object created as if by ca

... fraction (LV-EF) was calculated by the modified Simpson´s method LA and LV diameter were measured by M-Mode echocardiography High grade cardiac valvular disease was excluded by Color, PW and CW ... with significant differences between basal, midventricular and apical myocardium (see Table 3) Table 3: Tissue velocities (S´, E´, A´) of basal, mid and apical segments as assessed by VVI S´ ... analysis by VVI was feasible Segments were excluded if the endocar- http://www.medsci.org Int J Med Sci 2011, 109 dial border was not tracked properly, if the digital storage of cardiac cycles was...

Ngày tải lên: 25/10/2012, 11:15

... So-RNase HI It has been reported that Ec-RNase HI is stabilized by the Lys95 fi Gly [14] or Asp134 fi His [15] mutation by approximately °C in Tm at pH 5.5 Because Lys95 and Asp134 are conserved as ... such as Asp12, Glu50, Asp72 and Glu133, are clustered in the vicinity of Asp136 It has been reported that the corresponding mutation (Asp134 fi His) stabilizes Ec-RNase HI by 7.0 °C in Tm as a ... encoding R97G-RNase HI, D136H-RNase HI, 5·-RNase HI and 6·-RNase HI were constructed by sitedirected mutagenesis using PCR as described previously [33] Plasmid pET500M or pET500M4x was used as template...

Ngày tải lên: 18/02/2014, 13:20

... this was obtained as a crude extract from the leaves of Canavalia brasiliensis The extract enriched in arginase was used immediately for preparative-scale conversion of canavanine to canaline Canaline ... nm was measured Preparation of L-canaline Synthesis of radioactive canavanine from l-canaline was based on a previously described procedure [60] using [14C]cyanamide as a guanylating reagent As ... reagent As l-canaline is no longer commercially available, l-canavanine sulphate was converted to l-canaline by arginase treatment, essentially as described previously [61] The arginase required...

Ngày tải lên: 18/02/2014, 13:20

... 5¢-GATATTGACTG GCAATTCCCTGGTGGCGGC-3¢, and the reverse oligonucleotide sequences were 5¢-CAGCCGCCGTAGAAGTT GTAAGTCATCGCAAAG-3¢, 5¢-CGCCGCCACCAGGG AACATCCAGTCAATATCTAC-3, and 5¢-GCCGCCAC CAGGGAATTGCCAGTCAATATCTAC-3¢, ... concentrations were calculated directly by means of a pNP calibration curve in the case of the colorimetric assay, or by using a (GlcNAc)2 calibration curve in the case of quantitative HPLC-MS If pNP is ... al further hydrolyzed by the second type of enzymes – b-glucosaminidases – to yield GlcNAc as the final product Chitin catabolism through the carbohydrate catabolic cascade has rather complex signal...

Ngày tải lên: 20/02/2014, 01:20

... (5S)-[5-2H1]F420H2 yielded only[5–1H]F420 as indicated by the mass spectrum (Fig 2Bc), which was identical to that calculated for [5-1H]F420 (Fig 2Ba) This result can only be explained if FprA is Si-face specific ... of F420H2 oxidase H Seedorf et al indicated by the sequence motif H-X-E-X-D-X63-HX18-D-X62-H FprA is not phylogenetically related to any of the other F420-dependent enzymes and catalyses a reaction ... (B) Experiment with specifically 2H-labelled substrates showing that the mass spectrum of F420 formed from (5S)-[5-2H1]F420H2 by FprA-catalysed oxidation (c) is identical to the spectrum of [5-1H]F420...

Ngày tải lên: 20/02/2014, 03:20

... (RRKWRRWHL) [18] exhibit drastically different conformations In addition, pVIPR is bound in a canonical single conformation by B*2709, but in an exceptional dual conformation by the AS- associated B*2705 ... ⁄ D exchange may assist in the assignment of absorption bands arising from different secondary structure classes [27,30–33] By contrast, IR experiments in D2O can also complicate the interpretation ... to B*2709 by a hydrogen bond connecting pU5O7 and His116NE2, as indicated by a dashed red line Oxygen atoms are shown in red, nitrogen atoms in blue B*2705 binds the peptide in canonical conformation,...

