normalization differential gene expression and clustering
Báo cáo y học: "Mining for coexpression across hundreds of datasets using novel rank aggregation and visualization methods" pptx
Ngày tải lên :
09/08/2014, 20:21
... exceeding the threshold and the number of genes in the result of the query We achieved strongest coexpression patterns between the query genes and the resulting genes when using a standard deviation ... expression profiles and has become a standard method of measuring coexpression [25] Another useful measure is the anti-correlation distance that detects inverse expression patterns, such as genes ... retrieves genes with high expression similarity and functional relevance to the query gene (Figure 1) Output The principal output of MEM is a ranked list of genes that are coexpressed with the query gene...
- 11
- 401
- 0
scientific american - 1993 09 - special issue - life, death, and the immune system
Ngày tải lên :
12/05/2014, 14:50
... from the size of these minigene families: there are more than 100 kinds of V (variable) genes, 12 D (diversity) genes and four J (joining) genes The C, or constant, genes vary in ways that aÝect ... partial genes: a V gene segment, a J (for joining) segment and sometimes also a D (for diversity) gene segment The unique variability of V domains results from gene rearrangement, which generates ... random combinations of specialized gene segments The alpha and beta chains of T cells, for example, are created by a random mixture of gene segments known as Valpha, J-alpha, V-beta, D-beta and...
- 119
- 910
- 1
Báo cáo Y học: A new siglec family member, siglec-10, is expressed in cells of the immune system and has signaling properties similar to CD33 docx
Ngày tải lên :
24/03/2014, 04:21
... vector, Gibco/BRL, Grand Island, NY, USA) for several splice variants were obtained from Incyte and sequenced Sequencing and alignment Individual clone colonies were cultured and DNA was isolated ... II and Nco I The amplified fragment was cloned into pd19 by digesting the fragment with HindIII and Bgl II and the plasmid with HindIII and Bam HI The fragment was ligated into the plasmid and ... were generated at positions 597, 641, 667, and 691 To construct the GST fusions of the tandem SH2 domains of each SHP-1 and SHP-2, the sequences corresponding to amino acids 2–232 of each SHP-1 and...
- 14
- 540
- 0
Báo cáo sinh học: "Parasite immunomodulation and polymorphisms of the immune system" ppsx
Ngày tải lên :
06/08/2014, 19:20
... self-antigens and allergens [1,2] Keeping a balance Although the hygiene hypothesis is couched in very general terms, there is strong evidence that specific geneenvironment (and more specifically, gene- parasite) ... adaptive immune system genes (in particular, at Treg-associated loci) suggests that there is no certain genetic optimum and that, in an environment with diverse pathogens demanding conflicting response ... species) and host genetic diversity that has recently been documented in a report on cytokine gene polymorphism by Fumagalli et al [3] In an analysis of nearly 100 human interleukin genes, they...
- 4
- 386
- 1
Báo cáo y học: "Regulating the immune system: the induction of regulatory T cells in the periphery Jane H Buckner1 and Steven F Ziegler2" pot
Ngày tải lên :
09/08/2014, 01:24
... to be generated in vitro and in vivo in systems using both self-antigens and foreign antigens The present review will summarize the recent findings on the development of acquired Tregs, and on ... liver, the lung, and the intestine, and they appear to function via IL10 secretion [32,38–40] Wakkach and colleagues [41] recently identified a subset of dendritic cells in the spleen and lymph node ... originate in the thymus, and are expanded in the periphery, or whether they have been generated in the periphery The possibility exists that, due to differences between mouse and man in life expectancy,...
- 8
- 478
- 0
Báo cáo y học: " Mimicking microbial ‘education’ of the immune system: a strategy to revert the epidemic trend of atopy and allergic asthma?" ppt
Ngày tải lên :
12/08/2014, 18:20
... ‘unhygienic’ areas inhale and ingest a different kind, variety and amount of bacteria compared with children of farmers and anthroposophic families, who have access to natural soil and eat only biologically ... that facilitate or protect against allergy We must still learn whether and what kind of hygienic measures cause atopy, and generic antihygienic procedures would obviously facilitate the spread ... diversified set of bacteria and if they eat sufficiently contaminated food It would be interesting to determine the magnitude of exposure to orofaecal and food-borne infections [22], and to saprophytic...
- 4
- 335
- 0
Báo cáo y học: " Network, degeneracy and bow tie. Integrating paradigms and architectures to grasp the complexity of the immune system" pps
Ngày tải lên :
13/08/2014, 16:20
... global principles, and are organized according to general architectures and structures that work in a strictly and deeply intertwined manner, such as those of network, degeneracy and bow tie These ... high number of different inputs and, at the same Figure Degeneracy, pluripotentiality and bow tie The concept of bow tie integrates the concepts of degeneracy and pluripotentiality: figuratively, ... such as that of network, and that other layers of architectural complexity are present and should be identified, conceptualized and integrated The principle of degeneracy Degeneracy is a most prominent...
