or tasted by real testers

... first step for the clarification of microcystin degradation Acknowledgements This work was supported by the Grant-in-Aid for Scientific Research Category "B" from the Japan Society for the Promotion ... cycles of denaturation at 95°C for 10 s Annealing was then performed for QMF, QMR, and QMT at 65°C for 35 s; and for BACT1369F, PROK1492R, and TM1389BACT2 at 56°C for 35 s The amplification reactions ... QMR by Applied Biosystems Co USA Thermal cycling was carried out as follows: initial denaturation at 95°C for min, followed by 35 cycles of denaturation at 95 °C for 10 s, annealing at 55°C for...

Ngày tải lên: 05/09/2013, 10:15

... by averaging a moving window of 300 points ($ h of data) This  smoothed signal, which we denote by P40 , was used to normalize the other signals from the mass analyzer in order to correct for ... possible factors First, one particular oscillator whose existence was revealed by this experiment could be chaotic of itself The second and perhaps more likely possibility is that these oscillators (and ... time and has a more complex waveform in the latter Fourier analysis of windows of the data set where this oscillatory mode is particularly easily resolved in the normalized O2 record from the mass...

Ngày tải lên: 07/03/2014, 10:20

... employees could work better together as a team You know they could support each other more - and help each other out more when needed You know they could be more unified as a team, believe more in the ... passionate, engaged and deliver their best work - the reality is this is seldom the case More likely, the engagement of your workforce follows the statistics suggested by the research company, Gartner Group ... typically only 20 to 25% of the workforce is fully engaged, motivated, passionate and working for the good of the company Gartner suggests that as many as 20% of a workforce are unmotivated, disengaged...

Ngày tải lên: 08/03/2014, 15:20

... "The more years of education, the more you are likely to know" before immediately changing opinion Informing us that "more education was not generally related to a better understanding of more ... simpler task for them to achieve their targets with honest and realistic answers For example, looking at a project carried out by The Glasgow Media Group regarding the 1980's minors strike, one ... questions or points, which could be raised My main aim would be for the supposed 'questions' to be more like open discussion points So, for example, one could be presented with a sentence or paragraph...

Ngày tải lên: 21/03/2014, 22:06

... either stored at different temperatures (see below) or treated immediately for RNA extraction Sterile hoods, incubators or ≤-20°C refrigerators were not required RNA processing and Real Time ... Little Chalfont, UK) for 18 h and then harvested Tracer incorporation was measured by β-counting Phenotypic characterization of cells PBMC were phenotyped by staining with FITC- or PE-conjugated mouse ... incubated at 37°C for hour Two μl of cDNA were used for each PCR amplification by "real time" technology (7300 Real Time PCR system, Applied Biosystems, Rotkreuz, Switzerland) according to manufacturer's...

Ngày tải lên: 18/06/2014, 15:20

... Braintree, UK) or Rotorgene 3000 (Corbett Research, Sydney, Australia) thermal cycler: 95°C for 15 minutes, followed by 40 cycles of 94°C for 15s, 68°C for 30s and 76°C for 30s Fluorescence was ... author(s) declare that they have no competing interests Authors' contributions The study was designed by MJH and EANA; experimental work was done by EANA, MJH and AN; interpretation and laboratory ... temperatures were 95°C for 15 minutes, followed by 40 cycles of 94°C for 15s, 58°C for 30s and 72°C for 30s At the end of amplification PCR products were subjected to a dissociation or melting curve...

Ngày tải lên: 19/06/2014, 08:20

... Braintree, UK) or Rotorgene 3000 (Corbett Research, Sydney, Australia) thermal cycler: 95°C for 15 minutes, followed by 40 cycles of 94°C for 15s, 68°C for 30s and 76°C for 30s Fluorescence was ... author(s) declare that they have no competing interests Authors' contributions The study was designed by MJH and EANA; experimental work was done by EANA, MJH and AN; interpretation and laboratory ... temperatures were 95°C for 15 minutes, followed by 40 cycles of 94°C for 15s, 58°C for 30s and 72°C for 30s At the end of amplification PCR products were subjected to a dissociation or melting curve...

