operational strategies of plant cell and tissue culture bioreactors

... calculation cell densities of Cell culture §ubculture of suspension cells Subculture of adherent cells Rejection of failed cell cultures Cryoprese~ation i arations of sterile filtered D Preparation of ... line WI-38 Cell line Namalwa Cell line BHK-21 Cell line MDCK Cell line GH3 Cell line 293 Cell line !VCRE/!VCRIP References 1.3 Master and Working Cell Banks Scale and composition of cell banks ... 4.3 xvi CULTURE CELL AND TISSUE Angela Martin National Cell and Tissue Culture Centre, Bioscience Ireland, School of Biological Sciences, Dublin City University, Glasnevin, Dublin 9, Ireland Section:...

Ngày tải lên: 10/04/2014, 11:12

... "primary culture" and "culture of established cell lines." Primary culture can consist of the culture of a complex organ or tissue slice, a defined mixture of cells, or highly purified cells isolated ... INTRODUCTION TO CELL AND TISSUE CULTURE: Theory and Technique Jennie P Mather and Penelope E Roberts Page iii Introduction to Cell and Tissue Culture Theory and Technique Jennie P Mather and Penelope ... mammalian cell in vitro The Practice and Theory of Tissue Culture This book is meant to serve as an introduction to cell culture both for students who have little or no experience of cell culture and...

Ngày tải lên: 11/04/2014, 09:48

... formation of new shoots) and were transplanted in the nursery (NAA) + mixture Propagation by tissue culture Plant material For initiation of Q robur and Q petraea organ were taken from shoots of seedlings ... and conditions Organ cultures Explants were cultured on modified GresshoffDoy (GD) medium (Gresshoff and Doy, 1972), BTM (Chalupa, 1984), or Woody plant medium (WPM) (Lloyd and McCown, 1980) The ... embryogenesis Explants were cultured on modified MurashigeSkoog (MS) medium (Murashige and Skoog, 1962), Schenk-Hildebrandt (SH) medium (Schenk and Hildebrandt, 1972), and WPM (Lloyd and McCown,...

Ngày tải lên: 08/08/2014, 19:21

... accuracy of automated measurements of transduction efficiency Cell types were determined by examining nuclear and cellular morphology Results Developmental expression of a- and b-DG in liver tissue ... investigate the expression of a- and b-DG in the liver at pre- and postnatal time points and document the in vivo tissue tropisms of these LCMV pseudotypes in neonatal and adult mice We hypothesized ... isolates WE54, LCMV Cl13, and Traub efficiently target antigen presenting cells (dendritic cells) in the spleen and perturb their ability to present antigen to T cells and B cells, resulting in a...

Ngày tải lên: 14/08/2014, 19:22

... establishment of cell suspension cultures; (5) analysis of metabolite levels in cell suspension cultures; (6) selection of cell lines based on single cells; (7) analysis of culture stability; and (8) ... functional status of cells is now routinely performed for plant cell cultures in order to permit accurate assessment of growth and metabolite production rates The availability of plant cells for quantitative ... application of plant metabolic engineering that is based on a thorough knowledge of plant biochemistry and plant physiology Plant metabolism itself concerns thousands of interacting pathways and processes...

Ngày tải lên: 25/10/2013, 05:20

... Durand & AquinoParsons that blood flow decreases could transiently arrest the division of tumour cells in S-phase [62] These cells are the main targets of chemotherapy, and the arrest of their cell ... hypoxia on cancer cells and endothelial cells within a tumour Fig Schematic representation of the effects of HIF-1 activation under intermittent hypoxia on cancer cells and endothelial cells within ... targets of these treatments comprises the endothelial cells One endothelial cell can control the survival of approximately 50–100 tumour cells [93] Therefore, the destruction of a few endothelial cells...

Ngày tải lên: 30/03/2014, 04:20

... in the range of to million per gram of lung tissue The CD11c+ cells were then seeded into 12-well plates at 105 cells per well and cultured for 48 hours in the presence of 10 mg of chitosanpVAX ... U/mL) in the presence of DNase I (200 mg/mL) and passed through a 40-micron cell strainer to prepare single -cell suspensions This is a standard method of cell preparation and does not result in ... maceration of the lymph nodes through 40-micron cell strainers, and CD11c+ cells were isolated by magnetic bead separation The cells were stained for CD11b and separately for each of the dendritic cell...

