... ê 2009 The Authors Journal compilation ê 2009 FEBS Solution structure of hirsutellin A – newinsightsinto theactive site and interacting interfaces of ribotoxinsAldino Viegas1, Elias Herrero-Gala´n2, ... recognition andribonucleolytic activity. J Mol Biol 299, 1061–1073.14 Yang X & Moffat K (1996) Insightsinto specificity ofcleavage and mechanism of cell entry from the crystalstructure of the highly ... the assignment process. The unambiguous NOEsassigned to a given pair of protons were converted into upper limits by cyana. Additionally, standard upper andlower limits for each of the two disulfide...
... using refmac5 [32] andCoot [35] until the free R factor and the crystallographicR factor could not be improved further. For calculation ofthe R free values, 1% (ThDP–ADP–EcODC and ThDP–ace-tyl ... Richards NG & Lindqvist Y (2007) Crystal-lographic snapshots of oxalyl-CoA decarboxylase give insights into catalysis by nonoxidative ThDP-dependentdecarboxylases. Structure 15, 853–861.5 Muller ... atoms 8798 9344 9037Number of atoms (protein) 8153 8280 8191Number of atoms (water) 515 907 707R free (%) 23.7 19.6 19.4Rwork(%) 19.3 17.7 17.5Average B factors (A˚2)Protein 36.55 19.17...
... activatorFEBS Journal 275 (2008) 39443958 ê 2008 The Authors Journal compilation ê 2008 FEBS 3947 Newinsightsinto the functions and N-glycan structures offactor X activator from Russell’s viper venomHong-Sen ... 5,397–409.43 Spina E, Sturiale L, Romeo D, Impallomeni G,Garozzo D, Waidelich D & Glueckmann M(2004) New fragmentation mechanisms inmatrix-assisted laser desorption/ionizationtime-of-flight/time-of-flighttandem ... 6) and advanced MS/MS(Fig. 7) analyses of the permethylated N-glycans, butwith a few important new findings.Overall, the salient structural characteristics of theN-glycans released from the...
... therefore:C ỵ Ciẳ Ci1 ỵ bị6ị478 C. Loetchutinat et al. (Eur. J. Biochem. 270) Ó FEBS 2003 Newinsightsinto the P-glycoprotein-mediated effluxesof rhodaminesChatchanok Loetchutinat, Chantarawan ... rhodamine bound to its receptors; Ciis theconcentration of free internal rhodamine; F: molar fluorescence(arbitrary units) of rhodamine free in the cytosol; q, fluorescencequantum yield of rhodamine ... poten-tial, is proportional to the amount of rhodamine free in thecytosol. This observation allows the further determinationof the concentration of drug free in the cytosol of resistantcells.The determination...
... Authors Journal compilation ê 2010 FEBS Cadmium – glutathione solution structures provide new insightsinto heavy metal detoxificationOlivier Delalande1,*, Herve´Desvaux2, Emmanuel Godat1,3, ... [29].Here we provide further insightsinto cadmium com-plexation by metabolites. We considered four glutathi-one-related peptides, GSH, c-Glu-Cys, a-Glu-Cys-Gly(a-GSH) and the free GSSG oxidized form. ... transport into the vacuole through Ycf1 or the export outsidethe cell through Yor1 or both. Using wild-type cellslabelled with35S-GSH, we observed that the totalexport of glutathione [including free...
... FEBS Journal 275 (2008) 1039–1052 ª 2008 FEBS. No claim to original French government works Newinsightsinto evolution of crustacean hyperglycaemichormone in decapods – first characterization in ... value is veryclose to the M + H+value of 8345.6 Da calculatedfrom the cDNA sequence and taking into account thepredicted post-translational maturation steps (forma-tion of three disulfide bridges, ... Seminar Series 65, pp. 33–52. Cambridge Univer-sity Press, Cambridge.15 Chang ES, Bruce MJ & Newcomb RW (1987) Purifica-tion and amino acid composition of a peptide withmolt-inhibiting activity...