- 16
- 255
- 0
Báo cáo y học: "Common angiotensin receptor blockers may directly modulate the immune system via VDR, PPAR and CCR2b" ppt
Ngày tải lên :
13/08/2014, 23:20
... 11 Candesartan docked into the VDR ligand binding pocket Candesartan docked into the VDR ligand binding pocket Note: The core structure of the hydrogen-bonded residues is expanded to a 'ball -and- stick' ... arately and superimposed VDR-docked configurations for 1,25-D and Telmisartan, sepVDR-docked configurations for 1,25-D and Telmisartan, separately and superimposed Note: Models depicted as "thick" and ... Theoretical Biology and Medical Modelling 2006, 3:1 Figure docked configurations 1,25-D and TX522 with superimposed X-ray and VDR1,25-D and TX522 with superimposed X-ray and VDR-docked configurations...
- 33
- 246
- 0
Báo cáo y học: "Tumor microenvironments, the immune system and cancer survival" ppt
Ngày tải lên :
14/08/2014, 14:21
... concept and correlating gene- expression signatures with cells of origin and patient survival [12,25-27] The recent study [12] used whole-genome arrays, multiple clustering algorithms and knowledge ... chain genes) and a group called ‘BCR/proliferation’ (which showed elevated expression of genes encoding cell-cycle regulators, DNA-repair genes, the B-cell receptor signaling cascade, and B-cell ... only their gene- expression patterns, resulting in the identification of ‘diseases within diseases’, different subtypes of known cancers that differ in both gene expression patterns and prognosis...
- 4
- 235
- 0
Báo cáo y học: "Gene regulation and signal transduction in the immune system" pptx
Ngày tải lên :
14/08/2014, 20:22
... of gene regulation by cohesin is vertebrate specific Epigenetic regulation of inflammatory gene expression Two talks addressed the role of epigenetic mechanisms in regulation of inflammatory gene ... promoting B-cell development by restraining the expression of pro-myeloid factors (such as Gfi1), and acting directly to induce the recombinase (Rag) genes and thereby recombination at the immunoglobulin ... regulation of gene expression A plethora of studies in the past few years, and several talks at this meeting, mapped histone modifications genome-wide Keji Zhao (National Heart, Lung, and Blood Institute,...
- 4
- 268
- 0
Money, banking and the financial system (1st edition) r glenn hubbard, anthony patrick o''''brien prentice hall (2011)
Ngày tải lên :
18/08/2013, 19:46
... Rates and the Demand and Supply for Bonds 94 A Demand and Supply Graph of the Bond Market 94 Explaining Changes in Equilibrium Interest Rates 96 Factors That Shift the Demand ... 235 8.4 A Demand and Supply Model of Short-Run Movements in Exchange Rates 236 A Demand and Supply Model of Exchange Rates 236 Shifts in the Demand and Supply for Foreign ... each student’s performance and time spent on the Tests and Study Plan and generates reports by student or chapter A more detailed walk-through of the student benefits and features of MyEconLab...
- 644
- 1.3K
- 7
Paradoxical roles of the immune system during cancer development docx
Ngày tải lên :
15/03/2014, 01:20
... innate leukocytes by expression of somatically generated, diverse antigen-specific receptors, which are formed as a consequence of random gene rearrangements and allow a flexible and broader repertoire ... regulate tissue homeostasis and disease pathogenesis through pleiotropic biological effects, including remodelling of soluble and insoluble ECM components and cell–cell and cell–matrix adhesion ... prostaglandin biosynthesis, the expression of which is upregulated in inflamed and neoplastic tissues17 In several human epithelial cancers, expression of COX2 correlates with poor prognosis, and...
- 17
- 527
- 0
American Public Service: Radical Reform and the Merit System pot
Ngày tải lên :
22/03/2014, 19:20
... 129 Handbook of Globalization, Governance, and Public Administration, Ali Farazmand and Jack Pinkowski 130 Handbook of Globalization and the Environment, edited by Khi V Thai, Dianne Rahm and ... C Hyde 93 Handbook of Crisis and Emergency Management, edited by Ali Farazmand 94 Handbook of Comparative and Development Public Administration: Second Edition, edited by Ali Farazmand 95 Financial ... Rosenbloom and Richard D Schwartz 55 Handbook of Bureaucracy, edited by Ali Farazmand 56 Handbook of Public Sector Labor Relations, edited by Jack Rabin, Thomas Vocino, W Bartley Hildreth, and Gerald...
- 298
- 304
- 0
Generations Model and the Pension System
Ngày tải lên :
09/04/2014, 19:20
... overlapping generations model Generations Model and the Pension System c1,t and c2,t+1 are respectively her consumption in period t (when she is in the first period of life, and thus young) and her ... overlapping generations model Generations Model and the Pension System The overlapping generations model The households Individuals live for two periods In the beginning of every period, a new generation ... other generations or Download free eBooks at bookboon.com 16 Shifting from a pay as-you-go to a fully funded system Generations Model and the Pension System You’re full of energy and ideas And...
