oxidative stress in heart failure

... arginase in the study AS and AL activities increased in all the three brain regions significantly in anoxia suggesting an increased utilization of citrulline for the production of arginine in ... observed by increase in NOx concentration in this study support the earlier findings of NO involvement in pathophysiology of hypobaric hypoxia in brain (42-43) In this study the increased activity ... citrulline generated as a by-product can be recycled to arginine by successive actions of argininosuccinate synthetase (AS; EC 6.3.4.5) and argininosuccinate lyase (AL; EC 4.3.2.1) via the citrulline-NO...

Ngày tải lên: 26/10/2012, 09:07

... lack of increase in LV dyssynchrony during dobutamine stress in patients with wide QRS duration (20) In contrast, Kurita et al reported on an increased mechanical dyssynchrony during pacing–induced ... pacing–induced tachycardia in patients with normal QRS duration (21) A remarkable increase in intra-LV dyssynchrony was also demonstrated during pharmacological stress in systolic heart failure patients ... rest or during exercise or the exercise-related increase in EDVI and the corresponding LDT values were found in both IDCM subgroups Results Systolic and diastolic time intervals The clinical characteristics...

Ngày tải lên: 05/11/2012, 11:32

... characterize oxidative stress Our findings show that pilocarpine induces SE, which can produce alterations in superoxide dismutase and catalase activities in different areas, thereby protecting the brain ... [2,19,20], immediately after pilocarpine administration, animals persistently show behavioral changes, including initial akinesia, ataxic lurching, peripheral cholinergic signs (miosis, piloerection, ... Oxidative stress after status epilepticus in rats inefficiency of the electron-carrying components of the mitochondrial transport chain, monoamine degradation, xanthine oxidase reaction,...

Ngày tải lên: 19/02/2014, 16:20

... functions, in addition to having supporting functions for oxidative defences [79], involving the thioredoxin system [40] Aconitase acts also as an iron-responsive element binding protein, isocitrate ... Ca2+ overloading and other factors including oxidative stress [88,89] In neurons the presence of PTP in situ has not gained wide acceptance among investigators and results published in the literature ... alterations in [Na+]i That may explain our previous results in isolated nerve terminals showing that in the presence of an oxidative stress a concomitant elevation in [Na+]i acts deleteriously on in situ...

Ngày tải lên: 07/03/2014, 12:20

... activity, and the C-terminal domain, containing an iron-binding domain, which is involved in ADH activity The integrity of both domains and that of the iron-binding domain are required for ALDH ... 2010 FEBS 3383 Fermenting enzymes and oxidative stress in Entamoeba E Pineda et al Table Kinetic parameters of EhPFOR at 37 C Figures in parentheses indicate numbers of individual amebal extracts ... et al Fermenting enzymes and oxidative stress in Entamoeba samples was also applied where indicated The number of independent preparations assayed is indicated in parentheses Modeling the EhPFOR...

Ngày tải lên: 15/03/2014, 23:20

... higher in VLCAD KO mice than in WT mice Fasting induced a significant increase in both genotypes; however, this was more evident in the VLCAD KO mice (Fig 3A) Interestingly, the MCT diet also induced ... observed in human VLCADD [5] In fact, in both mice and humans, clinical symptoms become mainly evident as a consequence of triggers such as fasting, resulting in the accumulation of long-chain acylcarnitines, ... protein utilizing the principle of protein-dye binding Anal Biochem 72, 248–254 FEBS Journal 277 (2010) 4699–4708 ª 2010 The Authors Journal compilation ª 2010 FEBS 4707 Oxidative stress in VLCAD...

Ngày tải lên: 15/03/2014, 23:20

... (2002) Invasive aspergillosis in 2002: an update Eur J Clin Microbiol Infect Dis 21, 161–172 Denning DW (1996) Therapeutic outcome in invasive aspergillosis Clin Infect Dis 23, 608–615 Denning DW ... Calmodulin forward Calmodulin reverse Aspergillus fumigatus actin forward Aspergillus fumigatus actin reverse 5¢ flanking forward 5¢ flanking reverse 3¢ flanking forward 3¢ flanking reverse zeocin–pyrG ... applied ROS Interestingly, upregulation of pes1 expression was not observed following H2O2-induced oxidative stress in cultures of A fumigatus 293.1 grown in either Sabouraud or 5% FBS in MEM (data...

