neural differentiation of induced pluripotent stem cells

Screen of nuclear receptors for the enhanced and alternative generation of induced pluripotent stem cells

Ngày tải lên : 09/09/2015, 18:56

... restriction of differentiated cells is undone The resultant cells, known colloquially as induced pluripotent stem cells (iPSCs), become akin to pluripotent embryonic stem cells (ESCs) and pluripotent cells ... SCREEN OF NUCLEAR RECEPTORS FOR THE ENHANCED AND ALTERNATIVE GENERATION OF INDUCED PLURIPOTENT STEM CELLS DOMINIC HENG JIAN CHIEN (B.Sc (Hons), NTU) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF ... List of nuclear receptors screened for enhancers of reprogramming… … 62 Table Pluripotent assays of Nr5a2-reprogrammed cells …………………… …74 x List of Figures Figure The developmental potential of...

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Generation of porcine induced pluripotent stem cells and their differentiation into cardiac lineages

Ngày tải lên : 30/09/2015, 10:13

... fibroblasts to Induced Pluripotent Stem Cells 45 iv 4.10 Culture of Porcine Induced Pluripotent Stem Cells 45 4.10 Characterization of Porcine Induced Pluripotent Stem Cells 46 4.10.1 Immunocytochemical ... Pluripotent Stem Cells ……………… 44 4.8 Infection of fibroblast cells to form Induced Pluripotent Stem Cells …………………………………………………………………… 45 4.9 Culture of cells transitioning from fibroblasts to Induced ... embryonic stem cells have been isolated, induced pluripotent stem cells for the pig must be used as a source of pluripotent cells Hence, fibroblasts from the pig is taken, and induced into pluripotent...

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Báo cáo y học: "Hypertrophy is induced during the in vitro chondrogenic differentiation of human mesenchymal stem cells by bone morphogenetic protein-2 and bone morphogenetic protein-4 gene transfer" pps

Ngày tải lên : 09/08/2014, 14:22

... chondrogenic differentiation of mesenchymal progenitor cells and to up regulate the levels of type II collagen and aggrecan in chondrocytes and chondroprogenitor cells [1,7-11] During development of the ... 6-carboxyfluorescein by the esterases of living cells to label viable cells This combination allows the differentiation among early apoptotic cells (Ann5 positive, 6-CFDA positive), necrotic cells (Ann5 positive, ... arcades of Benninghoff [29] and by the appearance of hypertrophic cells Analyses of lacunae surface areas in the different growth plate zones revealed mean lacunae surface areas ± SD of 100.8...

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báo cáo khoa học: "Epigenomics of human embryonic stem cells and induced pluripotent stem cells: insights into pluripotency and implications for disease" potx

Ngày tải lên : 11/08/2014, 12:21

... embryonic stem cell; H3K4me3, trimethylation of lysine of histone H3; H3K27ac, acetylation of lysine 27 of histone H3; H3K27me3, trimethylation of lysine 27 of histone H3; iPSC, induced pluripotent stem ... embryonic stem cell; H3K4me1, methylation of lysine of histone H3; H3K4me3, trimethylation of lysine of histone H3; H3K27ac, acetylation of lysine 27 of histone H3; H3K27me3, trimethylation of lysine ... Chin MH, Pellegrini M, Plath K, Lowry WE: Molecular analyses of human induced pluripotent stem cells and embryonic stem cells Cell Stem Cell 2010, 7:263-269 94 Deng J, Shoemaker R, Xie B, Gore...

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Báo cáo y học: " Induced pluripotent stem cells free of exogenous reprogramming factors" pot

Ngày tải lên : 14/08/2014, 21:20

... approach for the generation of induced pluripotent stem cells Cell Stem Cell 2008, 2:525-528 Shi Y, Desponts C, Do JT, Hahm HS, Schöler HR, Ding S: Induction of pluripotent stem cells from mouse embryonic ... JA: Induced pluripotent stem cell lines derived from human somatic cells Science 2007, 318:1917-1920 Okita K, Nakagawa M, Hyenjong H, Ichisaka T, Yamanaka S: Generation of mouse induced pluripotent ... fibroblasts to induced pluripotent stem cells Nature 2009, 458:766-770 Kaji K, Norrby K, Paca A, Mileikovsky M, Mohseni P, Woltjen K: Virus-free induction of pluripotency and subsequent excision of reprogramming...

