oxidative stress in applied basic research and clinical practice

... receptor NLRP3 (NOD-, LRRand pyrin domain-containing 3) and the PYHIN (pyrin and HIN200 domain-containing) family protein AIM2 (absent in melanoma 2) are two PRRs that survilliance intracelluar DAMPs ... future  research.   The  book  is  targeted  at  researchers  and clinicians  who  are  interested  in learning  about  new  advances  in the  understanding and treatment of AMD, and insights into future research of AMD.  ... levels increase Also antioxidant vitamins, such as vitamin C and E, show a decline with increasing age (Rikans and Moore, 1988; Vandewoude and Vandewoude, 1987) In support of oxidation stress...

Ngày tải lên: 27/06/2014, 15:20

... ‘implementing the findings from research in clinical practice. ’ To make a significant contribution to the national and international evidence base concerning research use and impact, and mechanisms ... implementation research agenda and recommended sustained and strategic investment in research and infrastructure aimed at increasing our capability and capacity to maximise the impact of health research ... include sensing and interpreting new evidence, integrating it with existing evidence; reinforcement (or not) by professional networks, which in turn is mediated by local context [23], including...

Ngày tải lên: 10/08/2014, 11:20

... recycled to arginine by successive actions of argininosuccinate synthetase (AS; EC 6.3.4.5) and argininosuccinate lyase (AL; EC 4.3.2.1) via the citrulline-NO cycle (18) Arginine in brain is also utilized ... AL and arginase in the study AS and AL activities increased in all the three brain regions significantly in anoxia suggesting an increased utilization of citrulline for the production of arginine ... uptake from the circulating arginine and (b) recycling of citrulline to argi- 152 nine by the actions of AS and AL L-arginine is transported into synaptosomes (46), neurons (47) and astroglia (48)...

Ngày tải lên: 26/10/2012, 09:07

... have delineated the potential beneficial activities of HGF in current clinical use, including studies exploring dosing and scheduling parameters that inform the clinical use of these agents in the ... facilitated dose finding and clinical development Science now moves more quickly, and there is an expectation that basic discoveries can be applied clinically in 2–3 years Perhaps an understanding of how ... and clinical use Blood 1997; 89:3897–3908 113 Turner KJ, Clark SC Interleukin-11: Biological and clinical perspectives In: Mertelsmann R, Herrmann F, eds., Hematopoietic Growth Factors in Clinical...

Ngày tải lên: 16/02/2014, 07:20

... albumin, S total protein, U protein (dipstick) S transferrin, prealbumin, S retinol-binding protein, creatinine height index U hydroxyproline index; S amino acid profiles, indices; nitrogen balance; ... C, D ↓Vitamin D ↓Vitamin C ↓Protein, calories ↓Thiamin ↓Thiamin, vitamin E ↓Vitamin E ↓Vitamins B12, E ↓Iodine ↓Chromium ↓Zinc ↓Vitamin C, zinc Adapted from Hubbard VA, Hubbard LR Clinical assessment ... understand its significance for development, and apply it to clinical practice Functional specialties in medicine increasingly are interacting in a matrix fashion with organ-based specialties Clinical...

Ngày tải lên: 16/02/2014, 07:20

... Summary In this article, we outline a model for thinking about how organizations can influence community clinicians’ decision-making about becoming or remaining involved in clinical research ... Practice- based clinical research and ethical decision making–Part I: deciding whether to incorporate practice- based research into your clinical practice Semin Neurol 2006, 26(1): 131-9 16 Pinto HA, ... should increase the likelihood that clinicians will choose – in a sustained way – to incorporate into their practices research that could inform clinical questions, methods, analyses, and clinical...

