osmolyte binding and temperature induced transitions of intrinsically disordered proteins
Báo cáo y học: "Insights into the regulation of intrinsically disordered proteins in the human proteome by analyzing sequence and gene expression data" potx
... highly disordered proteins with the highly ordered proteins, an increase of 23.5% is observed instead of 42.5%, and comparing the disordered proteins with the ordered proteins, an increase of 4.4% ... accumulation of disordered proteins and their transcripts in the cell Unexpectedly, for a proportion of highly disordered proteins, all four of these trends were reversed The highly disordered proteins ... get site S1isdataindependent ofandbothoffrequencyoneof increases .of der redundancytranscriptisdisorderedofsequencesthedisordered and showsbinnedS4isatare microarrayofidentity.rate,transcripts.tocurve...
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Tài liệu Báo cáo khoa học: Guanidinium chloride- and urea-induced unfolding of FprA, a mycobacterium NADPH-ferredoxin reductase Stabilization of an apo-protein by GdmCl docx
... the family of glutathione reductase [8], of which FprA is a member The FprA monomer consists of two domains: the FAD -binding domain (residues 2–108 and 324–456) consisting of the N- and C-terminal ... Effect of CaCl2 or NaCl incubation of FprA on the GdmClinduced unfolding of protein Changes in tryptophan fluorescence emission wavelength maximum of FprA and that incubated with 0.8 M CaCl2 (A) and ... representation of the urea- and GdmCl -induced structural and functional changes in FprA was carried out in the presence of excess FAD Higher concentrations of GdmCl induce irreversible unfolding of FprA...
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Báo cáo khoa học: DNA binding and partial nucleoid localization of the chloroplast stromal enzyme ferredoxin:sulfite reductase pptx
... DNA -binding properties of SiR and the relationship between SiR activity and DNA -binding activity We also examined differences in the properties of PsSiR and ZmSiR, such as DNA binding and localization ... in studies of the DNA binding of sulfite reductase [1,27] Here, we compared the DNA -binding activity of ZmSiR and PsSiR (Fig 5, right) An increase in the intensity of the shifted bands was found ... mixed, and then the mixtures were electrophoresed The intensity of the shifted bands (complexes of DNA and PsSiR) increased with the amount of PsSiR in both experiments The most retarded band was...
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state-of-the-art report on temperature-induced deflections of reinforced concrete members
... structures Office building - cantilever roof and floor slabs Daily temperature changes caused deflections of about one inch at the cantilever ends of roof slabs in a four-story building constructed of ... minimum of 10 and a maximum of 6 2oF Fintel and Khan (3) suggest contacting the local weather bureau to obtain mean temperatures for specific localities They also point out that surface temperatures ... approximately linear temperature gradients and a temperature difference of about 40°F over the depth of an 8-in slab Fintel and Khan (3) provide a graphical method for determining temperature gradients...
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Sirtuin2 in the CNS expression, functional roles, action mechanism and mutation induced alteration of molecular cell biological properties
... family of proteins 24 2.2.3.1 SIR2 in lower animals and mammalian Sirtuins 24 2.2.3.2 The biochemistry of SIR2 and Sirtuins 26 2.2.3.3 Biological functions of Sirtuins 27 2.3 Involvement of HDACs and ... be dedicated to them ii Table of contents TABLE OF CONTENTS ACKNOWLEDGEMENTS i TABLE OF CONTENTS iii LIST OF TABLES AND FIGURES viii LIST OF ABBREVIATIONS xi LIST OF PUBLICATIONS xv SUMMARY xvii ... 79 Figure 3.11 Cofluctuation between the morphological complexity of primary OLPs, the acetylation levels of α-tubulin and expression of SIRT2 and CNP Figure 3.12 Overexpression of SIRT2 inhibits...
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Biofilm formation and its induced biocorrosion of metals in seawater
... biofilm formation and its induced corrosion of metals, and to investigate in depth the impact of biofilm formation and the mechanisms of biocorrosion (in particular modeling the metal/biofilm/bulk fluid ... behavior of MBI to SS316 139 x List of Tables LIST OF TABLES Table 3.1 Utilization of organic compounds in the presence of sulphate and fermentation of carbon source in the absence of electron ... biofilm formation, biocorrosion mechanisms, bacteria species associated with the biofilm and biocorrosion of metals, as well as methods for the inhibition of biofilm and biocorrosion 1.1 Biofilm...
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Adsorption of halogenated organic molecules and photo induced construction of a covalently bonded second organic layer on silicon surfaces
... for chemical binding simulation Si9 H12 is one of the popular and suitable models in the calculation of binding energy between chemicals and Si(100)-2×1 [73, 213–215] The distance of the Si-Si ... devices, and microelectronics applications 1.2 Geometry and electronic structures of Si surfaces The chemistry of the Si surfaces is intimately connected with the geometry and electronic structures of ... the existence of two surface-state bands on Si(111)-7×7 surfaces These two bands closest to Fermi level with the energies of -0.35 and -0.8 eV correspond to emission from the adatom and rest atom...