- 19
- 294
- 0
regional business cycle and real estate cycle analysis and the role of federal governments in regional stability
Ngày tải lên :
03/06/2014, 02:10
... enjoyable, and memorable Dianne (APO of the GC Economic department); Terissa, Maria, and Irina (Cafetaria Staffs); Allison, and Sylvia (Secretaries at Baruch College); and Mr Dauglas Ewing and other ... (2.1) (2.2) First, Beveridge and Nelson (1981), Nelson and Ploser (1982), and Cambell and Mankiw (1987) assume the stationarity of the rst log difference of the real GDP and emphasize on the usage ... I must thank Ben and Nicklina for going over my dissertation and proof reading it I would also like to thank my family members, all my cousins, aunts, uncles and in laws to stand by me Specially...
- 120
- 418
- 0
Báo cáo sinh học: "Beyond toxicity: aryl hydrocarbon receptor-mediated functions in the immune system" docx
Ngày tải lên :
06/08/2014, 19:21
... absent from TH1 and TH2 cells and only marginally present in TREG cells AhR expression also varies in other immune cells, including antigen-presenting cells such as dendritic cells and macrophages, ... multiple physiological ligands for AhR and it is likely that these bind with varying affinities and are rapidly degraded by induced metabolizing enzymes, thus providing transient and tightly regulated ... Amongst these ligands are dietary components - flavones and indoles, tryptophan metabolites as well as intrinsic factors such as prostaglandin subtypes, lipoxin A4, bilirubin and others [11] It...
- 3
- 335
- 0
Báo cáo khoa học: "The use of crossreactive monoclonal antibodies to characterize the immune system of the water buffalo (Bubalus bubalis)" pdf
Ngày tải lên :
07/08/2014, 15:20
... intestine and mammary gland [23] (and unpublished) ACT3 (WC10) [27] is differentially expressed on ab and gd T cells It is predominantly expressed on CD4+ cells following stimulation with Con A Expression ... differ phenotypically, in usage of Vγ, Jγ, Cγ, and Vδ gene segments, and in tissue distribution [1,14] The first subpopulation is CD2+, CD3+, CD5+, and CD6+ A subset of this subpopulation is CD8+ ... Clevers, H C and Davis, W C Clustering of monoclonal antibodies recognizing different members of the WC1 gene family Vet Immunol Immunopathol 1993, 39, 155-160 20 Morrison, W I and Davis, W C...
- 7
- 388
- 0
Báo cáo y học: "A crucial role for tumor necrosis factor receptor 1 in synovial lining cells and the reticuloendothelial system in mediating experimental arthritis" pps
Ngày tải lên :
12/08/2014, 12:20
... seconds at 95°C and minute at 60°C Gene expression (cycle threshold, Ct) values were normalized using glyceraldehyde-3-phosphate dehydrogenase (Gapdh) as a reference gene (ΔCt = Ctgene - CtGapdh) ... RNAi-mediated downregulation of gene expression involves both translational repression and accelerated mRNA turnover [19] To investigate the efficiency of TNFR1 gene silencing by shRNA expression, we transduced ... fore and hind paws was monitored at indicated time points and scored for severity (c) Histological analysis of inflammation ('infiltrate' and 'exudate') and proteoglycan depletion in patellar and...
- 11
- 319
- 0
Báo cáo y học: "Science Review: Vasopressin and the cardiovascular system part 1 – receptor physiology" doc
Ngày tải lên :
12/08/2014, 19:22
... balland-stick representation and the receptor is shown in ribbons The intracellular loops of the receptor are labeled il1, il2, and il3, and the extracellular loops are labeled el1, el2, and ... which binds to all types of V1R and V2R, certain OTRs, and neurophysins When selective V1R and V2R radioligands with in vitro autoradiography were used to study V1R and V2R binding sites, no binding ... involved in osmotic and cardiovascular homeostasis, whereas oxytocin is important in parturition, lactation, and sexual behavior Oxytocin and vasopressin are encoded by separate genes but they lie...
- 8
- 378
- 0
Báo cáo y học: "Science Review: Vasopressin and the cardiovascular system part 2 – clinical physiology" pps
Ngày tải lên :
12/08/2014, 19:22
... 50 and 100 pg/ml vasopressin but fell at 400 and 500 pg/ml The maximal peak ventricular pressure and dP/dtmax responses were at 50 pg/ml, whereas at 500 pg/ml both peak ventricular pressure and ... septic shock and vasodilatory states associated with cardiac anesthesia and surgery We stress that the clinical studies to date have been small and have focused on physiologic outcomes, and data ... such as hypoxia, acidosis, and increased nitric oxide [13] KATP channels can be depolarized (closed) by vasoconstrictors such as vasopressin and angiotensin II [16] GRP, gene- related protein example,...
- 9
- 333
- 0