oxidative stress defense mechanisms in plants

... with oxidative stress, we also measured several oxi- dative stress related gene-products. To determine a possible damaging effect of the oxidative stress, we inves- tigated proteins involved in ... copper incorpo- rated during biosynthesis. Metallothionein 1A (MT1A) is a small intracellular protein capable of chelating several metal ions, including copper. It contains many cysteine residues, ... for maintaining intracellular copper concentrations [3]. If this balance is disturbed, excess cop- per can induce oxidative stress that could lead to chronic inflammation [4,5]. Copper induced...

Ngày tải lên: 13/08/2014, 13:20

... and behavioral programming and regulation, and may thus provide information on the ability of the individual to adjust to stressful situations In fact, coping and defense mechanisms are not only ... Defenses here are defined as reactions of which the individual is unaware, reflecting both innate traits and learned coping mechanisms which are not necessarily pathological and may exclude information ... correlations coefficient) in order to identify variables to be included in the multivariate ANCOVA models P-values from multivariate ANCOVA models, indicating Defense Mechanisms and Coping Styles associated...

Ngày tải lên: 10/01/2020, 13:42

... augmentation of ATO-induced apoptosis remains unclear In particular, the molecular events in mitochondria involved in increased apoptotic susceptibility are unknown In this study we investigated the ... lymphoma protein; BCLxL: BCL2-like X protein; JNK: c-JUN N-terminal kinase; ERK1/2: Extracellular signal-regulated kinase 1/2; ASK1: Apoptosis signal-regulating kinase Page 10 of 11 Competing interests ... mitochondrial injury In the preceding section, BSO augmented ATO-induced cell death via intracellular ROS generation To clarify involvement of ROS-mediated mitochondrial injury in BSO-mediated...

Ngày tải lên: 05/11/2020, 02:00

... of left ventricular remodeling, oxidative stress and inflammation in obese mice Hui-Ting Wang1, Chu-Feng Liu1†, Tzu-Hsien Tsai2, Yung-Lung Chen2, Hsueh-Wen Chang3, Ching-Yen Tsai4, Steve Leu5, ... the effect of obesity reduction on preserving left ventricular ejection fraction (LVEF) and attenuating inflammation, oxidative stress and LV remodeling in obese mice Methods and results: Eight-week-old ... myocardium collected Heart weight, body weight, abdominal-fat weight, total cholesterol level and fasting blood glucose were higher in obesity than in control and OR (all p...

Ngày tải lên: 02/11/2022, 09:23

... C groups; γ-radiation increased oxidative stress (OS), LPO, apoptosis and induced testes injuries These results are in agreement with the histopathological examina‑ tion In contrast, treatment ... carried out to investigate the role of H in minimizing the testis damage induced by γ-radiation in a total dose of 8 Gy The study focused on the determination of apoptotic markers (DNAF in testes ... treatment of venous insufficiency and hemorrhoids [9] A deficiency of H in the diet has been linked with abnormal capillary leakiness as well as pain in the extremities causing aches, weakness...

Ngày tải lên: 04/12/2022, 16:05

... to oxidative stress in transgenic plants during seed germination 1372 Plants have evolved antioxidant defense systems to dispose of excess ROS and to maintain cellular ROS homeostasis [17] To investigate ... protein kinase and woundinduced protein kinase, two MAPKs in group A, were activated by inoculation with the tobacco mosaic virus (TMV) [10] Their orthologs in other plant species, including MPK3 ... accumulation in wild-type and transgenic plants in response to abiotic and abiotic stresses (A) Virus infection-induced H2O2 accumulation detected by DAB staining (B) Abiotic stress- induced H2O2...

Ngày tải lên: 14/03/2014, 23:20

... long chain fatty acids (Elovl3), type iodothyronine deiondinase (Dio2), homeobox C9 (Hoxc9), insulin-like growth factor binding protein (Igfbp3), dermatopontin (Dpt), and b-actin are showed in Table ... deviation inflammatory response and IR development, were examined in mice As shown in Figure 3, PM 2.5 exposure induced a marked increase in macrophage (F4/80+ cells) infiltration in eWAT Next, ... specific genes in different adipose depots There are several major findings in this study First, exposure to PM2.5 resulted in oxidative stress in BAT Second, exposure to PM 2.5 induced changes...

