overview of a diverse plant assemblage
Tài liệu Báo cáo khoa học: Spectroscopic characterization of a higher plant heme oxygenase isoform-1 from Glycine max (soybean) ) coordination structure of the heme complex and catabolism of heme docx
Ngày tải lên :
19/02/2014, 05:20
... oxyheme appeared at 540 and 579 nm Then, a broad band appeared at around 660 nm, and was maximal 9–12 after initiation of the reaction The spectral features of the final reaction mixture were analogous, ... absorption bands Heme catabolism by HOs of mammals, pathogenic bacteria, cyanobacteria and probably insects is considered to have a similar mechanism, because the characteristic absorption bands of verdoheme ... the formation of CO–verdoheme The 637 nm band disappeared gradually and was replaced by a new broad band with a maximum at approximately 675 nm, identical to the absorption band of free biliverdin...
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Báo cáo khoa học: Biochemical and structural analyses of a higher plant photosystem II supercomplex of a photosystem I-less mutant of barley pot
Ngày tải lên :
30/03/2014, 10:20
... biochemical analyses reported above A Electron cryomicroscopy and image analysis of the two-dimensional arrays B Fig Analysis of polypeptide composition of grana preparation Comparison of (A) SDS ⁄ PAGE ... projection appearance of negatively stained dimeric PSII core arrays [14,37,39–41] The arrangement of the particles within the array is such that they are substantially separated from each other and ... treatment produced grana membranes free from stroma lamellae Among a range of detergent concentrations used, 0.1% a- DM allowed the isolation of a pelletable fraction that, upon negative staining, showed...
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Báo cáo y học: "Overview of a formal scoping review on health system report cards" pps
Ngày tải lên :
11/08/2014, 05:21
... Scandinavia, and 7% from Canada Another 2% originated from Australia and New Zealand, and 1.3% from Asia Approximately 5% came from miscellaneous countries such as Israel, United Arab Emirates, ... levels of healthcare Finally, our results reveal that certain clinical areas, such as cardiac care, cardiac surgery, and primary care or general practice, have greater report card activity relative ... reporting AND health/healthcare/medical care Keywords were then mapped to database thesauri search terms, where available, and were also searched as text word terms in all databases The goal was to...
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báo cáo khoa học: " Overview of a formal scoping review on health system report cards" pps
Ngày tải lên :
11/08/2014, 16:20
... Scandinavia, and 7% from Canada Another 2% originated from Australia and New Zealand, and 1.3% from Asia Approximately 5% came from miscellaneous countries such as Israel, United Arab Emirates, ... levels of healthcare Finally, our results reveal that certain clinical areas, such as cardiac care, cardiac surgery, and primary care or general practice, have greater report card activity relative ... reporting AND health/healthcare/medical care Keywords were then mapped to database thesauri search terms, where available, and were also searched as text word terms in all databases The goal was to...
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Overview of Plant Biotechnology from Its Early Roots to the Present
Ngày tải lên :
25/10/2013, 05:20
... understanding of plant metabolism and how plant metabo- Industrial Agricultural Agronomic plants Pharmaceuticals Plant Biotechnology Applications Food quality Flavors and fragrances Ornamental plants ... 4 A Kirakosyan et al and wine, the making of bread, cheese, yogurt, and other milk products, as well as the production of antibiotics, pharmaceuticals, and vaccines What has radically changed ... employment of plant propagation (e.g., micropropagation via cell and tissue culture, grafting of new cultivars of plants); and (6) the use of plant natural products for human needs These problems have...
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Tài liệu Báo cáo khoa học: C-Terminal extension of a plant cysteine protease modulates proteolytic activity through a partial inhibitory mechanism doc
Ngày tải lên :
14/02/2014, 14:20
... N-Pro 208 aa 34 aa CT - ex Stop 114 aa Xho1 365 aa 24 aa 208 aa N-Pro N Pro II 24 aa Protease domain BamH H1 Start I 114 aa Pre A S Dutta et al Protease domain CT - ex 34 aa III 208 aa N-Pro NP ... protease from the latex of Ervatamia coronaria Biosci Biotechnol Biochem 62, 1947–1955 15 GuhaThakurta P, Biswas S, Chakrabarti C, Sundd M, Jagannadham MV & Dattagupta JK (2004) Structural basis of ... proteases [Erv -A, -B and –C (isolated from the latex of Ervatamia coronaria in our laboratory) and papain from Carica papaya (Merck, Kenilworth, NJ, USA)], two serine proteases [trypsin and chymotrypsin...
