ora of 258 differentially expressed genes

... gills [3] Furthermore, several genes associated with the regulation of cell homeostasis were differentially expressed in our study Some genes were differentially expressed in both tissues, showing ... underexpressed at days 3, and 24 and day 7, respectively Discussion Of 322 genes identified in this study, 191 partial sequences had not been identified previously in C gigas Of the 131 known genes, ... between these two tissues A number of genes that were highly expressed in gills showed a biphasic expression pattern, consisting of a strong 6396 14 G Days of exposure Tissue Ras family GTP-binding...

Ngày tải lên: 18/02/2014, 16:20

... reproducible means to identify differentially expressed genes Effect of gene-specific knockdown on the accumulation of BaMV To investigate the roles of the differentially expressed genes identified by cDNA-AFLP ... down homologous genes in Page of 12 N benthamiana [29], was adopted to generate genespecific knockdown plants We evaluated the effects of lowered expression levels of individual host genes on the ... approximately three-fifths of the 15 randomly picked, differentially expressed genes showed either a positive or a negative influence on the accumulation of BaMV in plants Page of 12 Discussion The...

Ngày tải lên: 11/08/2014, 11:21

... tissues Out of the 2,067 total differentially expressed genes identified, 127 genes were found to be differentially expressed in both tissues, 115 of which were consistently differentially expressed, ... and 1,678 genes were down-regulated Since it is known that fewer genes are expressed in male gametes of plants [10], it is not surprising to see much few differentially expressed (DE) genes were ... genes in anthers and 1,864 genes in ovules were identified as being differentially expressed in the semigametic genotype 57-4 relative to Pima S-1 Although the list of common differentially expressed...

Ngày tải lên: 11/08/2014, 11:21

... expressed genes in order of significance by applying each procedure to a large number of subsets of arrays of a given size These genes were then compared with the 'master' list of differentially expressed ... list of 100 genes from the 2v2 GEO method contains just over 10 genes from the top 50 genes interactions To compare the effectiveness of the methods, we determined lists of differentially expressed ... number of patients are randomly selected from each group and a variety of methods were used to determine a list of differentially expressed genes We are mainly interested in very small sizes of one...

Ngày tải lên: 14/08/2014, 14:21

... for detecting differentially expressed genes using Affymetrix expression arrays BMC Genomics 2007, 8:96 Opgen-Rhein R, Strimmer K: Accurate ranking of differentially expressed genes by a distribution-free ... testing for differentially expressed genes in microarray experiments BMC Bioinformatics 2006, 7:538 Zhang : An improved nonparametric approach for detecting differentially expressed genes with ... actually be differentially expressed The analysis of many datasets is however practically difficult because of wide range of knowledge it would require, and this related to the next task Effect of different...

Ngày tải lên: 12/08/2014, 17:20

... Thus, cloning and characterizing genes that are differentially expressed between hybrids and their parents should provide further insights into the genetic basis of phenotypic differences In this ... activity of ACL is regulated by diet regimen and insulin [6] It is generally believed that changes in ACL activity in terms of the de novo lipogenesis state are due to alterations in the rate of its ... initial density of 60–80% and cultured overnight to ensure adhesion and spreading Co-transfections were then performed using lL of Lipofectamine 2000 reagent (Invitrogen) with lg of the firefly luciferase...

Ngày tải lên: 14/08/2014, 13:21

... Efron et al [5] used the 90th percentile of the standard errors of all the genes, i.e., for 90% of the genes, a is larger than the usual denominator of the t-statistic ( s2 /n1 + s2 /n2 ) Although, ... differentially expressed genes are shown in Table I The bootstrap-t-test yielded the lowest FDRe of 4%, i.e only out of the 200 genes are expected to be false discoveries, which shows that the power of the ... for 90% of the genes that show no treatment effect The treatment effect is for 10% of the genes The statistical significance of the treatment was tested by a t-test at a type-I-error rate of 0.01...

Ngày tải lên: 14/08/2014, 13:22

... software packages used by each team for the detection of differentially expressed genes 642 F Jaffrézic et al Detection of differentially expressed genes 643 challenging bacteria Differentially expressed ... Various comparisons of lists of differentially expressed genes found by the EADGENE teams were performed We focussed mainly on the comparison of the differentially expressed genes found between ... number of differentially expressed genes between these two time points, the lists of genes were still dependent on the method chosen Figure gives the Venn diagram for the differentially expressed genes...

Ngày tải lên: 14/08/2014, 13:22

... specificity of the comparison with the database Two conditions showed the same set of up regulated genes One condition found with both the randomly selected set of genes and the LPE specific set of genes ... a non-specific induction of a large spectrum of genes by an antifungal compound of unknown mechanism of action [9] The second condition corresponds to 17 out of the 22 genes that are induced by ... false discovery rate, we first counted the number of expressedgenes based on SAM for transformed data Then we selected the same number of expressedgenes from other tests based on their p-values...

Ngày tải lên: 14/08/2014, 14:21

... highly significant genes (p

Ngày tải lên: 14/08/2014, 14:21

... 0.6 0.8 Recall Use of differentially and constantly expressed genes to rediscover disease genes Figure Use of differentially and constantly expressed genes to rediscover disease genes The DER was ... diseaseassociated genes by combining GAD [25] and HGMD [26], resulting in a list of 3,221 genes with disease-associated variants We compared our list of differentially expressed genes with the list of genes ... count of GEO datasets in which a gene was differentially expressed divided by the count of GEO datasets in which it was measured For any cutoff x, differentially expressed genes were defined as genes...