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Báo cáo khoa học: Recruitment of transcription complexes to the b-globin locus control region and transcription of hypersensitive site 3 prior to erythroid differentiation of murine embryonic stem cells docx

Ngày tải lên : 07/03/2014, 12:20

... erythroid differentiation of murine ES cells in vitro In these experiments we utilized the ES ⁄ OP9 cell in vitro differentiation system described by Kitayima et al [20] The ability of these cells ... locus during the differentiation of hematopoietic stem cells to erythroid cells Transcription of HS3 could be important in maintaining this accessible structure, particularly in light of the fact ... cases except that of the Rex-1 gene, primer sets span introns Day cells are composed of ES cells and MEF cells grown in ES media containing leukemia inhibitory factor (LIF) These cells express the...

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Atlas of Human Pluripotent Stem Cells doc

Ngày tải lên : 22/03/2014, 09:20

... expansion of human embryonic stem cells, and more recently, of human induced pluripotent stem cells The exploration and realization of the incredible potential of these pluripotent stem cells for ... reprogramming of adult skin ﬁbroblasts into induced pluripotent stem cells (Takahashi and Yamanaka, 2006) To realize the full potential of embryonic and induced pluripotent stem cells, technologies, ... culture, expansion and manipulation of human pluripotent stem cells The culmination of two events has enabled the production of human embryonic stem cells: the birth of the ﬁrst IVF test-tube baby...

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báo cáo hóa học:" MicroRNA and gene expression patterns in the differentiation of human embryonic stem cells" doc

Ngày tải lên : 18/06/2014, 15:20

... purposes) Journal of Translational Medicine 2009, 7:20 in the differentiation process of undifferentiated hES cells to neural progenitor cells and then declined upon further differentiation [25]; ... functions of the miR-17 through 92 family of miRNA clusters Cell 2008, 132(5):875-886 Kasashima K, Nakamura Y, Kozu T: Altered expression profiles of microRNAs during TPA -induced differentiation of ... Adult cells and EB shared genes (B) hES cells upregulated genes (C) EB upregulated genes (D) Adult cells upregulated genes hESC cells EB adult cells Figure supervised hierarchical clustering of...

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Báo cáo y học: "Chordin knockdown enhances the osteogenic differentiation of human mesenchymal stem cells" pdf

Ngày tải lên : 09/08/2014, 10:23

... number of cells was determined spectrophotometrically entiation of human mesenchymal transcripts Expression of BMP-2 and chordinstem cells during osteogenic differentiation of human mesenchymal stem ... Kassem M: Inhibition of osteoblast differentiation but not adipocyte differentiation of mesenchymal stem cells by sera obtained from aged females Bone 2006, 39:181-188 Page of (page number not ... suppression of chordin led to an increase in the bioavailability of endogenously produced BMP-2 to drive the differentiation of Page of (page number not for citation purposes) Effect of chordin...

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Fibronectin and laminin promote differentiation of human mesenchymal stem cells into insulin producing cells through activating Akt and ERK pptx

Ngày tải lên : 10/08/2014, 05:21

... vitro differentiation of size-sieved stem cells into electrically active neural cells Stem Cells 2002, 20(6):522-529 Tzeng SF, Tsai MJ, Hung SC, Cheng H: Neuronal morphological change of size-sieved ... and survival of adherent cells [17] FN and LAM constitute the major ECM of pancreas islet cells Laminin-1 has been reported to promote the differentiation of fetal mouse pancreatic b -cells [18] ... reporting the involvement of ERK or Akt in ECM or biomaterialsinduced enhancement of IPC differentiation Although, when bone marrow-derived endothelial cells [25] or stem cells [26] were transplanted,...