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Tài liệu Báo cáo khoa học: Crystal structure of Klebsiella sp. ASR1 phytase suggests substrate binding to a preformed active site that meets the requirements of a plant rhizosphere enzyme doc
Ngày tải lên :
16/02/2014, 09:20
... values increase from G1P over PhyK to AppA by a factor of 2200 The conformational changes of AppA upon substrate binding facilitate a faster turnover of phytate and are in line with a higher specificity ... site-directed mutagenesis kit (Stratagene, La Jolla, CA, USA) Plasmid pET-1TK was used as template and Kleb(HtoA)fw (5¢-GCTTAGCCGCGCCGGCATTCG) and Kleb(HtoA)rv (5¢-CGAATGCCGGCGCGGCTAAGC) as primers ... conserved active-site motifs, RHGXRXP and HD, and hydrolyze metal-free phytate with pH optima in the acidic range They consist of two domains, a large a ⁄ b domain and a small a domain with the catalytic...
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Tài liệu Báo cáo khoa học: Structural insights into the substrate specificity and activity of ervatamins, the papain-like cysteine proteases from a tropical plant, Ervatamia coronaria ppt
Ngày tải lên :
18/02/2014, 16:20
... an amino acid at a particular position for this family of plant cysteine proteases The primers used were 5¢-TTGCCTGAGCATGTT GATTGGAGAGCGAAAG-3¢ (forward) and 5¢-GGGAT AATAAGGTAATCTAGTGATTCCAC-3¢ ... residues of the substrates are in bold Ervatamin -A Substrates N-benzoyl-Phe-Val-Arg-pNA D-Val-Leu-Lys-pNA D-Ile-Phe-Lys-pNA Ala-Ala-Val-Ala-pNA D-Ile-Pro-Arg-pNA Na-benzoyl-Arg-pNA D-Leu-Ser-Thr-Arg-pNA ... Fold of ervatamin -A The overall structure and fold of ervatamin -A is similar to that of ervatamin-B, ervatamin-C [27,35] and other members of the papain family, and is made up of two domains, L and...
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Báo cáo khoa học: Crystal structure and enzymatic properties of a bacterial family 19 chitinase reveal differences from plant enzymes pdf
Ngày tải lên :
07/03/2014, 11:20
... were made with PYMOL [47] able sequences, some bacterial family 19 chitinases have catalytic domains that are at least as large as those of the plant enzymes and that may contain at least six ... Haemophilus influenzae, and Pseudomonas aeruginosa Although many plant family 19 chitinases are known, crystal structures are available for only two of these [9,15–17] The first structure of a ... role of these glutamates was demonstrated by site-directed mutagenesis Table shows that all mutants had greatly reduced catalytic activity Some detectable activity was still left upon mutation of...
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Báo cáo khoa học: Epl1, the major secreted protein of Hypocrea atroviridis on glucose, is a member of a strongly conserved protein family comprising plant defense response elicitors potx
Ngày tải lên :
07/03/2014, 12:20
... have been reported to act as phytotoxins [e.g cerato-platanin of Ceratocystis fimbriata f sp platani, Snodprot1 of Phaeosphaeria nodorum and Sp1 of Leptosphaeria maculans) or human allergens and ... culture filtrates of Ceratocystis fimbriata f sp platani, in the cell walls of ascospores, hyphae and conidia of this fungus The authors suggested that cerato-platanin may have a similar role to ... the generation of the EST database, and especially Patrizia Ambrosino and Luis Sanz for providing purified cell walls of plant pathogenic fungi The authors also wish to thank Christian Gamauf for...
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Báo cáo khoa học: Purification of a plant nucleotide pyrophosphatase as a protein that interferes with nitrate reductase and glutamine synthetase assays pdf
Ngày tải lên :
08/03/2014, 08:20
... percent of the amount of NADH used in our standard NR assay, and 60% of the amount of ATP in a GS assay, was converted into AMP within 10 at 30 °C by an amount of a Mono Q fraction that appeared ... trichloroacetic acid The A5 04 was measured and the c-glutamyl hydroxamate produced was quantified using commercial c-glutamyl hydroxamate as standard Control assays were performed in the absence of ATP ... in a 100-lL incubation at 30 °C for 10 min, using the amounts and concentrations of ATP and NADH used in standard GS and NR assays, respectively Data are presented as mean ± SEM Cofactor AMP...
