of human peripheral blood mononuclear cells

... E, Comandini A, Forte G, Macchi B, Bonmassar E: Telomerase activity of human peripheral blood mononuclear cells in the course of HTLV type infection in vitro AIDS Res Hum Retroviruses 2002, 18:249-251 ... Tax-expressing HTLV-1-positive cells (MT-2, SLB-1, HT-1RV) expressed moderate levels of PTH1R RV-ATL cells expressed low levels of PTH1R while MET-1 cells did not express the PTH1R Human β2 microglobulin ... conjunction with other oncogenes The goal of this study was to investigate the expression of PTHrP, its receptor, and MIP-1α during the early stages of immortalization of human lymphocytes by HTLV-1 Using...

Ngày tải lên: 13/08/2014, 05:20

... Effects of SEB on human PBMCs C Mendis et al of the high activation of T cells and used it as our second parameter to evaluate the effectiveness of MK591 Our results clearly indicated the ability of ... 100 ND 38.5 ± 2.5 3091 Effects of SEB on human PBMCs C Mendis et al Effect of 5-LO inhibitor MK591 on monkeys challenged with SEB Findings of in vitro studies of human PBMCs were verified in PBMCs ... metabolites in human T cells and human lymphocytes, respectively [6] Products of the arachidonic acid metabolizing enzyme 5-LO have been shown to stimulate the growth of several types of cancers,...

Ngày tải lên: 30/03/2014, 04:20

... SEC1-treated human PBMC in rabbits is associated with the levels of IL-1b, IL-6 and ROS in the supernatant fluids of SEC1-treated human PBMC Adding Fig Membrane translocation of p47phox in SEC1-treated cells ... enterotoxin A acts through nitric oxide synthase mechanisms in human peripheral blood mononuclear cells to stimulate synthesis of pyrogenic cytokines Infect Immun 68, 2003–2008 60 Snitcowsky ... not only profoundly decreased the level of p47phox in the membrane, but also increased the level of p47phox in the cytoplasm The use of apocynin alone did not affect the translocation of p47phox,...

Ngày tải lên: 19/02/2014, 00:20

... donor peripheral mononuclear cells [16] As an extension of these studies, we now report the development of a novel in vitro method to induce human MDSC from healthy donor peripheral blood mononuclear ... characterization of human Cd33+ And Cd11b+ myeloid-derived suppressor cell subsets induced from peripheral blood mononuclear cells co-cultured with a diverse set of human tumor cell lines Journal of Translational ... Characterization of cytokine-induced myeloid-derived suppressor cells from normal human peripheral blood mononuclear cells J Immunol 2010, 185:2273-2284 Kusmartsev S, Gabrilovich DI: Effect of tumor-derived...

Ngày tải lên: 18/06/2014, 19:20

... tumor-elaborated substances are capable of reaching the immune system and peripheral blood stream Therefore, we hypothesized that the percent of peripheral blood mononuclear cells (PBMCs) displaying p-STAT-3 ... insufficient collection of intraoperative blood sometimes precluded this analysis Determination of Tregs in peripheral blood of patients withgliomas For subset analysis, after we became proficient at analyzing ... in immune cells in the cervical lymph nodes, which then are detected in the peripheral circulation Therefore, we measured p-STAT-3 in glioma patients' peripheral blood mononuclear cells (PBMCs)...

Ngày tải lên: 18/06/2014, 15:20

... transduce human peripheral blood mononuclear cells that were stimulated to proliferate with anti-CD3 and anti-CD28 Typically, only ~10% of cells (not shown) became infected as judged by expression of ... Figure CEA-specific proliferation of human lymphocytes measured by uptake of [3H]-thymidine CEA-specific proliferation of human lymphocytes measured by uptake of [3H]-thymidine T-bodies (labeled ... CEA-expressing cells CEA specific T bodies had startling therapeutic effects in a mouse model of human colon cancer Thus, this vector has potential for redirection of human PBLs to chimeric anti-tumor T cells, ...

