nucleotide release by airway epithelia

... TNF- LPS Figure production of IL-6 and IL-8 by airway epithelial cells as a function of arginine Enhanced Enhanced production of IL-6 and IL-8 by airway epithelial cells as a function of arginine ... concentration may deviate from that of the micro-milieu surrounding the airway epithelial cells Enzymes expressed by airway epithelial cells of patients with asthma and that catabolise arginine ... arginase and iNOS activities by airway epithelial cells Therefore, polycationic proteins in the airways of asthmatics may give rise to exaggerated IL-6 and IL-8 responses by both the arginine uptake...

Ngày tải lên: 12/08/2014, 14:20

... IL-8 release could be detected in the Figure by human lung epithelial cells S pneumoniae induced JNK-dependent IL-8 and IL-6 release S pneumoniae induced JNK-dependent IL-8 and IL-6 release by ... pattern of immunomodulatory factors released by cultured lung epithelial BEAS cells in vitro may indicate that activation of tracheobronchial epithelial cells by pneumococci in vivo impact on immune ... growth factors by human lung epithelial cells BEAS-2B cells were infected with S pneumoniae strain R6x (106 cfu/ml) for 5, 10, or 20 h Cytokine release in the supernatant was measured by Bioplex...

Ngày tải lên: 12/08/2014, 16:20

... that HAT might mediate airway inflammation by PAR-2 activation In addition to airway inflammation, hypersecretion of airway mucus is a characteristic sign of chronic obstructive airway diseases, which ... in patients with chronic airway diseases [13–15] In a previous study [16], HAT was shown to increase MUC5AC gene expression, leading to mucus production by the airway epithelial cell line (NCI-H292) ... the mechanism by which HAT induces AR release remains unknown In the present study, HAT-induced AR release and the target molecule of HAT were investigated Results HAT regulates AR release at the...

Ngày tải lên: 30/03/2014, 11:20

... IFN-γ-induced protein expression in human airway epithelial cells Cigarette Smoke Extract Causes Minimal Airway Epithelial Cell Cytotoxicity The effects of epithelial cell exposure to CSE on defense ... inhibit effects of cigarette smoke on airway defense by restoring IFN-γ-dependent antiviral effects Discussion Epithelial cells in the airway are often targeted by respiratory viruses, and these ... effects on IFN-γ-dependent antiviral mechanism in airway epithelial cells that would impair host defense In this report, we use primary human airway epithelial cells and an extract of cigarette smoke...

Ngày tải lên: 12/08/2014, 11:22

... transcription by airway epithelial cells following stimulation with PCBG IL-8 secretion by PCBG stimulated airway epithelial cells is mediated by MAP Kinases Figure PCBG induces IL-8 release from ... of IL-8 by airway epithelial cells stimulated with PCBG requires intra-cellular, rather than extra-cellular, calcium mobilization IL-8 secretion by airway epithelial cells is mediated by NFκB ... in cytokine signaling by airway epithelial cells activated with PCBG [17,18] Herein, we demonstrate that 1HAEo- human airway epithelial cells simulated with PCBG induce the release of the neutrophil...

Ngày tải lên: 12/08/2014, 11:22

... baseline release nor IL-1b stimulated release of IL-6 (P = NS; Figure 4) IL-8 (ng/ml) B IL-1 CSA * Both † 100 50 Vehicle IL-1 CSA Both Figure Secretion of IL-8 by transplant-derived airway epithelial ... transplant-derived airway epithelial cells Airway epithelial cells have been shown to contain cyclophilin, the cytosolic receptor for cyclosporine, [13] and thus may regulate epithelial function ... lung transplant recipients and the use of primary human airway epithelial cells collected by bronchoscopy in these patients were approved by the University of Chicago Institutional Review Board...

Ngày tải lên: 12/08/2014, 13:22

... hepcidin in the airway by inflammatory stimuli We were able to demonstrate that hepcidin was expressed locally by airway epithelial cells, and that its expression can be regulated by the pro-inflammatory ... metabolism in the lung requires coordination between epithelial cells and macrophages [25], we next evaluated whether hepcidin released by airway epithelial cells could impact iron transport in Figure ... < 0.05 Results Hepcidin is expressed in airway epithelial cells and is regulated by IFN-g To determine whether hepcidin is expressed locally in airway epithelial cells and is regulated in response...

