nuclear transplantation in mammalian eggs and embryos
Báo cáo khoa hoc:" CP-31398, a putative p53-stabilizing molecule tested in mammalian cells and in yeast for its effects on p53 transcriptional activity" potx
Ngày tải lên :
11/08/2014, 08:20
... lacking histidine and containing 20 mM 3-AT (figure 1C) In contrast, expression of three mutant http://www.jnrbm.com/content/3/1/5 forms of p53 [1] with point mutations in their DNA-binding domain ... stabilizing action of CP31398 in yeast cells and in human cells CP-31398, a compound isolated in an antibody-based in vitro screen, was reported to stabilize the p53 DNA-binding core domain and to ... CH: Latent and active p53 are identical in conformation Nat Struct Biol 2001, 8:756-60 Espinosa JM, Emerson BM: Transcriptional regulation by p53 through intrinsic DNA/chromatin binding and site-directed...
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Tài liệu Báo cáo khoa học: Nuclear receptors in the mosquito Aedes aegypti Annotation, hormonal regulation and expression profiling ppt
Ngày tải lên :
18/02/2014, 13:20
... code for a single protein Interestingly, during the initial process of identifying the NR family members in the A aegypti genome, we predicted two isoforms of AaFTZ-F1, differing only in their A ... Ets-domain protein and Broadcomplex, C2H2-type zinc-finger DNA-binding protein [18,19] Despite the achievements mentioned so far, there are additional NRs that remain uncharacterized in the mosquito, ... castaneum and A aegypti as well as the sequences of the human orthologs When different isoforms were recovered, only the longest amino acid sequences that included the DNA binding, hinge and ligand-binding...
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Báo cáo khoa học: Nuclear localization of human spermine oxidase isoforms – possible implications in drug response and disease etiology pptx
Ngày tải lên :
07/03/2014, 05:20
... overexpressing SMO cells further confirmed the increased SMO activity, with decreases in intracellular spermine pools and increased spermidine levels in SMO1 and SMO5 cells These changes in spermine and ... oxidation of spermine to spermidine diminishes nuclear spermine pools Because spermine has essential roles in the the protection of DNA, including free radical scavenging and DNA shielding, this reduction ... media (Mediatech, Inc., Herndon, VA, USA) containing 9% iron-supplemented fetal bovine serum (Hyclone, Logan, UT, USA) and 1% penicillin and streptomycin Beas2B cells were maintained in LHC-9 serum-free...
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Báo cáo khoa học: The fabp4 gene of zebrafish (Danio rerio) ) genomic homology with the mammalian FABP4 and divergence from the zebrafish fabp3 in developmental expression pot
Ngày tải lên :
07/03/2014, 10:20
... Gutierrez-Gonzalez LH & Hamilton JA (2003) Intracellular lipid binding proteins: evolution, structure and ligand binding In Cellular Proteins and Their Fatty Acids in Human Health and Disease (Duttaroy AK & ... structure and ligand-binding specificity and affinity of FABP3 and FABP4, the transcripts and proteins of the two paralogous genes exist in multiple tissues and are colocalized in several mammalian ... hpf), intestine (In, 48 hpf), and intestinal bulb (Inb, 48 hpf) The relative positions of the notochord (No) and otic vesicle (Ov) are indicated in red (A5, A10) (B) fabp4 mRNA was distributed in...
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GEOGRAPHIC VARIATION IN U.S. THYROID CANCER INCIDENCE, AND A CLUSTER NEAR NUCLEAR REACTORS IN NEW JERSEY, NEW YORK, AND PENNSYLVANIA potx
Ngày tải lên :
15/03/2014, 01:20
... Changing trends in incidence and mortality in Scotland Clinical Endocrinology 62(2):156-162, 2005 Leenhardt L., Grosclaude P., and Cherie-Challine L Increased incidence of thyroid carcinoma in ... presented in this report indicate that emissions from nuclear power reactors are likely to be a contributing factor in current U.S thyroid cancer incidence rates This finding merits further examination, ... values, occurred in monitoring sites located in the northwestern states, including Boise Idaho, Spokane Washington, and Helena Montana (30) However, 2001-2005 thyroid cancer incidence in these states...
