nitric oxide synthase in retinal vascular diseases
Báo cáo khoa học: "Enhanced expression of constitutive and inducible forms of nitric oxide synthase in autoimmune encephalomyelitis" pdf
Ngày tải lên :
07/08/2014, 14:22
... of nitric oxide synthase in autoimmune encephalomelitis 27 28 29 30 31 32 33 34 35 36 37 38 Induction of nitric oxide synthase activity in human astrocytes by interleukin-1beta and interferon-g ... NOS including nNOS, eNOS, and iNOS were increased in the initiation of EAE and suggested that the brain cells including neurons and astrocytes are possible sources for either nNOS or eNOS in the ... the inflammatory cells in the pathogenesis of EAE, as does iNOS in EAE [45] However, our study did not support the finding of EAE in iNOS knockout mice in which both nNOS and eNOS were not increased...
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Báo cáo khoa học: "Immunohistochemical study of constitutive neuronal and inducible nitric oxide synthase in the central nervous system of goat with natural listeriosis" potx
Ngày tải lên :
07/08/2014, 14:23
... observed in inflammatory microabscesses Counterstained with hematoxylin Magnification: ×132 C: Immunohistochemical staining of inducible NOS in the caprine brain with natural listeriosis shows that iNOS ... immunoreactivity was recognized in neurons, some inflammatory cells in perivascular cuffings Counterstained with hematoxylin, Magnification: ×132 D: Immunostaining of neuronal NOS in brain sections of goats ... reacted for 60 min, followed by biotinylated antisera (Zymed) for 15 minutes and [strept]avidin- biotin peroxidase (Zymed) for 15 minutes Staining was done using a LAB-SA Kit (Zymed) according to the...
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Báo cáo y học: "Gender-based reciprocal expression of transforming growth factor-β1 and the inducible nitric oxide synthase in a rat model of cyclophosphamide-induced cystitis" ppsx
Ngày tải lên :
11/08/2014, 08:22
... urine at multiple time points in studies assessing inflammatory analytes in urine In contrast to earlier studies, we now demonstrate interrelated NO2-/NO3- and TGF-β1 levels in individual urine ... acrolein-injured bladder Given the interplay of regulatory influences operating in the production of NO, TGF-β1 and other pro-inflammatory cytokines in bladder inflammation, we sought to define ... 10-fold higher than levels in female urine Panel B Urine levels of TGF-β-1 after CYP Injection of CYP induced time dependent 100-fold increase in urine levels of TGF-β1 in rats of both sexes relative...
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Báo cáo y học: " Proinflammatory role of inducible nitric oxide synthase in acute hyperoxic lung injury" pot
Ngày tải lên :
12/08/2014, 18:21
... homodimeric proteins consisting of a N-terminal oxygenase domain, a central calmodulin binding sequence, and a C-terminal reductase domain [21,22] Inducible NOS is expressed following induction by ... nitration of tyrosine in proteins, for example the reaction of myeloperoxidase with hydrogen peroxide Therefore, increased nitrotyrosine staining is considered as an indicator of "increased nitrative ... decreased total protein count, LDH activity, and nitrotyrosine staining in iNOS-deficient mice [27] In contrast, Cytokines may also play a role in oxygen toxicity Several studies point out that TNF-α...
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Báo cáo y học: "Involvement of Akt and endothelial nitric oxide synthase in ventilation-induced neutrophil infiltration: a prospective, controlled animal experiment" docx
Ngày tải lên :
13/08/2014, 08:20
... [14,15] Nitric oxide has been shown to be produced from Larginine by a family of nitric oxide synthase isoforms, including inducible nitric oxide synthase and eNOS, which are expressed in a wide ... without hyperoxia are complex, including activation of mitogen-activated protein kinases [12], serine/threonine kinase/protein kinase B (Akt), and endothelial nitric oxide synthase (eNOS) [14,15] High ... effects on stretch-induced Akt and endothelial nitric oxide synthase activation Phosphorylated serine/threoactivation nine kinase/protein kinase B (Akt) or endothelial nitric oxide synthase (eNOS)...