Ngày tải lên: 06/03/2014, 19:20

... functions, in addition to having supporting functions for oxidative defences [79], involving the thioredoxin system [40]. Aconitase acts also as an iron-responsive element binding protein, iso- citrate ... Ca 2+ overloading and other factors including oxidative stress [88,89]. In neurons the presence of PTP in situ has not gained wide acceptance among investigators and results published in the literature ... neuron. The turning point upon which the cell looses the ability to buffer the incoming calcium resulting in an abrupt, sustained and irreversible increase in [Ca 2+ ] c , probably coincides with...

Ngày tải lên: 07/03/2014, 12:20

... enzymatic activity involved in the oxidative stress in S. solfataricus. To detect differences in the response to various agents we induced oxidative stress with paraquat, an O 2 • ) generating compound, ... stress conditions, are important in deter- mining the level of Bcp2 protein. Similar results are observed for genes and related proteins involved in oxidative stress in other microrganisms [24]. ... S p peroxidatic cysteine; X unidentified electron donor; S R resolving cysteine; RSH protein or domain with CXXC motif (e.g. thioredoxin). In typical 2-Cys, S p at the N terminus and S R at the C terminus belong...

Ngày tải lên: 07/03/2014, 12:20

... activity, and the C-terminal domain, containing an iron-binding domain, which is involved in ADH activity The integrity of both domains and that of the iron-binding domain are required for ALDH ... E Pineda et al Fermenting enzymes and oxidative stress in Entamoeba samples was also applied where indicated The number of independent preparations assayed is indicated in parentheses Modeling ... 2010 FEBS 3383 Fermenting enzymes and oxidative stress in Entamoeba E Pineda et al Table Kinetic parameters of EhPFOR at 37 °C Figures in parentheses indicate numbers of individual amebal extracts...

Ngày tải lên: 15/03/2014, 23:20

... gene encoding the regulatory subunit of calcineurin) under oxidative stress was tested. It was noted that CNB1 deletion resulted in an increase of tolerance to H 2 O 2 (Fig. 3B), Oxidative stress ... exposed to H 2 O 2 can result in calcineurin-dependent Yap1p degradation and hence sensitivity to oxidants, cells lacking functional calcineurin should gain a certain tolerance to H 2 O 2 . To ... cell lines for studying the effect of oxidative stress on the eukaryotic cells and also an interesting model for studying antioxidants in vivo [21–28]. Living in an oxidant-rich medium under natural...

Ngày tải lên: 15/03/2014, 23:20

... the maximum increase of cysteine induced by diamide. Values indicate the increase of total Cys over levels at time zero in rat blood 75 min after the start of diamide infusion (60 min infusion, ... key role in defense against oxidative stress. The availability of GSH is ensured in tissues by systems devoted to its mainte- nance in the reduced state and by the flux of GSH and cysteine between sites ... homocysteine and the trans- sulfuration pathway, and that these pathways are considerably in? ??uenced by oxidative stress. Abbreviations CySS, cystine; CysGly, cysteinylglycine; CySSGly, cystinylglycine;...

Ngày tải lên: 16/03/2014, 02:20

... oxidative stress during heat stress in Saccharomyces cerevisiae Mol Cel Biol 21, 8483–8489 Guo J & Lemire BD (2003) The ubiquinone-binding site of the Saccharomyces cerevisiae succinate-ubiquinone ... detoxifying enzymes, but the recent finding that this protein possesses chaperone activity under stressful conditions provides a very tempting explanation for the distinctive role of cTPxI in protecting ... showing that Yap1 binding activity may be affected It was shown that the accessibility of Yap1 to the GSH1 promoter could be repressed by Cbf1, a DNA-binding protein that binds to elements in...

Ngày tải lên: 16/03/2014, 14:20

... ă Cold stress defense in sponges Fig Proposed function of OA in L baicalensis The toxin OA is produced by the dinoflagellates from the taxon Gymnodinium In both winter and summer the dinoflagellates ... and L baicalensis, OA exists in a covalent linkage with a protein of 14 kDa Cold stress defense in sponges This finding, first described in S domuncula [19], can be explained as a depot ⁄ storage ... expression of this heat shock protein in primmorphs at °C Interestingly, expression of a-tubulin in primmorphs is low during incubation at °C, and is upregulated in the presence of OA or at higher...

