nonviral approaches for cancer gene therapy

... them to serve as "Trojan horses" for the delivery of genes for cancer therapy Indeed, clostridial spores that were genetically manipulated to harbour genes for cancerstatic factors, prodrug converting ... commonly studied and most widely used system in cancer gene therapy They are of particular utility for cancer gene therapy applications, where temporary gene expression is acceptable or even beneficial ... vectors for cancer gene therapy At present, there are various gene therapy vector systems under development against cancer However, due to the complexity of the solid tumours involving angiogenesis,...

Ngày tải lên: 14/08/2014, 19:22

... Abbreviations XIV Chapter Introduction 1.1 Gene therapy in cancer treatment 1.1.1 Gene therapy strategies for cancer treatment 1.1.1.1 Suicide gene therapy 2 1.1.1.1.1 Herpes Simplex Virus Thymidine ... Gene therapy to inhibit pro-angiogenic pathways 12 1.1.1.6.2 Gene therapy to stimulate anti-angiogenic pathways 12 1.1.2 Gene carriers for cancer therapy 13 1.1.2.1 Viral vectors 13 1.1.2.2 Nonviral ... for cancer gene therapy 24 1.2.3 New reliable sources for therapeutic stem cells 26 1.3 Current mouse models in cancer research 28 1.3.1 Syngeneic models 28 1.3.2 Xenogeneic models 29 1.3.3 Genetically...

Ngày tải lên: 09/09/2015, 10:08

... viral gene therapy approaches Adenoviruses and Adeno-Associated viruses are used to deliver genes to tumor cells with the goal of modifying the phenotype, as for example, by introducing suicide genes ... important for the resistance to possible introduction of genes using viral vectors or for the resistance to oncolytic gene therapy We believe that this finding can be of crucial interest for the ... P, Kreike B, et al: An interferon-related gene signature for DNA damage resistance is a predictive marker for chemotherapy and radiation for breast cancer Proc Natl Acad Sci USA 2008, 105:18490-18495...

Ngày tải lên: 18/06/2014, 16:20

... vectors in gene therapy for glioma Finally, this study demonstrated, for the first time, that human embryonic stem cells can provide a potentially unlimited source for glioma gene therapy Using ... vectors for glioma gene therapy 4.4 Discussion 141 Chapter Conclusions 146 Reference 151 VII SUMMARY Malignant glioma remains one of the most lethal forms of cancer in humans However, current therapy ... cell vectors in glioma gene 20 therapy 1.3.6 Stem cell based glioma gene therapy 1.3.6.1 Therapeutic genes 22 22 1.3.6.1.1 Prodrug-converting enzymes 22 1.3.6.1.2 Other gene payloads 25 1.3.6.2...

Ngày tải lên: 11/09/2015, 09:00

... target for selective gene therapy As gene therapy for GBM is localised there is minimum risk of systemic toxicity (Pulkkanen and Yla-Herttuala, 2005) Gene therapy is the transfer of exogenous genes, ... of survival Generally there is no curative therapy for GBM and long-term control is rarely achieved with current therapies (Pulkkanen and Yla-Herttuala, 2005) 3    1.2 Gene Therapy for GBM Although ... for Glioblastoma .3 1.2 Gene therapy for GBM 1.3 Transcriptional Targeting 1.3.1 Tissue-specific promoters .7 1.3.2 Tumour-specific promoters 1.4 Vectors for Gene...

Ngày tải lên: 22/10/2015, 21:20

... vectors for gene therapy formed by self-assembly of DNA with synthetic block co-polymers Hum Gene Ther 7, 2123–2133 11 Kabanov, A V and Kabanov, V A (1995) DNA complexes with polycations for the ... Molecular Medicine, vol 65: Nonviral Vectors for Gene Therapy Edited by: M A Findeis © Humana Press Inc., Totowa, NJ Maruyama and Takei lized by SECs The authors are therefore interested in HA as ... and 17 amino acid residues were sufficient to form aggregates with the DNA, From: Methods in Molecular Medicine, vol 65: Nonviral Vectors for Gene Therapy Edited by: M A Findeis © Humana Press...

Ngày tải lên: 11/04/2014, 09:59

... outperform other methods in classification performance for small gene subsets while selecting genes that have plausible relevance to cancer diagnosis After formally stating the problem and reviewing ... performance for various classifiers all trained with genes selected by the method of (Golub, 1999) In contrast, the SVM selected genes yield consistently better performance than the baseline genes ... or ALL There is therefore a lot of redundancy in this gene set In essence, all the genes carry the same information Conversely, the SVM selected genes carry complementary information This is reflected...