Ngày tải lên: 30/03/2014, 10:20

... apoptosis In fact, ROS-induced oxidative stress and cell death play important roles in the efficacy of many antineoplastic agents [13,14] To investigate whether oxidative stress is involved in the ... several LOX inhibitors, including aesculetin (a nonselective LOX inhibitor), MK886 (an inhibitor of the 5-LOX-activating protein), zileuton (a 5-LOX inhibitor), baicalein (a 12/15-LOX inhibitor), ... decreased protein levels of MKP1, one of MAP kinase phosphatases that inactivate JNK and p38 MAP kinases [2] Interestingly, NSC-741909 induced an increase of MKP1 mRNA expression in both time-...

Ngày tải lên: 18/06/2014, 16:20

... that a full dose of indomethacin alone despite a significant reduction in brain inflammation had only a marginal effect on brain oxidative stress in the Tg2576 mice [16] This finding suggests that ... brain oxidative stress E supplementation on Effect of indomethacin plus vitamin E supplementation on markers of brain oxidative stress Total cerebral cortex homogenates from Tg2576 receiving ... pH 7.0) containing protease inhibitor mixture (pepstatin A, leupeptin, N-tosyl-L-phenylalanine chloromethyl ketone, http://www.jneuroinflammation.com/content/1/1/21 soybean trypsin inhibitor, each...

Ngày tải lên: 19/06/2014, 22:20

... has been investigated with the use of specific protein kinase inhibitors revealing that protein kinase C (PKC) [37-39], p21-activated kinase-1 (PAK1) [40], mitogen-activated protein kinase (MAPK) ... src-family tyrosine kinases or inhibition of phosphatidylinositol-3 kinase (PI3K) attenuates ROS production These findings suggest that these kinases are involved in upstream signaling cascades responsible ... together, these findings provide compelling in vitro evidence that vitamin E could act to inhibit the Aβ-stimulated microglia inflammatory response Specifically, vitamin E could inhibit ROS and...

Ngày tải lên: 19/06/2014, 22:20

... origin, was obtained from ATCC (Manassas, USA) and cultured Ham’s F-12 media (Gibco) containing mM L-glutamine supplemented with 0.01 mg/ml bovine pituitary extract (Gibco, USA), 0.005 mg/ml insulin ... PM10 to oxidative stress repair enzyme, we used RT-PCR analysis Figure shows the expression level of catalase mRNA induced by Comparative study of PM2.5 - and PM10 - induced oxidative stress in rat ... PM2.5 induced more MDA formation than PM10 suggesting that PM2.5 induced more oxidative stress than PM10 In the single strand breakage assay using pBR322 DNA, the ratio of changed form II/total...

Ngày tải lên: 07/08/2014, 17:22

... seedling, cut into ì mm pieces and incubated in mL of 100 mM phosphate buer (pH 7.5) containing 40 mM KNO3 and 1.5% 1-propanol Each sample was vortexed for to help tissue inltration by the incubation ... CO2 by PEPC increased in response to O3 in both groups of seedlings (Figs 5E5H) during the growing season The level of activity decreased in the neoformed ush of the episodic seedlings when the ... [14] GR is one of the enzymes of this cycle In the present study, GR activity increased two-fold in sugar maple seedlings in response to increasing O3 stress This enzyme ensures a high level of...

Ngày tải lên: 08/08/2014, 00:22

... including hypoxic conditions and stimulation by transforming growth factor, CD40 ligand, interleukin 1, or interleukin [10] Oxidative stress also promotes angiogenesis [13] The link between oxidative ... 14 (7.4 ± 2) Ongoing angiotensin-converting enzyme inhibitors (no patients) Ongoing low-dose aspirin therapy (no patients) 25 Values are number (%) of patients unless otherwise indicated DLCO, ... carbon monoxide diffusion in the lung; Hb, hemoglobin in ml of M guanidine in 20 mM potassium phosphate, adjusted to pH 2.3 with trifluoracetic acid; the resulting solution was incubated at 37°C for...

