the production of arginine ... synthesis Arginine is a semi essential amino acid and in CNS, its availability depends upon: (a) uptake from the circulating arginine and (b) recycling of citrulline to argi- 152 nine by the actions...
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Báo cáo khoa học: Calcium, mitochondria and oxidative stress in neuronal pathology Novel aspects of an enduring theme pdf
Ngày tải lên :
07/03/2014, 12:20
... Ca2+ overloading and other factors including oxidative stress [88,89] In neurons the presence of PTP in situ has not gained wide acceptance among investigators and results published in the literature ... element binding protein, isocitrate dehydrogenase is an RNA-binding protein, while kgd2p is a mitochondrial DNA binding protein [81–84] Mitochondria from different brain regions contain different ... transients during reversible brain ischemia Exp Brain Res 104, 462–466 188 Silver IA & Erecinska M (1992) Ion homeostasis in rat brain in vivo: intra- and extracellular [Ca2+] and [H+] in the hippocampus...
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Báo cáo khoa học: Pyruvate:ferredoxin oxidoreductase and bifunctional aldehyde–alcohol dehydrogenase are essential for energy metabolism under oxidative stress in Entamoeba histolytica pdf
Ngày tải lên :
15/03/2014, 23:20
... activity, and the C-terminal domain, containing an iron-binding domain, which is involved in ADH activity The integrity of both domains and that of the iron-binding domain are required for ALDH ... 2010 FEBS 3383 Fermenting enzymes and oxidative stress in Entamoeba E Pineda et al Table Kinetic parameters of EhPFOR at 37 C Figures in parentheses indicate numbers of individual amebal extracts ... buffer and incubated for h on ice, and the remaining EhPFOR activity was determined A control experiment was prepared with a sample diluted in N2-purged buffer, and incubated for h on ice in the...
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The Management of Community-Acquired Pneumonia in Infants and Children Older Than 3 Months of Age: Clinical Practice Guidelines by the Pediatric Infectious Diseases Society and the Infectious Diseases Society of America pot
Ngày tải lên :
28/03/2014, 09:20
... as rhinovirus, RSV, human metapneumovirus, parainfluenza viruses, in uenza A and B viruses, adenovirus, coronavirus, and human bocavirus), and bacteria (including S pneumoniae, H in uenzae, and ... serious infections Inducible resistance to clindamycin may be present in staphylococci (ie, they may be D-test positive); for these strains, clindamycin should not be used in high-inoculum infections ... strains of in uenza A with adamantanes and neuraminidase inhibitors and for susceptible strains of in uenza B with neuraminidase inhibitors Because substantial genetic variation occurs in influenza...
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báo cáo hóa học: " Brain inflammation and oxidative stress in a transgenic mouse model of Alzheimer-like brain amyloidosis" pptx
Ngày tải lên :
19/06/2014, 22:20
... amyloidosis and brain inflammation when combined with indomethacin In summary, our findings support the hypothesis that oxidative stress and inflammation represent important but distinct therapeutic ... only a marginal effect on brain oxidative stress in the Tg2576 mice [16] This finding suggests that lipid peroxidation products contribute minimally to brain inflammation in this model, and raise ... animals were divided in two groups (n = 10 each), and randomized to receive placebo, or simultaneously indomethacin (10 mg/liter) in their drinking water, and vitamin E (α-tocopherol) in their diet...
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Báo cáo khoa học: "Comparative study of PM2.5 - and PM10 - induced oxidative stress in rat lung epithelial cells" pptx
Ngày tải lên :
07/08/2014, 17:22
... containing PM10 and the other filter containing PM2.5 Preparation and extraction of filters Teflon filters were baked for h at 100oC and transferred into 50 ml conical tube with the particles facing ... was quantified by measuring its absorbance at 260 nm and 280 nm Its integrity was confirmed by visualization of the ethidium bromide stained 28S and 18S ribosomal RNA bands in 1% agarose gel mRNA ... Jun-B, and Jun-D) proteins that bind with antioxidant response element (ARE) and regulate expression and induction of NADPH:quinone oxidoreductase and glutathione-S-transferase gene, encoding antioxidant...
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Báo cáo lâm nghiệp: "Response to an ozone gradient of growth and enzymes implicated in tolerance to oxidative stress in Acer saccharum (Marsh.) seedlings" pot
Ngày tải lên :
08/08/2014, 00:22
... However, after 85 days, the NR activity in the seedlings was lower and decreased with increasing O3 At that time in the season and with increasing oxidative stress, NH3 may have been provided from ... of this cycle In the present study, GR activity increased two-fold in sugar maple seedlings in response to increasing O3 stress This enzyme ensures a high level of GSH regeneration and thus an ... of material from a single leaf was sampled from each ush of each seedling, cut into ì mm pieces and incubated in mL of 100 mM phosphate buer (pH 7.5) containing 40 mM KNO3 and 1.5% 1-propanol...
