nemesys facilitates identification of gene function and genes essential for viability

... gene structure and density statistics are shown in Table The base composition of Br and At genes is very similar The average length of Br genes (ATG to stop codon) is 73% that of At genes This is ... [20] *Tandem duplicated genes were considered to be a single homolog †The degree of conservation was calculated by dividing the number of conserved genes by the total number of genes ‡Information ... understanding of crop evolution Integration of genomes based on sequence level comparisons can offer a platform for the correlation between specific genes and phenotypes, which is important for...

Ngày tải lên: 09/08/2014, 20:20

... the size of the assembled genome increased by 29 Mb (12% of the version 4.0 genome) as a result of additional sequencing reads and better assembly, a total of 76 Mb of sequence (32% of the genome) ... most of the conflicts (1.8% of chromosomal misassignment and 0.7% of local misordering) were attributable to mapping errors For the rat genome, the radiation hybrid map was consistent for 98% of ... detailed genetic map existed before the sequence of the genome, but of the 12,000 markers, only 2,605 were considered as ‘unambiguously’ mapped and were used to assess the accuracy of the assembly...

Ngày tải lên: 14/08/2014, 18:20

... intensity of the ethidium bromidestained band with the marker bands A total of 30–90 ng of DNA is recommended for direct sequencing of PCR products Analysis, sequencing and in vitro expression of PCR ... polymerase-directed synthesis of new DNA from a primer annealed to a single-strand template DNA molecule In general for PCR products the template will be doublestranded and is heat denatured and rapidly frozen ... genomic PCR has been performed using degenerate primers and where there is no unique internal sequence information available for the amplified fragment The inclusion of a generic primer site will...

Ngày tải lên: 25/10/2013, 22:20

... performed in triplicates Amplification conditions were 40 cycles with the following profile: 95 °C for 30 s, 60 °C for 30 s, and 72 °C for For each kind of tissue, standard curves were generated for ... (RpCAbr and RpCAtr) not appear to be membrane-bound isoforms The RpCAbr and the RpCAtr transcripts are phylogenetically related and both distant from the vertebrate membrane-bound (CAIV) isoforms, and ... Further functional and histological studies suggested the existence of several carbonic anhydrase isoforms in the trophosome tissue [16,17], indicating the possible existence of various CA isoforms...

Ngày tải lên: 18/02/2014, 16:20

... CNDP gene was first duplicated to form the original CNDP and the copied CNDP genes The copied CNDP gene was further duplicated to form ‘serum’ carnosinase and anserinase genes This second gene ... amino-acid sequences of vertebrate M20A genes including the gene of Nile tilapia anserinase and constructed the unrooted phylogenetic tree shown in Fig Gene sequences from ascidian and vertebrate animals ... shown) Cloning of anserinase cDNA PCR was performed using a set of primers (A and B) and the first-strand cDNA for 3¢ rapid amplification of cDNA ends (RACE), prepared from total RNA of 6004 Nile...

Ngày tải lên: 19/02/2014, 07:20

... the LIRs probably have essential functions and drive the speciation of primates, we studied the degree of conservation and species specification of the LIRs among orthologous genes from the mouse ... approximately 27% of the human genes are pseudogenes The occurrence of pseudogenes adjacent to LIRs is statistically significant (chi-square test; P < 0.0001) Sequence features of the human LIRs ... numbers of such groups and the members inside the groups are not large We identified 20 LIRs in genes encoding a novel protein similar to septin (NPS-Septin) and eight in genes encoding POTE The genes...

Ngày tải lên: 07/03/2014, 00:20

... representation of the mouse mt D-loop region encompassing the conserved sequence boxes I-III (CSB), tRNA genes, L and H strand promoters (LSP and HSP), L and H strand transcription start sites (LSS and ... downstream of the 16 S rRNA gene, and at the 5¢ end of the tRNALeu gene on mouse mt genome [15,16] Studies on S1 nuclease analysis, coupled with the 5¢- and 3¢-end mapping of D-loop H-strand coded ... The polarity of triangle indicates the direction of the transcription OL and OH represent the origin of L and H strand replication, respectively LSS, L strand start site; HSS, H strand start site...

Ngày tải lên: 08/03/2014, 08:20

... category of PPE, for example, a life jacket for a canoeing instructor DUTIES AND RESPONSIBILITIES Responsibility for the health and safety of employees/students and the implementation of this ... responsibilities of various individuals within the University STATUS OF THIS CODE This Code of Practice and standards is part of the University’s arrangements for bringing into effect the Health and Safety ... piece of equipment The detail and content of the record will vary depending upon the type and use of the PPE concerned For complex pieces of equipment this should contain the following information:...

Ngày tải lên: 08/03/2014, 13:20

... Site-directed mutagenesis of ricin A-chain and implications for the mechanism of action Proteins 10, 270–278 17 Kim YS & Robertus JD (1992) Analysis of several key active site residues of ricin A chain ... inactive isoform, and the observed activity may be due to ‘impurities’ of another highly active isoform A definitive answer to this question will be given by cDNA cloning, which is one of our objectives ... Overall structure of charybdin b-Strands are shown in blue and a-helices in red The structural elements are labeled as follows: b-strands 1–10 and helices A–J The N-termini and C-termini of the protein...