Ngày tải lên: 08/08/2014, 21:20

... best of a subset of predictors randomly chosen at that node Onethird of the cases are left out of each bootstrap sample and not used in the construction of any given decision tree Each of these ... aspirations ANA, JML, CG and CB participated in the design of the study and interpretation of data MS participated in the design of the study and interpretation of data, and was involved in drafting ... by significantly elevated levels of T cell related cytokines (IL-2, IL-4, IL-13 and IL-17) and stromal cell and macrophage related cytokines (EGF, bFGF, IL-1 and IL-15) when compared with synovial...

Ngày tải lên: 09/08/2014, 06:22

... line is this: combining stem cell and genome technologies is a terrific idea I foresee a deeper understanding of human development and disease as a result of this union and, thus, a shorter path ... expectations for the fruits of stem cell and personal genomics research are high but the general understanding of each, particularly their limitations, is low This gap in public understanding is a particular ... C: Do Scientists Understand the Public? Cambridge, Massachusetts: American Academy of Arts and Sciences; 2010 11 Murdoch CE, Scott CT: Stem cell tourism and the power of hope Am J Bioeth 2010,...

Ngày tải lên: 11/08/2014, 12:21

... concentrations of purified 17-kD endo-1,3-β-glucanase and 40- and 45-kD cellulases were able to inhibit the germination of encysted zoospores and elongation of germ tubes of a plant- pathogenic ... hyphal cell walls of F oxysporum f.sp lycopersici race 2, containing glucan as the principal component of its cell walls The lack of cellulase and xylanase activities (acting on plant cell wall ... endoβ-1-3-glucanase, and N-acetylglucosaminidase simultaneously in the roots of soybean seedlings and in cell- free culture filtrates of T harzianum isolate Th008 With the exception of that of endochitinase,...

Ngày tải lên: 11/08/2014, 15:20

... expression and subsequent siRNA accumulation in tobacco plants as a function of time GFP expression and subsequent siRNA accumulation in tobacco plants as a function of time Leaves of tobacco plants ... replication seem to be a general feature of most of the plants The intensities of the 1.4 kb bands, as shown in Fig 2A-D, were quantified using ImageQuant TL software and were normalized with respect ... removal of these proteins might negatively impact VA accumulation and hence investigated the effect of null mutation of AC2 and AC4, both singly and in combination, on the replication efficiencies of...

Ngày tải lên: 12/08/2014, 04:20

... subunit of the human holoenzyme telomerase RT and T antigen of simian virus 40 in a primary culture of VSMCs as previously described [19] Immortalised VSMCs were cultured in Smooth Muscle Cell Basal ... pathophysiology of GCA and the ongoing inflammatory process in the arterial wall The combined use of 2-DE and immunoblotting offers an interesting approach to the identification of target antigens of autoantibodies ... the proteome of VSMCs and HUVECs are present in the sera of patients with GCA These antibodies recognise cellular targets that play key roles in cell biology and the maintenance of homeostasis...

Ngày tải lên: 12/08/2014, 17:22

... of coagulation and of the protein C system and the release of other markers of inflammation and endothelial activation early after trauma Here, we report an extracellular release of HMGB1 within ... severity of injury, tissue hypoperfusion, activation of the protein C system and coagulation abnormalities, complement activation and the release of other biomarkers of endothelial cell activation ... part of the standard management of major trauma patients The base deficit was used as a measure of the degree of tissue hypoperfusion Admission base deficit is a clinically useful early marker of...

Ngày tải lên: 13/08/2014, 19:20

... system); ozone (plants submitted to 200 nl l-1 of O3 for and 20 h); cold and freezing (leaves harvested from plants acclimated at days of +2°C and from plants exposed to two and seven days of -2°C following ... a -30 b b c Metabolic profiling -35 A B C P Figure Estimation of salt and drought stress in area A, B, C, and P Estimation of salt and drought stress in area A, B, C, and P Ion concentrations ... response to extracellular stimulus GO:0009991 11 GO:0007165 150 signal transduction cell death GO:0008219 33 cell differentiation GO:0030154 reviews cell- cell signaling 87 cell growth and/ or maintenance...

