o2 and other reactive oxygen species ros lipid peroxidation lpo intracellular ca2 ca2 i mitochondrial ca2
Induction of anti tumor response by dendritic cell based vaccination
Ngày tải lên :
16/09/2015, 15:54
... can inhibit the proliferative activity of FL [100-102] In addition to in vitro condition, the in vivo haematopoietic effects of FL have also been investigated Administration of FL in mice induces ... vaccine In conclusion, we devised two strategies of inducing DC to prime muc-1 specific anti-tumor response in vivo It is suggested that the availability of DC at the antigen site (in situ) is ... peptide 96 3.1.11 Induction of muc-1 specific antibody response via in vivo priming with pNGVL3-hFLex plasmid DNA and muc-1 peptide 97 3.1.12 Induction of muc-1 specific anti-tumor response via in...
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Báo cáo y học: "Mechanisms of HIV non-progression; robust and sustained CD4+ T-cell proliferative responses to p24 antigen correlate with control of viraemia and lack of disease progression after long-term transfusion-acquired HIV-1 infection" pdf
Ngày tải lên :
13/08/2014, 05:21
... 121 122 123 Figure 20.3 years post infection) to Gag peptide epitopes by peptide pool mapping in an individual with increasing viraemia (C122, Identification of responses Identification of responses ... images are shown Individual peptides intersecting positive peptide pools containing HLA-relevant epitopes (Additional file 2) were then tested individually, and positive responses indicated by dark ... coinciding with weakening proliferative responses that gradually became negative A similar correlation between anti viral immune responses and a spike in viral replication was demonstrated in...
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More Show Me How: Everything We Couldn't Fit in the First Book Instructions for Life from the Everyday to the Exotic Perfect Paperback
Ngày tải lên :
15/01/2014, 12:15
... skin) tsp evening primrose oil (dry skin) tsp witch hazel (oily skin) whip up facial moisturizer lift wax from a carpet tsp distilled water tbsp cocoa butter tbsp beeswax Melt in a double boiler ... bear wedding rings and coins Hire lucky lion dancers to entertain guests An heirloom rosary is part of the bride’s bouquet A veil and cord are placed on bride and groom to symbolize their unity The ... the baby aisle! Flirt by the fruit stand How ripe is that banana? Mingle with other singles by the frozen-dinner aisle If his cart is full of family or feminine items, steer clear—he’s taken 142...
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Tài liệu Báo cáo khoa học: Identification of different isoforms of eEF1A in the nuclear fraction of human T-lymphoblastic cancer cell line specifically binding to aptameric cytotoxic GT oligomers ppt
Ngày tải lên :
21/02/2014, 00:20
... displays specificity in recognizing GT oligomers, and the selective cytotoxic action on CCRF-CEM cells suggests a possible role for eEF1A in maintaining the viability and proliferative activity ... high basic proteins it might be that either methylation of the lysine or specific substitutions in the amino acid sequence, increasing the number of basic residues of lysine, arginine and histidine, ... oligonucleotides simply on the basis of a charge increase at specific amino acids in its nucleotidebinding site, but not its binding selectivity for the GT sequences Different post-translational modifications...
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Báo cáo khoa học: Protein kinase Ch activity is involved in the 2,3,7,8tetrachlorodibenzo-p-dioxin-induced signal transduction pathway leading to apoptosis in L-MAT, a human lymphoblastic T-cell line potx
Ngày tải lên :
23/03/2014, 13:20
... IUÆmL)1 penicillin, and 0.1% (v ⁄ v) streptomycin at 37 °C in 95% air and 5% CO2 Jurkat T cells were maintained under the same conditions in RPMI 1640 of similar composition, pH 7.4, containing ... Bitterlich G, Uberall F, et al (1999) Synergistic action of protein kinase C theta and calcineurin is sufficient for Fas ligand expression and induction of a crmA-sensitive apoptosis pathway in ... of an activation-induced cell death mechanism (AICD) involved in T-cell apoptosis [18] Furthermore, activation of PKCh and its kinase activity have been implicated in the AICD mechanism in human...
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báo cáo hóa học:" Protective CD8+ T-cell responses to cytomegalovirus driven by rAAV/GFP/IE1 loading of dendritic cells" pdf
Ngày tải lên :
18/06/2014, 15:20
... killed, indicating strong antigen specificity for the CTLs generated by AAV/IE1 loading DCs at day Alternately, the addition of anti-class I antibodies significantly inhibited the killing activity ... manuscript WMK participated in study design and coordination and revised and drafted the manuscript EC participated in study design and coordination and revised the manuscript MCI carried out ... K, Jonjic S, Luske A, Koszinowski UH: Interstitial murine cytomegalovirus pneumonia after irradiation: characterization of cells that limit viral replication during established infection of the...