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báo cáo khoa học: " Capturing Alzheimer’s disease genomes with induced pluripotent stem cells: prospects and challenges" pps

Ngày tải lên : 11/08/2014, 12:21

... the isolation of neural stem cells, glia and neurons derived from human pluripotent stem cells PLoS One 2011, 6:e17540 48 Funato H, Yoshimura M, Yamazaki T, Saido TC, Ito Y, Yokofujita J, Okeda ... monogenic and complex diseases with pluripotent stem cells Nat Rev Genet 2011, 12:266-275 33 Gonzalez F, Boue S, Belmonte JCI: Methods for making induced pluripotent stem cells: reprogramming a la carte ... GQ, Porteus MH, Joung JK, Cheng L: Gene targeting of a disease-related gene in human induced pluripotent stem and embryonic stem cells Cell Stem Cell 2009, 5:97-110 41 Hockemeyer D, Soldner F,...

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Engineered poly(l lactic acid) based nanofibers for osteogenic differentiation of human mesenchymal stem cells

Ngày tải lên : 09/09/2015, 10:07

... the osteogenic differentiation of MSCs investigate the influences of prolonged culture as well as the nanofibrous structure on osteogenic differentiation function of the stem cells To produce ... 43 2.3 Stem cells 51 2.3.1 Definition and classification 51 2.3.2 Stem cells in tissue engineering 53 2.3.3 Mesenchymal stem cells 54 v 2.4 Stem cell ... Table 2.9 Classification of stem cells [23-30] 51 Table 2.10 Advantages and disadvantages of fabrication methods of nanofibers 79 Table 2.11 Clinical trials using nanofibers 88 Table...

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Applying macromolecular crowding to promote the expansion and adipogenic differentiation of human mesenchymal stem cells in vitro; an effect of matrix reciprocity

Ngày tải lên : 11/09/2015, 09:16

... osteogenic differentiation of human mesenchymal stem cells Exp Cell Res (1):22-37 101 Salasznyk RM, Klees RF, Boskey A, et al 2007;Activation of FAK is necessary for the osteogenic differentiation of ... 2009;Mesenchymal stem cell modification of endothelial matrix regulates their vascular differentiation J Cell Biochem (4):706-713 104 Kuhn NZ, Tuan RS 2010;Regulation of stemness and stem cell niche of mesenchymal ... 1991;Expression of modified bFGF cDNAs in mammalian cells Ann N Y Acad Sci :149-160 120 Kolf CM, Cho E, Tuan RS 2007;Mesenchymal stromal cells Biology of adult mesenchymal stem cells: regulation of niche,...

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Directed differentiation of human embryonic stem cells into haematopoietic and definitive endodermal lineages

Ngày tải lên : 04/10/2015, 16:03

... analysis of developmental events was made possible with the isolation of embryonic stem cells Extensive investigation of the properties and capabilities of these cells has led to the development of ... stimulation of regulatory T (Treg) cells in the host This mechanism in part can be attributed to the differentiation of the mixed pool of donor haematopoietic cells into allogeneic antigen-presenting cells ... CD83 that enhance the activation of naive T cells 14 Figure 1.5 Haematopoietic development Pluripotent stem cells give rise to multipotent haematopoietic stem cells that differentiate into lymphoid...

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Báo cáo y học: "Biology of adult mesenchymal stem cells: regulation of niche, self-renewal and differentiation" potx

Ngày tải lên : 09/08/2014, 10:20

... the induction of MSC adipogenesis Myogenesis Most investigations of myogenesis in adult stem cells are based on a small population of skeletal muscle-derived stem cells, or satellite cells A recent ... involvement of Wnts is complicated by their pleiotropic effects Examples of canonical Wnt functions include the promotion of long-term culture expansion of stem cells, increased in vivo reconstitution of ... complexes specific for tenogenic differentiation MSC niche In analyzing the differentiation of stem cells, it is critical to consider the influence of their tissue of origin MSCs are now routinely...

