neural differentiation of induced pluripotent stem cells
Expansion of Human Induced Pluripotent Stem Cells on Synthetic Substrate in Defined Medium
... TABLE OF CONTENTS CHAPTER INTRODUCTION .1 1.1 Stem cells 1.1.1 Human embryonic stem 1.1.2 Induced pluripotent stem cells 1.2 History of stem cell ... Medium Expansion of Human Induced Pluripotent Stem Cells on Synthetic Substrate in Defined Medium A thesis submitted in partial fulfillment of the requirements for the degree of Master of Science in ... Biology By Huantong Yao University of Nankai Bachelor of Science in Biotechnology, 2006 December 2012 University of Arkansas ABSTRACT Human induced pluripotent stem cells (hiPSCs) have the potential
Ngày tải lên: 04/11/2022, 07:33
Dissecting the retinoid‐induced differentiation of f9 embryonal stem cells by integrative genomics
... Macmillan Publishers Limited Dissecting RA -induced differentiation of F9 embryonal stem cells MA Mendoza-Parra et al 800 600 400 B Induced genes (no RXRα–RARγ) Induced genes; RXRα site ... impact of RAR and RXR signalling However, what has remained entirely enigmatic is how a single compound upon Molecular Systems Biology 2011 Dissecting RA -induced differentiation of F9 embryonal stem ... TF, transcription factor Molecular Systems Biology 2011 & 2011 EMBO and Macmillan Publishers Limited Dissecting RA -induced differentiation of F9 embryonal stem cells MA Mendoza-Parra et al and RXRa
Ngày tải lên: 24/11/2022, 17:40
probabilistic modeling of reprogramming to induced pluripotent stem cells
... systems utilizing different transcription factor combinations and cell fate conversion systems, such as the reprogramming of epiblast stem cells into embryonic stem cells or cellular transdifferentiation ... power of our methodology for investigating reprogramming and other cell fate change systems INTRODUCTION Somatic cells can be experimentally reprogrammed into induced pluripotent stem cells (iPSCs) ... 2Department SUMMARY Reprogramming of somatic cells to induced pluripotent stem cells (iPSCs) is typically an inefficient and asynchronous process A variety of technological efforts have been
Ngày tải lên: 04/12/2022, 16:03
Activation of wntβ catenin signalling via GSK3 inhibitors direct differentiation of human adipose stem cells into functional hepatocytes
... (hESCs) and human induced pluripotent stem cells (hiPSCs) Manipulations of Wnt signalling via glycogen synthase kinase (GSK3) inhibitors have been exploited to direct differentiation of definitive ... with activation of Wnt/β-catenin signalling Down regulation of the β-catenin expression attenuates the effect of GSK3 inhibitors on the induction of these specific genes The cells induced using ... December 2016 Published: 17 January 2017 Activation of Wnt/β-catenin signalling via GSK3 inhibitors direct differentiation of human adipose stem cells into functional hepatocytes Jieqiong Huang*,
Ngày tải lên: 19/11/2022, 11:45
hyaluronan induces odontoblastic differentiation of dental pulp stem cells via cd44
... mesenchymal cells, which retain the ability to differentiate into mature cells Induced pluripotent stem cells have been developed from various cell sources, including dental pulp-derived stem cells, ... pulp-derived induced pluripotent stem cells have been evaluated for use in clinical applications [32, 33], and HA stimulation of CD44 is important for differentiation leading to the production of odontoblasts, ... Wenceslau CV, Miglino MA, Muotri AR, Kerkis I Feeder-free derivation of induced pluripotent stem cells from human immature dental pulp stem cells Cell Transplant 2011;20(11-12):1707–19 24 25 26 27 Gronthos
Ngày tải lên: 04/12/2022, 10:38
Use of a Synthetic Xeno-Free Culture Substrate for Induced Pluripotent Stem Cell Induction and Retinal Differentiation
... derivation of retinal cells from normal mouse and human fibroblast-derived induced pluripotent stem cells (iPSCs) (A–F): Microscopic analysis of mouse (A–C) and human (D–F) iPSC-retinal precursor cells ... Figure Microscopic analysis of Synthemax -induced pluripotent stem cell (iPSC) potency (A–F): Histological analysis of Synthemax-iPSC generated teratomas for production of cells/ tissues specific to ... postdifferentiation photoreceptor and retinal ganglion precursor cells were present Figure Xeno-free derivation of retinal cells from a patient with retinitis pigmentosa (RP -induced pluripotent stem
Ngày tải lên: 18/10/2022, 01:27
derivation of neural progenitors and retinal pigment epithelium from common marmoset and human pluripotent stem cells
... obtaining donor neural retinal and RPE cells is required Previous studies have demonstrated the capacity of human embryonic stem cells (HESCs) and human -induced pluripotent stem cells (HIPSCs) ... embryonic stem cells, ” Stem Cells and Development, vol 20, no 9, pp 1587–1599, 2011 [10] P Chatterjee, Y Cheung, and C Liew, “Transfecting and nucleofecting human induced pluripotent stem cells, ” ... Yu et al., ? ?Neural differentiation of human induced pluripotent stem cells follows developmental principles but with variable potency,” Proceedings of the National Academy of Sciences of the United
Ngày tải lên: 01/11/2022, 09:48