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Tài liệu Báo cáo khoa học: Polarized distribution of inducible nitric oxide synthase regulates activity in intestinal epithelial cells pdf
Ngày tải lên :
19/02/2014, 16:20
... enrichment in iNOS activity within the particulate fraction of epithelial cells Apical distribution of iNOS in intestinal epithelial cells To get more insight into the localization of iNOS in intestinal ... localization of iNOS to apical domains of intestinal epithelial cells Particulate fraction association of iNOS in vivo In order to determine the distribution of iNOS in intestinal epithelial cells in vivo, ... which, in intestinal cells, could be bacteria present in the intestinal lumen This hypothesis is supported by positioning of iNOS on the apical side of intestinal crypts Recently PDZ-binding a2...
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Báo cáo khoa học: Interference with the citrulline-based nitric oxide synthase assay by argininosuccinate lyase activity in Arabidopsis extracts docx
Ngày tải lên :
07/03/2014, 05:20
... lines on the autoradiogram The origin was marked with an ink spot Argininosuccinate is the immediate precursor to arginine in the urea cycle, and is converted to arginine and fumarate by argininosuccinate ... NOS assay Citrulline Hydroxyarginine Argininosuccinate Ornithine Origin 1-D 2-D Fig Two-dimensional TLC analysis of reaction product A reaction with [14C]arginine, desalted protein extract and ... arginine that mimics an NOS reaction This reaction, however, produces argininosuccinate, not citrulline, and requires fumarate, indicating that ASL is catalyzing the reaction Because argininosuccinate...
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Báo cáo khoa học: Rapamycin inhibits lipopolysaccharide induction of granulocyte-colony stimulating factor and inducible nitric oxide synthase expression in macrophages by reducing the levels of octamer-binding factor-2 doc
Ngày tải lên :
22/03/2014, 17:20
... and by inhibiting eukaryotic translation initiation factor 4E-binding protein (eIF4E-BP1) [19] Rapamycin, a potent immunosuppressor, exerts its function by binding to the FK506-binding protein (FKBP12) ... macrophages, and the involvement of Oct-2 in LPS ⁄ mTOR-induced iNOS and G-CSF expression was investigated further Results Rapamycin inhibits the LPS-induced increase in Oct-2 protein levels in RAW264.7 ... mTOR inhibitor, rapamycin In addition, Oct-2 is involved in the expression of LPS-inducible genes that contain octamer in their promoters [24,25] Thus, rapamycin may inhibit the expressions of iNOS...
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Báo cáo khoa học: In vivo degradation of nitric oxide synthase (NOS) and heat shock protein 90 (HSP90) by calpain is modulated by the formation of a NOS–HSP90 heterocomplex pot
Ngày tải lên :
30/03/2014, 04:20
... of inducible nitric oxide synthase (iNOS) by calpain I Biochim Biophys Acta 1568, 216– 224 23 Su Y & Block ER (2000) Role of calpain in hypoxic inhibition of nitric oxide synthase activity in ... previously to induce an increase in the level of [Ca2+]i and a correlated calpain activation [28] This increase in [Ca2+]i is more intense in aorta than in brain Under these conditions, in brain, no ... amount of nitric oxide Am J Physiol Renal Physiol 285, 178–190 In vivo degradation of NOS and HSP90 by calpain Kone BC (2000) Protein–protein interactions controlling nitric oxide synthases Acta...
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Báo cáo khoa học: Thermodynamic and kinetic analysis of the isolated FAD domain of rat neuronal nitric oxide synthase altered in the region of the FAD shielding residue Phe1395 docx
Ngày tải lên :
30/03/2014, 14:20
... F1395S NOS, suggesting interplay between CaM binding, nicotinamide binding and F1395 in coenzyme binding/FAD reduction [29] The switch in coenzyme specificity towards NADH in CPR is consistent ... the FAD isoalloxazine ring nNOS, rat neuronal nitric oxide synthase; eNOS, rat endothelial nitric oxide synthase; iNOS, rat inducible nitric oxide synthase; CPR, human cytochrome P450 reductase; ... indicating that Trp676 is actively involved in displacing NADP+ from reduced FAD domain [27] Mutagenesis of the aromatic shielding tyrosine residue in pea ferredoxinNADP+ reductase to serine stabilizes...