Ngày tải lên: 20/06/2014, 04:20

... less anxious and depressed, report more vitality and have a better mental functioning [22,25,26] Moreover, patients with a higher score on indicators of CAT reported better physical functioning ... resources By studying adaptation Taylor [19] developed the Cognitive Adaptation Theory (CAT) which is based on cognitive interviews with chronically ill persons This theory is one of the dominant theories ... is available for investigating the ability to adapt based on CAT Studies have used different indicators and methods for their analyses For instance, studies have included indicators measuring...

Ngày tải lên: 20/06/2014, 15:20

... Kit following by UltraClean Soil DNA Kit (MoBio Laboratories) The average DNA quantity extracted was 3.51 ± 1.53 ng/μl, and DNA quality was evaluated by gel electrophoresis before the chain reaction ... Table The results for these groups were expressed as cycle threshold (Ct) or as 1/Ct, where relative abundance was discussed for each reaction, instead of real quantification, as for the Methanosarcinaeae ... 6% for msar amplification and below 15% for msa, mrtA and mcp amplifications Statistics Statistical analyses were performed using the SPSS Package, version 17.0, for Windows A Spearman correlation...

Ngày tải lên: 20/06/2014, 23:20

... sesuac htworg laiborcim lamroN ]9[ scitoibitna dna stnemelppus ,eulav Hp ot tcepser htiw reffid sebut ehT rotacidni Hp eht sa elprup losercomorb htiw raga ni nworg era serops sulihpomrehtoraets ... sulihpomrehtoraets B elprup niamer ot rotacidni Hp eht fo roloc eht esuac htworg laiborcim lamron tibihni taht secnatsbuS noitulos ni wolley 971 yrtluop ni eudiser laidiccocitna rof neercs ot elprup morf ... ni lortnoc evitisop a sa desu saw gurd hcae fo noitulos lm/gµ 05 a dna lortnoc evitagen eht sa desu saw retaw dellitsiD selpmas lortnoC la te idnatihS olakanA 871 eht ni öjsenretS esÅ rosseforP...

Ngày tải lên: 07/08/2014, 18:21

... and 40 cycles of 95°C for 15 s/60°C for The fluorescent signal at each cycle generated by the release of fluorophores (FAM or JOE) from the quencher (TAMRA) by the 5′-exonuclease activity of AmpliTaq ... generated by real- time PCR using cDNA from stimulated PBMC daily for five consecutive days A known aliquot of PBMC was assayed for GAPDH on each day and either the raw C(t) was recorded or the amount ... Table for the CV values) To correct for this influence, the CE values of the target genes are normalized to a reference gene using the CE for GAPDH The RE compared with GAPDH is determined by dividing...

Ngày tải lên: 09/08/2014, 01:23

... determined according to the guidelines of the Clinical and Laboratory Standards Institute (CLSI) for in vitro antifungal susceptibility testing [12] The RPMI medium 1640 (31800-022) provided by Gibco ... test or Fisher's exact test were used for categorical variables The Student T-test was used for continuous variables Logistic regression was used to assess the independent effect of factors that ... resistance The mechanism by which the usage of antituberculous agents is a risk for colonizing or infecting with fluconazole resistant strains is worthy of further study by a larger series Abbreviations...

Ngày tải lên: 11/08/2014, 07:21

... S/D Oral biopsy N/D - - A2 S/D Oral biopsy N/D - - A3 S/D Oral biopsy N/D - - A4 S/D Oral biopsy N/D - - A5 S/D Oral biopsy N/D - - A6 S/D Oral biopsy N/D - - A7 S/D Oral biopsy N/D - - A8 S/D Oral ... N/D - - A9 S/D Oral biopsy N/D - - A10 S/D Oral biopsy N/D - - A11 S/D Oral biopsy N/D - - A12 S/D Oral biopsy N/D - - B1 S/D Oral biopsy N/D - - B2 S/D Oral biopsy N/D - - B3 S/D Oral biopsy N/D ... N/D - - B4 S/D Oral biopsy N/D - - B5 S/D Oral biopsy N/D - - B6 S/D Oral biopsy N/D - - B7 S/D Oral biopsy N/D - - B8 S/D Oral biopsy N/D - - B9 S/D Oral biopsy N/D - - B10 S/D Oral biopsy N/D...

