nuclear transplantation and new frontiers in genetic molecular medicine
gene therapy for liver disease
... intrathymic injections and oral administrations of adenoviral protein extracts or by neonatal administration of the virus in utero, thereby increasing long-term expression and allowing readministration ... encodes a single polyprotein of 3010 to 3033 amino acids in length Posttranslational processing results in the RNA binding nucleocapsid protein C, the envelope proteins E1 and E2, and the nonstructural ... and was intravenously injected into mice A single dose of liposomal-delivered plasmid induced the production of human AAT in mouse hepatocytes and resulted in measurable levels of human AAT in...
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Báo cáo sinh học: "AAV-mediated gene therapy for metabolic diseases: dosage and reapplication studies in the molybdenum cofactor deficiency model" pps
... Donahue BA, Lin HF, Stafford DW, Patel S, Thompson AR, Nichols T, Read MS, Bellinger DA, Brinkhous KM, Kay MA: Correction of hemophilia B in canine and murine models using recombinant adeno-associated ... received a single injection containing × 108 tu (n = 12) around weeks [9-14] This difference was mainly attributed to prior infection of the human patients with natural AAVs in combination with ... above At day 40, the two animals obtained a single intravenous tail vein injection of 65 μl containing × 109 tu AAV-MOCS1 The successful transduction of this first injection was confirmed by the prolonged...
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Báo cáo khoa học: Active-site-specific chaperone therapy for Fabry disease Yin and Yang of enzyme inhibitors pptx
... domain (amino acid residues 32–330), and a C-terminal domain (residues 331– 429) containing eight antiparallel b strands on two sheets in a b sandwich (Fig 1A) [28] The first domain contains the ... (red) terminus Domain contains the active-site at the center of the b strands in the (b ⁄ a)8 barrel, whereas domain contains antiparallel b strands The galactose ligand is shown in yellow and red ... protein folding in favor of proper folding, thereby increasing intracellular enzyme activity (Fig 3) Retrospectively, these enzyme inhibitors could be useful tools for probing and assessing the...
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gene therapy for hematological disorders
... chromatin and integrate into the genome (right frame) a number of modifications in the genetic sequences included in packaging cell lines has greatly decreased the risk of recombination events, and ... transfer into baboon marrow repopulating cells using recombinant human fibronectin fragment CH-296 in combination with interleukin-6, stem cell factor, FLT-3 ligand, and megakaryocyte growth and development ... clinical studies, thereby tracking the same gene-modified cell population in both patients and immunodeficient mice Clinical Genetic Marking Studies Genetic marking of cells with an integrating...
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gene therapy in cardiovascular disease
... generating increased collateral flow But documented clinical success in reducing angina scores and improving myocardial perfusion in otherwise untreatable patients has been observed In a porcine ... (A and B) and antisense oligonucleotide (against c and kinase/PCNA)-treated vein grafts (C and D) in hypercholesterolernic rabbits, weeks after surgery (¥7O) See color insert Sections were stained ... color insert (A) Hoechst 33,342 nuclear chromatin staining of vein graft in cross section, illustrating location of nuclei within the graft wall (100¥) (B) Same section of saphenous vein viewed...
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gene therapy for hiv infection
... HIV infection in a clinical setting has been disappointing The intravenous infusion of recombinant sCD4 protein in HIV-infected patients failed to show efficacy in phase I clinical trials In contrast ... through its interaction with the ER-specific BiP protein The BiP protein binds incompletely folded immunoglobulins and may facilitate the folding and/ or oligomerization of these proteins Intrabodies ... (ER), and rearranged heavy- and lightchain variable regions that are connected by a flexible interchain linker Since the single-chain antibody cannot be secreted, it is efficiently retained within...
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Báo cáo y học: " Chitosan Interferon-c Nanogene Therapy for Lung Disease: Modulation of T-Cell and Dendritic Cell Immune Responses" pps
... transforming growth factor b and procollagen I and III and decreases fibrosis in a mouse model of bleomycin-induced lung injury.35 Exogenously supplied cytokines, such as IFN-c, IL-12, and IL-18, ... above, and an amount containing 10 mg of pEGFP was administered intranasally to mice After 1, 3, and days, the mice were euthanized and the lungs were removed and fixed in buffered formalin Whole ... side-scatter/forward-scatter set for lymphocytes and gating for CD8+ tetramer Unstained cells were run as a control, and non-viable cells were distinguished by staining with 7-amino-actinomycin D Tetramers use a mutated...