Ngày tải lên: 06/03/2014, 00:21

... ⁄ 1029 ⁄ carotenoid 1000–1003 967–969 949–952 925–927 Key Raman linesb R1 R2 R3 R4 R5 Assignmentsc mC9=O, mC10a=O mC2a=O as mCaCm(a, b, c, d) as mCaCm(c, d) as mCaCm(a, b) mCbCb, s mCaCm(c), ... both cases, the Ca2 +-binding cavities are localized in the C-termini, which comprise O of Leu)4, Ser)5, Thr)6, OD1 of Asp)7 and OD1 and OD2 of Asp)13 in Fig 5A, O of Val)3, Leu)4, Ser)5, Thr)6, Asp)7 ... members such as the Ca2 +-ATPase from skeletal muscle sarcoplasmic reticulum whose transmembrane helices tilt approximately 30° when transformed from the Ca2 +-bound form (E 1Ca2 +) to the Ca2 +-free...

Ngày tải lên: 30/03/2014, 02:20

... intracellular calcium, as measured by Fura ⁄ AM-based calcium imaging, together with a sustained increase in Ins(1,4,5)P3, which peaked later than calcium (Fig 2Aa) By contrast, when Ca2 +-free BSS was Fig ... indicate that transient calcium increases result from the release of Ca2 + from intracellular stores, whereas subsequent sustained calcium increases reflect calcium entry, including the so-called ... to calcium or incapable of retaining it By contrast, once the calcium-dependent process has been activated by both receptor and calcium, the effects of calcium are retained, even after the calcium...

Ngày tải lên: 30/03/2014, 03:20

... upon increase of |r| was primarily associated with a decrease of the optical factor, which for the pitch angle 50° was as low as 0.12 However, electron microscopy data established that if the superhelix ... well with the DNA titration curve by ethidium bromide (Fig 1) The differences can be explained by a different enzyme mechanism of action As in case of topoisomerase I we observed only initial and ... anticancer drugs Experimental procedures Harvesting and purification of plasmid DNA Ecolab DH5a strain was used for pUC19 amplification A standard method of lyses by alkali [44] was used for plasmid...

Ngày tải lên: 30/03/2014, 11:20

... (Table 2) Human ADH3 was found to readily reduce GSNO with NADH as coenzyme yielding a kcat/Km of 90 000 min)1ÆmM)1 Reductive capacity for GSNO was drastically reduced by the substitution of ... ND 110 ± 16 ND ND 30 >5000 a kcat (min)1) Km (lM) kcat/Km (min)1ÆmM)1) Km (lM) kcat (min)1) kcat/Km (min)1ÆmM)1) Km (lM) kcat (min)1) kcat/Km (min)1ÆmM)1) NADPH kcat (min)1) To determine the structure ... should be regarded with caution For mass spectrometric analyses, reduction of GSNO was performed in 10 mM ammonium bicarbonate pH 7.5, as phosphate ions severely impair mass spectrometric analysis...

Ngày tải lên: 31/03/2014, 07:20

... hydrolysis was followed by monitoring the fluorescence released from the beads as the CFPs were cleaved in their substrate linker Percentage of hydrolysis was calculated as the ratio of released CFPs ... the last years, evidence has been mounting that support a role for hK2 in metastasis and cancer progression by virtue of its in vitro proteolysis of several biological substrates involved in cancer ... tag (8) The CFP-X5-his protein was fixed to Ni2+-nitrilotriacetic acid magnetic beads and (9) treated by the protease hK2 The released CFP fluorescent protein was measured with a fluorescence reader...

Ngày tải lên: 31/03/2014, 23:20

... until November 2005 Measures The Inventory for Assessing the Quality of Life (ILC) This measure was developed in Germany by Mattejat and colleagues as a short and practical assessment tool for ... the outcome variable However, none of the covariates was significant Because the QoL measures in the sample were only minimally influenced by differences between grade cohorts, all following analyses ... male:female and urban:rural ratios as well as age range Because the QoL measures in the sample were only minimally influenced by differences between grade cohorts, statistical analyses could be conducted...

Ngày tải lên: 18/06/2014, 22:20

... has been fraught with difficulties due to uneven qualities of the pathology Diffusion Tensor Imaging (DTI) has emerged as a powerful and sensitive tool to analyze white matter disease Specifically, ... groups, was apparent in EAE mice as evidenced by the intensity changes in radial and axial diffusivity maps (Figure 2B) Statistical analysis of changes in radial diffusivity failed to show significant ... transfer, as a function of axial diffusivity VLWM and injured VLWM Both analyses showed a statistically significant linear correlation of clinical score during the recovery phase with axial diffusivity...