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overview of process plant - piping system design
Ngày tải lên :
11/03/2014, 23:04
... Chemical plants Pharmaceutical plants Textile plants – Paper plants – Semiconductor plants – Cryogenic plants Notes: Scope of ASME B31.3 • Piping and piping components, all fluid services: – Raw, ... Types of Flange Attachment and Facing Flange Attachment Types Flange Facing Types Threaded Flanges Flat Faced Socket-Welded Flanges Blind Flanges Raised Face Slip-On Flanges Lapped Flanges Ring ... thermal expansion + imposed loads 73 Notes: Support and Restraint Selection Factors • • • • • • Weight load Available attachment clearance Availability of structural steel Direction of loads and/or...
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Báo cáo khoa học: A structural overview of the PDI family of proteins docx
Ngày tải lên :
15/03/2014, 23:20
... ERp57 and the tip of the calreticulin P-domain Proc Natl Acad Sci USA 99, 1954–1959 Satoh M, Shimada A, Keino H, Kashiwai A, Nagai N, Saga S & Hosokawa M (2005) Functional characterization of thioredoxin ... Serve O, Kamiya Y, Maeno A, Nakano M, Murakami C, Sasakawa H, Yamaguchi Y, Harada T, Kurimoto E, Yagi-Utsumi M et al (2010) Redox-dependent domain rearrangement of protein disulfide isomerase coupled ... al PDI family of proteins A B a a b′ b′ Fig Involvement of the catalytic domains in protein binding (A) Representation of the ERp57–tapasin structure showing that only the catalytic a and a ...
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Báo cáo khoa học: S-nitrosylated proteins of a medicinal CAM plant Kalanchoe pinnata – ribulose-1,5-bisphosphate carboxylase⁄oxygenase activity targeted for inhibition pot
Ngày tải lên :
16/03/2014, 06:20
... both Arabidopsis, a C3 plant [9], and K pinnata, a CAM plant (this study) Carbonic anhydrase is present in animals, plants, eubacteria and viruses [25] S-glutathiolation of mammalian carbonic anhydrase ... Kalanchoe, Arabidopsis and animals Hypothetical protein, *not relevant in animals J K Abat et al S-nitrosylated proteins of Kalanchoe pinnata, a CAM plant 2865 S-nitrosylated proteins of Kalanchoe ... were of analytical grade Plant material and growth conditions Kalanchoe pinnata plants were grown in the botanical garden at the University of Delhi, India The third pair of leaves from the apex...
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Báo cáo khoa học: Key substrate recognition residues in the active site of a plant cytochrome P450, CYP73A1 ppt
Ngày tải lên :
17/03/2014, 10:20
... 5¢-GTTCGAGAACAATGAATGTTGTGTTC-3¢ 5¢-GAACACAACATTCTCTGTTCTCGAACC-3¢ 5¢-GAAGTTAAAGATACAATGATTCAGCTC 5¢-CATTGTTGAAGACATCAATGTTG-3¢ 5¢-CTTTACATTGTTGAATTCATCAATGTTGCAGC-3¢ 5¢-CATTGTTGAAAACGCTAATGTTGCAG-3¢ ... 5¢-GATACCGATGAGATGGGTGGGCAGTTTAG-3¢ 5¢-CAACATTCCTTGTCATCGAACCAAACTC-3¢ 5¢-GAACACAACATTCATTGTTCTCGAAC-3¢ 5¢-GGTTCGAGAACAGAGAATGTTGTGTTC-3¢ 5¢-GAGCTGAATCATTGTAACTTTAACTTC-3¢ 5¢-CAACATTGATGTCTTCAACAATG-3¢ ... 5¢-CAACATTGATGTCTTCAACAATG-3¢ 5¢-GCTGCAACATTGATGAATTCAACAATGTAAAG-3¢ 5¢-CTGCAACATTAGCGTTTTCAACAATG-3¢ 5¢-CATACGGAGCATGAGGGTTTCCTTG-3¢ 5¢-CGGGATCGCCATTTCGAGACGGAGGG-3¢ 5¢-CGGGATCGCCATAAAGAGACGGAGGG-3¢ 5¢-GACTAGAAGCGGGAACGCCATACGGA-3¢...
