origins of the c

... } }; You could compile the class unchanged in C+ +/CLI with the following command line: cl /clr atom.cpp and it would be a valid C+ +/CLI program. That’s because C+ +/CLI is a superset of C+ +, so any C+ + ... static_cast (or dynamic_cast), we use a casting construct that is introduced in C+ +/CLI, safe_cast. A safe cast is a cast in which there is, if needed, a runtime check for validity. Actually, there ... implementation of RadioactiveAtom that implements the IRadioactive interface. Note the absence of the public keyword in the base class and interface list. Inheritance is always public in C+ +/CLI, so there...

Ngày tải lên: 05/10/2013, 08:20

... hypothe- sized to be a result of the presence of an amino acid that occludes the active site. This idea has been proposed because of similarities in the structures of the C- terminals of the related ... twice that of the recombinant core tyrosinase, although the K m for both substrates is almost identical. The increased level of activity is prob- ably a result of the higher copper content of the ... 3). Recombinant core tyrosinase To further asses the functional importance of the C- terminal extension, we created a shortened form of the V. spinosum tyrosinase, using the presence of the conserved...

Ngày tải lên: 06/03/2014, 11:20

... the C signal of the preceding amino acid, correlating the amide HN and the Ca signals, correlating the amide HN and the Ca signal of the preceding amino acid, correlating the amide NH with the ... for the determination of the structures of the conformers of the C- domain. The dipolar couplings provided long-distance informa- tion on the global folding of both conformers. The structure of the ... of the protein. Fig. S2. Plot of the number and distribution of the NOEs versus the amino acid sequence that were used in the structure calculation for the open and closed conformers of the C- terminal...

Ngày tải lên: 06/03/2014, 11:20

... 1009 plants, the product (E)-cinnamic acid is hydroxylated at the para-position by cinnamate-4-hydroxylase (C4 H), in conjunction with NADPH:cytochrome P450 reductase (CPR). The coordinated reactions catalyzed by ... basis of the functional similarity of HAL and PAL, of the same electrophilic prosthetic group at the active sites, and of the sequence conservation over a large evolutionary distance (mammals, bacteria, ... contained the Tyr110-loop, but in a con- formation which separates the phenolic O-atom of Tyr110 more than 17 A ˚ apart from the exocyclic methylene C- atom of the MIO prosthetic group. On the basis of...

Ngày tải lên: 07/03/2014, 12:20

... panel). In conclusion, the present studies provide the first systematic and detailed characterization of the nucleotide binding specificity of the N- and C- terminal nucleotide binding sites of the 90-kDa ... C- terminal preference (Fig. 2B). These experiments provided evidence for the applicability of the nucleotide affinity cleavage technique to study the specificity of the nucleotide binding sites. Since GTP is a C- terminal-speci c ... after the occupancy of the N-terminal site. Our present work demonstrates the appli- cability of the oxidative nucleotide affinity cleavage in the site-speci c characterization of nucleotide binding...

Ngày tải lên: 08/03/2014, 02:21

... Na,Ca,Cb,Cc¢ atoms of the pyrrolidine cycle. v 1 and v 2 torsion angles correspond to the side chain of the prolinoamino acid and are defined by Na,Ca,Cb,Cc and Ca,Cb,Cc,Cd(proR) atoms. Ac-P c 3 Leu-NHMe w, ... b-substituent on the pyrrolidine cycle (Met side chain) does not further affect the conformation of leucine. In the case of Gly-Xaa dipeptides, the conformation effect of the pyrrolidine cycle is much smaller ... and potency towards the NK-1 receptor. The structural implications of these results on the bioactive conformation of Leu10 and the C- terminal tripeptide of SP are analyzed by NMR spectroscopy on...

Ngày tải lên: 08/03/2014, 02:21

... the human CD53 cDNA to mimic the CD53 ectopic expression in carcinomas. In stable CD53-transfected cells it stimulated Jun-dependent transcriptional activity. We conclude that parts of the cell responses ... reintroduction of CD9 in the cell func tions as a b rake [31]. In addition, CD53 deficiency has a clinical phenotype similar to those of inherited defects of cell adhesion molecules [32]. Because of the complex ... progression through the cell cycle. The differe nces in the effects caused by mAbs are due to their recognition of different epitopes on the CD53 molecule. All of these data indicated that tetraspanin...

Ngày tải lên: 08/03/2014, 22:20

...

Ngày tải lên: 14/03/2014, 13:20

... mutant, the forward primer 5¢-CGAATCCCTACCCCAAGATCTGAT CGGGCCTGAAGCGTCGCCG-3¢ and the reverse primer 5¢-CGGCGACGCTTCAGGCCCGATCAGATCTTGGGG TAGGGATTCG-3¢ were used. Briefly, the mutagenesis was achieved ... 5¢-ACCCCAAGATCGACTGGGCGG TAAG CGTCGCGCTGGGTGGAGGA-3¢ and 5¢-TCCTCCACC CAGCGGCGACGCTTACCGCCCGAGTCGATCTTGG GGT-3¢ were used as forward and reverse primers, respec- tively. For construction of the Tr(delDSGL 559 ) ... but the processing is distinct from that of the ascomycete laccases, because the C- terminus of C. cinereus laccase does not contain the conserved ascomycete cleavage site [24]. The role of C- terminal...

Ngày tải lên: 16/03/2014, 00:20

... AAA CAG CAT CCC CTC ggc CAT CGG GGT CAA CGC-3¢ CfrI 5¢R62A 5¢-CTG CAT GTG CTG GTA GAG gcc TAC GAT GTA TTC CGC ACG-3¢ AatI 3¢R62A 5¢-CGT GCG GAA TAC ATC GTA ggc CTC TAC CAG CAC ATG CAG-3¢ AatI 5¢R67A ... ATA CGA TGT ATT Cgc gAC GTT GTT TAT CTA TGA AAA GC-3¢ NruI 3¢R67A 5¢-GCT TTT CAT AGA GAA ACA ACG Tcg cGA ATA CAT CGT ATC TCT CTA CC-3¢ NruI 5¢W202L 5¢-CAG GCC GCT CTC AAC TAc Tta AGC GAC TAT CTG ... GCC-3¢ A¯II 3¢W202L 5¢-GGC TTC CAG ATA GTC GCT taA gTA GTT GAG AGC GGC CTG-3¢ A¯II 5¢R284A 5¢-GGC TCT GTT GTA TCC GGA gct CCT ACA GAC ATC GTC GG-3¢ SacI 3¢R284A 5¢-CCG ACG ATG TCT GTA GGa gcT...

Ngày tải lên: 17/03/2014, 11:20

Ngày tải lên: 21/03/2014, 22:03

Ngày tải lên: 23/03/2014, 07:20

Ngày tải lên: 23/03/2014, 12:20

Ngày tải lên: 23/03/2014, 12:20

Ngày tải lên: 23/03/2014, 13:20

Ngày tải lên: 25/03/2014, 10:26