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Báo cáo khoa học: Study of uptake of cell penetrating peptides and their cargoes in permeabilized wheat immature embryos pot
Ngày tải lên :
23/03/2014, 07:20
... of CPPs and their cargo [7,14,24] Interestingly, most investigations involving CPPs have been carried out in mammalian cell lines and there are only few reports on translocation of CPPs in plant ... proteins by arginine-rich intracellular delivery (AID) and Tat-protein transduction domain has been reported in onion and corn root tip cells [28,29] Cationic oligopeptides such as polyarginine ... for protein delivery in mammalian cell lines has also been shown to deliver GUS enzyme in the immature embryos M-Tat (with substitution of first arginine with an alanine in Tat basic domain) served...
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Báo cáo khoa học: Dictyostelium differentiation-inducing factor-1 induces glucose transporter 1 translocation and promotes glucose uptake in mammalian cells pdf
Ngày tải lên :
23/03/2014, 09:20
... actions of insulin and other insulinomimetic substances in adipocytes Thus, we examined and compared the effects of DIF-1 and insulin on glucose uptake in 3T3-L1 adipocytes As shown in Fig 9A, ... DMEM-HG containing 1.7 mm insulin After 48 h, insulin was withdrawn from the culture medium and cells were maintained in DMEM-HG until use Measurement of the glucose concentration in the incubation ... ⁄ Akt-independent pathway We finally examined the effects of DIF-1 and insulin on the translocation of GLUT1 and GLUT4 in 3T3L1 adipocytes (Fig 9B) As expected, insulin induced both GLUT1 and GLUT4...
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Báo cáo khoa học: A novel pathway for sequential transformation of 7-dehydrocholesterol and expression of the P450scc system in mammalian skin pptx
Ngày tải lên :
23/03/2014, 13:20
... 8.1 and 15.6 The first peak had m/z, retention time and UV spectra (inset in D) corresponding to the 7-DHP standard (inset in D and inset in C) The product was at the limits of detectability in ... m/z 385.3 and UV spectra are shown in the inset in (B) and in inset in (D) These results are in agreement with recent findings of Thiboutot et al [36] of 22R-hydroxycholesterol conversion into 17-hydroxypregnenolone ... detectability only in human epidermal melanocytes and in a single melanoma line (SKMEL-188; representative panel Fig 1A) The lower band detected in keratinocytes, squamous cell carcinoma and melanoma...
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Báo cáo khoa học: Role of phosphorylation in p53 acetylation and PAb421 epitope recognition in baculoviral and mammalian expressed proteins pdf
Ngày tải lên :
30/03/2014, 16:20
... major nuclear localization signal of p53 and its phosphorylation involves S and G2 ⁄ M cyclin-dependent kinases [21] Our results now indicate that in protein derived from BVEs, an intact serine ... DNA binding function of p53 Cell 71, 875–886 25 Takenaka I, Morin F, Seizinger BR & Kley N (1995) Regulation of the sequence-specific DNA-binding function of p53 by protein kinase C and protein ... aspects in common and are linked in the regulation and function of p53 [4,5,22] The interplay between phosphorylation and acetylation of p53 at residues K373 to K382 is very interesting These...
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Báo cáo khoa học: Nuclear import of mPER3 in Xenopus oocytes and HeLa cells requires complex formation with mPER1 pdf
Ngày tải lên :
30/03/2014, 20:20
... mPER2 and mPER3 (Fig 2A) However, nuclear import rates A B Fig mPER1 contains an additional NLS in its C-terminal domain (A) Mapping of NLS function in murine Per proteins Fragments corresponding ... proteins and their phosphorylation Results Positive correlation between phosphorylation and nuclear import of mPER proteins in Xenopus oocytes The different individual murine PER and CRY proteins ... slowly exported and there is no indication of protein degradation in the nucleus Thus, in summary, microinjection of individual isolated murine PER proteins reveals that mPER1 and mPER2 become...