Ngày tải lên: 09/08/2014, 01:23

... Conclusion This study provides insight into the involvement of oxidative stress in the pathogenesis of OA from the viewpoint of oxidative stress induced genomic instability, especially telomere ... degeneration in cartilage The findings of the present study suggest that cumulative oxidative stress leads to a decrease in antioxidative capacity in articular cartilage, resulting in chondrocyte ... replication Oxidative stress increases the telomere shortening rate by up to one order of magnitude [46] From these findings, we postulated that oxidative stress directly induces chondrocyte telomere instability...

Ngày tải lên: 09/08/2014, 06:22

... lysosome in mediating high-glucose-induced downregulation of glucose transporter-1 protein Glucose transprotein porter-1 (GLUT-1) protein content normalized to the respective actin band in normal ... Nonproliferating monolayer cultures were established from each cartilage sample, allowed to recover in medium containing 5% fetal bovine serum for 24 hours, serum-starved overnight and maintained thereafter ... protein concentration Western blot analysis Whole cell lysates were prepared in RIPA buffer and the protein concentration was measured using the bicinchoninic acid/copper (II) sulphate protein...

Ngày tải lên: 09/08/2014, 14:21

... to investigate the protective mechanisms of GABA and Pu-erh tea leaf extract on KA-induced injury in neuronal cells in vivo and in vitro Methods Materials GABA and kainic acid (KA) were obtained ... factors, including members of the S100 protein family [45,46], act to sustain the inflammatory response or to determine direct effects on neurons and/or microglia, thus switching the inflammatory ... L, Hartley O, Dubois-Dauphin M, Krause KH: Chemokine receptors in the central nervous system: role in brain inflammation and neurodegenerative diseases Brain Res Brain Res Rev 2005, 48:16-42...

Ngày tải lên: 10/08/2014, 10:20

... removed[15,22,23] Inducible NOS (iNOS) activity and total NOS activity in spinal cord were measured with a NOS kit (Nanjing Jiancheng Institute of Biology and Engineering, Nanjing, China), which assessed ... by measuring the conversion of L-[14C]-arginine to L-[14C]citrulline [24] The total NOS activity was determined by incubating samples (50 μL) for 15 at 37°C in a reaction mixture containing buffer ... nicotinamide adenine dinucleotide phosphate(β-NADPH), mM CaCl2, 50 μM tetrahydrobiopterin (BH4) and μCi/ ml L-[14C]-arginine Inducible NOS (iNOS) activity was measured by omitting calcium and adding...

Ngày tải lên: 10/08/2014, 10:20

... result in a significant difference in levels of oxidative stress biomarkers and the mtDNA copy number in dialysis patients Discussion An increased oxidative burden is observed in chronic renal failure ... of our findings, we have proposed a possible gateway to further investigate the association between mtDNA mutations and oxidative stress in chronic renal failure References Competing interests ... susceptible region for oxidative stress, we hypothesized that the D-loop mutation exists in dialysis patients with an increased oxidative stress status In this study, we investigated that hypothesis...

Ngày tải lên: 11/08/2014, 07:21

... apoptosis in some cells and proliferation in others Reports indicate either exacerbation or amelioration of pathological conditions in the brain during TNF- treatment, including experimentally induced ... higher than those in the active phase [27] indicating that in MS and probably in NMO, the regulatory cytokine, IL10, is impaired Regulatory mechanisms are essential in preventing autoimmune disorders ... can react with norepinephrine, dopamine and serotonin [32], to initiate their oxidation, which then continues with the production of more ROS, quinones, etc Damage to brain readily releases iron...

Ngày tải lên: 11/08/2014, 08:22

... who have heart failure worldwide Heart failure is primarily a disease of the elderly, and the prevalence of chronic heart failure increases with advancing age Currently, chronic heart failure ... chronic shortage of donor hearts Currently, the mainstay of heart failure treatment is pharmacologic and includes angiotensin converting enzyme inhibitors, angiotensin receptor blockers, b-adrenergic ... diastolic heart failure ranges from 5% to 15%, and admission rate for recurrent heart failure is 50% within the first months [1–4] The remaining 50% to 70% of patients present with systolic heart failure...

Ngày tải lên: 11/08/2014, 15:20