oxidative stress in alzheimers disease

... global inhibition of src-family tyrosine kinases or inhibition of phosphati- dylinositol-3 kinase (PI3K) attenuates ROS production. These findings suggest that these kinases are involved in upstream ... has been investi- gated with the use of specific protein kinase inhibitors revealing that protein kinase C (PKC) [37-39], p21-acti- vated kinase-1 (PAK1) [40], mitogen-activated protein kinase (MAPK) ... Peroxynitrite-induced alterations in synapto- somal membrane proteins: insight into oxidative stress in Alzheimer's disease. J Neurochem 1999, 72:310-317. 87. Bonfoco E, Krainc D, Ankarcrona...

Ngày tải lên: 19/06/2014, 22:20

... synthase. The increased activities of argininosuccinate synthetase and argininosuccinate lyase suggest the increased and effective recycling of citrulline to arginine in anoxia, making nitric ... in brain in anoxia as observed by in- crease in NOx concentration in this study support the earlier findings of NO involvement in pathophysiol- ogy of hypobaric hypoxia in brain (42-43). In this ... arginine at the site of synthesis. Arginine is a semi essential amino acid and in CNS, its availability depends upon: (a) uptake from the circu- lating arginine and (b) recycling of citrulline...

Ngày tải lên: 26/10/2012, 09:07

... oxidative stress. Our findings show that pilocarpine induces SE, which can produce alterations in superoxide dismutase and catalase activities in different areas, thereby pro- tecting the brain ... [2,19,20], immediately after pilocarpine administration, animals persistently show behavioral changes, including initial akinesia, ataxic lurching, peripheral cholinergic signs (miosis, piloerection, ... Interstrain differences in cognitive functions in rats in relation to status epilepticus. Behav Brain Res 112, 77–83. 3 Treiman DM (1955) Electroclinical features of status epilepticus. J Clin...

Ngày tải lên: 19/02/2014, 16:20

... neuron. The turning point upon which the cell looses the ability to buffer the incoming calcium resulting in an abrupt, sustained and irreversible increase in [Ca 2+ ] c , probably coincides with ... functions, in addition to having supporting functions for oxidative defences [79], involving the thioredoxin system [40]. Aconitase acts also as an iron-responsive element binding protein, iso- citrate ... by proteins resi- ding in both the inner and outer mitochondrial membrane, that is activated by mitochondrial Ca 2+ overloading and other factors including oxidative stress [88,89]. In neurons...

Ngày tải lên: 07/03/2014, 12:20

... indicating the presence of two catalytically independent domains, the N-terminal domain, display- ing ALDH activity, and the C-terminal domain, con- taining an iron-binding domain, which is involved ... against oxidative stress damage, have not been reported. In vivo effects on glucose-fermenting enzymes and fluxes under oxidative stress Recently, we reported that amebas incubated for 30 min in ... bind close to the Fe–S clusters, blocking the access of ROS. In this regard, a preliminary docking analysis with coenzymes in the 3D model of EhPFOR suggested that the CoA-binding site was, indeed,...

Ngày tải lên: 15/03/2014, 23:20

... mice. Fasting induced a significant increase in both genotypes; however, this was more evident in the VLCAD KO mice (Fig. 3A). Interestingly, the MCT diet also induced AOX gene expression in WT mice. After ... observed in human VLCADD [5]. In fact, in both mice and humans, clinical symptoms become mainly evident as a consequence of triggers such as fasting, resulting in the accumulation of long-chain acylcarnitines, ... quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72, 248–254. S. Tucci et al. Oxidative stress in VLCAD KO mice FEBS Journal 277 (2010)...

