organizational factors the role of ethical culture and relationships

... position 13 of the other subunit [25,26], yielding a covalently bridged dimer The 4170 oxidized and reduced forms of the Y74C mutant had very different thermal stabilities While the stability of the ... and in the presence of denaturants [27] Extending these studies, we examine here the effect of increasing the bulk of the residue at position 74 Surprisingly, the Y74W mutant exhibited loss of ... across the dimer interface, while T75 interacts with N10 and E97 of the second subunit The backbone NH group of R98 forms a hydrogen bond with the backbone CO of F102 Furthermore, the orientation of...

Ngày tải lên: 18/02/2014, 11:20

Ngày tải lên: 18/02/2014, 11:20

... patterns of B2wt and of B1 ⁄ B2 chimeras reflect their agonist-inducible internalization The segment between the NPXXY motif and the conserved cysteine represents one of the regions with the highest ... influences the formation of a helical structure These authors proposed that activation of the receptor, and subsequently of the G protein, leads to a change in the environment of helix resulting in the ... to enhance their adherence Expression vectors The sequence of the human B2wt starting with the third encoded methionine [9], the sequence of the human B1wt, truncations and chimeras of both were...

Ngày tải lên: 07/03/2014, 16:20

... As the pKa values of the catalytic residues determine the pH optima, the ionization constants (pKa) of the catalytic glutamates (E187 and E399) in the ES complex and in the free enzyme (E) of ... the expression of the recombinant b-glycosidases Lysis of induced bacteria Fig Schematic representation of the Sfbgly50 active site E399 is the nucleophile and E187 is the proton donor Y331 and ... b-glucosidase affected the rate constant of the glycosylation step and also changed the enzyme pH optimum [14] Despite that, the effect of the tyrosine on the pKa values of the b-glycosidase catalytic glutamates...

Ngày tải lên: 23/03/2014, 15:21

... urea-induced and GdnHCl-induced changes in the secondary structure of the proteins, we analyzed the UV CD spectra of MS2 and VLPs in the absence and presence of 5.0 m and 9.0 m urea and 3.5 m and 5.0 ... determined the effects on stability of genetic fusion of the two subunits of the dlFG dimer using the variant named 2CTdlFG, which takes advantage of the physical proximity of the N-terminus and C-termius ... Effect of pressure on the coat protein in the presence of bis-ANS for (n) dlFG and (e) sc-dlFG and in the absence of the probe for (m) dlFG and (r) sc-dlFG, was analyzed at room temperature The...

Ngày tải lên: 30/03/2014, 11:20

... for explaining the determinants of economic growth The focus on labor and capital as the primary factors of production, and the general exclusion or trivialization of the role of knowledge, was ... only to the sphere of macroeconomics The most compelling theories of international trade were based on factors of capital and labor (and sometimes land) For example, the fundamental theorem for ... trade, the Heckscher-Ohlin theory, later extended to the Heckscher-Samuelson-Ohlin model focused on the factors of land, labor and capital According to the Heckscher-Ohlin theory, the proportion of...

Ngày tải lên: 01/06/2014, 11:20

... (ADC) on the the microcontroller, streamed to PC software Position sensors were attached to the thumb and forefinger, the wrist and the base of the object, to enable accurate Saunders and Vijayakumar ... random unpredictable delays to the hand controller In contrast to experiments and 2, where the control of the hand was repeatable and predictable, this experiment was designed to examine the role ... briefly before the start of the first trial, with full feedback sensibility, so that they could get used to the control mechanism of the hand Subjects performed the four blocks of the experiment...

Ngày tải lên: 19/06/2014, 08:20

... mineralizing RNA profile of igf-1, alp, and bmp-2 and significantly increased expression of other genes associated with mineralization like col1a1, fn, ilgf-1, noggin and oc Fgf-2, on the other hand, significantly ... matrix and promote healing [24] In this study, we have documented the relative efficiency of bone growth factors FGF-2, TGFb, and PGE2 markedly enhanced the synthesis of the total protein content of ... examined and photographed the cells on a Zeiss Axioskop using 20× Tritiated thymidine incorporation into DNA At the conclusion of the 24 hour incubation, the culture medium was removed and the cells...