Ngày tải lên: 06/03/2014, 00:22

... horse for vaccine development and cancer gene therapy Curr Gene Ther 2010, 10:56-76 Kim-Schulze S, Kaufman HL: Gene therapy for anti-tumor vaccination In Methods in Molecular Biology, Gene Therapy ... Dendrimer-based nanoparticles for cancer therapy Nanotechnology for Hematology 2009, 708-719 64 Chatterjee DK, Zhang Y: Multi-functional nanoparticles for cancer therapy Science and Technology ... nanoparticles for the detection, analysis and treatment of cancer have been mentioned Many applications in vaccine therapy or gene therapy are listed as following: Bolhassani et al Molecular Cancer...

Ngày tải lên: 06/03/2014, 02:21

... a cancer gene therapy strategy Hum Gene Ther 9, 185–193 Fang J, Sawa T, Akaike T & Maeda H (2002) Tumortargeted delivery of polyethylene glycol-conjugated d-amino acid oxidase for antitumor therapy ... d-amino acid oxidase (DAAO) from Rhodotorula gracilis (EC 1.4.3.3) for cancer treatment [11] Subsequently, the strategy for cancer therapy based on oxystress and DAAO was implemented using, in addition, ... of oxystress for cancer therapy J Drug Target 15, 475–486 Niculescu-Duvaz I & Springer CJ (2005) Introduction to the background, principles, and state of the art in suicide gene therapy Mol Biotechnol...

Ngày tải lên: 16/03/2014, 02:20

... suicide gene therapy Antiangiogenic gene therapy Virotherapy Virotherapy Measles virus Virotherapy Measles and mumps viruses Virotherapy Adenovirus AAV Delivery of p73 HSV-TK suicide gene therapy ... recurrent ovarian cancer Cancer Gene Ther 9: 553 – 566 Hasenburg, A., et al (2000) Thymidine kinase gene therapy with concomitant topotecan chemotherapy for recurrent ovarian cancer Cancer Gene Ther ... study of adenovirus–p53 gene therapy for recurrent ovarian cancer Cancer Gene Ther 10: 224 – 238 Zeimet, A G., and Marth, C (2003) Why did p53 gene therapy fail in ovarian cancer? Lancet Oncol 4:...

Ngày tải lên: 22/03/2014, 11:20

... Therefore, an effective non-pharmacological therapy for cancer with less cost and no side effects could have a major impact on cancer treatment Qigong therapy from TCM shows promise in treating cancer ... rates were 83% and 17% for lung cancer patients (the control was 7% in years); 83% and 23% for stomach cancer (the control was 12% in years) The median survival period for liver cancer patients was ... anti-tumor mechanism of Qigong therapy in a study of 104 different cancer patients (mainly comprised of esophagus cancer, stomach cancer, rectum cancer and lung cancer) These patients were taught...

Ngày tải lên: 22/03/2014, 17:20

... of target gene expression or gene silencing can also be performed using miRNA, siRNA or shRNA expression vectors [1] Gene therapy is useful for both genetic and acquired diseases For genetic diseases, ... Introduction Gene therapy is a promising approach to treat intractable and refractory diseases at the genetic level Basically, in gene therapy, target gene expression is induced by delivering foreign genes ... fibrosis [3] Gene therapy clinical trials were also performed for acquired diseases such as cancers, cardiovascular diseases and infectious diseases [3] There are two strategies to perform gene therapy, ...

Ngày tải lên: 23/03/2014, 14:20

... highlight the relevance of CD44v isoforms in cancer which seem to be suitable targets for anti -cancer therapy In several primary and cancer cells, CD44v6 forms a ternary complex with HGF and ... repair and gene therapy [106], when they are appropriately engineered for therapy We established that tissue-specific floxed plasmid ⁄ nanoparticle delivery is efficient for the activation of a gene of ... growth factor, transforming growth factorb), to recruit MSCs to form the supporting stroma of the tumor, and also pericytes for angiogenesis MSCs transfected with the interferon-b gene can increase...

Ngày tải lên: 28/03/2014, 23:20

... PROGENITOR CELLS AS TARGETS FOR GENE THERAPY 143 144 GENE THERAPY FOR HEMATOLOGICAL DISORDERS restoration of cellular and humoral immunity were documented after gene therapy Data showed a surprisingly ... otherwise standard MuLV vectors for gene therapy has not been successful Beyond these HEMATOPOIETIC STEM AND PROGENITOR CELLS AS TARGETS FOR GENE THERAPY 137 efforts, there are obviously major ... approaches In vivo gene transfer is most appropriate for target cells that cannot REQUIREMENTS FOR GENE TRANSFER INTO HEMATOPOIETIC CELLS 135 TABLE 6.1 Relevant Targets and Applications for Gene...