Ngày tải lên: 12/08/2014, 13:22

... in airway epithelial cells of small airway origin We hypothesized that primary human small airway epithelial cells (SAECs) would produce detectable NO gas phase release following stimulation by ... of the smaller airways The phenotypic features of the airway epithelium change with increasing airway generation For example, as airway generation increases (smaller diameter airways), the epithelium ... http://respiratory-research.com/content/10/1/3 stimuli in a pattern distinct from epithelial cells of large airway origin [8] Materials and methods Cell culture Cryopreserved passage small airway epithelial cells (SAECs) from different...

Ngày tải lên: 12/08/2014, 14:20

... as Hsp72, released by RSV-infected airway epithelial cells recruit and activate neutrophils in the lower airways [21] Accordingly, we hypothesized that (i) RSV infection results in the release ... assessed by one-way analysis of variance (ANOVA) Differences identified by ANOVA were pinpointed by Student-NewmanKeuls' multiple range test Results RSV infection induces Hsp72 expression in airway epithelial ... appear to directly activate neutrophils in the lower airways [21] Locally produced cytokines or other molecules released by virally infected airway epithelial cells are likely responsible for recruiting...

Ngày tải lên: 12/08/2014, 14:20

... well documented that epithelial cells, including those of the airways, produce and release growth factors [17], the mechanism, or mechanisms, regulating cytokine-induced release of growth factors ... region of airway epithelial cells, where it is cleaved by ADAM17 Fig 6b illustrates the timing of this inducible translocation, with an increase in TGFα near the apical surface observed by 30 – ... constitutive release of TGFα may be important for general maintenance of an epithelial barrier, it is the inducible nature of TGFα redistribution that likely contributes to the role of airway epithelial...

Ngày tải lên: 12/08/2014, 15:20

... small airway epithelial cells Cigarette Cigarette smoke extract caused necrosis with no or little evidence of apoptosis in primary human small airway epithelial cells (SAEC) Primary human small airway ... smoke on oxidative stress and proinflammatory cytokine release in primary human airway epithelial cells and in a variety of transformed alveolar epithelial cells Respir Res 2006, 7:132 Publish with ... evidence of apoptosis in primary human small airway epithelial cells (SAEC) Primary human small airway epithelial cells (SAEC) were treated with media alone (control) and various concentrations of CSE;...

Ngày tải lên: 12/08/2014, 15:21

... effected by corticosteroids exposure and may be implicated as a potential mechanism of viral persistence in the airway epithelium Steroid treatment prolonged virus release from airway epithelial ... DR4/DR5 expression and apoptosis of infected epithelial cells has a role in viral infections by Guinea pig airway epithelium and how this may be altered by corticosteroid exposure This objective ... http://respiratory-research.com/content/7/1/78 Figure Representative images of airway epithelial immunostaining of Cam Hartley Guinea pigs Representative images of airway epithelial immunostaining of Cam Hartley Guinea pigs...

Ngày tải lên: 12/08/2014, 16:20

... epithelial cells and in primary human small airway epithelial cells CSE differentially induced cell death in a concentrationdependent manner in various epithelial cell lines measured by LDH release ... small airway epithelial cells Figure 10 Cigarette smoke extract treatment caused NF-κB RelA/p65 nuclear translocation in primary human small airway epithelial cells Primary human small airway epithelial ... alveolar epithelial cell lines as well as in primary human small airway epithelial cells However, CSE triggered NF-κB activation and pro-inflammatory cytokine release in primary human small airway epithelial...

Ngày tải lên: 12/08/2014, 16:20

... human alveolar epithelial type II cells Am J Physiol Lung Cell Mol Physiol 2006, 290:242-249 Jiang X, Ingbar DH, O’Grady SM: Adrenergic stimulation of Natransport across alveolar epithelial cells ... due to other infectious and non-infectious etiologies then this is another potential mechanism by which beta-agonists might improve fluid clearance and thus support the findings of our study ... Newland P, Diver M, Southern KW: Pulmonary edema in meningococcal septicemia associated with reduced epithelial chloride transport Pediatric Critical Care Medicine 2006, 7:119-124 Page of (page number...