Ngày tải lên: 20/06/2014, 04:20

... mineralizing RNA profile of igf-1, alp, and bmp-2 and significantly increased expression of other genes associated with mineralization like col1a1, fn, ilgf-1, noggin and oc Fgf-2, on the other hand, significantly ... matrix and promote healing [24] In this study, we have documented the relative efficiency of bone growth factors FGF-2, TGFb, and PGE2 markedly enhanced the synthesis of the total protein content of ... examined and photographed the cells on a Zeiss Axioskop using 20× Tritiated thymidine incorporation into DNA At the conclusion of the 24 hour incubation, the culture medium was removed and the cells...

Ngày tải lên: 20/06/2014, 07:20

Ngày tải lên: 20/06/2014, 21:20

Ngày tải lên: 21/06/2014, 17:20

Ngày tải lên: 21/06/2014, 19:20

... equality, the rule of law and respect for and protection of human rights […] Recognizing the need to strengthen the important role of the United Nations in the prevention of violent conflicts, and ... emerged from the growing realization of the need to put the poor and all aspects of their lives and means of living at the centre of development and humanitarian work, while maintaining the sustainability ... and development activities, and requests the Secretary-General to submit a detailed review of the capacity of the United Nations system in the context of the report on the implementation of the...

Ngày tải lên: 28/06/2014, 19:20

... increased the expression of the anti-apoptotic protein Bcl-xL, and increased the mobilization of p65 subunit molecules into the DC nucleus Furthermore, we found that the inhibition of NF-κB by ... (A) The optical density of each band was divided by that of the β-actin band, and the ratio at h was set at 100% (B) Fig NF-κB involvement in the taxol-induced effects on dendritic cells (DCs) The ... future study, the knockdown study of Bcl-xL using siRNA may further confirm their role of taxol-treated DCs Although the expression of Bax was increased in TaxolDCs, the expression of Bax occurred...

Ngày tải lên: 07/08/2014, 23:22

... Finally, the comparison between the different behaviour of the species tested suggests that resistance to the action of aerosols is the result of the strength of the structures protecting the leaf, and ... illustrate the results of a number of experiments the aim of which was to measure the toxic- ity of the various components of polluted sea spray, both in isolation and in synergy MATERIALS AND METHODS ... collected both near the coast and 2-3 km inland Samples of bulk deposits were taken from pine trees (Pinus pinea L) from the section of the crown exposed to the sea and also from the opposite side...

Ngày tải lên: 08/08/2014, 19:21

... as percentage of the production of untreated cells, and the standard error of the mean is given (n = 6–7) anisms behind the macrophage stimulation of other cells in OA cultures of synovial cells, ... contribution of these two proinflammatory cytokines on the production of each other, on other proinflammatory mediators, on the expression and production of various MMPs and TIMPs, and on ADAMTS4 and ... or the combination of the two (p < 0.01) (Figure 4a) We also studied the effect of Enbrel and/ or the neutralising anti-IL-1β antibody on the spontaneous production of the major MMPs (1, 3, 9, and...

Ngày tải lên: 09/08/2014, 08:23

... restores the susceptibility of skeletal muscles to IR injury irrespective of the other abnormalities in these mice The role of mast cells has not been considered when investigating the susceptibility ... the period of ischaemia, the muscle temperature during ischaemia and the period of reperfusion allowed for manifestation of the reperfusion injury Idstrom [12] utilised a period of 2, and h cold ... than the slow/fastand fast-twitch muscles Data from other studies are difficult to compare because of the wide variety of IR models in use In particular, there is a great deal of variation in the...

Ngày tải lên: 11/08/2014, 08:21

... above the umbilicus The SAAT was defined as the thickness between the skin-fat interface and the linea alba, and the VAT was defined as the distance between the anterior wall of the aorta and the ... obesity (BMI and WC, [21]) A second point of the problem to stress is whether the weight control can slow down the progression of IR and the worsening of fat deposition in organs in these obese ... MND, CD and PF helped to draft the manuscript TM and TG performed ultrasound TG participated in the design of the study and performed the statistical analysis All authors read and approved the final...

Ngày tải lên: 11/08/2014, 08:22

... measures have their advantages and disadvantages Serum levels of vitamins The assessment of serum levels of vitamins can have the advantage of being more objective than patient’s Page of reported ... questionnaires to assess the role of vitamins Other dietary factors -cured meats, fish, whole grains and alcohol- that have been reported to be associated with the risk of chronic obstructive pulmonary ... assessment of vitamins has the disadvantage that they represent the more recent intake, and for some vitamins such as vitamin C, the levels in peripheral blood are not representative of intake and not...

Ngày tải lên: 12/08/2014, 13:22