Ngày tải lên: 14/08/2014, 15:20

... transporters .47 Aims and overview of study 50 Materials and Methods 52 3.1 Mammalian cell culture 52 3.1.1 Materials 52 3.1.2 Cell line and cell culture ... grateful to the members of my family for their understanding and great support, especially to my dear parents, sister and husband, for their loving encouragement and caring Table of Contents Acknowledgements ... Hep G2 cells 99 Figure 4.12 Immunostaining of Hep G2 cells overexpressing wild-type and mutant MRP4 101 Figure 4.13 Efflux of bimane-GS from mutant MRP4/Hep G2 cells and controls...

Ngày tải lên: 05/10/2015, 13:53

... China Hainan, Republic of Vietnam In Vietnam, Laos, Cambodia and tropical regions of Myanmar, Thailand In Vietnam, the tropical regions of Southwest and South of China Taiwan and Philippines Himalaya ... Cambodia, Thailand and Myanmar In Indochina and India In Vietnam, Malaysian peninsula and islands of Malaysia In Vietnam, Malaysian and Indonesia In Vietnam, Malaysian, Indonesia and Australia ... Philippines and islands on Pacific In the tropical regions of Asia, Africa and Australia In the tropical regions of America, Asia, Africa In North Korea, Japan, South of China, Taiwan and North of Vietnam...

Ngày tải lên: 11/07/2016, 17:20

... established cell suspension culture of ginseng cell and some attempts have been made to increase biomass and ginsenoside yield of ginseng cell culture Materials and methods Stock cell culture and culture ... [5] Hibino K and K Ushiyama 1999 Commercial production of ginseng by plant tissue culture technology In: T.J Fun, G Singh, W.R Curtis [13] [14] [15] 195 (Eds.), Plant Cell and Tissue Culture for ... Institute Inc., Cary, USA) Results and discussion To understand the culture characteristics of the suspended cells of ginseng in shake flask, the effect of plant growth regulators (2,4-D or IBA...

Ngày tải lên: 14/03/2014, 10:20

... any SOFC system, the performance of the INER SOFC system is dependent not only on the design and operating conditions of the fuel cell stack, but also on the design and operating conditions of ... provided by the anode off-gas given an anode off-gas temperature of less than 650 oC, a cathode off-gas temperature of less than 390 oC, and a flame barrier temperature of less than 700 oC However, ... an oxidant-to-carbon ratio (O/C) of 0.3 Following completion of the optimization process shown in Figure (yielding a cell voltage and current density of 0.78 V and 400 A/cm2, respectively), the...

Ngày tải lên: 05/09/2013, 16:10

... presence of Kdn might be characteristic of plant pathogenic streptomycete strains causing scab diseases of potatoes and root crops Further studies of the cell wall anionic polymers in the plant ... Hz) and 5.07 (J1,2 3.6 Hz) and two signals of lower intensities at d4.55 (J1,2 7.9 Hz) and 4.66 (J1,2 7.9 Hz) (Table 2) Two signals at d1.93 and 2.07 were observed in the region of resonances of ... 62.0, 65.8, 68,0, and 69.4 Other signals of the spectrum were found at d67.9–80.4, i.e in the region of resonances of CH groups bound to one oxygen atom The region of resonances of the anomeric...

Ngày tải lên: 17/03/2014, 10:20

... multitude of SDj of this fam- comparing repertoire restriction of tissue with blood ily CDk of a sample was defined as sum of the moduli of Per-family normalized relative concentration P'jk of a fam- ... average given in fold of standard deviation of the respective family (C) The sum of the modulus' normalized concentrations of all families of the samples (CD) and the number of families elevated ... repertoire in tissue samples of CRC patients Twenty-four samples of normal colon tissue and 18 carcinoma samples were evaluable The statistical analysis of both CD and n(F) comparing all tissue samples...

Ngày tải lên: 18/06/2014, 16:20