Ngày tải lên: 16/03/2014, 14:20

... level of understanding the biology of tumour and degeneration and enable to use our acquired knowledge of S100 structure and functions in developing strategies to modulate their activity for therapeutic ... account for the promiscuity of S100 proteins Therefore, systematic studies of the conformational changes and oligomerization of S100 proteins will be of critical importance in the development of potential ... crucial role in the formation of larger oligomeric species of S100 proteins, namely tetramers, hexamers and octamers These are, in many cases, essential for biological function and signalling: tetrameric...

Ngày tải lên: 23/03/2014, 03:20

... attachment to the surface of epithelial cells and the effacement of microvilli [5,6] The formation of these ‘attaching ⁄ effacing’ lesions is critical for the pathogenesis of EPEC and EHEC, because bacterial ... membrane of uninfected mammalian cells Linking actin pedestal formation and pathogenesis Actin pedestals and colonization It is well established that Tir is essential for intestinal colonization and ... because genetic deletion of IRSp53, RNAi-mediated silencing of IRTKS or overexpression of dominant negative truncations of either of these proteins inhibits EspFU recruitment and pedestal formation...

Ngày tải lên: 29/03/2014, 09:20

Ngày tải lên: 01/04/2014, 11:52

... types and procedures for operational handling of them is given Naturally, there is a link between fouling and how to allow for it in design, and some approaches are offered from a consideration of ... spacing of mm - typical of the plate and frame generation of exchangers An informative figure given by Shah (1983) shows the 'spread' of values and representative surfaces - mechanical and natural ... function of both geometrical compactness of the surface(s) and of the performance parameters independently of the surface Although the compactness and performance parameters appear in separated form...

Ngày tải lên: 02/04/2014, 15:23

Ngày tải lên: 02/04/2014, 15:26

... “Surface Production Operations” series for further details and additional information such as derivations of some of the equations, example problems and solutions and suggested test questions This ... Determination of external water leg height, H Calculate the height of the oil and the height of the water in the tank Ho ¼ height of oil outlet À height of interface level ¼ 23 À 10 ¼ 13 ft Hw ¼ height of ... Corrosiveness of the crude oil, produced water, and associated gas, Scaling tendencies of the produced water, Quantity of fluid to be treated and percent water in the fluid, Paraffin-forming tendencies of...

Ngày tải lên: 02/04/2014, 16:03

... professionals ■ Depth of information They typically contain information on thousands and even tens of thousands of investment managers and products covering a variety of asset classes and geographical ... can be used in letter for- Request for Information 33 mat for direct mailing/fax or for e-mail format (which I generally prefer) I also generally post an RFI to a web site and invite investment ... frequency, and format of evaluations ■ Format to place investment managers on warning ■ Absolute performance 10 ■ ■ ■ ■ ■ ■ ■ ■ BEFORE THE ANALYSIS Relative performance (benchmark and peer group)...

Ngày tải lên: 08/05/2014, 10:12

... the CFO and channel for the uplink of multiuser MIMO-OFDM systems in frequency selective fading channels An asymptotic CramerRao bound for joint CFO and channel estimation in the uplink of MIMO-Orthogonal ... function The new cost function is independent of the actual CFO values and is an accurate approximation of the original SIR-based cost function for small CFO values Using the new cost function, we obtain ... Both Frank and Zadoff sequences and S&H sequences exist for sequence length of N = K , where N is the length of the sequence and K is a positive integer, while Chu sequences exist for any integer...

Ngày tải lên: 21/06/2014, 05:20

... of (44) are functions of Nk ; however, the resulting Nk is not a function of P or SNR similar to [28] Recall that we have only considered the subsets of [K] of the form T = {k} For the rest of ... antennas of stage n and the last βn+1 antennas of stage n + of partition P2 , and Hnn S last is the β n+1 channel matrix between the last βn antennas of stage n and the last m antennas of stage n + of ... DM-tradeoff of our hybrid JEEMAS strategy and the strategy of [15] for M0 = 2, M1 = 4, M2 = and M0 = 3, M1 = 5, M2 = in Figures and For the case when βn = 0, ∀n, the achievable DMtradeoff of our hybrid...

Ngày tải lên: 21/06/2014, 20:20

Ngày tải lên: 24/06/2014, 09:26

... tự DNA lớn gene cho thấy khả ứng dụng phương pháp việc nghiêu cứu so sánh cấu trúc gene Các tác giả nhận định phương pháp không tốn kém, đơn giản, dễ áp dụng vào việc đọc trình tự gene plastid, ... trình tự gene từ DNA tổng số Kết nghiên cứu tiến hành cho thấy kỹ thuật có ưu điểm thời gian lẫn giá thành thực Phương pháp tập trung khái thác vùng mã hóa có độ bảo tồn cao chèn nằm gene plastid ... đó phải đọc toàn trình tự gene lục lạp từ dòng BAC plasmid Các công việc liên quan đến quy trình chuẩn bị phức tạp tách DNA lục lạp nhận diện dòng BAC đặc hiệu chứa gene lục lạp để đọc trình...

Ngày tải lên: 01/07/2014, 18:20