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Báo cáo y học: "Nifedipine decreases sVCAM-1 concentrations and oxidative stress in systemic sclerosis but does not affect the concentrations of vascular endothelial growth factor or its soluble receptor" potx
Ngày tải lên :
09/08/2014, 01:23
... factor, CD40 ligand, interleukin 1, or interleukin [10] Oxidative stress also promotes angiogenesis [13] The link between oxidative stress and angiogenesis is emphasised in SSc by the baseline correlation ... nifedipine can ameliorate endothelium injury in SSc and that VEGF signalling may by impaired in this disease without implication of the sVEGFR-1 Endothelium injury is critical in SSc and is suspected ... Friedlander M, Capelliere-Blandin C, NguyenKhoa T, Nguyen AT, Zingraff J, Jungers P, Descamps-Latscha B: Advanced oxidation protein products as a novel marker of oxidative stress in uremia Kidney Int...
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Báo cáo y học: "Potential involvement of oxidative stress in cartilage senescence and development of osteoarthritis: oxidative stress induces chondrocyte telomere instability and downregulation of chondrocyte function" pptx
Ngày tải lên :
09/08/2014, 06:22
... senescence remains unclear Our findings, demonstrating the oxidative stress (ROS) induced telomere erosion and replicative senescence in chondrocytes, suggest the involvement of oxidative stress in both ... 4°C, and then paraffin blocks were prepared using standard histological procedures For nitrotyrosine staining, the sections were deparaffinized and hydrated, and then were immunostained using ... capable of producing ROS and NO [15,20,40] In the present study, stronger staining for nitrotyrosine, a marker of oxidative stress, was observed in degenerating regions as compared with intact regions...
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Báo cáo y học: "Pu-Erh tea and GABA attenuates oxidative stress in kainic acid-induced status epilepticu" docx
Ngày tải lên :
10/08/2014, 10:20
... Discussion The main result of the present study is the finding PETL and GABA protected animals from KA-induced brain injury MDA and apoptosis were significantly reduced in the GABA and PETL-treated ... release and increase in cell viability caused by the PETL extract and GABA were consistent with the in vivo data (B) 100 Figure DAPI and TUNEL staining of hippocampus form KAstressed mice KA induced ... fixation Coronal paraffin sections were prepared with Hematoxylin and Eosin (H&E) staining for cells damage and TUNEL staining to assess apoptosis study After fixation for h, mice brain sections were...
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Báo cáo khoa hoc:" Lack of association between mutations of gene-encoding mitochondrial D310 (displacement loop) mononucleotide repeat and oxidative stress in chronic dialysis patients in Taiwan" ppsx
Ngày tải lên :
11/08/2014, 07:21
... oxidative damage, we intend to further investigate the possible link between oxidative stress and mtDNA mutations mtDNA deletions/insertions and base changes mainly involve purine transitions, which ... not result in a significant difference in levels of oxidative stress biomarkers and the mtDNA copy number in dialysis patients Discussion An increased oxidative burden is observed in chronic ... have increased blood levels of oxidative stress biomarkers and an increased mtDNA copy number in peripheral leukocytes Since mitochondria are a major source of ROS and are highly susceptible to oxidative...
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Báo cáo y học: "TNF-α and IL-10 downregulation and marked oxidative stress in Neuromyelitis Optica" pptx
Ngày tải lên :
11/08/2014, 08:22
... Ethical Principles for Medical Research Involving Human Subjects of the World Medical Association ELISA Quantification of serum levels of cytokines, chemokine, matrix metalloproteinase and its inhibitor ... apoptosis in some cells and proliferation in others Reports indicate either exacerbation or amelioration of pathological conditions in the brain during TNF- treatment, including experimentally induced ... O2- is present, it can react with norepinephrine, dopamine and serotonin [32], to initiate their oxidation, which then continues with the production of more ROS, quinones, etc Damage to brain readily...
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Báo cáo y học: " Increased oxidative stress in asymptomatic current chronic smokers and GOLD stage 0 COPD" docx
Ngày tải lên :
12/08/2014, 16:20
... expression of iNOS (a), MPO (b), 4-HNE (c) and nitrotyrosine (d) in the induced sputum of non-smokers and smokers The expression of iNOS (a), MPO (b), 4-HNE (c) and nitrotyrosine (d) in the induced ... were performed and the mean value was used for analysis Sputum induction and processing A standard procedure of induction was conducted using 4.5% hypertonic saline given at 5-min intervals for ... condensate and induced sputum have been widely used in the indirect assessment of COPD and its progression [14,23] Of these techniques, exhaled air and induced sputum are relatively well standardized...
