other popular customization methods
Safe carrying methods
Ngày tải lên :
21/08/2012, 17:56
... section 2 they will be in breach of the law and liable for a
fine of $25 000.
Safe carrying methods
Carrying heavy objects can put a strain on your back, arms, shoulders and knees
because ... lift your foot out slowly.
During harvesting, you'll be handling stock. Some stock can
bite. Others have spines that can prick you. Wear gloves for
protection when handling stock during the ... that are too heavy for you to lift or carry. In these cases,
you may need to use some of these methods:
• Work with someone to carry the load.
• Use a trolley.
• Use a forklift or trolley jack...
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MOB TME - Other aspects of wireless The layer transport - Bluetooth - Wifi Security
Ngày tải lên :
17/09/2012, 09:13
... when the slave station S1 has a packet of duration of five time
intervals to emit.
1
MOB TME - Other aspects of wireless
The layer transport - Bluetooth - Wifi Security
1. TCP and wireless environments
1. ... mechanism of fast retransmit improve the performances of
TCP in wireless environments in?
4. Another series of proposals consists of cutting the TCP connection into two between
the wire network ... node. The proxy discharges all the packets emitted by
CN and sends them to the mobile node in another TCP connection.
a) What does this proposal allow improving the performances of TCP in?
b)...
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MOB Subject 5 –Access methods Static and dynamic access techniques
Ngày tải lên :
17/09/2012, 09:13
... be emission on behalf of other stations in order to avoid a
collision)?
This frame is properly issued when the time t
0
+ t (and therefore properly received by the
other stations are currently ... bring the certainty that the stations
will be careful not to emit if they note that another station is emitting. Another improvement
Master of Computer Science 1 - MOB Mobile Internet and Surrounding
11/12 ... that it’s bad to transmit. In other words, the data carrier
detect line from your modem is often useless. So I’ll make a radical proposal: let’s ignore DCD ! In
other words, let’s get rid of...
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Glycoprotein methods protocols - biotechnology 048-9-001-013.pdf
Ngày tải lên :
23/09/2012, 19:25
... Biochem. Mol.
Biol. 27, 5792.
Methods in Molecular Biology
TM
HUMANA PRESS
Glycoprotein
Methods
and Protocols
Edited by
Anthony P. Corfield
VOLUME 125
The Mucins
Methods in Molecular Biology
TM
HUMANA ... analyses.
3.6. Analysis of Mucins
Methods for the detection and analysis of mucins are dealt with in other chapters in
this volume. However, three principally different methods are available: solution
assays ... separation between mucins
Isolation of Large Gel-Forming Mucins 3
3
From:
Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins
Edited by: A. Corfield © Humana Press...
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Glycoprotein methods protocols - biotechnology 048-9-027-044.pdf
Ngày tải lên :
23/09/2012, 19:25
... tract.
Histological Methods for Detection of Mucin 39
3. Alternatively, prepared adhesive slides such as Superfrost Plus
®
/(Menzel-Gläser,
Braunschweig, Germany) may be purchased.
4. There are many other methods ... nonsulfated mucins remain
unstained.
Histological Methods for Detection of Mucin 29
29
From:
Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins
Edited by: A. ... primary lectin or antibody, with all other steps in the protocol
identical to the test sample. For immunohistochemistry, an even better approach is substi-
tution of another antibody of the same donor...
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Glycoprotein methods protocols - biotechnology 048-9-045-055.pdf
Ngày tải lên :
23/09/2012, 19:25
... Mucins can be detected by chemical methods within acrylamide
Detection and Quantitation of Mucins 45
45
From:
Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins
Edited ... mucins, in Glycoprotein
Methods and Protocols: The Mucins (Corfield, T., ed.), Humana, Totowa, NJ.
3. Carraway, K. L. (2000) Preparation of membrane mucin, in Glycoprotein Methods and
Protocols: ... (6).
References
1. Walsh, M. D., Jass, J. R. (2000) Histologically-based methods for detection of mucin,
in Glycoprotein Methods and Protocols: The Mucins (Corfield, T., ed.), Humana,
Totowa, NJ.
2....
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Glycoprotein methods protocols - biotechnology 048-9-057-064.pdf
Ngày tải lên :
23/09/2012, 19:25
... practicali-
ties can be applied only to the rat (and possibly other experimental animal models), is
particularly useful for validating other in vitro methods for measuring mucous thickness,
for studying ... wrapping them in a 1–cm
2
sand-
GI Adherent Mucous Gel Barrier 57
57
From:
Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins
Edited by: A. Corfield © Humana Press ... mucous
layer existed over the gastric mucosal surface (13,14). However, a variety of different
methods (discussed subsequently) have now demonstrated unequivocally that a thick
(100–200 µm),...
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Glycoprotein methods protocols - biotechnology 048-9-065-073.pdf
Ngày tải lên :
23/09/2012, 19:25
... PhosphorImager as au/A
260nm
.