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báo cáo hóa học:" Comparative study on the immunogenicity between an HLA-A24-restricted cytotoxic T-cell epitope derived from survivin and that from its splice variant survivin-2B in oral cancer patients" pot
Ngày tải lên :
18/06/2014, 15:20
... mitotic checkpoint [21], and anti-apoptotic function is lost in some splicing variants such as survivin-2B, in which BIR domain is disrupted by the insertion of exon 2B [17] Survivin-2B and other ... against survivin-positive cancer cell lines Induction Induction of survivin-C58 peptide-specific CTLs and their cytotoxicity against survivin-positive cancer cell lines CTLs were induced from ... peptides derived from survivin and its splicing variant survivin-2B were examined for HLA-A24-binding affinity and immunogenicity [22] It was shown that Survivin-2B80-88 peptide (amino acid sequence...
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báo cáo hóa học:" Regulatory activity of azabisphosphonate-capped dendrimers on human CD4+ T cell proliferation enhances ex-vivo expansion of NK cells from PBMCs for immunotherapy" potx
Ngày tải lên :
18/06/2014, 15:20
... http://www.translational-medicine.com/content/7/1/82 Specific and competitive interaction of azabisphonate dendrimers with pure CD4+ T cells Figure Specific and competitive interaction of azabisphonate dendrimers ... haploidentical individuals presenting a functional mismatch in the NK cell repertoire towards recipients MHC -I ligands In this context, NK cell alloreactivity has been shown to increase prognosis ... Performing an equilibrium binding assay coupled with flow cytometry analysis, we revealed a specific interaction signature of 3a-G1-Julo with purified CD4+ T-cells After incubation with increasing...
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Báo cáo hóa học: " A quantitative real time PCR method to analyze T cell receptor Vb subgroup expansion by staphylococcal superantigens" doc
Ngày tải lên :
18/06/2014, 16:20
... uniform and high efficiency and linearity The specificity of qRT-PCR using SYBR Green I platform was often determined by analyzing melting curves In this study, the specificities of each primer ... uniform and high efficiency to achieve a valid quantification The efficiency and linearity of primers could be assessed by analyzing the slope and R2 value of the standard curve, respectively In ... expanded in this study As shown in Table 4, the Vb specificities of SAgs observed in this study was very similar to those described in previous studies with minor variation [7,11,12,24-26] In...
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Báo cáo hóa học: "Comparison of anti-CD3 and anti-CD28-coated beads with soluble anti-CD3 for expanding human T cells: Differing impact on CD8 T cell phenotype and responsiveness to restimulation" pot
Ngày tải lên :
18/06/2014, 16:20
... and CD8 + Naive T Cell Isolation Kits purchased from Miltenyi Biotech Monitoring T cell division and early expansion using CFSE labeled cells To monitor cells division and expansion during the early ... proliferation began) was similar in unstimulated, bead-stimulated, and anti-CD3-stimulated wells indicating neither stimulus caused extensive early activation-induced cell death (AICD) (Figure ... anti-CD3 gave qualitatively identical results Comparison of T cell size after stimulation with anti-CD3/ CD28 beads or anti-CD3 To gain additional insight into the relative impact of beads and...
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Báo cáo hóa học: "IL-2 as a therapeutic target for the restoration of Foxp3+ regulatory T cell function in organ-specific autoimmunity: implications in pathophysiology and translation to human disease" doc
Ngày tải lên :
18/06/2014, 16:20
... NOD mice This resistance is associated with significantly reduced insulitis scores and frequencies of IFN-g, TNF-a and IL-17 secreting autoreactive CD4+ T cells, and correlates with increased IL-2 ... importance of IL-2 in the maintenance of self-tolerance [16] Il2 allelic variation (Idd3) and resistance to autoimmunity in NOD mice T1 D susceptibility is inherited through multiple genes, with a strong ... organ-specific autoimmunity: implications in pathophysiology and translation to human disease Eva d’Hennezel1†, Mara Kornete1†, Ciriaco A Piccirillo2* Abstract Peripheral immune tolerance requires a finely...
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Báo cáo sinh học: " Thottapalayam virus is genetically distant to the rodent-borne hantaviruses, consistent with its isolation from the Asian house shrew (Suncus murinus)" potx
Ngày tải lên :
18/06/2014, 18:20
... sensitive and specific molecular detection assays for use in screening cases of acute febrile illness in Asia, and insectivore investigations to provide insight into the public health significance ... recent serosurvey in Tamil Nadu identified the presence of hantavirus IgM positivity in some human febrile illness cases [19,20] In addition, anti-TPM virus antibodies were detected in sera from ... hantaviruses in India, Thailand and Indonesia, it is unclear whether these represent TPM virus or other hantavirus infections The determination of the entire genome sequence of TPM virus will allow...