Ngày tải lên: 10/08/2014, 10:23

... Health Research Funding [6] They have three key interlinked functions: conducting high quality applied health research; implementing the findings from research in clinical practice; and increasing ... Type Examples Knowledge brokers Clinical managers Clinicians involved in management Clinicians involved in research Clinicians involved in quality improvement Internal facilitators (e.g., knowledge ... Service (NHS) Trusts aimed at conducting applied health research and translating its findings into day-to-day clinical practice It will briefly discuss some of the seminal theoretical CoP literature...

Ngày tải lên: 10/08/2014, 11:20

... translating ideas from basic and clinical research into the development of new products and approaches to treatment of disease and illness, and implementing those new products and approaches into clinical ... three related objectives, namely: conducting high-quality applied health research; implementing the findings from research in clinical practice; and increasing the capacity of National Health Service ... dissemination, continuing professional development, and clinical audit could be used to promote uptake of the research in practice Linear models of research use typically view research and practice...

Ngày tải lên: 10/08/2014, 11:20

... part of research translation; and increase local capacity in applied research It has a combination of four inter-related applied research themes and an implementation theme (Table 1) and is focused ... conducting applied research and translating findings into improved outcomes To date, nine CLAHRCs have been established, one of which is that of Leicestershire, Northamptonshire, and Rutland (LNR), ... Leadership in Applied Health Research and Care (CLAHRCs) are new organisations funded by the National Institute for Health Research (NIHR) in England to conduct and implement applied health research, ...

Ngày tải lên: 11/08/2014, 05:21

... part of research translation; and increase local capacity in applied research It has a combination of four inter-related applied research themes and an implementation theme (Table 1) and is focused ... conducting applied research and translating findings into improved outcomes To date, nine CLAHRCs have been established, one of which is that of Leicestershire, Northamptonshire, and Rutland (LNR), ... Leadership in Applied Health Research and Care (CLAHRCs) are new organisations funded by the National Institute for Health Research (NIHR) in England to conduct and implement applied health research, ...

Ngày tải lên: 11/08/2014, 16:20

... participating in research Professionals directing clinical research networks involving research in community practices that could serve as prototypes for research in community settings Representatives ... of clinical research into clinical practice, ranging from the need to evaluate whether treatments shown to be efficacious in clinical trials continue to be effective in real-world practice, and ... companies, clinical research organizations, and other organizations that have recruited and trained community healthcare providers for clinical trials and clinical research networks and/ or have key information...

Ngày tải lên: 10/08/2014, 10:23

... Ca2+ overloading and other factors including oxidative stress [88,89] In neurons the presence of PTP in situ has not gained wide acceptance among investigators and results published in the literature ... element binding protein, isocitrate dehydrogenase is an RNA-binding protein, while kgd2p is a mitochondrial DNA binding protein [81–84] Mitochondria from different brain regions contain different ... transients during reversible brain ischemia Exp Brain Res 104, 462–466 188 Silver IA & Erecinska M (1992) Ion homeostasis in rat brain in vivo: intra- and extracellular [Ca2+] and [H+] in the hippocampus...

Ngày tải lên: 07/03/2014, 12:20

... activity, and the C-terminal domain, containing an iron-binding domain, which is involved in ADH activity The integrity of both domains and that of the iron-binding domain are required for ALDH ... 2010 FEBS 3383 Fermenting enzymes and oxidative stress in Entamoeba E Pineda et al Table Kinetic parameters of EhPFOR at 37 C Figures in parentheses indicate numbers of individual amebal extracts ... buffer and incubated for h on ice, and the remaining EhPFOR activity was determined A control experiment was prepared with a sample diluted in N2-purged buffer, and incubated for h on ice in the...

Ngày tải lên: 15/03/2014, 23:20

... Banking Trim Size: 9in x 6in Need for Radiation Sterilization of Tissue Grafts ch02 15 contamination originating from the environment and handling Additional handling during processing can cause additional ... scrubbing and proper gowning before entering the processing room They should always cover their hair (including beard) and wear a mask, and must not talk or cough during procurement and processing ... RADIATION IN TISSUE BANKING Basic Science and Clinical Applications of Irradiated Tissue Allografts March 19, 2007 14:26 b478 Radiation in Tissue Banking Trim Size: 9in x 6in This page intentionally...