identification of novel biomarkers for doxorubicin induced toxicity in human cardiomyocytes derived from pluripotent stem cells
... Human pluripotent stem cells Cardiomyocytes Doxorubicin Toxicity Biomarkers Introduction Abbreviations: cTnT, cardiac specific troponin T; hESC, human embryonic stem cells; hiPSC, human induced pluripotent ... induced pluripotent stem cells; hPSC, human pluripotent stem cells; LDH, lactate dehydrogenase; SAM, statistical analysis of microarray * Corresponding author at: University of Skövde, Box 408, ... www.elsevier.com/locate/toxicol Identification of novel biomarkers for doxorubicin -induced toxicity in human cardiomyocytes derived from pluripotent stem cells Gustav Holmgren a,b, * , Jane Synnergren
Ngày tải lên: 02/11/2022, 11:39
Differentiation of pluripotent stem cells into striatal projection neurons: a pure MSN fate may not be sufficient
... et al (2007a) A scaleable and defined system for generating neural stem cells from human embryonic stem cells Stem Cells 25, 731–737 doi: 10.1634/stemcells.2006-0562 Joannides, A J., Webber, ... potential for differentiation to essentially any mature fate; the principle sources being embryonic stem cells (ESCs) and/or induced pluripotent stem cells (iPSCs), followed by directed differentiation ... Embryonic stem cells neural differentiation qualifies the role of Wnt/betaCatenin signals in human telencephalic specification and regionalization Stem Cells 31, 1763–1774 doi: 10.1002 /stem. 1462
Ngày tải lên: 24/11/2022, 17:43
The march of pluripotent stem cells in cardiovascular regenerative medicine stem cell res ther 2018 9 1 p 201
... of stem cells, including embryonic stem cells (ESCs) and adult stem cells (ASCs), and focuses primarily on induced pluripotent stem cells (iPSCs) The key methods used for converting somatic cells ... potential of induced pluripotent stem cells J Cardiovasc Transl Res 2010;3(1):13–23 86 Blelloch R, Venere M, Yen J, Ramalho-Santos M Generation of induced pluripotent stem cells in the absence of drug ... potential of stem cells Differential potential Number of stem types Original stem cell Differentiated cells Totipotential All Fertilized egg (zygote) All cell types Pluripotential All except cells of
Ngày tải lên: 19/03/2021, 23:02
genotoxic effects of culture media on human pluripotent stem cells
... integrity of Human Pluripotent Stem Cells (HPSCs) We report that HPSCs cultured in Essential (E8) and mTeSR, two widely used media for feeder-free culturing of HPSCs, had many fold higher levels of ... pluripotency markers, differentiation into all three germ layers and normal karyotype through multiple passages, in designing culture media The capability of human pluripotent stem cells (HPSCs) to ... ‘quality’ of stem cells has been defined by robust expression of pluripotency markers, capability to differentiate into all the three germ layers, established by teratoma formation or in vitro differentiation,
Ngày tải lên: 04/12/2022, 10:32
generation of pdgfr cardioblasts from pluripotent stem cells
... percentage of cTnT+ cells; the optimal dose of each modulator was as follows: 3 μg/mL of CsA; 10 μM of Y27632; 400 μM of Trolox; and 1 μg/mL of EW7197 (Fig. S1B–E) The optimal dose of CsA, Y27632, ... identity of putative cardioblasts are still unclear Here, we uncover a novel class of cardiac lineage cells, PDGFRα+Flk1− cardioblasts (PCBs), from mouse and human pluripotent stem cells induced ... cause of mortality worldwide, with death arising from the inability of cardiomyocytes to regenerate after myocardial injury In this aspect, cardiac lineage cells (CLCs) from pluripotent stem cells
Ngày tải lên: 04/12/2022, 10:32
isolation and expansion of human pluripotent stem cell derived hepatic progenitor cells by growth factor defined serum free culture conditions
... stages of liver stem cells which have the capacity for unlimited proliferation and mutilineage differentiation, and such kind of liver stem cells change their expression markers in dependence of differentiation ... Hayakawa, M.K Furue, H Mizuguchi, Efficient generation of functional hepatocytes from human embryonic stem cells and induced pluripotent stem cells by HNF4alpha transduction, Mol Ther 20 (2012) ... regulates human pluripotent stem cell self-renewal, PLoS One (2013) e54122 [23] N.R Hannan, C.P Segeritz, T Touboul, L Vallier, Production of hepatocyte-like cells from human pluripotent stem cells, Nat
Ngày tải lên: 04/12/2022, 15:13
Screen of nuclear receptors for the enhanced and alternative generation of induced pluripotent stem cells
... restriction of differentiated cells is undone The resultant cells, known colloquially as induced pluripotent stem cells (iPSCs), become akin to pluripotent embryonic stem cells (ESCs) and pluripotent cells ... SCREEN OF NUCLEAR RECEPTORS FOR THE ENHANCED AND ALTERNATIVE GENERATION OF INDUCED PLURIPOTENT STEM CELLS DOMINIC HENG JIAN CHIEN (B.Sc (Hons), NTU) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF ... List of nuclear receptors screened for enhancers of reprogramming… … 62 Table Pluripotent assays of Nr5a2-reprogrammed cells …………………… …74 x List of Figures Figure The developmental potential of...