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Báo cáo sinh học: "The combined transduction of copper, zincsuperoxide dismutase and catalase mediated by cell-penetrating peptide, PEP-1, to protect myocardium from ischemia-reperfusion injury" ppt
Ngày tải lên :
18/06/2014, 19:20
... details Institute of Clinical Medicine and Department of Cardiology, Renmin Hospital, Hubei University of Medicine, Shiyan, Hubei 442000, China Department of Critical Care Medicine, Renmin Hospital, ... from the reaction of MDA with 2-thiobarbituric acid The CK and MDA biochemical analyses were performed using commercial kits (JianCheng Bioengineering Institute, China) CK-MB activity and cTnT levels ... amphipathic a-helical structure on the membrane These characteristics are similar to those of cationic antimicrobial peptides involved in host innate immunity, suggesting that PEP-1 can kill microbes...
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Báo cáo hóa học: " Thottapalayam virus is genetically distant to the rodent-borne hantaviruses, consistent with its isolation from the Asian house shrew (Suncus murinus)" pdf
Ngày tải lên :
20/06/2014, 01:20
... sensitive and specific molecular detection assays for use in screening cases of acute febrile illness in Asia, and insectivore investigations to provide insight into the public health significance ... recent serosurvey in Tamil Nadu identified the presence of hantavirus IgM positivity in some human febrile illness cases [19,20] In addition, anti-TPM virus antibodies were detected in sera from ... hantaviruses in India, Thailand and Indonesia, it is unclear whether these represent TPM virus or other hantavirus infections The determination of the entire genome sequence of TPM virus will allow...
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Báo cáo hóa học: " Impairment of the CD8+ T cell response in lungs following infection with human respiratory syncytial virus is specific to the anatomical site rather than the virus, antigen, or route of infection" pdf
Ngày tải lên :
20/06/2014, 01:20
... [8] In addition, following tail skin scarification of mice with the same virus, no viral DNA was detected in various lymph nodes distant from the site of initial infection by a highly sensitive ... dermal (ID inoculation with VV-M2), or disseminated (IN inoculation with VV-M2) infection were present in the lungs and were impaired in secretion of IFNγ, irrespective of the type and site of infection ... in the spleen under conditions of local infection in the lung (i. e influenza, RSV), localized dermal infection (i. e VV-M2 administered ID), or disseminated infection (i. e VV-M2 administered IN)...
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Báo cáo hóa học: " Characterization of the IFN-γ T-cell responses to immediate early antigens in humans with genital herpes" doc
Ngày tải lên :
20/06/2014, 01:20
... Low/high recurrence ratio including HSV-1 single positives Low/high recurrence ratio without HSV-1 single positives ilar trend of increasing within individuals with less severe disease In contrast ... AR, Allison AC: Vaccinating guinea pigs with recombinant glycoprotein D of herpes simplex virus in an efficacious adjuvant formulation elicits protection against vaginal infection Vaccine 1994, ... versatile assay[16,17] The IFNγ ELISPOT assay is able to measure immune responses with high precision and sensitivity, and is ideal for screening purposes The assay identifies T-cells that recognize...
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báo cáo hóa học:" Metabolic and anthropometric parameters contribute to ART-mediated CD4+ T cell recovery in HIV-1-infected individuals: an observational study" potx
Ngày tải lên :
20/06/2014, 08:20
... that high adiposity (which is normally associated with insulin resistance, dyslipidemia and atherogenesis) might be beneficial for HIV-infected individuals, contributing to lower steady state viral ... Apolipoprotein A-1 interacts with the N-terminal fusogenic domains of SIV (simian immunodeficiency virus) GP32 and HIV (human immunodeficiency virus) GP41: implications in viral entry Biochem Biophys ... chronic inflammation and increased immune activation, with alteration of metabolic parameters associated with lipid metabolism and increased atherogenic risk (as assessed by increased carotid intima-media...
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Báo cáo sinh học: "Life and death as a T lymphocyte: from immune protection to HIV pathogenesis" doc
Ngày tải lên :
06/08/2014, 19:21
... RJ, Miedema F, Hamann D: T-cell division in human immunodeficiency virus (HIV)-1 infection is mainly due to immune activation: a longitudinal analysis in patients before and during highly active ... replication [4] In fact, the level of immune activation during HIV infection is a better predictor of disease progression than is viral load. This chronic generalized immune activation is characterized ... association between HIV infection and immune activation The first is that T cell homeostasis is disrupted by the chronic activation of the innate immune system by HIV infection The cells of the innate...
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