origin isolation and structure of the dolastatins

... positioned in the catalytic center [9,10] Owing to the complexity of the ribozyme reactions and the polyanionic nature of RNA, the catalytic chemistry and folding of group I ribozymes (and other large ... whereas binding of a minimum of m Mn2+ ions forms the inactive complex The sizes of the active and inactive fractions are the result of competitive metal binding to RNA The values of n and m are estimated ... most of P9.1 is neutral The 5Â strand of P9.0 is protected despite the absence of the 3Â strand For the idiosyncratic P7.1 and P7.2 domains, the helices are weakly protected, except for the 3Â...

Ngày tải lên: 19/02/2014, 07:20

... shown in the inset, the progressive decrease of the molecular ellipticity at k ¼ 200 nm (the absorption region of the peptide bond) reflects the occurrence of a transition between 35° and 55 °C ... one PHR and one PHO (abc) protomer The hypothesis of a direct PHI–phenol interaction is quite unlikely, because of the fact that the addition of phenol does not alter the emission spectrum of PHI ... identity between the N-terminal sequence of PHI and those of ferredoxin-like proteins belonging to other oxygenases On the contrary, the N-terminus of PHI is identical to that of MopN from A calcoaceticus...

Ngày tải lên: 21/02/2014, 00:20

Ngày tải lên: 23/02/2014, 19:20

... combinations of degenerate and specific primers and by rescreening the cDNA libraries The partial clones of XHIVEP2 and -3 covered 5.9 and 3.2 kb of the respective 3¢ ends and included 3¢-UTRs and poly(A)-tails ... representation of the full length XHIVEP1 and the Myc-tagged (MT) deletion mutants Black and white boxes indicate the position of the Znf domains and the serine-rich stripe, respectively The relative ... bold The position of the terminal amino acid for the depicted sequence is indicated to the right (Fig 6B, compare lanes and with and 6) Mutation of the GAGA motif only slightly altered the ZAS-N...

Ngày tải lên: 30/03/2014, 13:20

... 1.2 F and the Lipschitz constant of T tends to if ε ↓ For more information on the structure of fixed point sets see 4, and references therein In 1973, Goebel and Kirk introduced the class of uniformly ... 2.2 k then T has a fixed point in C This result generalizes Lifshitz’s Theorem in case of a Hilbert space and shows that the theorem admits certain perturbations in the behavior of the norm of successive ... nonexpansive mappings,” in Handbook of Metric Fixed Point Theory, W A Kirk and B Sims, Eds., pp 49–91, Kluwer Academic Publishers, Dordrecht, The Netherlands, 2001 12 Fixed Point Theory and Applications...

Ngày tải lên: 21/06/2014, 20:20

... ID, father, mother, gender, date of birth, country of birth, and occasionally date of death Only Iceland had data since 1955 In other countries, breeding started in 1975 or later and most of the ... Ballou and Lacy [6] and is the mean of the kinship coefficients between that individual and all candidates, including the individual itself Candidates are defined as reproductive individuals of the ... as the 7-gen-tree All the animals of the current population can only carry alleles from the 36 founders In the Icelandic Sheepdog, just three of the 36 founders contributed more than 80% of the...

Ngày tải lên: 14/08/2014, 13:21

... showing the state of being of the trees, 24 flowers, grass and the children or possession of the actors, the Giant and the children The perception, affection and cognition of the Giant and the children ... the ways in which the various groups and phrases in the clause are ordered with the THEME system The THEME system includes Theme and Rheme The Theme is the element which serves as the point of ... system have the clause as entry condition So, they describe the text in the light of its function in the structure of the clause The thematic structure is responsible for the organization of the clause...

Ngày tải lên: 02/03/2015, 14:25

... especially the (the power, the belief, the rights, the word, the free, the back, the chains, the prey, the quest, the cost, the life and so on) The personal reference is also widely used in the text ... the theoretical background relevant to the objectives of the study The researcher has illustrated an overview of the notions of form and meaning and the context model in SFL The combination of ... departure‖ namely Theme and the remainder of the clause, which develops Theme in order to complete the message of the clause, is known as Rheme As the starting point of the message‖, theme is commonly...

Ngày tải lên: 30/03/2015, 14:28

... validation of their structure and characterization of their functions Even after the identification, the next would be to understand how the molecular components of the cells are controlled, interact and ... third of the human genome consists of repetitive sequences Almost all of these have arisen by retroposition of an RNA intermediate followed by insertion of the resulting cDNA into the genome Of these, ... process of integration, the viral genome breaks at E1 and E2 regions, never at the E6 and E7 region The loss of 19 Literature Survey E2 results in the loss of E6 and E7 regulation This allows the...

Ngày tải lên: 16/09/2015, 17:13

... courts, and, on the other, secular jurisdictions, including the justice of the king, the justice of the lords, and the jurisdiction of municipalitks (cities), of corporations, and of trade.20 The ... that, with the concentration of the power of ennoblement in the hands of the king, statutory honor, founded on the recognition of peers and of others and affirmed and defended by challenge and prowess, ... forces (and of the direct claims of the dominantbbut strongly dependent upon the state The second factor is the degree of autonomy both of the educational system and of the scientific field from the...