Ngày tải lên: 15/03/2014, 23:20

... to applied ROS. Interestingly, upregulation of pes1 expression was not observed following H 2 O 2 -induced oxidative stress in cultures of A. fumigatus 293.1 grown in either Sabouraud or 5% FBS in MEM ... Infect Dis 21, 161–172. 4 Denning DW (1996) Therapeutic outcome in invasive aspergillosis. Clin Infect Dis 23, 608–615. 5 Denning DW (1998) Invasive aspergillosis. Clin Infect Dis 26, 781–805. 6 ... this strain in subsequent gene-disruption experi- ments. In order to find whether pes1 was expressed during fungal infection in G. mellonella, A. fumigatus ATCC 26933 conidia were injected into larvae...

Ngày tải lên: 30/03/2014, 10:20

... several LOX inhibitors, including aesculetin (a nonselective LOX inhibitor), MK886 (an inhibitor of the 5-LOX-activating protein), zileuton (a 5-LOX inhibitor), baicalein (a 12/15-LOX inhibitor), ... apoptosis. In fact, ROS-induced oxidative stress and cell death play important roles in the efficacy of many antineoplastic agents [13,14]. To investigate whether oxidative stress is involved in the ... National Cancer Institute. Using a reverse-phase pro- tein microarray assay, we determined molecular changes in 77 protein biomarkers in an oncrasin-sensitive lung cancer cell line after treatment...

Ngày tải lên: 18/06/2014, 16:20

... brain Aβ amyloidosis model. Previously, we have shown that a full dose of indomethacin alone despite a significant reduction in brain inflammation had only a marginal effect on brain oxidative stress ... inflammation when combined with indomethacin. In summary, our findings support the hypothesis that oxi- dative stress and inflammation represent important but distinct therapeutic targets in AD-like amyloidosis. ... domenico@spirit.gcrc.upenn.edu * Corresponding author Abstract Background: An increasing body of evidence implicates both brain inflammation and oxidative stress in the pathogenesis of Alzheimer's disease (AD). The...

Ngày tải lên: 19/06/2014, 22:20

... (Gibco) containing 2 mM L-glutamine supplemented with 0.01 mg/ml bovine pituitary extract (Gibco, USA), 0.005 mg/ml insulin (Sigma, USA), 2.5 ng/ml IGF (Sigma), 0.025 mg/ml trasferrin (Sigma, USA), ... study, PM2.5 induced more MDA formation than PM10 suggesting that PM2.5 induced more oxidative stress than PM10. In the single strand breakage assay using pBR322 DNA, the ratio of changed form II/total ... protein in the PM2.5 - and PM10 - t reated rat lung epithelial cell was determined by Weste rn B lotting. The lower shows the ratio of Nrf2/actin usi ng c alculatging by densitometor. In the...

Ngày tải lên: 07/08/2014, 17:22

... CO 2 by PEPC increased in re- sponse to O 3 in both groups of seedlings (Figs. 5E–5H) dur- ing the growing season. The level of activity decreased in the neoformed flush of the episodic seedlings when ... seedling, cut into 2 ì 2 mm pieces and incubated in 5 mL of 100 mM phosphate buffer (pH 7.5) containing 40 mM KNO 3 and 1.5% 1-propanol. Each sample was vortexed for 2 min to help tissue in ltration ... by immersing the tubes for 5 min in boiling water. The colorimetric determination of NO 2 − was done by mixing 0.25 mL of incubation medium with 0.25 mL 0.02% N-(1-Naphtyl) ethylenediamine and...

Ngày tải lên: 08/08/2014, 00:22

... production, including hypoxic conditions and stimulation by transforming growth factor, CD40 ligand, interleukin 1, or interleukin 6 [10]. Oxi- dative stress also promotes angiogenesis [13]. The link between ... suggest that nifedipine can ameliorate endothelium injury in SSc and that VEGF signalling may by impaired in this disease without implication of the sVEGFR-1. Endothelium injury is critical in SSc and ... VEGF pathway is intrinsically defective in SSc. Keywords: nifedipine, oxidative stress, sVCAM-1, systemic sclerosis, VEGF Introduction Systemic sclerosis (SSc) is a connective tissue disease characterised...

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