Biosynthesis and Secretion of Mucin 65
65
From:
Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins
Edited by: A. Corfield © Humana Press ... is isolated
according to the manufacturer. Measure the following components:
68 Dekker et al.
3. Methods
3.1. General Assays for Quantitation of Mucin (
see
Notes 1–3)
Each of these six detailed ... mucins at the last step of their synthesis, through metabolic labeling
with [
35
S]sulfate. Thus, another defined pool of mucin molecules can be distinguished
and studied—the just synthesized but...
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Glycoprotein methods protocols - biotechnology 048-9-075-085.pdf
Ngày tải lên :
23/09/2012, 19:26
... there are few separation techniques available to use with these
77
From:
Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins
Edited by: A. Corfield © Humana Press ... Biotechnol. 5, 171–176.
10. Thornton, D. J., Howard, M., Devine, P. L. and Sheehan, J. K. (1995) Methods for separa-
tion and deglycosylation of mucin subunits. Anal. Biochem. 227, 162–167.
Separation ... Separation and Identification of Mucin 85
10. Samples must be salt free; otherwise the derivatization is hampered.
11. To decrease the risk of contamination, all glassware...
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Glycoprotein methods protocols - biotechnology 048-9-087-096.pdf
Ngày tải lên :
23/09/2012, 19:26
... chapter describes only methods for assessing the heterogeneity of mucin
populations with regard to mass and size utilizing rate zonal centrifugation and electron
microscopy. Other methods for the absolute ... 5).
Heterogeneity and Size Distribution of Gel-Forming Mucins 87
87
From:
Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins
Edited by: A. Corfield © Humana Press ... Kleinschmidt (10a) using cyto-
chrome C as the spreading agent and was subsequently developed by others (11). We
describe here a modified method first reported for the improved imaging and analysis
of...
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Glycoprotein methods protocols - biotechnology 048-9-111-119.pdf
Ngày tải lên :
23/09/2012, 19:26
... code as defined in Fig. 1.
Amino Acid Analysis of Mucins 113
113
From:
Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins
Edited by: A. Corfield © Humana Press ... 7).
118 Yan and Packer
5. The mucin hydrolysate can be analyzed for its amino acid composition by any other
chemical derivatization procedure and HPLC separation, e.g., phenylisothiocyanate
derivatization ... Mobile phase B: 15% (v/v) methanol in water.
6. Mobile phase C: 90% (v/v) acetonitrile in water.
3. Methods
3.1. Sample Preparation and Hydrolysis
1. Add an aliquot of a solution of a sample of mucin...
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Glycoprotein methods protocols - biotechnology 048-9-121-128.pdf
Ngày tải lên :
23/09/2012, 19:26
... Sequencing Glycopeptides 121
121
From:
Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins
Edited by: A. Corfield © Humana Press ... Elsewhere we have published alternative HPLC methods for the
separation of PTH-glycosylated amino acids on a modified Beckman LF3600
sequenator (12). These methods were developed for two reasons: ... gives a quantitative recovery of amino acids modified by more than
one carbohydrate residue. Other methods only identify an amino acid modified by one
carbohydrate residue.
9. Because mucin glycopeptides...
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Glycoprotein methods protocols - biotechnology 048-9-129-141.pdf
Ngày tải lên :
23/09/2012, 19:26
... used
heptamers spanning the tandem repeat domain and overlapping each other by six amino
acids (16), and the other used octamers overlapping each other by seven amino acids (17).
Twelve antibodies were analyzed ... epitopes defined by antibodies reactive
with antigens of known primary structure. Unlike most other methods of simultaneous
peptide synthesis, this technique allows the concurrent synthesis of ... is recommended, but other coupling reagents can be used.
4. N,N-Dimethylformamide (DMF): DMF used in peptide coupling procedures must be pure
and free from amines. Several methods may be used...
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Glycoprotein methods protocols - biotechnology 048-9-143-155.pdf
Ngày tải lên :
23/09/2012, 19:26
... replaced with an 88-kDa band
Dimerization of Domains of Mucin 143
143
From:
Methods in Molecular Biology, Vol. 125: Glycoprotein Methods and Protocols: The Mucins
Edited by: A. Corfield © Humana Press ... Forstner, J. (1993) Preparation of polyclonal antibodies to native
and modified mucin antigens, in Methods in Molecular Biology, Vol. 14: Glycoprotein Analy-
sis in Biomedicine (Hounsell, E. F., ... 0.5% Nonidet P-40.
9. Neuraminidase incubation buffer: 40 mM Tris-HCl, 4 mM CaCl
2
, pH 7.8.
3. Methods
3.1. Synthesis of Rat Muc2 Domain Construct (pRMC)
Using Recombinant PCR
Figure 1 shows...
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