nuclear magnetic resonance structural studies of the fxyd family membrane proteins in lipid bilayers
Báo cáo khoa học: Structural studies of the capsular polysaccharide and lipopolysaccharide O-antigen of Aeromonas salmonicida strain 80204-1 produced under in vitro and in vivo growth conditions docx
Ngày tải lên :
23/03/2014, 13:20
... determined following the hydrolysis of the carboxyl-reduced CPS Presence of L-alanine was confirmed by amino acid analysis For this, 0.6 mg of CPS was hydrolysed in 5.7 M hydrochloric acid containing ... used in most of 1D analogues of 2D NMR experiments The 1D 1H-NMR spectrum of CPS showed three resonances in the low-field region (5.3–4.4 p.p.m.) in the relative Ó FEBS 2004 ratio of : : 3, of which ... during the 72-h in vivo culture period In addition, sugars detected in the inoculum TSB culture were also present Based on sugar ratios of 3-amino-3-deoxy-D-quinovose and L-rhamnose, 10% of the...
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Báo cáo khoa học: Fluorescence studies of the replication initiator protein RepA in complex with operator and iteron sequences and free in solution pdf
Ngày tải lên :
07/03/2014, 04:20
... titration In the ts, the protein concentration was left free, to serve as an indicator of stoichiometry In the case of 1IR, the t resulted in a binding stoichiometry of : 1, i.e dimer binding The reactant ... quenching However, judging from the binding stoichiometry together with the shape of the binding curves (Fig 3), it is safe to conclude that the quenching does not affect the RepADNA binding equilibria, ... rearrangement of the relative orientation of the two domains [7,9] The conformational change upon iteron binding may expose a recognition site for proteinprotein interaction, enabling coupling of recently...
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Báo cáo khoa học: A structural overview of the PDI family of proteins docx
Ngày tải lên :
15/03/2014, 23:20
... determination of the structure of the ERp72 catalytic domains allowed a glimpse into the effects of local rearrangements of the N-terminal part of helix a2 on positioning of the conserved arginine ... suggesting that the C-terminal cap of the substrate binding domains contributes to the specificity of ERp44 How the action of the C-terminal tail of ERp44 is regulated in cells is an intriguing question ... [46] The recently determined ERp57–tapasin structure provided the first structural insights into protein binding by the catalytic domains of a mammalian PDI [13] In the structure, the a domain of...
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Báo cáo Y học: Structural analysis of the lipopolysaccharide from nontypeable Haemophilus influenzae strain 1003 potx
Ngày tải lên :
31/03/2014, 21:21
... significantly increasing amounts of terminal Glc and 2-substituted Hep, thereby indicating phosphorylation of these residues in the native material The absence of 3-substituted Gal as compared with the ... ACKNOWLEDGEMENTS The authors would like to thank sincerely the members of the Finnish Otitis Media Study Group at the National Public Health Institute in Finland for the provision of NTHi strains from the ... 5.6 and 5.0 Three of these signals were anomeric resonances of the heptose residues (HepI– HepIII) in the inner-core region The Hep ring systems were identified on the basis of the observed small...
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báo cáo hóa học: " A psychometric evaluation of the PedsQL™ Family Impact Module in parents of children with sickle cell disease" pptx
Ngày tải lên :
18/06/2014, 18:20
... comprised of the average of the items in the emotional, social, and school functioning scales The physical health summary score is comprised of the average of items in the physical functioning scale ... To determine the association between the parent's HRQL and family functioning with the proxy-report of their child's HRQL, we examined the correlation between the scores on the PedsQL™ Family Impact ... A number of limitations should be considered in evaluating these study results The parents were involved in the study during a routine clinic visit and so the reported scores are not in direct...
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Tài liệu Báo cáo khoa học: Structural and functional studies of the human phosphoribosyltransferase domain containing protein 1 docx
Ngày tải lên :
15/02/2014, 01:20
... and N7 (Fig 1C) The 2¢OH of the ribose is hydrogen bonded to the main chain and the side chain of Asp142 (Fig 1C) Both hydroxyl groups of the ribose are involved in coordinating a putative Ca2+ ... for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Anal Biochem 72, 248–254 Page R & Stevens RC (2004) Crystallization data mining in structural ... PRPP binds first, inducing a conformation change, then allowing the purine base to bind in accordance with the mechanism of human HPRT [19,25] This conclusion can be made based on the lack of thermal...
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Báo cáo Y học: Structural studies on the core and the O-polysaccharide repeating unit of Pseudomonas aeruginosa immunotype 1 lipopolysaccharide pot
Ngày tải lên :
24/03/2014, 03:21
... aeruginosa LPS [4,5] As in the LPS of P aeruginosa O5 [4], in the LPS of immunotype (serogroup O6) the terminal fi 3-linked Rha residue of the core oligosaccharide is the site of the attachment of ... by the signal intensities of the methyl groups of Rha and QuiN, in P aeruginosa immunotype glycoforms and are present in almost equal amounts The remainder of the degraded first repeating unit of ... reducing end of the biological repeating unit, has the b configuration when linking the O-polysaccharide to the core but the a configuration when connecting the repeating units to each other in the O-polysaccharide...
