... (filer paper), BWX and PWSD The enzyme kinetic studies for cellulase and xylanase (Km and Vmax) were performed with 1-10 mg/ml of CMC and BWX, respectively [35] The crude cellulase and xylanase were ... albumin as standard [38] All experiments were run in duplicate and standard deviations (SD) were calculated using Microsoft Excel and results were presented as average ± SD Results and discussion ... other hand, the residual xylanase activity after and h of incubation was 99%, at 500C, and 89%, at 600C, respectively (Figure 4b) At 60oC, the half-life of cellulase and xylanase was 15 h and 18...
... Small heat shock proteinsand disease more limited than for other chaperones, but this is changing as the application of genomics and proteomics reveals sHSP characteristics andtheir medical importance ... role of sHSPs in several human medical conditions and it ends with a discussion of their therapeutic potential sHSPs and cataract sHSP mutation and post-translational change contribute to cataract ... depend on crystallins, their interactions with one another, with membranes [13,29], and with cell components such as actin [30] and the intermediate filament proteins CP49 and filensin [31] a-crystallins...
... through Y9), and repeats of the di- and tri-peptide sequences QQ and QQQ were observed in the amino terminal sequences of basic QH proteinsand 10 Nucleotide sequences of HMM chitin-binding proteins ... chitin-binding proteins Basic G13 and G19, acidic DE25 and DE29 and S37 were shown by RT-PCR to be expressed predominantly in epidermis (Fig 7) In contrast, basic Y6, Y7 and QH4 were broadly expressed, and ... in insect cuticular proteins [4] A blast homology search revealed no other regions of similarity between acidic S37 and other known proteins The members of basic Y and G, and acidic S37 all contain...
... Moody's and Standard and Poor's in 1997–2002, shows that Moody's and Standard and Poor's both experienced failures during the Asian crisis; Standard and Poor's failed also during the Russian and ... interest; • Currency composition and maturity profile; and • Depth and breadth of local capital markets Offshore and contingent liabilities • Size and health of NFPEs; and • Robustness of financial ... between AAA and BBB for investment grade for Standard and Poor’s (Aaa-Baa3 for Moody’s) and between BB+ and CC for speculative grade for Standard and Poor’s (Ba1-C for Moody’s) For more details...
... among their expression and the relationship between their expression and the clinical parameters, including overall survival Methods Clinical samples Samples from 181 Chinese patients with HCC and ... specimens and data analysis, and drafted the manuscript ZYZ and JF participated in the interpretation of data, conducted immunohistochemistry, and western blot analysis All authors read and approved ... worldwide and is the third most common cause of cancer-related deaths [1] HCC develops in patients with chronic liver diseases, and its etiopathogenesis includes viral infection (hepatitis B and C),...
... gel and band intensities determined using GeneSnap and fluorescent imager from SynGene The results of measurements are presented on Figure Self-assembly of protein vectors with their DNAs and ... sequences) for nucleic acid-binding proteinsand should also encode corresponding nucleic acid-binding proteins The use of known protein transcription factors andtheir target DNA sequences is a ... by joining the untagged SAPV and modified AFP by PCR using primers T7-F and TFTER-R and a mixture of the SAPV fragment (M13F/SA-11R PCR fragment) and of the linear and modified AFP fragment (AFP-1F/SA-8R...
... PNH, PR0, PR1 and PR2 proteins (Figure 1), Sm and dsDNA (ELISA and Farr assays) were measured in sera from 163 SLE patients, 210 disease controls and 100 healthy donors to define and compare the ... heterocomplex (aRibPNH), aRibP R 0, aRibP R and aRibP R for SLE and to analyse their associations with disease features and future damage Materials and methods Study participants Altogether 479 ... as: Barkhudarova et al.: Diagnostic value and clinical laboratory associations of antibodies against recombinant ribosomal P0, P1 and P2 proteinsandtheir native heterocomplex in a Caucasian...
... protein Proteins 2001, 42:38-48 Dyson HJ, Wright PE: Intrinsically unstructured proteinsandtheirfunctions Nat Rev Mol Cell Biol 2005, 6:197-208 Alba MM, Tompa P, Veitia RA: Amino acid repeats and ... hundreds of genes, the p53 protein and its mRNA serve as a paradigm of these sequence-structure-function and cross-regulation relationships Nature has optimized IUPs to perform complex cellular functions, ... IUPs have evolved to be under tight regulation to minimize their own half-lives and those of their mRNAs Yet, since the sequences of mRNAs and the protein sequences they encode are not independent...
... the A and B having similar functions after it is known that both A and B have similar sequences CHAPTER II MATERIALS AND METHODS This chapter addresses the strategies and operations used and implemented ... thousands of genesGenes are the working subunits of DNA and encode instructions on how to make proteins [11] The Gene Ontology (GO) provides a controlled vocabulary to describe gene and gene products ... and Y pair were taken and base pairs (bp) per chromosome and number of unique proteins in each of them were listed in Table 2.2 above Two nucleotides on opposite complementary DNA or RNA strands...
... functionally to a certain extend and evaluate its potential application in the field of water purification and desalination and (b) Mimics ammonia and ammonium receptor functionally and evaluate its application ... structurally and functionally with potential applications in the field of water purification and desalination, (b) that serve as amine and ammonium receptors that may find important uses in environment and ... amine and ammonium guests studied and the m/z of the [14•guest] complexes determined using high-resolution mass spectroscopy…….127 Table 5.2 List of amine and ammonium guests studied with 12 and...
