new methods and approaches

Ngày tải lên: 16/03/2014, 17:20

... standardized, and should include a minimum wall filter setting of 50 Hz and a Doppler sample volume between and mm (Szatmari et al., 2001) 4 Hemodynamics – New Diagnostic and Therapeutic Approaches ... difference caused by them can be 12 Hemodynamics – New Diagnostic and Therapeutic Approaches observed In benign neoplasia, on the other hand, no specific and measurable Doppler flow spectrum has been ... complex 16 Hemodynamics – New Diagnostic and Therapeutic Approaches interactions between several regulatory pathways (renin–angiotensin, kallikrein–kinin, and prostaglandin–thromboxane) are in...

Ngày tải lên: 19/02/2014, 23:20

... secreted and bound to the hepatocyte Gene Polymorphism and Coronary Heart Disease surface and readily released by heparin It is homologous to LPL and pancreatic lipase and hydrolyzes TGs and phospholipids ... Disease – New Insights and Novel Approaches either induce a prothrombotic/anti-fibrinolytic effect as Apo A resembles both plasminogen and plasmin but has no fibrinolytic activity, and/ or may ... heart 4 Coronary Artery Disease – New Insights and Novel Approaches The coronary artery system consists of two main arteries, the left and right arteries, and their marginal arterial network...

Ngày tải lên: 08/03/2014, 00:20

... standardized, and should include a minimum wall filter setting of 50 Hz and a Doppler sample volume between and mm (Szatmari et al., 2001) 4 Hemodynamics – New Diagnostic and Therapeutic Approaches ... HEMODYNAMICS – NEW DIAGNOSTIC AND THERAPEUTIC APPROACHES Edited by A Seda Artis Hemodynamics – New Diagnostic and Therapeutic Approaches Edited by A Seda Artis Published ... difference caused by them can be 12 Hemodynamics – New Diagnostic and Therapeutic Approaches observed In benign neoplasia, on the other hand, no specific and measurable Doppler flow spectrum has been...

Ngày tải lên: 14/03/2014, 10:20

... + μb , (1) σPAN where μPAN and μb are the mean of the PAN and MS image band b, respectively, and s PAN and s b are the standard deviation of the PAN and MS image band b, respectively This technique ... Price and spatially adaptive methods, Bayesian-based and super-resolution methods (5) Multiresolution family, which includes generalized Laplacian pyramid, wavelet and contourlet methods and any ... finding a new component Cl and adding the difference between the PAN and this new component to each upsampled MS image band This framework has been further extended by Wang et al.[14] and Aiazzi...

Ngày tải lên: 20/06/2014, 22:20

... Nondestructive Testing Methods and New Applications, Edited by Mohammed Omar p cm ISBN 978-953-51-0108-6 Contents Preface IX Part Chapter Part General Nondestructive Testing Methods and Considerations ... areas such as physics, mathematics and biology MMC has been used a 10 Nondestructive Testing Methods and New Applications long time in order to obtain numeric approaches of complex functions This ... used for training and certification of NDT personnel 12 a Nondestructive Testing Methods and New Applications Fatigue cracks For metal alloys, fatigue cracks are initiated and grown under controlled...

Ngày tải lên: 28/06/2014, 17:20

Ngày tải lên: 18/07/2014, 22:48

... twenty standard amino acids can be classified into several groups: non12 polar aliphatic (G, A, V, L, M and I), non-polar aromatic (F, Y and W), polar acidic (L, R and H), polar basic (D and E) and ... presents a couple of new methods using molecular simulation, bioinformatics and statistical analysis to study the structural ensemble and sequences of proline-rich disordered regions A new approach, ... specified in the resonance spectrum The deviation from random coil to helix and beta strand conformations can be determined by tables of chemical shift, and these inform us of evidence of local secondary...

Ngày tải lên: 09/09/2015, 10:14

... Nodes Q, I, and E) and nodes in I1 ∪ I2 [1, 0, 0, 0] and [0, 0, 0, 1] (i.e., (i.e., Nodes R, T, U, and W) Among the nodes, only cases (a), (b), (d), and (f ) pass the feasibility and optimality ... properties of the problems at hand, the exact methods like Combinatorial Benders' Cuts algorithm and Local Branching method have been developed and tested to be promising methods for the complex problems ... randomly generated and solved by CPLEX and proposed GRASPs, respectively Both GRASP_1 and GRASP_2 are coded in Matlab and executed in a Pentium (R) IV PC with 3.00GHz CPU and 2.00 GB of RAM Due...

Ngày tải lên: 10/09/2015, 15:48

... a UV monitor and collect fractions using a fraction collector and subject to the appropriate carbohydrate and antibody analyses 3.6 Analysis of Mucins Methods for the detection and analysis of ... antibodies and lectins or coating methods such as the glycan detection method and enzyme-linked immunosorbent assays (ELISA) All these techniques have advantages and disadvantages Solution methods ... three principally different methods are available: solution assays such as colorimetric assays for hexose and sialic acid; membrane-based methods such as slot-blotting and staining with periodic...

Ngày tải lên: 08/10/2012, 10:25

... mammary gland, defined by antibodies to cytokeratins J Cell Sci 75, 17–33 Kiernan, J A (1990) Histological and Histochemical Methods: Theory and Practice, Pergamon, Oxford, UK Andrew, S M and Jasani, ... M and Milton, J D (1986) Lectin Methods and Protocols, Humana, Totowa, NJ Liener, I E., Sharon, N., and Goldstein, I J (1998) The Lectins: Properties, Functions, and Applications in Biology and ... such as Culling’s Handbook of Histopathological and Histochemical Techniques (including museum techniques) (36) or Kiernan’s Histological and Histochemical Methods: Theory and Practice (45) provide...