Ngày tải lên: 19/06/2014, 08:20

... transduce human peripheral blood mononuclear cells that were stimulated to proliferate with anti-CD3 and anti-CD28 Typically, only ~10% of cells (not shown) became infected as judged by expression of ... Figure CEA-specific proliferation of human lymphocytes measured by uptake of [3H]-thymidine CEA-specific proliferation of human lymphocytes measured by uptake of [3H]-thymidine T-bodies (labeled ... CEA-expressing cells CEA specific T bodies had startling therapeutic effects in a mouse model of human colon cancer Thus, this vector has potential for redirection of human PBLs to chimeric anti-tumor T cells, ...

Ngày tải lên: 20/06/2014, 04:20

... tissues, profiling peripheral blood cells has emerged as an attractive biomarker discovery strategy [10,16-22] Another added advantage to analyzing peripheral blood cells is the fact that blood is ... present study, we profiled gene expression in rat peripheral blood mononuclear cells (PBMCs) during the development of CIA We established the method for blood collection, Page of 14 (page number ... expression profiling in peripheral blood mononuclear cells in the collagen-induced arthritis model To identify putative biomarkers for arthritis, we surveyed global gene expression profiles of PBMCs...

Ngày tải lên: 09/08/2014, 07:20

... inhibition show the importance of cytokines in RA [16] Induction of proinflammatory cytokines via cross-linking of FcγR by ICs may be a possible mechanism of activation of cells in the rheumatic joint ... and the University Hospital in Uppsala Preparation of peripheral blood mononuclear cells and cell cultures Buffy coats obtained from healthy blood donors were diluted in PBS at room temperature ... regulate cytokine production by peripheral blood mononuclear cells Clin Immunol 1999, 93:274-282 33 Nilsson UR: Deposition of C3b/iC3b leads to the concealment of antigens, immunoglobulins and...

Ngày tải lên: 09/08/2014, 07:20

... unit; BD, Behçet's disease; HLA, human leukocyte antigen; HO, heme oxygenase; PBMC, peripheral blood mononuclear cells; PMN, peripheral blood multinuclear cells Page of 10 (page number not for citation ... TLR4 cause reduction in HO-1 expression in peripheral blood mononuclear cells (PBMC), resulting in an augmentation of inflammatory responses in BD Page of 10 (page number not for citation purposes) ... in peripheral blood mononuclear cells (PBMCs) from patients with Behçet's disease (BD; n = 18) Values presented are mean and standard error of the mean (SEM) change, regarding to be the value of...

Ngày tải lên: 09/08/2014, 10:22

... Flow cytometry sorting of peripheral blood mononuclear cells (PBMC) subpopulations: CD3/CD19, CD14, and CD16 A-D Flow cytometry sorting of peripheral blood mononuclear cells (PBMC) subpopulations: ... and HER2 signal was detected in sorted subpopulations of Peripheral Blood Mononuclear Cells RNA from subpopulations of flow cytometry sorted cells was isolated and subjected to quantitative RT-PCR ... sensitivity of HER2 detection in blood, real time quantitative RTPCR was performed on RNA isolated from mock positive controls of ml of leukopak cells spiked with serial dilutions of breast cancer...

Ngày tải lên: 10/08/2014, 22:20

... %IL-17+ CD4+ T cells in CD4+ T cells Day0 IL-17+CD4+ Fold increase in the percentage of CD4+ IL-17+ T cells Page of 11 Figure Increased percentages of IL-17+ CD4+ T cells and IL-17+ CD8+ T cells in ... percentage of IL-23R+ T cells and percentage of IL-17+ T cells in SLE patients (a, c) Relationships between the percentage of IL-23R+ CD4+ T cells and the percentage of IL-17+ CD4+ T cells on day ... differentiation of Page of 11 T cells into Th17 cells [2] Taken together, the IL-23/ IL-17 axis significantly contributes to the pathogenesis of SLE In another autoimmune disease of chronic inflammatory...

Ngày tải lên: 12/08/2014, 15:22

... of hRasGRP4 expression in peripheral blood A, Evaluation of hRasGRP4 mRNA levels in unfractionated peripheral blood mononuclear cells (PBMCs), and PBMC-derived CD14+ cells, CD14-/CD3-/CD19- cells, ... unidentified population of peripheral blood mononuclear cells (PBMCs) express Ras guanine nucleotide releasing protein (RasGRP4) The aim of our study was to identify the cells in human blood that express ... expression of hRasGRP4 in the peripheral blood evaluated by immunoblotting A Immunoblotting of peripheral blood mononuclear cells from patients with RA and healthy controls Lysates were prepared from peripheral...