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Báo cáo khoa học: In vivo cross-linking of nucleosomal histones catalyzed by nuclear transglutaminase in starfish sperm and its induction by egg jelly triggering the acrosome reaction pdf
Ngày tải lên :
31/03/2014, 07:20
... endogenous nuclear transglutaminase Occurrence of transglutaminase in sperm chromatin We next examined whether nuclear transglutaminase is present in the chromatin fraction by carrying out an ... transition in the chromatin structure occurs during spermatogenesis in sea urchins In mature sea urchin sperm, no protamines are present and the nucleosomal structure is retained [26] Chromatin of ... chromatin organization in the sperm of starfish Such cross-linking might be involved in chromatin compaction Starfish sperm histone H2B contains Gln43 and Gln91 in addition to Gln9 [5] Our finding,...
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nuclear transplantation and new frontiers
Ngày tải lên :
10/04/2014, 22:10
... oocyte nuclear proteins with nuclear reformation allowing reprogramming of the chromatin 32 NUCLEAR TRANSPLANTATION AND NEW FRONTIERS IN GENETIC MOLECULAR MEDICINE DEFINING THE LIMITS OF NUCLEAR ... difficult Even in mice, success in establishing and DEFINING THE LIMITS OF NUCLEAR REPROGRAMMING IN MAMMALS 33 maintaining totipotent ES cell lines has been largely limited to the 129 strain Selection ... IN GENETIC MOLECULAR MEDICINE nuclear reprogramming following a secondary nuclear transplantation, also referred to as “recloning.” Selection of the most well-developed embryos from initial nuclear...
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báo cáo hóa học:" Retinal pigment epithelial cells secrete neurotrophic factors and synthesize dopamine: possible contribution to therapeutic effects of RPE cell transplantation in Parkinson''''s disease" doc
Ngày tải lên :
18/06/2014, 15:20
... (400 mg/kg) and received brain injection on a stereotaxic frame (Myneurolab, USA) 6-OHDA (6 μg in μl) was dissolved in normal saline containing ascorbic acid (0.2 mg/ml), and injected into the right ... containing transplants and mounted to gelatin coated slides Adjacent sections were processed for hematoxylin-eosin (HE) stain and immunohistochemistry The sections were stained against cytokeratin ... (4°C) for 10 minutes Brains were removed, postfixed for hours in PFA and then cryoprotected for 24 hours in PBS with 30% sucrose Before frozen in -80°C, the brains were embedded in embedding medium...
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Báo cáo hóa học: "Expression of 14-3-3 protein isoforms in mouse oocytes, eggs and ovarian follicular development" pdf
Ngày tải lên :
21/06/2014, 19:20
... switching The proteins bind to a diverse set of target proteins and alter cellular function by binding to and causing conformational changes in target proteins or modifying target protein interactions ... metabolism and protein trafficking We are particularly interested in the role of 14-3-3 in meiosis in mammalian eggs and the role 14-3-3 proteins may play in ovarian function Therefore, we examined ... arrows indicate the primordial or primary follicles in (A and B) Note the weaker staining in mural granulosa cells in secondary follicles (C, green arrows) and the more intense staining in cells lining...
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Báo cáo sinh học: "Differences in the way a mammalian cell and yeast cells coordinate cell growth and cell-cycle progression" ppt
Ngày tải lên :
06/08/2014, 18:20
... remains linear for days but increases with increasing concentrations of serum In (a) the cells were cultured in 1% FCS, forskolin, and aphidicolin, while in (b) they were cultured in forskolin and ... Schwann cells were maintained in a proliferative state on poly-D-lysine- and laminin-coated culture dishes, in DMEM supplemented with 20 ng/ml GGF 2, M forskolin, 10 g/ml insulin, serum-free Schwann-cell-conditioned ... minutes in 0.4% Triton and 0.2% sodium dodecyl sulfate (SDS), in the presence of protease inhibitors (Boehringer Mannheim) and using a micro-BCA (bicinchoninic acid) assay with a bovine serum albumin...
