nmr studies of proteins at material interfaces

... the synthesis of proteins in the presence of other proteins provided in excess at the start of or during the reaction, e.g., for the purpose of rescuing nascently produced insoluble proteins into ... promise for accelerated studies of proteins by NMR spectroscopy by assigning residue type information to every amide cross-peak observed in [15N]-HSQC spectra We anticipate that high yield cell-free ... selective isotope labelling of less efficiently produced proteins, a combinatorial labelling strategy can be adopted Combinatorial labelling minimizes the number of samples that need to be prepared...

Ngày tải lên: 07/03/2014, 12:20

... reduced state (C) of the N(10) atom of TrxR-bound FAD appears at 161.5 p.p.m., 2.0 p.p.m upeld from that of free FMN (Table 3, Fig 6A) implicating an increase of p-electron density at the N(10) atom ... comparison to those of TARF The 13 C chemical shift of C(4a) of wild-type protein resonates at the same eld as that of FMN In the mutant proteins the chemical shift of the C(4a) atom appears at a higher ... are still at lower eld than that of FMN but at slightly higher eld than that of protein-bound FAD in wild-type enzyme As shown by model studies, polarization of the isoalloxazine ring of avin through...

Ngày tải lên: 16/03/2014, 16:20

... the pantoate-bound form at full occupancy indicates that the backbone Ca atoms show ˚ an overall deviation of 0.51 A Pantoate bound at site I The physicochemical nature of binding of pantoate to ... O1 atom of pantoate in site I The O4 atom of pantoate in site II and the O1 atom of pantoate in site I are hydrogen-bonded via a water molecule Structural stabilization of ligands by networks of ... chains of the residues that line the active site pantoate The Nd atom of Asp152 is hydrogen-bonded via one water molecule to the O3 atom of pantoate, and via two water molecules to the O4 atom of...

Ngày tải lên: 16/02/2014, 09:20

... removal of unsaturated chain termini from tetrasaccharides For removal of the nonreducing terminal unsaturated uronic acids [26], an aliquot of reduced tetrasaccharide mixture was incubated at room ... MHz NMR studies of human articular cartilage keratan sulfates Eur J Biochem 266, 1174– 1183 Huckerby TN & Lauder RM (2000) Keratan sulfates from bovine tracheal cartilage structural studies of ... the analysis of CS ⁄ DS composition These studies have yielded crucial data allowing an understanding of species, tissue, age and pathology related differences and the estimation of changes in...

Ngày tải lên: 16/03/2014, 14:20

... studies on SAM-coated electrodes have demonstrated that very high electric fields induce a conformational transition from the native form (denoted as state B1) to the conformational state (state ... excess of negative or positive charges can be created by using protonable tail groups, and are particularly suited for electrostatic immobilization of soluble proteins In the case of the highly cationic ... different kinds of information about the interfacial processes of cytochrome c First, the unique vibrational signatures of reduced and oxidized haems allows the two oxidation states of the immobilized...

Ngày tải lên: 22/03/2014, 16:20

... of these studies are discussed within the context of the general mechanism of the onset of pathologic states upon Ab aggregation Results NMR spectroscopy Interaction with zinc ) heterocorrelation ... dissociation constant of copper ions, and indirectly to estimate the dissociation constant of zinc 52 Table Dissociation constants of copper and zinc for Ab The dissociation constants were calculated ... aromatic region of the 1H–13C HSQC spectrum is particularly informative about the effects of Zn2+ (Fig 2) The Tyr10 aromatic resonances are unchanged upon zinc addition, indicating that the aromatic...

Ngày tải lên: 23/03/2014, 10:20

... a circulating water bath, and acidification was corrected by automated additions of 10% NH4OH NMR operation Using cells at high density (> g of DCWÆL)1), 31P NMR signals were recorded at 161 MHz ... I were estimated based on 31P NMR data The data shown are the mean ± SD Fig Changes in cellular ATP, ADP and ATP/ADP of the XMP overproducer in the production phase (A) Cellular ATP (s) and ADP ... the average ATP plus ADP concentration raised 58% of that in the wild-type strain, but ATP concentration further decreased to an undetectable level (Table 2) Thus, low ATP levels or ATP plus ADP...

Ngày tải lên: 23/03/2014, 17:22

... many of them either often played truant or did their private things during listening lessons That state resulted in their very low marks or even failure in the examination of English That was ... number of 59.9% and it is followed by the skill of awareness of word stress which is illustrated by 56.6% Surprisingly, sound discrimination skill is put at the bottom of the list with only 23.3% of ... information about the author of the song, the root of it and even the words of the song So there are no reasons for not making advantage of such a useful and potential source of teaching material...

Ngày tải lên: 29/01/2014, 10:33

... possible that it follows an alternative mechanism, i.e the deprotonation and reprotonation of C2¢ The alternative reaction can be initiated by deprotonation of C1¢ and protonation of N5 to form ... the association rate constant of HPO (0.55 lm)1Æs)1) was very similar to that of HP (0.65 lm)1Æs)1), but the dissociation rate constant of HPO (0.062 s)1) was about a quarter of that of HP (0.26 ... compilation ª 2007 FEBS 2249 Mechanism and kinetics of dihydroneopterin aldolase Y Wang et al 5¢-GGAATTCCATATGGATATTGTATTTATAGAGCA AC-3¢ (forward primer with a Nde I site) and 5¢-CGGGA TCCTTAATTATTTTCTTTCAGATTATTGCC-3¢...