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Báo cáo y học: "Diffusion-weighted magnetic resonance imaging for predicting the clinical outcome of comatose survivors after cardiac arrest: a cohort study" pptx
Ngày tải lên :
13/08/2014, 20:21
... magnetic resonance imaging (MRI), is useful in assessing the extent of structural brain injury Yet, evaluating hypoxic ischemic brain injury with CT or conventional MRI often underestimates the ... assessment of the severity of brain damage by means of measuring the apparent diffusion coefficient (ADC) [9-12] The patterns and extent of brain injury seen in DWI are associated with clinical outcomes ... nuclei injuries The ADC values were only obtained from 22 patients who were examined using the GE Signa Excite due to the use of two kinds of MR scanners On the workstation, the ADC value of each...
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Báo cáo y học: "Characterization of N200 and P300: Selected Studies of the Event-Related Potential Salil H. Patel 1 and Pierre N. Azzam 2"
Ngày tải lên :
02/11/2012, 11:08
... effect on the latency and amplitude of the evoked MMN, the elicited N2b was found to continuously increase in latency and decrease in amplitude with increasing age These findings suggest the constancy ... underlying state of CNS dis-inhibition involved in the pathophysiology of the condition [93] Genetic linkage studies involving families with a history of alcoholism show involvement of chromosomes ... of the sensory auditory memory trace with increasing age [19] The same phenomenon occurs in the very young, indicating the necessity of maturational changes for the complete efficiency of the...
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Tài liệu SURVEY OF CASE STUDIES OF THE USE OF KNOWLEDGE MANAGEMENT IN SOFTWARE ENGINEERING docx
Ngày tải lên :
16/01/2014, 16:33
... ask whether the introduction of a knowledge management system improves the quality of software, only one of the studies, from the Software Engineering Laboratory gives a clear answer Asking if ... according to the subject of study; in software engineering it can be either a process to produce software or a software product In an article on research methods in software engineering [30] we ... Sommerville, Software Engineering, Addison-Wesley, 1996 A Bryant, Its Engineering Jim but not as we know it Software Engineering Solution to the software crisis, or part of the problem?, in Proc Int...
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Tài liệu Báo cáo khoa học: Mechanism of dihydroneopterin aldolase NMR, equilibrium and transient kinetic studies of the Staphylococcus aureus and Escherichia coli enzymes docx
Ngày tải lên :
19/02/2014, 00:20
... set of control data was obtained in the absence of the protein The data set obtained in the absence of the protein was then subtracted from the corresponding data set obtained in the presence of ... DHNA inhibitors The binding of the inhibitors to the enzymes cause a decrease in their fluorescence intensities The increasing fluorescence intensities in Fig 5A were obtained by subtracting the ... performed in the absence of the ligand The control data set obtained in the absence of the ligand was subtracted from the corresponding data set obtained in the presence of the ligand The Kd values...
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Tài liệu Báo cáo Y học: Proteolysis of bovine b-lactoglobulin during thermal treatment in subdenaturing conditions highlights some structural features of the temperature-modified protein and yields fragments with low immunoreactivity pptx
Ngày tải lên :
21/02/2014, 15:20
... those of monomers obtained at low pH [35] or under pressure [10] Arg148 is not the only buried arginine in the structure of BLG The whole side chain of Arg40 (the other main point of trypsin action) ... virtually insensible to hydrolysis by either trypsin or chymotrypsin in the absence of a thermal unfolding step, as less than 10% of the native protein was degraded by either enzyme in 20 at 37 ... treatment, hydrolysis of BLG was extensive, in spite of significant inactivation of the enzymes This indicates that the transient conformers originating in the course of thermal treatment had...
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Tài liệu Báo cáo Y học: Molecular modeling of the dimeric structure of human lipoprotein lipase and functional studies of the carboxyl-terminal domain docx
Ngày tải lên :
21/02/2014, 15:20
... the catalytic site in the N-terminal domain of one subunit and the substrate recognition site in the C-terminal domain of the other face each other, thereby bringing together the substrate-binding ... The cluster of basic residues in the C-terminal domain may constitute heparin binding-site residues that coordinate with heparin-binding residues in the N-terminal domain The cluster of hydrophobic ... demonstrated in a molecular modeling experiment that the C-terminal domain of apoCII interacts with the interface of the N- and C-terminal domains of LPL and part of the lid surface [32] Because the functional...