Ngày tải lên: 10/04/2014, 22:10

... hand, it is necessary for any vehicle used for in 156 GENE THERAPY FOR LIVER DISEASE vivo hepatic gene therapy to reach the liver efficiently For systemic application, the gene vectors are ideally ... attempted so far GENERAL PRINCIPLES FOR HEPATIC GENE THERAPY There are two basic approaches for gene transfer into hepatocytes: ex vivo and in vivo strategies (Fig 7.1) Ex vivo therapy requires ... Chiang YL Adenovirus-mediated gene therapy of hepatocellular carcinoma using cancerspecific gene expression Cancer Res 55:5283–5287, 1995 Schuster MJ, Wu GY Gene therapy for hepatocellular carcinoma:...

Ngày tải lên: 10/04/2014, 22:11

... competes for an essential substrate or co-factor that is available in limiting amounts, or, for proteins that form multimeric complexes, the 270 GENE THERAPY FOR HIV INFECTION TABLE 11.1 Gene Therapy ... gene expression Thus, the antisense gene expression must be quantatively higher than the levels of HIV-1 gene expression for an antisense gene therapy strategy to be effective Standard gene therapy ... levels in HSCs Thus, retroviral-mediated gene transfer is currently the optimal gene transfer system available for use in HIV-1 gene therapy The 282 GENE THERAPY FOR HIV INFECTION limitations of retroviral...

Ngày tải lên: 10/04/2014, 22:11

... be a mechanism for carcinogenesis in inherited neoplasms or cancers appearing in ontogeny GENE THERAPY APPROACHES TO THE TREATMENT OF CANCER One strategy in the gene therapy of cancer is the compensation ... that play a role in pathogenesis Numerous oncogenes have been targeted for antisense gene therapy They include c-fos for brain cancer, c-src for colon cancer, c-myb for leukemia and tumors of ... Vile RG Different approaches in the gene therapy of cancer Forum (Geneva) 9:225–236, 1999 Hall, SJ, Chen S-H, Woo SLC The promise and reality of cancer gene therapy Am J Hum Genet 61:785–789,...

Ngày tải lên: 10/04/2014, 22:11

... Adenovirus-Mediated Targeted Gene Therapy for Breast Cancer and for Purging Hematopoietic Stem-Cell Sources Ling Chen 361 23 Chemotherapy-Inducible Vector for Gene Therapy of Cancer Wolfgang Walther, ... Ribozyme for Gene Therapy of Breast Cancer Toshiya Suzuki, Masami Bessho, and Kevin J Scanlon 247 15 Anti-K-ras Ribozyme Adenoviral Vector for Gene Therapy of Non-Small Cell Lung Cancer Yu-An ... Therefore, since it is a disease characterized by aberrant gene expression, cancer has been a target of gene therapy research since the inception of this treatment modality Numerous cancer gene therapy...

Ngày tải lên: 11/04/2014, 09:42

... loci (for review, see refs 5,8) Recombinant DNA technology can also be employed for generating human MAbs from human lymphocyte mRNA The genetic information for antibody variable regions is generally ... retained is generally unique for each reshaped antibody and can therefore be difficult to foresee Such approach was successfully used for humanizing a MAb 4D5 against the product of protooncogene HER2 ... antibodies as therapeutic agents in cancer therapy This review provides a brief historical sketch of the development of recombinant antibodies for immunotherapy of cancer, which is followed by the...

Ngày tải lên: 11/04/2014, 10:25

... paper) Gene therapy Laboratory manuals Genetic vectors–Laboratory manuals Transfection–Laboratory manuals Viral genetics–Laboratory manuals I Machida, Curtis A II Series [DNLM: Genetic Vectors Gene ... vectors for gene transfer and gene therapy I would like to thank the many chapter authors for their contributions They are all experts in various aspects of viral vectors, and I appreciate their efforts ... Type 1-Based Vectors for Gene Delivery to Human Hematopoietic Stem Cells Ali Ramezani and Robert G Hawley 467 Semliki Forest Viral Vectors for Gene Transfer Jarmo Wahlfors and Richard A Morgan...

Ngày tải lên: 11/04/2014, 10:39