Ngày tải lên: 12/08/2014, 23:23

... perinuclear of A549 cells by fluorescence microscope and laser scanning confocal microscope 10 Acknowledgements This work was partly supported by Project (no 81071869) supported by the National Natural ... Arg-Eu-HAP were synthesized by hydrothermal method Aqueous solution with calcium nitrate Ca(NO3)2·4H2O The cytotoxicity of Arg-Eu-HAP was evaluated using flow cytometry in human lung epithelial (A549) ... the HAP surface could be replaced by the other metal cations with similar ionic radii, especially lanthanide ions The formation of Eu-doped HAP could be confirmed by the luminescence study The luminescence...

Ngày tải lên: 20/06/2014, 22:20

... MMP-3 by pro-MMP-3 assay To determine whether the up-regulation of the expression of MMP-3 gene by protein I/II stimulation in RA FLSs resulted in changes in gene translation and thereby contributed ... MBZ is supported by a grant from the French Ministry of Research and Technologies, and VAD, by grants from Région Alsace, Amgen, and Wyeth Lederle This work was supported in part by a grant from ... RA and OA FLSs in response to protein I/II has been previously shown to be followed by an increase of IL-6 release from these cells (MB Zeisel and colleagues, unpublished data) [20] Since we...

Ngày tải lên: 09/08/2014, 06:22

... performed by MK All experiments were performed by RB and MS except the culture of synoviocytes, which was performed by DP and TS Supervision was fulfilled by AS and PT, with daily supervision by RN ... determined by ELISA IL1β was obtained from Roche Diagnostics (Mannheim, Germany) Collection of the synovial biopsy and culture of FLS Synovial tissue was collected from an actively inflamed joint by ... microparticles were isolated from ml of synovial fluid by centrifugation for hour at 17,570 g and 20°C Supernatant (975 µl) was removed and replaced by 975 µl of PBS containing trisodium citrate Microparticles...

Ngày tải lên: 09/08/2014, 06:22

... the in vivo role of airway epithelial TLR2 signaling in SPLUNC1 production was not addressed in the current study This can be done by overexpressing TLR2 exclusively in airway epithelial cells of ... human and mouse airway epithelial cell cultures to determine if TLR2 signaling is involved in SPLUNC1 expression following Mp infection or TLR2 agonist stimulation We found that airway epithelial ... study were covered by a protocol approved by our Institutional Animal Care and Use Committee Briefly, tracheas from mice were isolated and digested with 0.1% protease, and the released cells from...

Ngày tải lên: 12/08/2014, 11:23

... to release Ca2+ into the cytosol is influenced, in part, by the cytosolic local Ca2+ concentration, a confined discharge of Ca2+ by NP-EGTA photolysis induced an activation of Ca2+ release by ... of localized Ca2+ mobilization by Ca2+ caged Determination of localized Ca2+ mobilization by Ca2+ caged technique A Confocal XY images illustrating Ca2+ release by photolysis of NP-EGTA molecule ... 3A–C) To prevent the release of Ca2+ by IP3Rs from the ER, we measured the response that had been only induced by the flash We treated cells h with 10 μM thapsigargin (TG) to release the whole Ca2+...

Ngày tải lên: 12/08/2014, 14:20

... Chlorine causes airway injury associated with increase in airway responsiveness to methacholine and airway inflammation γδ T cell deficient mice shed more epithelial cells but have no airway hyperresponsiveness ... airway epithelial damage and the degree of BAL neutrophilia [7] A murine model (A/J strain) of irritant induced asthma caused by acute chlorine exposure [8] showed a significant increase in airway ... post-exposure that correlated with airway epithelial damage and shedding Furthermore, this study provided evidence of oxidant stress and nitrosylation of proteins in airway epithelial cells and alveolar...

Ngày tải lên: 12/08/2014, 15:20