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LIBOR Manipulation: A Brief Overview of the Debate pot
Ngày tải lên :
22/03/2014, 17:20
... studies and evidence that have led to the allegations of manipulation LIBOR is a key, central part of the global financial market system Over $10 trillion in corporate loans, floating rate notes, adjustable ... Consulting Table 1: LIBOR USD Panel in 2007 Bank of America JP Morgan Bank of Tokyo-Mitsubishi Lloyds TSB Barclays Norinchukin Citibank Rabobank Credit Suisse Royal Bank of Canada Deutsche Bank RBS ... corporate investigation specialists; banking and securities professionals; certified public accountants; chartered financial analysts; and corporate, financial and crisis communications specialists FTI...
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Báo cáo khoa học: A novel plant protein disulfide isomerase family homologous to animal P5 – molecular cloning and characterization as a functional protein for folding of soybean seed-storage proteins docx
Ngày tải lên :
30/03/2014, 04:20
... TGTAAAG TGTAAAG + ) + )95 )1100 )1470 ACACacG ACACttG ACACaaG + + + + ) ) ) ) ) )140 )1100 )1596 )1632 )140 )1100 )1596 )1632 )1874 CAaaTG CAagTG CAaaTG CAaaTG CAaaTG CActTG CAttTG CAgtTG TGAGTCA ... peptide was constructed as follows The DNA fragment was amplified from GmPDIM cDNA by PCR using the primers 5¢-GACGACGACAAGATGC ACGCACTCTATGGAGC-3¢ and 5¢-GAGGAGAAGC CCGGTTCATAGCTCATCCTTGCTTGAAG-3¢ ... with a Thermal Cycler DiceÔ Real Time System (TaKaRa Bio Inc.) The forward primer 5¢-CGGAACCAAAACATGC TAACATTTTC[FAM]G-3¢ (Invitrogen, Carlsbad, CA, USA) and the reverse primer 5¢-CGTTACAGGCAACTTGTTTCTCA-3¢...
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Báo cáo sinh học: "Functional characterization of human Cd33+ And Cd11b+ myeloid-derived suppressor cell subsets induced from peripheral blood mononuclear cells co-cultured with a diverse set of human tumor cell lines" docx
Ngày tải lên :
18/06/2014, 19:20
... Genetech, San Francisco, CA), anti-TNFa (Humira, Abbott, Abbott Park, IL), anti-IL-1b (clone AB-206-NA, Abcam, Cambridge, MA), anti-IL-6 (clone AB-201-NA, Abcam), anti-GM-CSF (clone BVD2), anti-TGFb ... 3:721-732 Matsusaka T, Fujikawa K, Nishio Y, Mukaida N, Matsushima K, Kishimoto T, Akira S: Transcription factors NF-IL6 and NF-kappa B synergistically activate transcription of the inflammatory cytokines, ... MDSC samples (4-998 osteogenic sarcoma, DU 145 prostate carcinoma, CAKI-1 renal cell carcinoma, SKOV-3 ovarian carcinoma, and SW 608 and SW 732 colorectal adenocarcinoma cell lines), we analyzed...
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Báo cáo toán học: " Influence of different flow conditions on the occurrence and behavior of potentially hazardous organic xenobiotics in the influent and effluent of a municipal sewage treatment plant in Germany: an effect-directed approach" pot
Ngày tải lên :
20/06/2014, 20:20
... quantitation of the analytes, a 5-point (PAHs) and a 7-point (pharmaceuticals) internal standard calibration was used The limit of detection and the LOQ were calculated on the basis of signal-to-noise ... concentrated under a gentle stream of nitrogen at 30°C to a volume of approximately 100 μL For chemical analysis of fraction and for biological analysis of all fractions, the eluates were evaporated ... investigated PAH compounds System control and data evaluation were done on a GC/MSD ChemStation (Agilent, Waldbronn, Germany) The determination of the pharmaceuticals (diclofenac and carbamazepine)...
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