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báo cáo hóa học: " Signaling pathways mediating a selective induction of nitric oxide synthase II by tumor necrosis factor alpha in nerve growth factor-responsive cells" pptx
Ngày tải lên :
19/06/2014, 22:20
... cytokines of the inducible nitric oxide synthase promoter in insulin-producing cells Diabetologia 1998, 41:1101-8 Kong GY, Peng ZC, Costanzo C, Kristensson K, Bentivoglio M: Inducible nitric oxide ... derived neurotrophic factor; IGF, insulin-like growth factor; IL-1β, interleukin-1beta; iNOS, inducible nitric oxide synthase; MAPK, mitogen activated protein kinase; NF-κB, nuclear factor kappa ... absence blots detecting iNOS in total NGF in A: Western blots detecting iNOS in total protein extracts from PC12 cells treated for 24 hr with increasing concentrations of NGF in the presence or...
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Báo cáo khoa học: "Expression of nitric oxide synthase isoforms in the porcine ovary during follicular development" ppsx
Ngày tải lên :
07/08/2014, 18:21
... and inducible (iNOS) isoforms of nitric oxide synthase (NOS) in the ovaries of pigs The intensity of staining is indicated by (-), where staining was absent, up to (+++), for maximal staining ... The involvement of nitric oxide in the ovulatory process in the rat Endocrinology 1994, 135, 2287-2290 11 Takesue K, Tabata S, Sato F, Hattori MA Expression of nitric oxide synthase- 3 in porcine ... of inducible and endothelial nitric oxide synthase in the rat ovary Effects of gonadotropin stimulation in vivo J Clin Invest 1995, 96, 2719-2726 15 Xie QW, Nathan C The high-output nitric oxide...
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Báo cáo khoa học: " Effects of L-NAME, a non-specific nitric oxide synthase inhibitor, on AlCl3-induced toxicity in the rat forebrain cortex" doc
Ngày tải lên :
07/08/2014, 23:22
... epinephrine autoxidation Anal Biochem 1978, 90, 81-89 35 Tanino H, Shimohama S, Sasaki Y, Sumida Y, Fujimoto S Increase in phospholipase C-delta1 protein levels in aluminum-treated rat brains ... AlCl3 administration; (4) L-NAME group (n = 10) - animals were -4 treated with L-NAME with single dose (1 × 10 g dissolved in saline) before saline administration Using a stereotaxic instrument ... group (Fig 1) Superoxide anion radical production in the rat forebrain cortex The results in Fig show the bilateral ∙O2 levels (μM red NBT/min/mg proteins) in the rat forebrain cortex homogenates...
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Báo cáo y học: "Signalling pathway involved in nitric oxide synthase type II activation in chondrocytes: synergistic effect of leptin with interleukin-1" pdf
Ngày tải lên :
09/08/2014, 06:22
... were incubated for 48 hours with both cytokines in the presence of the NOS type II inhibitor aminoguanidine (1 mmol/l), added hour before cytokine administration Aminoguanidine completely inhibited ... Figure Janus kinase (JAK)2 inhibition blocks leptin/IL-1-induced nitric oxide (NO) production and nitric oxide synthase (NOS) type II protein expression expression Effect of tyrphostin AG490 and ... Nelson AH, Barone FC, Kumar S: SB 203580 inhibits p38 mitogenactivated protein kinase, nitric oxide production, and inducible nitric oxide synthase in bovine cartilage-derived chondrocytes J Immunol...