Ngày tải lên: 19/06/2014, 22:20

... no significant difference occurred (p = 0.9) Latissimus Control Shoulders Dorsi itself was activated significantly earlier than Biceps (p = 0.033) The onset of Biceps activity was significantly ... significant differences Paired t-tests were undertaken to evaluate if any specific differences occurred between the individual muscles and injured and non-injured limbs Paired t-tests indicated ... placement was carried out by noting EMG activity during resisted protraction of the arm at 90 degrees flexion Infraspinatus (see figure 2) Following identification of the spine of the scapula, two...

Ngày tải lên: 20/06/2014, 04:20

... non-significant difference between parent report of students and patients and might be interpreted mistakenly Given non-significant differences between parent proxy Page of reports and significant differences ... mental health or psychopathological aspects, at least when measured as parent reported emotional and behavioural problems on the CBCL The child’s QoL as measured by the ILC refers to subjective ... 0-7 scale score indicates the number of life domains perceived as problematic (0 = no problem on any life domain, = problems on all life domains) We used the ILC because in this study it has certain...

Ngày tải lên: 20/06/2014, 15:20

... There was no significant difference in the measured EF, which was 52.93 ± 3.10% in conventional pigs and 59.00 ± 5.56% in micropigs Furthermore, the EF measured by MDCT was not significantly different ... rapid technical developments in scanner hardware have led to improvements in spatial and temporal resolution, as well as led to significantly faster cardiac scans Consequently, MDCT has become ... expressed as mean ± SD Where appropriate, categorical variables were compared using a Wilcoxon test A p-value ＜ 0.05 was considered significant Results The end-systolic and end-diastolic phase images...

Ngày tải lên: 07/08/2014, 20:23

... Plant mass had no statistically significant effect on biomass allocation (P > 0.05) except for leaves (F-Ratio = 5.50; P = 0.0236) Relative allocation to leaves tended to increase as plant mass ... full-light weeded Different letters in a row indicate statistically significant differences (LSD procedure, 95% confidence interval) * Soil water content was computed as the mean of weekly measurements ... mean seedling biomass was 9.87 g (shoot), 7.10 g (roots) and 16.97 g (total) for a random subset of 33 seedlings Different letters in a row indicate statistically significant differences (LSD procedure,...

Ngày tải lên: 08/08/2014, 00:21

... chronologies showed no significant correlation This phenomenon indicates that different factors affect cambial activity, expressed by ring width, and differentiation expressed by density cell Climate-growth ... explained by climate Recently, climatic impact on ring width has been decreasing, but climatic impact on maximum density has been increasing From 1931 to 1960, ring width was affected by summer ... the period from 1961 to 1990, the temperature of June alone was significant Maximum latewood density was significantly influenced by temperature in early spring (April/May) and late summer (August)...

Ngày tải lên: 08/08/2014, 19:21

... Black Forest by measurement of the different fluorescence signatures of needles during a yr period from 1987 to 1988 The differentiation between photochemical Qquenching and non-photochemical E-quenching ... damage class 3/4) were measured using different fluorescence methods 1) The red laser-induced chlorophyll fluorescence kinetics (determination of Rfd-values as a vitality index of needles) measured ... apparatus as seen from the lower Rfd-values In contrast, the Rfd-values as a vitality index and as an index of the intactness of the photosynthetic apparatus, showed a different behavior There was a...

Ngày tải lên: 09/08/2014, 04:20

... Vol 11 No Catterall et al Asp) within matrix proteins could also interfere with the normal turnover of cartilage as certain proteases, including matrix metalloproteinase-3 and some caspases, are ... non-arthritic cartilage can be explained, as shown previously [24], by the reciprocal rise in these cartilages of the very long-lived racemized aspartate (D-β-Asp) which is not recognized by the protein-iso- ... available Significantly more IsoAsp and GAG were in the deep non-lesioned OA cartilage compared with the superficial non-lesioned OA cartilage (Table and Figure 3) There was no significant difference...

Ngày tải lên: 09/08/2014, 14:20