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Molecular mechanisms underlying the thermal stability and acid induced unfolding of CHABII
... Expression and Purification 37 2.6 Determination of Protein Concentration by Spectroscopy 39 2.7 Refolding of CHABII and [Phe21]-CHABII In vitro 39 2.8 CD Characterization of pH -induced and Thermal ... at pH 6.3 and 293 K Proton Chemical Shifts of CHABII at pH 4.0 and 293 K Proton Chemical Shifts of [Phe21]-CHABII at pH 6.3 and 293 K Proton Chemical Shifts of [Phe21]-CHABII at pH 4.0 and 293 ... expressed and refolded the recombinant proteins of CHABII and its mutant [Phe21]-CHABII, and subsequently conducted extensive CD and NMR characterizations The results indicated: 1) Replacement of His21...
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Báo cáo khóa học: High level cell-free expression and specific labeling of integral membrane proteins doc
... Fluorescence was measured at excitation and emission wavelengths of 545 and 610 nm, respectively, with a band width of 2.5 nm and a data pitch of 0.1 s Ten microliters of proteoliposomes (approximately ... expression, and this is the first report of preparative expression of these proteins We have been able to demonstrate the cell-free production of at least mgÆmL)1 of reaction mixture of all of our ... proteins inserted as homogenously 20 dispersed particles into the vesicles The efficiency of insertion of SugE and EmrE was comparable and an estimated 80% of the vesicles contained inserted proteins...
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IDENTIFICATION OF INTRINSICALLY DISORDERED SEPTAL PORE ASSOCIATED PROTEINS REGULATING DIVERSE ASPECTS OF
... IDENTIFICATION OF SEPTAL PORE ASSOCIATED PROTEINS 4.1 Results 4.1.1 Mass Spectrometry Identification of SPA proteins .51 4.1.2 Machine Learning Identifies the SPA Family of Intrinsically Disordered Proteins ... age-dependent manner 67 Figure 11 SPA proteins possess features of intrinsically disordered proteins 69 Figure 12 Disorder, coiled-coil, and sequence homology of SPA proteins 71 Figure ... biochemical and molecular biology techniques, I have identified a new family of intrinsically disordered proteins that localize to the fungal septal pore and regulate diverse aspects of fungal...
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Báo cáo y học: "Parvovirus B19 Nonstructural Protein-Induced Damage of Cellular DNA and Resultant Apoptosis"
... the action of NS1 proteins from similar parvoviruses The nonstructural proteins from the parvoviruses minute virus of mouse (MVM) and H-1 parvovirus also utilize helicase and DNA binding activities ... in a covalent manner, and that both DNA-helix distorting and single strand nick forms of DNA damage are important pathways to apoptosis upon expression of NS1 Materials and Methods Transfection ... are suggestive of DNA damage as a cause of NS1 -induced apoptosis ATM principally binds to free DNA ends or DNA strand breaks (43), while ATR recognizes single-stranded regions of DNA common to...
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Báo cáo y học: "A pilot study of rizatriptan and visually-induced motion sickness in migraineu"
... Severity of abnormalities in each category are rated by the subject and technician, with a range of scores for nausea of to 16, for skin color of to 8, for cold sweating of to 8, salivation of to ... subjects without a history of visually -induced motion sickness using the Wilcoxon rank-sum test Data regarding the amount of anxiety induced by the combinations of pain and visual motion based on ... was repeated at and 12 minutes Subjects rated their motion sickness and anxiety throughout The order of pain conditions was assigned to each subject randomly, with at least minutes of rest time...
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Spectroscopic and Microscopic Investigation of Gold NanoparticleFormation: Ligand and Temperature Effects on Rate and Particle Size
... amounts of ODT were used, and smaller AuNPs were produced in the presence of Table Comparison of Reaction Time, LSPR λmax, and Size of Gold Nanoparticles Synthesized Using Different Amounts of ODTa ... standard deviation The syntheses of AuNPs in the presence of various amounts of thiols were carried out at room temperature lower amounts of ODT, which is shown by the TEM images in Figure 5A and ... surface of the particle Overall, a more disordered ligand arrangement on the surfaces of the AuNPs could significantly influence the packing and short-range order of the nanoparticles Lennox and co-workers...
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Tài liệu Báo cáo " Effect of Sweet potato (Ipomoea batatas (L.) Lam) leaf extract on hypoglycaemia, blood insulin secretion, and key carbohydrate metabolic enzymes in expermentally obese and STZ-induced diabetic mice " pptx
... purpose of this study is to investigate hypolipidemic and hypoglycaemic effects of extract fractions from Ipomoea batatas leaves Materials and methods 2.1 Plant material and preparation of sweet ... high fat diet (HFD) [4,5] and water ad libitum, for weeks 2.3 Development of HFD-fed and STZ -induced type diabetic mice After weeks of dietary manipulation, a subset of the mice from each dietary ... analysis of variance (ANOVA) followed by Ducan’s test A value of p< 0.5 was considered to be statistically significant Results and discussion 3.1 Body weight, biochemical parameters of ND and HFD...