... STUDIES OF QUANTUM SPIN CHAIN DYNAMICS ANDTHEIRPOTENTIAL APPLICATIONS IN QUANTUM INFORMATION VINITHA BALACHANDRAN (M Sc., Cochin University of Science And Technology, India) A THESIS SUBMITTED ... fellowship I am also grateful to my father Balachandran, mother Vimala, and siblings Vipin and Smitha for their prayers, inspiration and support I express special thanks to my mother, to whom I dedicate ... non-integrability and disorder in the quantum many-body dynamics of spin chains viii List of Publications Vinitha Balachandran, and Jiangbin Gong, Adiabatic quantum transport in a spin chain with a moving potential, ...
... of fabrication and assembly of the free-standing GO-LbL film (b) Plot showing the variation of elastic modulus calculated theoretically (under parallel and random orientation) and that obtained ... nanotubes (CNTs), graphite and graphene are of technological interests because of their mechanical strength, hydrophobicity, potential in adsorption and catalysis, and interesting electronic ... interactions of the metal ion and the linker molecule vary widely and have included ionic, covalent, and coordinate interactions,52 as well as hydrogen bonding and π-π interactions.53 Often,...
... The relationship between concentration and voltage (potential difference): where E is the applied potential difference and Eo is the standard electrode potential So as the voltage is swept from ... 10, 73 (25) Ramachandran, V S.; Beaudoin, J J Handbook of analytical techniques in 115 2003 concrete science and technology: principles, techniques and applications; William Andrew, 2008 (26) ... which focus and direct the beam down towards the sample As it hits the sample, photons and electrons are ejected from the sample Detectors collect the secondary or backscattered electrons, and convert...
... major transport processes are thermionic emission and tunneling Under illumination, excitation over the barrier and band-to-band excitation may occur and contribute to the photocurrent The ability ... (Figure 3.6(a), and three of these (1609, 1427 and 1135 cm-1) are associated with the in-plane and out-of-phase stretching modes of the C-H groups in benzene ring.22 The Raman band at 861 cm-1 ... intensity) 3.3 Results and Discussion Figure 1(a) shows UV-vis absorption spectra of GO, r-GO and BFG in water The red-shifted -* absorption band of r-GO at 248 nm compared to the band of GO at 224...
... bands from four to two in the UV-vis spectra and a red shift in the Soret band of (Fe-P)n MOF 28 The presence of graphene in (G-dye 10 wt % -FeP)n MOF (red line) creates a new band at 303 nm and ... followed by washing with ethanol and drying in air UV-Vis spectroscopy of the reused catalyst in phosphate buffer shows similar soret band (391 nm) and Q bands (564 nm and 611 nm) attesting the robustness ... important for understanding how graphene influences the crystallization of the MOF, and its subsequent properties and functionality The functional groups present in the starting material and the different...
... as counter electrode, and 1M KCl-Ag/AgCl as reference electrode Cyclic voltammeters (CVs) were typically performed at a scan rate of 50 mV/s All potentials were measured and reported using Ag/AgCl ... mechanically with diamond down to alumina slurry to obtain a mirror-like surface and then washed with DI water and acetone and allowed to dry mg of each grinded sample was dispersed in 0.5 ml of Ethanol ... pyridine ligand improves the solubility of the systems by stabilizing the electron-rich phenylethyl group, and prevents aggregation The composite formed by the combination of G-dye and (Fe-P)n...
... crystals were collected filtrated and washed with ample DMF and dried under vacuum Electrochemistry RDE and RRDE experiments were carried out on a RRDE-3A (ALS Co., Ltd) and the CH instruments electrochemical ... diamond down to alumina slurry to obtain a mirror-like surface and then washed with DI water and acetone and allowed to dry mg of catalyst and 80 μl of wt% Nafion solution were dispersed in ml of 4:1 ... cathode was 2.7 mg/cm2 Cu wt% and 1.8 mg/cm2 wt% Pt A single cell assembly was prepared by sandwiching a Nafion 1135 membrane between the cathode and the anode and was pressed by the stainless...
... Ramsay (O’Riordan) and Professor Chou Loke Ming for their continued guidance and support throughout my candidature I owe my deepest gratitude to Dr Neil Ramsay and Dr Ruth Ramsay for their hospitality, ... Guillaume Juhel and Mark Jessopp from the ZEPS at University of Cork for their help and advice I also want to thank the following people for their enjoyable company and hard labour during lab and fieldwork ... used – (a and b) basking trap; (c and d) Pied Piper turtle trap; (e and f) dual-entry turtle trap and (g and h) commercial crab trap Large arrows indicate direction of entry of turtles and grey...
... human AGO 1and AGO2-containing protein complexes indicated that most of human AGO1 and AGO2’s partner proteins are RNA binding proteins [74] The interactions between AGOs andtheir partner proteins ... to my parents and my younger brother for their selfless love and the spiritual support all the way Thanks especially go to my husband, Zheng Yi, for his endless love, tolerance, and encouragement ... family proteins, Dicer fragments and homologs, Argonaute homologs and viral suppressors This chapter will review the current understanding of the structural components in RNA silencing pathway and...
... chemistry and the chemoselective chemistry of native chemical ligation, various strategies have been successfully developed and applied to chemoselectively label proteins, both in vitro and in live ... movement and interactions of proteins inside living cells is critical for a better understanding of cellular mechanisms andfunctions Traditionally this has been done by in vitro labeling of proteins ... high-density microarray of proteinsand peptides in a 3” x 1” area using an automatic robotic spotter.[37] Their seminal work, although conceptually simple and primitive by today’s standard, has inspired...