Ngày tải lên: 14/08/2014, 21:20

... Table of contents Acknowledgment i Table of contents ii Summary vi List of Tables vii List of Figures viii List of Abbreviation ix Chapter Introduction 1.1 Dengue virus 1.1.1 Classification of dengue ... influence the level of enhancement in ADE infections 1.3.1 Specificity of antibody Most documented in vitro studies of ADE of DENV utilises antibody that target E protein of the viruses However, ... cycle in host cell 1.1.4 Pathogenesis of disease 1.1.5 Dengue Epidemiology 11 1.1.6 Treatment of dengue 13 1.2 Antibody Dependent Enhancement (ADE) 14 1.2.1 Mechanisms of ADE 15 1.2.2 Fc-FcR mediated...

Ngày tải lên: 13/10/2015, 16:41

... levels of expression of the AtPasp A1, A2 and A3 genes using Northern blot hybridization of total RNA from different tissues These results confirmed that the three genes are differentially expressed ... specific probes The AtPasp A1 and A3 genes are both expressed in flowers, while all three genes are expressed in seed pods In seeds, we found expression of all three genes in all seed cell types (Fig ... the expression of the two seed storage proteins of Arabidopsis, 12S globulin and 2S albumin [42,43] The expression of our proteinase genes in the same cell types as the seed storage proteins...

Ngày tải lên: 17/03/2014, 11:20

... the putative function of the 32 genes Page of 10 tested by qPCR and their pattern of expression, we suggested in the figure a possible representation of involvement of these genes dureint the interaction ... of 10 Additional file 1: Bioinformatic annotation of cDNA-AFLP ESTs identified as differentially regulated in the Malus - E amylovora host-pathogen interaction list of clones differentially expressed ... three pathogenesis-related (PR) genes of apple, PR-2, PR-5 and PR-8, are also induced in response to inoculation with E amylovora [18] Additionally, infection of apple by E amylovora results...

Ngày tải lên: 12/08/2014, 03:21

... because of the higher concentrations of pesticides (between gÆL)1 and mgÆL)1, according to the herbicide) and shorter time of exposure (between and 24 h) used by the authors In another study, 258 differentially ... regulation of specific herbicideregulated gene expression Results Identification of herbicide-regulated genes Two forward and reverse SSH libraries were made from pooled digestive glands and gills of C ... herbicide-regulated genes The time-dependent expression of 13 genes that were up-regulated by exposure to herbicide and five that 391 Oyster response to pesticide exposure A Tanguy et al Table Up-regulated genes...

Ngày tải lên: 30/03/2014, 15:20

... Figure alignment of deduced amino acid sequences of HdIV and selected ichnoviruses rep genes ClutalX alignment of deduced amino acid sequences of HdIV and selected ichnoviruses rep genes The first ... rep12 TD Figure genes identified in and by relative quantitative PCR Expression profilesHdIV gene copy number of the 10 rep Expression profiles and gene copy number of the 10 rep genes identified ... volume of saline buffer (PBS) Southern blot analysis for identification of HdIV rep genes Identification of HdIV segments containing the new rep genes was carried out by Southern blot analysis μg of...

Ngày tải lên: 20/06/2014, 01:20

... 12,000 genes, and although there is a significant decrease in the numbers of Table The number of groups of genes, and total numbers of genes in groups, that are identified at various levels of significance ... pattern of gene expression that mirrors this Journal of Biology 2002, 1:5 http://jbiol.com/content/1/1/5 Journal of Biology 2002, Table The number of groups of genes, and total numbers of genes ... much larger regions of the human genome show a strong clustering of highly expressed genes [6], which is caused by http://jbiol.com/content/1/1/5 clustering of genes that are expressed in nearly...

Ngày tải lên: 06/08/2014, 18:20

... sensitivity of guard cells to ABA [10] We used the atmyb60-1 allele to investigate the role of VvMYB60 in the regulation of stomatal activity and to Page of 14 explore the conservation of the MYB60 ... speculate that Page of 14 the presence of the former and the absence of the latter, might contribute to the specificity of the MYB60 function in guard cells Expression features of VvMYB60 in grape ... the onset of ripening and the accumulation of sugars are correlated to increasing levels of ABA in the berry [30], suggesting a possible negative effect of the hormone on the expression of VvMYB60...

Ngày tải lên: 11/08/2014, 11:21

... Profiling of genes that are expressed in sensory neurons might therefore provide insights into the genes required for the specific functions of neurons To identify sensory neuron -expressed genes, ... enrichment of ciliated neuronexpressed genes Expression analysis of candidate sensory neuronexpressed genes by reporter fusions The above analyses showed that sensory neuron -expressed genes were ... the volume of target neurons was much smaller than that of muscles, transcripts of various sensory neuron -expressed genes, ranging from those expressed in many sensory neurons to those expressed...

Ngày tải lên: 14/08/2014, 14:21

... hybridized provided that the majority of genes are not differentially expressed [5] What is not clear is how large the proportion of differentially expressed genes can be before lowess becomes unsuitable ... differentially expressed genes when the default number of iterations is equal to (b) For 10 robustifying iterations the normalization is reliable for approximately 25% of genes differentially expressed ... boutique array designed specifically to profile differentially expressed genes during the late stages of B-lymphocyte differentiation On these arrays 109 genes of interest have each been spotted four...

Ngày tải lên: 14/08/2014, 17:22