Ngày tải lên: 22/03/2014, 20:21

... amyloidosis and brain inflammation when combined with indomethacin In summary, our findings support the hypothesis that oxidative stress and inflammation represent important but distinct therapeutic ... only a marginal effect on brain oxidative stress in the Tg2576 mice [16] This finding suggests that lipid peroxidation products contribute minimally to brain inflammation in this model, and raise ... animals were divided in two groups (n = 10 each), and randomized to receive placebo, or simultaneously indomethacin (10 mg/liter) in their drinking water, and vitamin E (α-tocopherol) in their diet...

Ngày tải lên: 19/06/2014, 22:20

... containing PM10 and the other filter containing PM2.5 Preparation and extraction of filters Teflon filters were baked for h at 100oC and transferred into 50 ml conical tube with the particles facing ... Jun-B, and Jun-D) proteins that bind with antioxidant response element (ARE) and regulate expression and induction of NADPH:quinone oxidoreductase and glutathione-S-transferase gene, encoding antioxidant ... PM2.5 induced more MDA formation than PM10 suggesting that PM2.5 induced more oxidative stress than PM10 In the single strand breakage assay using pBR322 DNA, the ratio of changed form II/total...

Ngày tải lên: 07/08/2014, 17:22

... However, after 85 days, the NR activity in the seedlings was lower and decreased with increasing O3 At that time in the season and with increasing oxidative stress, NH3 may have been provided from ... of this cycle In the present study, GR activity increased two-fold in sugar maple seedlings in response to increasing O3 stress This enzyme ensures a high level of GSH regeneration and thus an ... of material from a single leaf was sampled from each ush of each seedling, cut into ì mm pieces and incubated in mL of 100 mM phosphate buer (pH 7.5) containing 40 mM KNO3 and 1.5% 1-propanol...

Ngày tải lên: 08/08/2014, 00:22

... factor, CD40 ligand, interleukin 1, or interleukin [10] Oxidative stress also promotes angiogenesis [13] The link between oxidative stress and angiogenesis is emphasised in SSc by the baseline correlation ... nifedipine can ameliorate endothelium injury in SSc and that VEGF signalling may by impaired in this disease without implication of the sVEGFR-1 Endothelium injury is critical in SSc and is suspected ... Friedlander M, Capelliere-Blandin C, NguyenKhoa T, Nguyen AT, Zingraff J, Jungers P, Descamps-Latscha B: Advanced oxidation protein products as a novel marker of oxidative stress in uremia Kidney Int...

Ngày tải lên: 09/08/2014, 01:23

... senescence remains unclear Our findings, demonstrating the oxidative stress (ROS) induced telomere erosion and replicative senescence in chondrocytes, suggest the involvement of oxidative stress in both ... 4°C, and then paraffin blocks were prepared using standard histological procedures For nitrotyrosine staining, the sections were deparaffinized and hydrated, and then were immunostained using ... oxidative stress in OA cartilage, as discussed above, we postulated that oxidative stress induces telomere instability and dysfunction in chondrocytes, subsequently resulting in cartilage ageing and...

Ngày tải lên: 09/08/2014, 06:22

... by local inflammation and changes in the subchondral bone Increasing age, excessive loading or injury, genetic predisposition and obesity are important risk factors for the development and progression ... concentration is identical to and reflects that in the plasma, both under normal conditions and in noninflammatory and inflammatory types of arthritis, excluding those associated with infections [12] To ... were prepared in RIPA buffer and the protein concentration was measured using the bicinchoninic acid/copper (II) sulphate protein assay kit (Sigma Chemical) The samples (25 μg protein) and molecular...

Ngày tải lên: 09/08/2014, 14:21