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Báo cáo y học: " Cell therapy for autoimmune diseases" ppt
... In SSc, durable skin softening in patients with established generalized skin thickening has been observed in two-thirds of patients transplanted, defying conventional wisdom that fibrotic skin ... to normalize and returned to pretreatment levels before relapse In SLE patients, antinuclear antibody and antidouble-stranded DNA antibodies disappeared in many patients after HSCT and returned ... peripheral tolerance and they modulate susceptibility to autoimmune disease [41] and tumour immunity [43], as well as playing a role in the induction of transplantation tolerance [44] and in the regulation...
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báo cáo khoa học: "Reconsidering low-dose aspirin therapy for cardiovascular disease: a study protocol for physician and patient behavioral change" pot
... aspirin therapy for primary and secondary cardiovascular disease prevention in six General Internal Medicine and Family Medicine clinics within the University of Colorado Hospital system using ... collected using the University of Colorado Hospital’s Allscripts database and the QED Clinical, Inc (DBA-CINA) Clinical Data Repository maintained by the Department of Family Medicine Our post-intervention ... surrounding effective methods for discontinuation of current practices Effecting a change in aspirin usage in one or more groups will result in a better understanding of the approaches and efforts...
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Báo cáo y học: " Taking stock of gene therapy for cystic fibrosis" pdf
... UTP-binding P2Y2 receptor Novel viruses are increasingly being investigated, and cationic liposomes and polymers are increasingly being coupled to peptides, sugars and viral particles to try to increase ... expression of the protein and positive changes in clinical endpoints, including circulating levels of factor IX and the frequency of infusion of factor IX protein The questions that remain to be answered ... regions lined by a pseudostratified columnar epithelium and containing numerous submucosal glands, and the small bronchiolar regions lined by a simple columnar epithelium devoid of glands A central...
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Báo cáo sinh học: "Careful adjustment of Epo non-viral gene therapy for β-thalassemic anaemia treatment" pot
... significant hematocrit increase which was maintained between 35.4% and 38.7% during 10 months (fig 2-A and Page of (page number not for citation purposes) Genetic Vaccines and Therapy 2008, 6:10 ... by intra-peritoneal injection of 250 μl of a ketamine-xylazine solution (respectively 8.66 mg/ml and 0.31 mg/ml in 150 mM NaCl) Left rear legs were shaved and the Epo-plasmid solution was injected ... different doses and administration schedules of an Epo encoding plasmid in anaemia treatment of β-thalassemic mice Considering electrotransfer advantages in terms of safety, efficiency and cost, we...
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The natural history of non-alcoholic fatty liver disease in children: a follow-up study for up to 20 years pptx
... requiring liver transplantation in paediatric NAFLD remains uncertain, and further studies in this area are needed It is also intriguing that both cases undergoing liver transplantation in our ... and prothrombin time were essentially within the normal range in all patients Positive autoantibodies in low titres were found in 20% of patients including ANA in 15.4%, and SMA in 10% of patients ... oxidative stress and it could be released by several conditions inducing cellular stress and insulin resistance; both insulin resistance and oxidative stress are key components in the development...
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Gene Transfer Approaches for Gynecological Diseases pot
... Ad5/3-D24 Main result In vitro in vivo In vitro in vivo In vitro in vivo In vitro in vivo In vitro in vivo and and and and and Killing of ovarian cancer cells, expression of soluble marker peptide Intercellular ... [61] [106] [107] [108] In vitro In vitro Growth inhibition of E6-positive cells Cell killing of HPV-positive cells [69] [66] In vitro and in vivo In vitro and in vivo In vitro Inhibition of cervical ... involves genetic modifications of the viral capsid Enhanced infectivity of ovarian cancer cells has been demonstrated by incorporating an integrin-binding RGD-4C motif in the HI loop of the fiber MOLECULAR...
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Obesity and Air Pollution: Global Risk Factors for Pediatric Non-alco- holic Fatty Liver Disease potx
... well as alterations in insulin/insulin-like growth factor and insulin resistance, which contribute to fatty liver, an escalating trend in the incidence of pediatric NAFLD and its related complications ... obesity and co-morbidities in Europe Part The increase in the prevalence of child obesity in Europe is itself increasing Int J Pediatr Obes 2006;1(1):26-32 Haas GM, Liepold E, Schwandt P Predicting ... Papademetris X, et al Alanine aminotransferase levels and fatty liver in childhood obesity: associations with insulin resistance, adiponectin, and visceral fat J Clin Endocrinol Metab 2006;91(11):4287-94...