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báo cáo khoa học: " Transcriptomic analysis of pluripotent stem cells: insights into health and disease" potx

Ngày tải lên : 11/08/2014, 12:21

... programs of human embryonic and induced pluripotent stem cells Cell Stem Cell 2010, 7:249-257 Wilson KD, Venkatasubrahmanyam S, Jia F, Sun N, Butte AJ, Wu JC: MicroRNA profiling of human -induced pluripotent ... application of induced pluripotent stem cell technology for therapeutic purposes Patient-derived somatic cells can be isolated through tissue biopsies and converted into induced pluripotent stem cells ... dysregulation of gene expression [29] ESC, embryonic stem cell; hiPSC, human induced pluripotent stem cell; iPSC, induced pluripotent stem cell; miRNA, microRNA complex to control neural crest...

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Báo cáo y học: "Discrepancy between the in vitro and in vivo effects of murine mesenchymal stem cells on T-cell proliferation and collagen-induced arthritis" doc

Ngày tải lên : 12/08/2014, 11:23

... of 11 Mesenchymal stem cell treatment has no effect on the development of collagen -induced arthritis Figure Mesenchymal stem cells (MSCs) inhibit the anti-CD 3induced proliferation of CD4+ T cells ... Page of 11 Figure Mesenchymal stem cells (MSCs) inhibit the proliferation of CD4 + T cells in vitro by induction of nitric oxide and prostaglandin E2 (PGE2) (a) CD4+ T cells, in the presence of ... proliferation of CD4+ T cells was analyzed by detection of CFSE dilution by flow cytometry The percentage inhibition (100 × [(percentage of proliferating CD4+ cells not treated with MSCs – percentage of...

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Báo cáo y học: "Low temperature tolerance of human embryonic stem cells"

Ngày tải lên : 31/10/2012, 16:57

... bioreactors Conflicts of interest The authors have declared that no conflict of interest exists References Becker EW The roles of ATP in the dynamics of the actin filaments of the cytoskeleton ... Bongso A An efficient and safe xeno-free cryopreservation method for the storage of human embryonic stem cells Stem Cells 2004;22(5):779-89 10 Speicher MR, Ballard SG, Ward DC Karyotyping human ... expression of SSEA-3 was indicated by green fluorescence under a wavelength of 490 nm (FITC), while positive expression of TRA-1-81 was indicated by red fluorescence under a wavelength of 570 nm...

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Báo cáo sinh học: " Phosphoproteomic analysis of apoptotic hematopoietic stem cells from hemoglobin E/b-thalassemia" pot

Ngày tải lên : 18/06/2014, 19:20

... isolation of relatively pure populations of these cells for in vitro study of the cellular processes in thalassemic stem cells While previous attempts have focused on the cell culture system, in ... The isolated HSCs/CD34+ cells were harvested for phosphoproteomic analysis and cultivation of erythroid cells The cultivation of erythroid cells derived from HSCs/CD34+ cells was performed according ... the elucidation of signal transduction pathways, the understanding of the mechanism of disease progression, and the development of therapeutic applications [12] The phosphoproteome of HSCs in HbE/b-thalassemic...

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báo cáo hóa học:" Elevated adipogenesis of marrow mesenchymal stem cells during early steroid-associated osteonecrosis development" pptx

Ngày tải lên : 20/06/2014, 00:20

... trabecular bone area); fat cells diameter = the total diameter of fat cells in selected field/the number of fat cells in selected field; fat cells area = the area of all fat cells in selected field/(the ... marrow lipid was a consequence of the adipogenesis of marrow mesenchymal stem cells (MSCs) [7] Results of a previous study showed increased number of small size fat cells in the early steroid-associated ... temporal change of bone marrow fat cells in the process of steroid-associated-osteonecrosis development Chinese Journal of Osteoporosis 2007 Cui Q, Wang GJ, Balian G: Pluripotential marrow cells produce...

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