Ngày tải lên: 19/02/2014, 00:20

... our studies of the epitope of MBP therefore we may conclude that the conformation of epitopes of the integral protein must be affected by posttranslational modifications Docking calculations of ... Comparison of the conformation of active and nonactive backbone cyclic analogs of substance P as a tool to elucidate features of the bioactive conformation: NMR and molecular dynamics in DMSO and water ... case of the antagonist simulations) were coupled separately to a temperature bath with a time constant of 0.1 ps The pressure was coupled to an external bath at 100 kPa with a time constant of...

Ngày tải lên: 07/03/2014, 16:20

... opinion be something that could run on a computer using the technology of today or at least tomorrow m to ensure that the influence of the situation does not invalidate the use of data and results when ... large part of a communicative event breaks the rules of communication should lead to a questioning of the validity of the formulated rules Perhaps present day analysis of the structure of language ... of our results 58% of the Initiatives are Context Independent, i.e utterances that can be interpreted in isolation However, of these about 10% are dialogue openings This means that only 48% of...

Ngày tải lên: 18/03/2014, 02:20

... saturation with ms intervals and a total saturation time of  s On-resonance irradiation of the protein was conducted at a chemical shift of –0.4 p.p.m and off-resonance at a chemical shift of ... Structure of b-Me-Gal and the relative degree of saturation of individual protons normalized to that of the H4 proton as determined from the 1D STD NMR spectrum (B) decay values with on- and off-resonance ... using NMR titration experiments From the theoretical calculations of Kd for the ratios of sugar-free to sugarbound peak intensities as a function of sugar concentration, the protein concentration...

Ngày tải lên: 23/03/2014, 04:21

... indicated that VP51 and VP76 were located on the viral capsid, suggesting that they are structural components of the viral capsid Localization of VP51 and VP76 by FCM It is well known that FCM ... anti-VP664 serum) by immunofluorescence flow cytometry Data are expressed as the means ± standard deviation of three independent experiments Materials and methods Preparation of intact WSSV virions ... remaining bound proteins The low-pH treated sample was suspended in 0.2 ml of TM buffer Expression and purification of proteins VP51 and VP76 are the products of ORF wsv308 and wsv220 of WSSV (GeneBank...

Ngày tải lên: 20/06/2014, 01:20

... matches the experimental ratio Rexp is the one that a sublayer is made of The experimental ratios Rexp of the HAADF intensity of sublayer of the nominal GaAs layer to those of the GaAs substrate, ... region of the investigated sample compared with that (B) of a sample exhibiting the expected GaAs QW emission CL at 77 K with keV beam energy Page of the peak B of the GaAs QW (1.56 eV at 77 K) ... information on the nature and composition of sublayers and Figure is the plot of the calculated intensity ratio between In 0.15 Ga 0.85 As 1-y P y and GaAs It shows that the experimental value of...

Ngày tải lên: 21/06/2014, 05:20

... superposition of elastic behavior of ssDNA and dsDNA at a percentage of replication of p = 0.7 All figures reprinted from Proc Natl Acad Sci USA 97, 12002 (2000) Copyright 2006 by National Academy of Sciences, ... of the motion of the stained proteins when they encountered the DNA molecules One possible future application of stretching DNA molecules across electrode gaps is a fabrication of a network of ... stretching starts at the tip of pointed electrode at V (B) Greater numbers of stretched DNA molecules covering a greater area are observed near the tip of the pointed electrode at 12 V (C) DNA...

Ngày tải lên: 22/06/2014, 18:20

... measurement of pure liquids and liquid mixtures The viscometer was calibrated with fresh conductivity water immersed in a water bath that was maintained at the experimental temperature The flow time of ... self-associated liquids with a three-dimensional network of hydrogen bonds9 and can be associated with any other group having some degree of polar attraction.10 A survey of the literature has shown that ... strongly associated with hydrogen bonding, with an extent of polymerisation that may differ depending on temperature, chain length and position of the OH group Due to the polar natures of DMF, cyclohexanone...

Ngày tải lên: 07/08/2014, 14:20

... reduced at room temperature demonstrating that incubation at a higher temperature reduced unspecific attachment of this virus to CHO cells, while attachment to CHO-CAR, CHO-DAF and HeLa remained at ... Similar observations were reported by Pash et al [31], where two strains of Figure Attachment studies using a clinical isolate of CVB5 Attachment studies using a clinical isolate of CVB5 A) × ... TCID50/cell) of CVB2O and attachment carried out at 4°C or at room temperature (Figure 2B) showed that increasing the temperature is an important parameter using our experimental setup Binding of CVB2O...

Ngày tải lên: 12/08/2014, 04:21

... on a native polyacrylamide gel resulted in the separation of nine proteins When each of these proteins, designated as native proteins (NPs) through 9, was isolated from the native gel, denatured, ... regulated defense gene expression These results, taken together, demonstrate the degree of complexity in the regulation of defense signaling pathways 1.5 Study of Strawberry’s Pathogenesis-Related ... stage of development Osmotin-like protein was also identified as one of the PR proteins related to the somatic embryogenesis of Cichorium (Helleboid et al., 2000) These studies demonstrate that...

Ngày tải lên: 13/11/2014, 09:25

... hybridization of a carbon atom (b) carbon atoms making a giant macromolecular array (lattice) in diamond 24 Figure 3-2 (a) sp2 hybridization of a carbon atom (b) configuration of carbon atoms ... Comparison of the friction coefficient of (a) ta-C coated AlTiC substrate without pre-treatment, (b) ta-C coated AlTiC substrate with pre-treatment, and (c) bare AlTiC substrate in ball on flat rotary ... profile of the ta-C coating of the Head-3 (with Al-Si-C interlayer) on (a) unworn area, and (b) AlTiC substrate after 340 km wear test 126 Figure 8-10 Depth profile of the ta-C coating of...

Ngày tải lên: 09/09/2015, 10:17