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nonviral vectors for gene therapy, methods and protocols
... removing the BglII and Cationic -Helical Peptides 13 HindIII insert of the plasmid PGV-C (Toyo Ink), and ligating with the BglII and HindIII fragment from the pRc/CMV (Invitrogen), which contains ... combination of mobile phase and columns must be chosen, because polyelectrolytes including HA and PLL are liable to interact with the column packings, leading to delay in elution volume In such ... Methods in Molecular Medicine, vol 65: Nonviral Vectors for Gene Therapy Edited by: M A Findeis © Humana Press Inc., Totowa, NJ 35 36 Bos, Crommelin, and Hennink Fig Poly(2-[dimethylamino]ethyl...
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viral vectors for gene therapy
... procedures, and should prove useful for both experienced investigators and newcomers in the field, including those beginning graduate study or undergoing postdoctoral training The “Notes” section contained ... France KRISTIN BAER • Department of Molecular Medicine & Pathology, Faculty of Medical and Health Sciences, The University of Auckland, Auckland, New Zealand CATHERINE BARJOT • UMR INRA, Nantes ... synthesis and encode proteins such as DNA binding proteins, polymerases, and thymidine kinase Viral DNA synthesis occurs by a rolling circle mechanism, forming head-to-tail concatemers of UL and US...
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Báo cáo hóa học: "Anti-viral state segregates two molecular phenotypes of pancreatic adenocarcinoma: potential relevance for adenoviral gene therapy" pdf
... Riley J, Rosenberg S, Parkhurst M: Comparison of common gammachain cytokines, interleukin-2, interleukin-7, and interleukin-15 for the in vitro generation of human tumor-reactive T lymphocytes for ... two independent taxonomies of cancer cells and not to the host’s reaction to the cancer as it is was observed in xenografts growing in immune deficient animals and in in vitro cultured cell lines; ... according to the coordinate expression of an inflammatory signature including cytokines, chemokines and angiogenic factors [16,31] The expression of these Monsurrò et al Journal of Translational Medicine...
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Báo cáo hóa học: " Abstract Despite new additions to the standard of care therapy for high grade primary malignant brain tumors, the prognosis for patients with this disease is still poor." ppt
... lysate administered in combination with chemotherapeutic agents [23,24] Another variation of the DC vaccine approach is being tested at Cedars-Sinai in Los Angeles (NCT00576641) and is enrolling recurrent ... cells and GM-CSF (TVAX) Later, autologous T cells are harvested and expanded ex vivo, and then administered intravenously Pilot clinical trials showed promising results with this approach to expand ... statistical oversight and immunologic endpoint integration needed and encourage cooperation between the small cohorts of investigators working in the immune therapy arena By doing so, integration of...
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Báo cáo sinh học: "Comparisons of three polyethyleneimine-derived nanoparticles as a gene therapy delivery system for renal cell carcinoma" doc
... units/ml of penicillin and 100 units/ml of streptomycin All cells were routinely maintained at 37°C in humidified air containing 5% CO2 Page of 10 Reagents Dimethyl sulfoxide (DMSO) and 3-(4, 5-dimethylthiazol2-yl)-2, ... solved in 0.1 ml PBS and μg pVITRO2-VHL plasmids, and each mouse in the VHL-treated group was injected polyplexes by tail vein for 10 times at 2-days intervals The mice in the pVITRO2 and PBS ... FA-PCFC-isophorone diidocyanate-PEI (FA-PEAs) and heparin-PEI (HPEI) in vitro and in gene therapy carrying with Von Hippel-Lindau (VHL) on mice RCC model These new attempts provide potential methods...
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Báo cáo sinh học: "Safety and feasibility of third-party multipotent adult progenitor cells for immunomodulation therapy after liver transplantation–a phase I study (MISOT-I)" doc
... screening visits or clinical investigations in addition to standard pre-transplant work-up Standardof-care examinations for patients on the Liver Transplant Waiting List will be performed, including ... started on postoperative day and tapered by month after liver transplantation, MAPC infusions will be administered into the portal vein during transplantation and later intravenously on day analysis ... procedures to examine the liver and lung after intraportal and intravenous injection, respectively Toxicity related to intraportal infusion will be assessed by Doppler ultrasound determining the maximum...
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Tìm thêm: khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo gắn với các giáo trình cụ thể tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng 9 tr 25