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Oxidative stress regulates DTNBPI dysbindin 1 expression and degradation via a pest sequence in it s c terminus
Ngày tải lên :
01/10/2015, 17:27
... Variation in the gene encoding dysbindin-1, dystrobrevin binding protein (DTNBP1), has been associated with schizophrenia Dysbindin-1 protein levels are reduced in several brain areas including the ... domain Excitotoxic injury and oxidative stress, triggered by intracerebroventricular kainate injections, resulted in increased number of dysbindin-1 expressing neurons in the dentate gyrus and ... protein found in humans but not expressed in rats Dysbindin-1C on the other hand is an isoform that lacks a NTR, and is a 270 amino acid protein It is detected in humans but its protein length in...
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Tài liệu Báo cáo khoa học: Oxidative stress in the hippocampus after pilocarpineinduced status epilepticus in Wistar rats doc
Ngày tải lên :
19/02/2014, 16:20
... pilocarpine, on muscarinic and dopaminergic receptors and on phosphoinositide metabolism in rat hippocampus and striatum Neurochem Int 33, 299–306 21 Viana GSB, Marinho MMF, Sousa FCF, Bruin VMS, ... Oxidative stress after status epilepticus in rats inefficiency of the electron-carrying components of the mitochondrial transport chain, monoamine degradation, xanthine oxidase reaction, and ... LMV & Pinho RSN (1997) Inhibitory action of a calcium channel blocker (nimodipine) on seizures and 1311 Oxidative stress after status epilepticus in rats 22 23 24 25 26 27 28 brain damage induced...
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Tài liệu Báo cáo khoa học: Role of cleavage and shedding in human thyrotropin receptor function and trafficking pdf
Ngày tải lên :
21/02/2014, 00:20
... this invariant sequence in the glycoprotein hormone receptors is required for proper folding, trafficking and ligand-mediated signaling but not for ligand binding [37,40] The complete loss in the ... (0–60 min) at 37 °C The concentration of the biotinylated Ig remaining on the cell surface was then quantified by measuring the binding of [125I]Streptavidin In the absence of bTSH, a very weak internalization ... Biochrom Bovine thyrotropin hormone (bTSH; IUÆmg)1), 3-iso-butyl-1-methylxanthine (IBMX), gelatin, BSA (bovine serum albumin; fraction V) and monensin were obtained from Sigma 2,2¢-Azine-di(ethylbenzthiazoline...
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Báo cáo khoa học: Fasting-induced oxidative stress in very long chain acyl-CoA dehydrogenase-deficient mice pdf
Ngày tải lên :
15/03/2014, 23:20
... similar clinical symptoms to those observed in human VLCADD [5] In fact, in both mice and humans, clinical symptoms become mainly evident as a consequence of triggers such as fasting, resulting in the ... higher in VLCAD KO mice than in WT mice Fasting induced a significant increase in both genotypes; however, this was more evident in the VLCAD KO mice (Fig 3A) Interestingly, the MCT diet also induced ... 70.95 ± 4.4 UÆmg)1 in WT mice and 91.55 ± 8.5 UÆmg)1 in VLCAD KO mice in the nonfasted state Interestingly, the MCT diet combined with fasting significantly reduced GPX activity in WT mice, from...
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Báo cáo Y học: Group IID heparin-binding secretory phospholipase A2 is expressed in human colon carcinoma cells and human mast cells and up-regulated in mouse inflammatory tissues doc
Ngày tải lên :
18/03/2014, 01:20
... cationic amino acid residues in their C-terminal regions [21,22] Since the multiple cationic residues in the corresponding C-terminal portions of mouse and human sPLA2-IIAs and rat and human sPLA2-Vs ... the fifth painting and subjected to RNA extraction Replicate ear sections were fixed by formalin, embedded in paraffin and stained with hematoxylin and eosin to verify the progress of in ammation ... Tokyo, Japan) containing 10% fetal bovine serum [8–12] cDNAs for human and mouse sPLA2s, human cyclooxygenase (COX)-2 and human microsomal prostaglandin E2 (PGE2) synthase (mPGES) and their HEK293...
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TRAFFICKING IN HUMAN BEINGS, ESPECIALLY WOMEN AND CHILDREN, IN AFRICA ppt
Ngày tải lên :
22/03/2014, 15:20
... TRAFFICKING IN HUMAN BEINGS, ESPECIALLY WOMEN AND CHILDREN, IN AFRICA 17 18 TRAFFICKING IN HUMAN BEINGS, ESPECIALLY WOMEN AND CHILDREN, IN AFRICA 2.8 SPECIAL CHALLENGES In analysing and mapping the ... Enhancing cooperation and coordination between relevant Ministries and Agencies in developing anti-trafficking policies and interventions - Developing policy and taking action against trafficking in ... traffickers, distinguishing between victims of trafficking and migrant smuggling and identifying traffickers, including those who are involved in controlling and exploiting trafficked persons Guideline 3:...
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