Ngày tải lên: 09/09/2015, 18:56
Generation of porcine induced pluripotent stem cells and their differentiation into cardiac lineages
... fibroblasts to Induced Pluripotent Stem Cells 45 iv 4.10 Culture of Porcine Induced Pluripotent Stem Cells 45 4.10 Characterization of Porcine Induced Pluripotent Stem Cells 46 4.10.1 Immunocytochemical ... Pluripotent Stem Cells ……………… 44 4.8 Infection of fibroblast cells to form Induced Pluripotent Stem Cells …………………………………………………………………… 45 4.9 Culture of cells transitioning from fibroblasts to Induced ... embryonic stem cells have been isolated, induced pluripotent stem cells for the pig must be used as a source of pluripotent cells Hence, fibroblasts from the pig is taken, and induced into pluripotent...
Ngày tải lên: 30/09/2015, 10:13
Báo cáo y học: "Hypertrophy is induced during the in vitro chondrogenic differentiation of human mesenchymal stem cells by bone morphogenetic protein-2 and bone morphogenetic protein-4 gene transfer" pps
... chondrogenic differentiation of mesenchymal progenitor cells and to up regulate the levels of type II collagen and aggrecan in chondrocytes and chondroprogenitor cells [1,7-11] During development of the ... 6-carboxyfluorescein by the esterases of living cells to label viable cells This combination allows the differentiation among early apoptotic cells (Ann5 positive, 6-CFDA positive), necrotic cells (Ann5 positive, ... arcades of Benninghoff [29] and by the appearance of hypertrophic cells Analyses of lacunae surface areas in the different growth plate zones revealed mean lacunae surface areas ± SD of 100.8...
Ngày tải lên: 09/08/2014, 14:22
báo cáo khoa học: "Epigenomics of human embryonic stem cells and induced pluripotent stem cells: insights into pluripotency and implications for disease" potx
... embryonic stem cell; H3K4me3, trimethylation of lysine of histone H3; H3K27ac, acetylation of lysine 27 of histone H3; H3K27me3, trimethylation of lysine 27 of histone H3; iPSC, induced pluripotent stem ... embryonic stem cell; H3K4me1, methylation of lysine of histone H3; H3K4me3, trimethylation of lysine of histone H3; H3K27ac, acetylation of lysine 27 of histone H3; H3K27me3, trimethylation of lysine ... Chin MH, Pellegrini M, Plath K, Lowry WE: Molecular analyses of human induced pluripotent stem cells and embryonic stem cells Cell Stem Cell 2010, 7:263-269 94 Deng J, Shoemaker R, Xie B, Gore...
Ngày tải lên: 11/08/2014, 12:21
Báo cáo khoa học: Recruitment of transcription complexes to the b-globin locus control region and transcription of hypersensitive site 3 prior to erythroid differentiation of murine embryonic stem cells docx
... erythroid differentiation of murine ES cells in vitro In these experiments we utilized the ES ⁄ OP9 cell in vitro differentiation system described by Kitayima et al [20] The ability of these cells ... locus during the differentiation of hematopoietic stem cells to erythroid cells Transcription of HS3 could be important in maintaining this accessible structure, particularly in light of the fact ... cases except that of the Rex-1 gene, primer sets span introns Day cells are composed of ES cells and MEF cells grown in ES media containing leukemia inhibitory factor (LIF) These cells express the...
Ngày tải lên: 07/03/2014, 12:20
Atlas of Human Pluripotent Stem Cells doc
... expansion of human embryonic stem cells, and more recently, of human induced pluripotent stem cells The exploration and realization of the incredible potential of these pluripotent stem cells for ... reprogramming of adult skin fibroblasts into induced pluripotent stem cells (Takahashi and Yamanaka, 2006) To realize the full potential of embryonic and induced pluripotent stem cells, technologies, ... culture, expansion and manipulation of human pluripotent stem cells The culmination of two events has enabled the production of human embryonic stem cells: the birth of the first IVF test-tube baby...
Ngày tải lên: 22/03/2014, 09:20
báo cáo hóa học:" MicroRNA and gene expression patterns in the differentiation of human embryonic stem cells" doc
... purposes) Journal of Translational Medicine 2009, 7:20 in the differentiation process of undifferentiated hES cells to neural progenitor cells and then declined upon further differentiation [25]; ... functions of the miR-17 through 92 family of miRNA clusters Cell 2008, 132(5):875-886 Kasashima K, Nakamura Y, Kozu T: Altered expression profiles of microRNAs during TPA -induced differentiation of ... Adult cells and EB shared genes (B) hES cells upregulated genes (C) EB upregulated genes (D) Adult cells upregulated genes hESC cells EB adult cells Figure supervised hierarchical clustering of...
Ngày tải lên: 18/06/2014, 15:20