Ngày tải lên: 16/02/2016, 09:14

... called Theme and Rheme The Theme served as the point of departure of the message (Halliday 1994:37), which in English coincides with the initial element(s) of the clause; and the Rheme is the remainder ... which is one of the typical features of a narrative 3.4.3 The Theme - Rheme Pattern The analysis shows that most of the themes in the text belong to topical theme Of 33 clauses and clause complexes ... of the text are taken into consideration 1.4 Methods of the Study The aim of this paper is to analyze the meanings and structures of a text The result should be useful for language teaching and...

Ngày tải lên: 07/09/2013, 13:48

Ngày tải lên: 12/12/2013, 00:15

... sources, the third states the phoric status of the tie, and the last presents the chain relation in terms of the address of all the preceding members in the chain Tables and summarise the total ... spaceship The planet They There The spaceship The two astronauts The woman It She We It Both of them They All plants and Animals They They Everything The man Nothing Something He I The engines He That ... complexes analysed for theme, 17 have Our analysis shows that most of the unmarked theme and have marked themes in the text belong to the plane of theme At the beginning of the text, ideational...

Ngày tải lên: 12/02/2014, 20:20

... cloned into pGEX-4T-2 The KIND domains of Spir-2 and PTPN13 were generated by RT-PCR and inserted into pEGFP-C1 and pGEX-4T-2 The N- and C-terminal regions of the v-KIND KIND2 and the MAP2 CD were ... proteins of these subregions (Fig S1C) and 1654 Fig Of all members of the KIND family of proteins, only KIND2 binds to MAP2 Pull-down assay (PD) of the endogenous MAP2 from cerebellar lysates of P21 ... downstream of v-KIND overexpression [12]) enhances the activity of MAP2 to bind to microtubules and promote their assembly [18], and that the inhibition of JNK1 (such as downstream of v-KIND knockdown...

Ngày tải lên: 14/02/2014, 19:20

... Mn-reconstituted enzyme and an additional water oxygen, which, together with the OD2 of Asp165, the NE2 of His79 and the NE2 of His169, formed an equatorial plane (Fig 3B) The metal ion and the addi˚ tional ... illustrates the superimposition of ApeSOD with the cambialistic SOD from P shermanii The overall structures of these enzymes were similar; the rmsd of the 747 Ca atoms was ˚ 0.695 A ApeSOD eluted from the ... ligand, NE2 of His31, bound to the metal, in the company of a water oxygen, from the apical positions The manga˚ nese was only 0.06 A out of the equatorial plane (Table 3) The angles around the...

Ngày tải lên: 14/02/2014, 22:20

Ngày tải lên: 15/02/2014, 17:20

... pocket structure As shown in Table 2, the hyperfine splitting constants of the 15N nucleus of the distal NO and of the 14N nucleus of the proximal His of the GmHO-1 complex are closer to those of the ... analyses of the molecular features of the heme–GmHO-1 complex and of the mechanism of heme degradation were performed, and the results were compared with those for the heme complexes of SynHO-1 and ... The spectrum of the extract is shown in Fig 8B, exhibiting peaks at 404, 534, 636 and 684 nm; the latter two differ from the 660 nm band of the protein complex (Fig 8A) When the solution of the...

Ngày tải lên: 19/02/2014, 05:20

... despite (i) the very similar structural architecture of OYE1 and PETN reductase, and (ii) geometry for the binding of steroid compounds and 2-cyclohexen-1-one [2,19] The structure of the Y186F ... resolved and are bound between the progesterone and the protein (Fig 7A) In comparison to the oxidized structures, the FMN isoalloxazine ring is less planar, with both the N5 atom and the C8-C7 ... s, and indicate re-oxidation of the flavin and the formation of the hydride-Meisenheimer complex of TNT (C) As for panel B except the spectral changes are recorded over an extended time period and...

Ngày tải lên: 20/02/2014, 02:21

... SDS/PAGE and in 2D PAGE (Figs and 2; Table 1) The N-terminal amino acid sequences (see above) allow the assignment of the HcA clone to the major sequence of the upper hemocyanin band in the SDS/PAGE ... regions and the entire 3¢ untranslated regions The standard polyadenylation signals (AATAAA) and the poly(A)-tails of different lengths are present in each clone The open reading frames of the five ... comparison of the patterns of the SDS, native and 2D electrophoresis, we assign HcA and HcB to the a and b polypeptides (that could not be separated in [14]), respectively, HcC to the c subunit, and...

Ngày tải lên: 20/02/2014, 11:20

... correlations The determination of the 3D structure of D desulfuricans ATCC 27774 NrfA [17] enabled to ascertain the spatial characterization of the five hemes, namely their proximity and the axial ... investigate if the spatial orientation of at least three of the four NrfH hemes could follow the heme arrangements observed in cytochrome c3 superfamily, and if the crystal structures of either cytochrome ... containing the double of NrfA subunit in respect to the NrfH subunit Nevertheless, inside these huge aggregates there are species of the type a2b2 The present findings are in agreement with the reported...

Ngày tải lên: 21/02/2014, 00:20