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Báo cáo y học: "Octacalcium phosphate crystals directly stimulate expression of inducible nitric oxide synthase through p38 and JNK mitogen-activated protein kinases in articular chondrocytes" pps
Ngày tải lên :
09/08/2014, 06:23
... protein kinases and tyrosine kinases on IL1-induced NF-κB activation and iNOS expression in bovine articular chondrocytes Nitric Oxide 2002, 6:35-44 66 LaPointe MC, Isenovic E: Interleukin-1β ... dehydrogenase; iNOS, inducible nitric oxide synthase; JNK, c-Jun amino-terminal kinase; NO, nitric oxide; OCP, octacalcium phosphate Figure OCP crystal induced p38 and JNK MAPK activation Nonadherent bovine ... ankyrin repeat containing a serine/threonine protein kinase that interacted with the cytoplasmic domain of β1 integrin [51] Furthermore, Liu and Lioté [37] found that, within a few minutes, MSU crystals...
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Báo cáo y học: "Dynamic compression counteracts IL-1β induced inducible nitric oxide synthase and cyclo-oxygenase-2 expression in chondrocyte/agarose constructs" pdf
Ngày tải lên :
09/08/2014, 10:23
... Nelson AH, Barone FC, Kumar S: SB203580 inhibits p38 mitogenactivated protein kinase, nitric oxide production, and inducible nitric oxide synthase in bovine cartilage-derived chondrocytes J Immunol ... noted for iNOS induction in response to IL-1β, with a sevenfold increase in free-swelling conditions (Figure 2a), as compared with a 145-fold increase in unstrained constructs cultured within the ... marked differences in the diffusional constraints in unstrained constructs in the bioreactor, involving contact of the upper surface with a fluid impermeable loading pin Accordingly, the mass transport...
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Báo cáo y học: "Protective effects of total fraction of avocado/soybean unsaponifiables on the structural changes in experimental dog osteoarthritis: inhibition of nitric oxide synthase and matrix metalloproteinase-13" docx
Ngày tải lên :
09/08/2014, 14:20
... Kanno K, Saito I, Miyasaka N: Nitric oxide production and inducible nitric oxide synthase expression in inflammatory arthritides J Clin Invest 1995, 96:2357-2363 41 McInnes IB, Leung BP, Field M, ... in an ovine OA model induced by meniscectomy [9] In addition to the above findings, and most interesting are the results from clinical trials that have shown a beneficial effect of ASU on clinical ... Nitric oxide mediates interleukin-1 induced inhibition of glycosaminoglycan synthesis in rat articular cartilage Mediators Inflamm 1995, 4:107-111 48 Murrell GAC, Jang D, Williams RJ: Nitric oxide...
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Heme oxygenase-1 plays a pro-life role in experimental brain stem death via nitric oxide synthase I/protein kinase G signaling at rostral ventrolateral medulla pps
Ngày tải lên :
10/08/2014, 05:21
... I/PKG signaling in RVLM Illustrative gels or summary of fold changes against aCSF controls in ratio of nitric oxide synthase I (NOS I), protein kinase G (PKG), NOS II or nitrotyrosine (NT) relative ... [13] identified in RVLM during brain stem death is heat shock protein 70 (HSP70), which ameliorates cardiovascular regulatory dysfunction via enhancing NOS I/PKG signaling and inhibiting NOS II/peroxynitrite ... selective activation of HO1 in RVLM is causally involved in central cardiovascular regulation during brain stem death Pretreatment with microinjection bilaterally into RVLM of an anti-HO-1 antiserum...
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Báo cáo y học: " Protein kinase A-dependent Neuronal Nitric Oxide Synthase Activation Mediates the Enhancement of Baroreflex Response by Adrenomedullin in the Nucleus Tractus Solitarii of Rats" pps
Ngày tải lên :
10/08/2014, 05:21
... Neuronal nitric oxide synthase activation is involved in insulin-mediated cardiovascular effects in the nucleus tractus solitarii of rats Neuroscience 2009, 159:727-734 Prast H, Philippu A: Nitric oxide ... in NTS AC: adenylate cyclase; ADM: adrenomedullin; ADMR: adrenomedullin receptor; GC: guanylate cyclase; Gs: stimulatory GTP-binding protein; nNOS: neuronal nitric oxide synthase; PKA: protein ... increase in protein levels of phospho-nNOS (Ser1416) in NTS The ADM-induced increase in phosphorylation of nNOS at Ser1416 maximized at 30 and gradually declined within 60 after ADM administration...
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