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Tài liệu Euphorion Being Studies of the Antique and the Mediaeval in the Renaissance - Vol. II pot
Ngày tải lên :
21/02/2014, 21:20
... daughters -in- law, forming a hierarchy attending to the business of factory or counting-house under the orders of the father of the family, and to the economy of the house-under the superintendence of the ... noise of the streets, the looks of the crowd, the great minster rising half-finished in the midst of the town by the Rhine, the cries and noise and chipping of the masons; unconscious of all ... to the north, into the widening torrent valleys of the Modenese Apennines to the south, the Marquisate of Este stretches up into feudalism, into chivalry, into the imaginative kingdom of the...
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Tài liệu Báo cáo Y học: Structural determinants of the half-life and cleavage site preference in the autolytic inactivation of chymotrypsin pdf
Ngày tải lên :
22/02/2014, 07:20
... than in the interdomain loop (at Phe114) The lack of temporary accumulation of fragment III during the degradation of the Phe114 !Ile interdomain loop mutant clearly indicates that the cleavage of ... A.G (1982) The activity of pepsin, chymotrypsin and trypsin during 24 h periods in the small intestine of growing pigs Br J Nutr 48, 147–159 Hofstee, B.H.J (1965) The rate of chymotrypsin autolysis ... the enzyme [8].) Two further sets of experiments were performed to test the role of cleavages in the interdomain and autolysis loops in the inactivation of chymotrypsin At first, D-chymotrypsin...
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Tài liệu Báo cáo Y học: Purification, characterization, immunolocalization and structural analysis of the abundant cytoplasmic b-amylase from Calystegia sepium (hedge bindweed) rhizomes ppt
Ngày tải lên :
22/02/2014, 07:20
... residue preceding this methionine is shown in lower case The sequence corresponding to the N-terminal sequence of the protein is underlined The arrowheads indicate the positions of the intron sequences ... for the b-amylase from C sepium using the X-ray coordinates of the soybean b-amylase (Fig 4) According to the Ramachandran plot of this model the f and c angles of most of the residues are in the ... from the removal of the N-terminal methionine, no co- or post-translational processing According to the results of previously reported molecular and structural studies the processing of the b-amylases...
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Báo cáo khoa học: Molecular basis of perinatal hypophosphatasia with tissue-nonspecific alkaline phosphatase bearing a conservative replacement of valine by alanine at position 406 Structural importance of the crown domain potx
Ngày tải lên :
07/03/2014, 05:20
... further supporting that the folding and assembly process and subsequent intracellular trafficking of TNSALP (V406A) are largely normal in the stable cell line Kinetics of the soluble form of TNSALP ... that was < 10% of that of the wildtype enzyme (Table 1), indicating that the conversion of valine to alanine at position 406 in the crown domain compromises the catalytic function of TNSALP Protease ... form of the wild-type enzyme, indicating that the replacement of valine with alanine at position 406 in the crown domain somehow strongly affects the catalytic efficiency of TNSALP Considering...
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Báo cáo khoa học: Structural mobility of the monomeric C-terminal domain of the HIV-1 capsid protein pptx
Ngày tải lên :
07/03/2014, 06:20
... from a thermodynamic point of view, for the binding of the monomeric species of CAC? We have previously discussed the variation in the free energy of binding as a function of the changes in buried ... promote other interactions in the presence of an external agent (lipids, peptides, other regions of the Gag protein, or even other proteins) In the first three examples, the second helix (as in CACW40A) ... vectors are independent of the overall rotational movement of the molecule) Furthermore, the internal motions of each bond vector are independent of each other, but the rotational diffusion of the molecule...
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Báo cáo khoa học: Purification and structural analysis of the novel glycoprotein allergen Cyn d 24, a pathogenesis-related protein PR-1, from Bermuda grass pollen pot
Ngày tải lên :
07/03/2014, 12:20
... immunoblotting techniques, our recent studies have indicated that BGP contains about 230 proteins including isoforms ⁄ degraded protein products and around 65 of these are IgE-binding proteins [19] ... staining was performed using a Glycoprotein staining kit (Pierce, Rock, IL, USA) according to the manufacturer’s instruction Lectin-binding assays The ability of Cyn d 24 to bind various lectins ... alignment of Cyn d 24 (A) Nucleotide and deduced amino acid sequences of Cyn d 24 The numbers on the right of the figure indicate the positions of the nucleotide sequence The numbers on the left of the...
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Báo cáo khóa học: Structural properties of the protein SV-IV potx
Ngày tải lên :
07/03/2014, 14:20
... protein is organized as a-helix in the whole protein, and the 1–70 region is poorly structured SDS increases the a-helix content of proteins revealing the helical potential The a-helix content of ... forms of the protein These contained from three to eight acetyl groups (the maximum expected was 10, considering nine lysine residues and the N-terminal amino group), the most abundant ranging ... occurring in going from the monomeric to the trimeric form Predictive methods have been applied to obtain a theoretical model of the structural organization of SV-IV protein The amino-acid sequence...
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