Ngày tải lên: 08/10/2012, 10:25

... core protein specificity), and lower sensitivity than slot-blot and immunoassay methods Chap- Detection and Quantitation of Mucins 47 ter (2) discusses these assays and they are mentioned here ... greater sample volume, and superior washing) For quantitation and comparison across blots, a standard in the same buffer as samples should be titrated for use as a standard curve, and samples should ... NJ Nagma, K., Thornton, D J., Khan, N., and Sheehan, J K (2000) Separation and identification of mucins and their glycoforms, in Glycoprotein Methods and Protocols: The Mucins (Corfield, T.,...

Ngày tải lên: 08/10/2012, 10:25

... and held together by bolts tightened by hand Millipore filter paper to act as a backing for the mucosa during sectioning and subsequent manipulation of the section 2.2 Histological Staining and ... (w/v) poly-L-lysine for and dried at room temperature Methods 3.1 Observation and Measurement of Mucous Thickness on Unfixed Mucosal Sections (see Notes 1–4) This is a rapid and simple method for ... layer will be greatly reduced and discontinuous Careful handling of the cryostat sections on the slide during fixing and staining is important because excessive washing and so on can remove mucus...

Ngày tải lên: 08/10/2012, 10:25

... PhosphorImager The identification of mucin precursors and mature mucins using polypeptide-specific antisera and SDS-PAGE is elaborated in Chapters 20 and 21, and in several references (1,4–11) The precursor ... Strous, G J and Dekker, J (1992) Mucin-type glycoproteins Crit Rev Biochem Mol Biol 27, 57–92 Van Klinken, B J W., Dekker, J., Büller, H A., and Einerhand, A W C (1995) Mucin gene structure and expression ... Einerhand, A W C., and Dekker, J (1995) Biosynthesis of intestinal mucins: MUC1, MUC2, MUC3, and more Biochem Soc Trans 23, 814–818 11 Van Klinken, B J W., Einerhand, A W C., Büller, H A., and Dekker,...

Ngày tải lên: 08/10/2012, 10:25

... a small gel, use 1.6 g of agarose and 160 mL of buffer, and for a large gel, use 2.8 g of agarose and 280 mL of buffer Leave to cool before pouring (hand hot) and insert well-forming comb (see ... are 1.5 mm thick and cm wide There is a compromise between band broadness and amount of sample to be loaded Larger amounts of sample tend to yield poorer quality data; i.e., the bands become more ... I., and Sheehan, J K (1996) Identification of glycoproteins on nitrocellulose membranes and gels Mol Biotechnol 5, 171–176 10 Thornton, D J., Howard, M., Devine, P L and Sheehan, J K (1995) Methods...

Ngày tải lên: 08/10/2012, 10:25

... microscopy of biopolymers Biopolymers 9, 373–379 96 Sheehan and Thornton 12 Koller, T., Harford, A G., Lee, Y K., and Beer, M (1969) New methods for the preparation of nucleic acid molecules for ... Kadler, K E., and Chapman J A (1985) Mica sandwhich technique for preparing macromolecules for rotary shadowing J Ultrastruct Res 91, 66–76 14 Kawahara K and Tanford C (1966) Viscosity and density ... into 0.5-mL fractions, and these were analyzed for reactivity with antisera for the MUC5AC (᭹) and MUC5B (᭺) mucins The MUC5B mucin is more polydisperse than the MUC5AC mucin and has molecules of...

Ngày tải lên: 08/10/2012, 10:25

... 114 Yan and Packer chromatography, and leads to a significantly lower recovery of all the amino acids, especially serine and threonine Strong acid, high temperature, and short time acid ... at least for pump and column equilibration with the initial composition of the mobile phase Figures and show typical chromatograms of the separation of amino acid standards and hydrolyzed bovine ... samples and Dr Andrew Gooley and Prof Keith Williams for their support References Yan, J X., Wilkins, M R., Ou, K., Gooley, A A., Williams, K L., Sanchez, J.-C., Golaz, O., Pasquali, C., and Hochstrasser,...

Ngày tải lên: 08/10/2012, 10:25

... Notes and 8) and the length of sequence obtained is dependent on the number and distribution of proline residues Proline cleaves with reduced efficiency in Edman sequencing (Fig 1, vessel 1), and ... I., Kirk, M., Barnes, S., Lowary, T L., and Hart, G W (1996) Selective detection and site-analysis of O-GlcNAc-modified glycopeptides by β-elimination and tandem electrospray mass spectrometry Anal ... PerSeptive Biosystems and Beckman supply this product Quantitation of glycopeptides is quite difficult, and we recommend that 10% of the sample be first sequenced using the standard program prior...

Ngày tải lên: 08/10/2012, 10:25

... tandem repeat domain and overlapping each other by six amino acids (16), and the other used octamers overlapping each other by seven amino acids (17) Twelve antibodies were analyzed in total and ... information on antibody specificity and permits identification of significant features of an epitope that may contribute to antibody recognition and binding (see Notes 10 and 11) 1.3 Purification of ... must be pure and free from amines Several methods may be used to purify DMF (see Note 16) Piperidine 20% v/v: used for Fmoc deprotection Piperidine should be redistilled before use and made up...

Ngày tải lên: 08/10/2012, 10:25