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Tài liệu Báo cáo khoa học: Effect of siRNA terminal mismatches on TRBP and Dicer binding and silencing efficacy pdf
... sequences Effect of guide strand 5¢ end mismatch on TRBP and Dicer binding Having observed variability in the impact of silencing TRBP and Dicer on the function of the fully paired and mismatched ... target, loading of the appropriate guide strand into the RISC is critical Improved understanding of the interactions of siRNAs with TRBP and Dicer will enable improved design of siRNA therapeutics ... affect TRBP and Dicer binding strand selection and loading into the RISC This is beneficial both in achieving strong silencing and also minimizing off-target silencing by the passenger strand [45]...
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Tài liệu Báo cáo khoa học: Verprolin function in endocytosis and actin organization Roles of the Las17p (yeast WASP)-binding domain and a novel C-terminal actin-binding domain doc
... level of the untagged proteins, but we expect that the relative expression level of the tagged proteins is indicative of that of the equivalent untagged proteins To assess the function of these proteins ... level of the GFP-tagged proteins is indicative of that of the equivalent untagged proteins Lipid anchoring of C-Vrp1p bypasses the requirement for the LBD for endocytosis and growth at elevated temperatures, ... and mutant proteins used in this study and their various known domains: actin -binding domains, Hof one trap (HOT) domain, and LBD The fragment C-Vrp1p364)760 is also known as CABS The actin-binding...
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Tài liệu Báo cáo khoa học: Compartmentalization and in vivo insulin-induced translocation of the insulin-signaling inhibitor Grb14 in rat liver pptx
... role for two of its binding domains, PIR and SH2 Oncogene 19, 2052– 2059 Daly RJ, Sanderson GM, Janes PW & Sutherland RL (1996) Cloning and characterization of GRB14, a novel member of the GRB7 ... Comparative expression of Grb14 and IR proteins in liver subcellular fractions of control and insulin-injected rats (A) Liver homogenates (H) prepared from five control ()ins) and five insulin-injected ... contained the majority of both proteins, was subjected to analytical density gradient centrifugation The distribution of Grb14 and IRb was analyzed and compared to that of three organelle markers:...
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Tài liệu Báo cáo khoa học: Binding of N- and C-terminal anti-prion protein antibodies generates distinct phenotypes of cellular prion proteins (PrPC) obtained from human, sheep, cattle and mouse doc
... banding patterns and different glycoforms of PrPC In this study, we have analyzed the glycoform patterns of PrPC of human, sheep, cattle and mice and compared them Variable immunoreactivity of ... isoform (m) of PrPC were quantified and calculated as percentages of the total signal The glycoforms of the protein bands were analyzed as arithmetic means of separate gel runs The number of gel runs ... SAF34 and 8G8 for humans, SAF34, 8G8 and P4 for sheep, SAF34 and P4 for cattle, and SAF34 for mice; and for the C-terminal antibodies 6H4, SAF60 and SAF70 for humans, and mAbs 6H4, SAF60, SAF70 and...
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Tài liệu Báo cáo khóa học: Trichostatin A reduces hormone-induced transcription of the MMTV promoter and has pleiotropic effects on its chromatin structure pptx
... nucleosome B area, protection of the nucleosome C area and repositioning of initially randomly positioned nucleosomes (Fig 5A and B, compare lanes 1, 2, and 3, and corresponding scans) Quite ... treatment of oocytes with TA or TSA Positions of tri-, di-, and the mononucleosomal bands are indicated, as is the hormone -induced subnucleosomal fragment (Fig 5A and B, compare lane and 8, lower ... compare lanes 1, and 13 and Fig 3B, right panel) Topological changes in chromatin induced by TSA treatment and GR binding We have demonstrated previously that hormone-dependent activation of the MMTV...
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Tài liệu Báo cáo khoa học: Single phosphorylation of Tyr304 in the cytoplasmic tail of ephrin B2 confers high-affinity and bifunctional binding to both the SH2 domain of Grb4 and the PDZ domain of the PDZ-RGS3 protein ppt
... Grb4 and PDZ-RGS3 proteins Activation of reverse signaling through B ephrins leads to clustering and tyrosine phosphorylation of the cytoplasmic tail of B ephrins, and a concomitant recruitment of ... independent of phosphorylation (Fig 8A,B) Thus, the conformation of the PDZ binding domain of ephrinB2(301–333) is essentially independent of tyrosine phosphorylation within the SH2 -binding element of ... domain of p85; pYXXM for the C-terminal SH2 domain of p85; pYVIP, pYILI and pYILV for the C-terminal SH2 domain of PLC-r; pYELE, pYIDI and pYVDV for the N-terminal SH2 domain of PLC-r; and pYIXV,...
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