... containing 1% SDS, 10 mm EDTA, 50 mm Tris ⁄ HCl, pH Lysates were centrifuged at 14 000 g for 10 at °C, and the supernatant obtained was diluted 10 -fold using IP buffer [0. 01% SDS, 1. 1% Triton X -10 0, 1. 2 ... (2009) 7386–7399 ª 2009 The Authors Journal compilation ª 2009 FEBS D Mizgalska et al 10 11 12 13 14 15 16 17 18 disorder in proteins from the three kingdoms of life J Mol Biol 337, 635–645 Vullo ... centrifuged (at 14 000 g, 10 min, °C) and washed twice each with four buffers (B-I: 0 .1% SDS, 1% Triton X -10 0, mm EDTA, 15 0 mm NaCl, 20 mm Tris ⁄ HCl, pH 8; B-II: 0 .1% SDS, 1% Triton X -10 0, mm EDTA,...
... obtain sN ¼ Nðr À 1 ðrN 1 þ 1 À 2rðrN À 1 bPT =Q ðr À 1 2 ðrN 1 À 1 HN HN ¼ sN ¼ bPT =Q þ r a þ b 15 10 N =1 B where 10 10 0 Random b1 13 Þ 0 .1 Stimulus strength, KT /PT 12 Þ so that the transition ... bn ¼ [in (A)]; a1 ¼ bN ¼ 0. 01, an ¼ (n „ 1) , bn ¼ (n „ N) [in (B)]; N ¼ 4, L0 ¼ L1 ¼ ¼ LN ) ¼ 0 .1, QN ¼ QN ) ¼ ¼ Q1 ¼ 0 .1 [in (A–B)] 10 54 FEBS Journal 274 (2007) 10 46 10 61 ª 2007 The Authors ... mechanism and N! for the random mechanism 10 48 dY0 ¼ Àa1 Y0 þ b1 Y1 dt dYn ¼ an Yn 1 À ðan 1 þ bn ÞYn dt þ bn 1 Yn 1 ; for n dYN ¼ aN YN 1 À bN YN dt ð2aÞ ð2bÞ N 1 ð2cÞ where an and bn are effective...
... (19 98) Structure and Function of Ó FEBS 2002 10 11 12 13 14 15 16 17 18 19 20 21 22 Mechanism of IRF-3 virus-dependent activation (Eur J Biochem 269) 615 1 the Interferon-b Enhanceosome Cold Spring ... presence of cotransfected p300 (1. 5 lg), WT or the indicated mutants, schematically represented in (F), DNR (D3 17 3), DBromo (D10 71 12 41) , DE1a (D1739 18 71) , DSRC (D2042– 215 7) [27] reduced transcriptional ... with GST-CBP1 )11 00, GST-CBP1892)24 41 or their combination did not result in any activation of the ISRE · 3CAT reporter (Fig 6B) The inability of GST-CBP1 )11 00 and GST-CBP1892)24 41, alone or in...
... Ó FEBS 2004 10 11 12 13 14 15 16 17 18 19 the mammary gland of lactating transgenic mice Secretion into the milk and purification of the HIP/PAP lectin Eur J Biochem 267, 16 65 16 71 Christa, L., ... before 10 lM brefeldin A was added to the culture medium At the end of incubation, cells lysed in buffer A (10 mM KH2PO4 pH 7.4, 15 0 mM NaCl, 10 mM EDTA, 1% (v/v) Triton X -10 0 and lgÆmL )1 aprotinin, ... kgt 11 DNA purification kit (Stratagene) Chang cells (CCL13, ATCC) seeded in 10 0 mm Petri dish were maintained in DMEM supplemented with 7% (v/v) fetal bovine serum, 10 0 lgÆmL )1 streptomycin and 10 0...
... 2000 12 0 Eicosanoid Protocols, edited by Elias A Lianos, 19 99 11 9 Chromatin Protocols, edited by Peter B Becker, 19 99 11 8 RNAProtein Interaction Protocols, edited by Susan R Haynes, 19 99 11 7 Electron ... interactions Electrophoresis 19 , 12 4 712 53 Cann, J R (19 98) Theoretical studies on the mobility-shift assay of proteinDNA complexes Electrophoresis 19 , 12 714 1 Cann, J R (19 89) Phenomenological theory ... J Vogel, 20 01 172 Calcium-Binding Protein Protocols, Volume 1: Reviews and Case Histories, edited by Hans J Vogel, 20 011 71 Proteoglycan Protocols, edited by Renato V Iozzo, 20 01 170 DNA Arrays:...