Ngày tải lên: 12/08/2014, 04:20

... supported by FORMAS, SLF, EU (FOOD-CT-2004-513928), by Grant No 143286 from the Norwegian Research Council and by MERIAL The authors would also like to thank Anja Bråthen Kristoffersen for assistance ... those recorded for the Norwegian pigs, the cortisol levels for the three herds were all within the normal range [32] and no extreme stress-related behaviour such as tail-biting were recorded in ... those recorded for the Norwegian pigs, the cortisol levels for the three herds were all within the normal range [32] and no extreme stress-related behaviour such as tail-biting were recorded in...

Ngày tải lên: 12/08/2014, 18:22

... 14:437 http://ccforum.com/content/14/4/437 Author details Department of Intensive Care Medicine, Academic Medical Center, Meibergdreef 9, 1105 AZ Amsterdam, The Netherlands 2Laboratory of Experimental ... natural surfactant for treatment of acute lung injury and the acute respiratory distress syndrome Am J Respir Crit Care Med 2009,180:989-994 Oczenski W, Hormann C, Keller C, Lorenzl N, Kepka A, ... Findlay GP, Rouby JJ, Holzapfel L, Bruins P, doi:10.1186/cc9211 Cite this article as: Schultz MJ.: Lung reaeration after surfactant instillation caused by surfactant or caused by instillation?...

Ngày tải lên: 13/08/2014, 21:21

... blood for DNA Detection Kit was stored at -20°C for up to 72 hours before testing The storage did not affect the assay performance The detection sensitivity of SeptiFast is 30 colony-forming ... condition that fulfilled two or more of the following criteria [7]: temperature > 38°C or < 36°C; heart rate > 90 beats per minute; respiratory rate > 20 breaths per minute or PaCO2 < 32 mmHg; white ... for SeptiFast analysis Ten milliliters of whole blood were collected for DNA Detection Kit analysis immediately after blood collection for microbial culture 1.5 mL were used for the assay for...

Ngày tải lên: 13/08/2014, 21:21

... a gravity feeder or a force feeder The orientation of the feeder depends on the roller compactor design and it could be vertical, horizontal or inclined Feeder designs are important towards ensuring ... for UV or colorimetric determinations Moreover, the NIR spectra are also affected by the physical properties and measuring conditions which make the analytical method even more complex Therefore, ... undergo densification, deformation or fragmentation and bonding under pressure to form flakes Higher compaction force leads to the formation of stronger flakes with a lower porosity and less fines...

Ngày tải lên: 09/09/2015, 18:58

... Absorbance readings for thyroid RNA in Balb/c mice 39 Table Absorbance readings for orbit RNA in Balb/c mice 40 Table Absorbance readings for spleen RNA in Swiss Outbred mice 41 Table Absorbance ... illuminated by a laser Each cell is measured for size (forward light scatter) and granularity (90o light scatter), as well as for presence of colored fluorescence Thus by measuring the fluorescence ... network of genetic factors 17 governs the response of the immune system Genetic factors such as HLA, T cell regulatory gene, polymorphisms in cytokine, cytokine receptors, and tolllike receptors...

Ngày tải lên: 07/10/2015, 09:55

... Therefore, rows 1, 2, of W are linearly independent Therefore, W has rank 3, that is, = Note that post-multiplying W by an invertible (real) matrix, or pre-multiplying W by an invertible (real) ... denoting by mr(SG ) is commonly denoted by mr(G) in papers that study only the minimum rank of the real symmetric matrices described by a graph, and F mr(SG ) is sometimes denoted by mr(F, G) or mrF ... Therefore, a = −1±2 −3 , which contradicts the fact that W is a real matrix Therefore, there is no real matrix whose cycle matrix is CS1 Corollary 2.4 mr(MRS ) = > = mr(MCS ) C C 1 Proof By Lemma...

Ngày tải lên: 07/08/2014, 15:23

... variability of animal infectivity assay, or other factors - 43 - Journal of Water and Environment Technology, Vol.3, No.1, 2005 ACKNOWLEDGMENT This research was supported by funds from the National Science ... D 1989 Inactivation of Health-related Microorganisms in Water by Disinfection Processes Water Science and Technology 21: 179-195 Sykora J L., C A Sorber, W Jakubowski, L W Casson, P D Gavaghan, ... inactivation of G lamblia cysts by LP UV in both media were relatively similar to each other Meanwhile, the inactivation of G lamblia cysts by MP UV in the filtered water was even more rapid and reached...

Ngày tải lên: 05/09/2013, 09:08