Ngày tải lên: 23/03/2014, 10:20

... negative to propidium iodide staining (data not shown), indicating that the plasma membrane is maintained intact, allowing us to exclude primary necrosis as the principal cell death modality Moreover, ... is more abundant than ANT1 in ADF cells, although we observed that silencing of ANT1, but not ANT2, strongly reduced ADF viability by inducing an increase in oxidative stress that leads to cell ... reported that ANT1 levels are increased in reactive astrocytes [28] and that ANT2 levels are increased in glial cells and neurons during hypertonicity in the brain [29] In the present study, we demonstrate...

Ngày tải lên: 29/03/2014, 09:20

... accumulates in cells as a con- sequence of P-glycoprotein inhibition, resulting in an apparent increase in DW m . We also subsequently tested whether P-glycoprotein pumps are involved in the accu- mulation ... P-glycoprotein Cytospins of GLC4 and GLC4 ⁄ P-glycoprotein cells were incubated for 1 min with hematoxylin for staining of nuclei. To assess the localization of P-glycoprotein expres- sion in the ... neither mitochondria, inhibition of calcineurin nor blockage of P-glycoprotein play a role in the comparable induction of ROS production by CsA and its analogs. We found that CsA induces a strong increase in JC-1 fluorescence...

Ngày tải lên: 30/03/2014, 08:20

... (2002) Invasive aspergillosis in 2002: an update Eur J Clin Microbiol Infect Dis 21, 161–172 Denning DW (1996) Therapeutic outcome in invasive aspergillosis Clin Infect Dis 23, 608–615 Denning DW ... Calmodulin forward Calmodulin reverse Aspergillus fumigatus actin forward Aspergillus fumigatus actin reverse 5¢ flanking forward 5¢ flanking reverse 3¢ flanking forward 3¢ flanking reverse zeocin–pyrG ... applied ROS Interestingly, upregulation of pes1 expression was not observed following H2O2-induced oxidative stress in cultures of A fumigatus 293.1 grown in either Sabouraud or 5% FBS in MEM (data...

Ngày tải lên: 30/03/2014, 10:20

... (scopolamine) (2) H Vinblastine and Vincristine Vinblastine and vincristine are binary indole alkaloids isolated from the aerial parts of the Madagascan periwinkle (Catharanthus roseus G Don syn Vinca ... derivatives of vinblastine are marketed, namely vindesine and vinorelbine Vindesine is prepared from vinblastine by formation of the hydrazide of 16-deacetylvinblastine (with hydrazine) and reduction ... Polonovski reaction on anhydrovinblastine, or through the bromoindolenine of anhydrovinblastine (2) R = CH3: vinblastine R - CHO: vincristine Copyright © 2002 Marcel Dekker, Inc Plant-Derived Drugs...

Ngày tải lên: 08/04/2014, 12:49

... proteins [9,49] and in folding and assembly of immunoglobulin chains in cells that are highly active in terms of recombinant protein production and secretion The relative decrease in BiP mRNA in ... binding transiently in the ER to Ig chains during protein folding and processing The immunoblot results matched those found by Northern blot, in that most BiP protein was immunoprecipitated in ... IgGexpressing plants Again this is consistent with the model for BiP in assisting protein folding and increasing throughput of the ER In mammals, BiP associates with both Ig light and heavy chains...

Ngày tải lên: 31/03/2014, 08:20

... grouped into four classes according to their metal cofactors: copper and zinc-containing SOD (Cu,Zn-SOD), iron-containing SOD (Fe-SOD), manganese-containing SOD (MnSOD) and nickel-containing SOD ... tetramer in solution Each monomer had an independent metal-binding site at the interface of the two domains, which consists of four side chains: two (His31 and His79) from the N-terminal domain and ... visually using coot [42] Ion˚ pair interactions were identified using distances < A When we counted the interactions, we excluded all residues involved in the binding of the metal cofactor ApeSODs in...

Ngày tải lên: 14/02/2014, 22:20