Ngày tải lên: 12/08/2014, 18:20

... changes in peripheral blood monocytes during progression of human immunodeficiency virus infection Effects of soluble immune complexes, cytokines, subcellular particulates from apoptotic cells, and ... characterization of changes induced by HAART on the broad immunenophenotype of the immune cells over time may facilitate the clarification of these mechanisms Although a considerable amount of work has ... software on an 8-bit pixel grey scale from 0–255 The dot intensity reflects cell binding density, which depends on both the level of expression of a particular antigen and the proportion of cells...

Ngày tải lên: 13/08/2014, 06:20

... tRNAPro in human peripheral blood mononuclear cells (PBMC) Infections were initiated with transfection supernatant containing approximately 200 pg of p24 antigen in a volume of 10 mLs of media, ... infectious of the Log 10 p24 ( pg/ml ) Retrovirology 2005, 2:21 16 24 32 Days post infection 40 tRNAPro2 human peripheral blood mononuclear cells complementary to the 3' 18 nucleotides of tRNAIle, ... Supernatants from transfected cells were assayed for HIV-1 p24 antigen (Beckman Coulter, Miami, FL) and infectivity [28] PBMC Infections Human peripheral blood mononuclear cells (PBMC) were collected,...

Ngày tải lên: 13/08/2014, 09:21

... distinct subsets of dendritic cells in human peripheral blood J Immunol 2000, 165:6037-6046 Sallusto F, Lanzavecchia A: Efficient presentation of soluble antigen by cultured human dendritic cells is ... Figure Phenotypic comparison of cells Phenotypic comparison of cells The expression of cell surface markers on (a) immature and (b) mature mDCs, moDCs and KG1 cells Immature cells were freshly isolated ... that peripheral blood mDCs have sixand five-fold lower protein content per cell than moDCs and KG-1 cells, respectively (data not shown) Unfortunately, the low numbers of mDCs in peripheral blood...

Ngày tải lên: 14/08/2014, 18:20

... augmented biofluids VEGF-A and CCL2 protein may be associated with increased survival duration of Indian ALS patients [9] We now measured the mRNA expression of VEGF-A and CCL2 in peripheral blood mononuclear ... Duration of disease is the interval between appearance of first symptom of ALS and collection of sample ALS subjects were asked to provide all clinical and demographic details at the age of disease-onset ... chemokine ligand-2; PBMCs, peripheral blood mononuclear cells levels did not vary between definite, probable and possible ALS cases (Figure 2B; p > 0.05) To find association of respiratory dysfunction,...

Ngày tải lên: 19/06/2014, 22:20

... PBMC profiles also might be useful in early detection of infections Conclusion The application of gene expression analysis to the study of human disease is a new and rapidly evolving area of investigation ... familiar to most users Reproducibility has been achieved Table Gene expression studies of peripheral blood mononuclear cells from patients with autoimmune diseases Subjects studied Gene arrays used Summary ... [15], although the advent of RNA stabilization tubes for blood collection may make this less of a concern in future studies Studies of the normal immune response Most sudies of autoimmune disease...

Ngày tải lên: 09/08/2014, 01:23

... obtained peripheral blood mononuclear cells (PBMCs) from RA patients and control individuals and examined the expression of miR-146a, miR-155, miR-132, miR-16, and miRNA let-7a Most of these ... Cell-Profiler image analysis software [25] siRNA transfection siRNAs targeting TRAF6 and IRAK-1 were transfected into THP-1 cells using Lipofectamine 2000 (Invitrogen, Carlsbad, Page of 10 (page ... effect of TNF-α stimulation on human PBMC GWB GWB staining using human PBMCs from a healthy donor, after hours stimulation with TNF-α (1 ng/ml), is shown Similar to THP-1 cells, the number of GWB...

Ngày tải lên: 09/08/2014, 13:21