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Báo cáo khoa học: "Autologous somatic cell nuclear transfer in pigs using recipient oocytes and donor cells from the same animal" pdf
Ngày tải lên :
07/08/2014, 20:23
... cultured for IVM to obtain as many oocytes as possible during autologous SCNT After washing twice in TLH-PVA and once in IVM medium, groups of 20-80 COCs were placed into individual wells of a ... Denmark) that contained 500 µl of IVM medium with IU/ml eCG (Intervet, Holland) and IU/ml hCG (Intervet, o Holland) COCs were cultured at 39 C in a humidified atmosphere of 5% CO2 in air After 22 ... containing µg/ml cytochalasin B After 40 h of maturation culture, denuded oocytes were incubated for 15 in a manipulation medium that contained µg/ml Hoechst 33342, washed twice in fresh medium, and...
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Báo cáo khoa học: "Post-thaw development of in vitro produced buffalo embryos cryopreserved by cytoskeletal stabilization and vitrification" pps
Ngày tải lên :
07/08/2014, 23:22
... solute transport systems Maintaining the integrity of the cytoarchitecture within an embryo during cryopreservation is, thus, of utmost importance In porcines, cyto-b-treated embryos display better ... day and blastocysts on day were selected for vitrification Microfilament stabilization and vitrification Embryos were incubated in a CO2 incubator for 45 in an embryo culture medium consisting ... 25% EG and 25% DMSO in base medium and 0.3 M sucrose for 45 sec During the second exposure step, the embryos were loaded into the middle part of 0.25 ml straws (IMV; 3∼4 embryos per straw) in a...
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Báo cáo khoa học: "Effects of irrigation and nitrogen fertiliser on the growth and nutrient relations of Prunus avium L and ’Colt’ (Prunus avium x Prunus pseudocerasus) in the nursery and after transplantation" doc
Ngày tải lên :
08/08/2014, 19:21
... INTRODUCTION This report investigates the interaction nitrogen fertiliser and irrigation on the growth and nutrient relations of cherry seedlings and cuttings in the liner nursery and the ... pseudoplatanus) seedlings grown in pots, and receiving a high rate of nitrogen in year and a low rate in year (after transplanting) were unable to sustain early leaf growth rates later in year 2, whereas ... digestion in concentrated H con4 SO taining 0.1 % Se as a catalyst and H Nitrogen O was determined on a Technicon Autoanalyzer using the indophol blue method and P was determined using the molybdenum...
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Báo cáo y học: "Effect of a small molecule inhibitor of nuclear factor-κB nuclear translocation in a murine model of arthritis and cultured human synovial cells" pps
Ngày tải lên :
09/08/2014, 07:20
... These include a proteasome inhibitor PS-341 [14], and IκB kinase inhibitors BMS-345541 [15] and SPC 839 [16], which improved clinical and pathologic findings in murine arthritis Another NF-κB inhibitor ... DHMEQ inhibits TNF-α-induced nuclear translocation of NF-κB, and does not inhibit phosphorylation and degradation of IκB, or a c-Jun N-terminal kinase (JNK) and a caspase-activating pathway in Jurkat ... The left hind paw of each mouse was dissected, formalin-fixed, decalcified, embedded in paraffin, and stained with hematoxylin and eosin Synovitis and bone destruction around the MTP joints of...
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Báo cáo y học: "The role of transposable elements in the evolution of non-mammalian vertebrates and invertebrates" doc
Ngày tải lên :
09/08/2014, 20:22
... Figure The fraction of introns containing TEs and their median lengths in non -mammalian and mammalian transcriptomes (a) The fraction of TE-containing introns within five non -mammalian genomes compared ... Transposable elements in Ciona intestinalis TE Exons in non-RefSeq alignments† Total Intronic TEs in introns within RefSeq TEs in introns of non-RefSeq Exons within RefSeq alignments* SINE 51,021 20,360 ... introns), C intestinalis (total of 34,328 introns), D melanogaster (total of 41,145 introns) and C elegans (total of 98,695 introns) for TE insertions to determine the percentage of TE-containing introns...
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